V-P and MR tests were done at the same time using white porcelain board.The results showed that the white porcelain method was simple and convenient compared to the traditional method.

OBJECTIVE To investigate the distribution and the drug resistance of pathogens in infective cases and to provide a foundation for reasonable application of antibacterials.METHODS The information of all bacterial cultures and susceptibility tests from the inpatients in the year of 2006 was analyzed.RESULTS A total of 336 strains of pathogens were isolated,and the pathogens causing lower respiratory tract infection rated the top(60.7%).The resistance rate of Acinetobacter baumannii was the highest in Gram-negative bacilli.It was 33.3% to imipenem,and more than 66.6% towards the other antibacterials.All Gram-positive cocci were sensitive to vancomycin.The resistance rate of Enterococcus and Staphylococcus aureus was more than 75% to the most antibacterials.CONCLUSIONS Inspecting pathogens and strengthening susceptibility tests are very important in reducing drug abuse,decreasing the resistance rate and raising the cure rate in hospital.

Objective To investigate the effects of therapeutic hypercapnia on acute pulmonary allograft rejection induced by macrophages in rats.Methods Twenty-four adult male Wistar rats and 12 adult male Sprague-Dawley rats,weighing 250-280 g,were used in this study.The recipient rats were randomly divided into 3 groups using a random number table (n =6 each):syngraft group (group S),allograft group (group A) and therapeutic hypercapnia group (group H).In group S,Wistar rats served as donors and recipients,while in A and H groups,Sprague-Dawley rats served as donors and Wistar rats served as recipients.Orthotopic left lung transplantation was performed using the cuff technique.After transplantation,the rats inhaled 50％ N2-50％ O2 for 90 min during reperfusion in S and A groups,while in group H the rats inhaled N2-O2-CO2 for 90 min during reperfusion and PaCO2 was maintained at 80-100 mm Hg and O2 concentration in inspired air at 48％-50％ by adjusting the concentrations of the three gases.At 7 days after operation,the arterial blood sample was collected for blood gas analysis and for determination of serum concentrations of tumor necrosis factor α (TNF-α) and interferon γ (IFN-γ)by ELISA.The oxygenation index was calculated.Then the rats were sacrificed,and the transplanted lungs were removed for microscopic examination and for detection of infiltration of macrophages (by immunohistochemistry)and cell apoptosis (by using TUNEL) in lung tissues.The rejection was scored and apoptotic index was calculated.Results Compared with group S,PaCO2,serum concentrations of TNF-α and IFN-γ,rejection score,the number of macrophages and apoptotic index were significantly increased,and oxygenation index was decreased in group A (P ＜ 0.05).Compared with group A,pH value and oxygenation index were significantly increased,and serum concentrations of TNF-α and IFN-γ,rejection score,the number of macrophages and apoptotic index were decreased in group H (P ＜ 0.05).Conclusion Therapeutic hypercapnia can reduce macrophage-induced acute pulmonary allograft rejection possibly through inhibiting the inflammatory responses and cell apoptosis.

Objective To study the expression of tight junction factors in human placental tissues derived from assisted reproductive technology (ART) and natural pregnancy and its role in placental barrier.Methods Ten placental samples were collected from the women who had undergone ART treatment and 11 placenta were collected from control group.Transmission electron microscope (TEM) examination was utilized to detect the morphology of placental tight junctions.The mRNA of claudin (CLDN) 1,CLDN4,CLDN5,CLDN8,zonula occudens (ZO) 1 was detected by real-time PCR and the protein of CLDN4,CLDN8 and occludin (OCLN) were measured by western blot.Results TEM microscopy results showed that placenta samples derived both ART and control placenta had normal microscopic histological features of tight junctions,localized in the apical part of the syncytium and also between the cell-cell contacts of fetal blood vessel endothelial.The expression level of CLDN4 mRNA were 0.87 ±0.17 in ART group and 1.18 ± 0.30 in control group,respectively.The expression level of CLDN8 mRNA were 3.25 ± 2.32 in ART group and 1.08±0.41 in control group,respectively.The mRNA level of CLDN4 and CLDN8 were significantly differentially expressed in ART derived placenta when compared with control groups.The expression level of CLDN1,CLDN5,OCLN and ZO1 mRNA were 0.49 ± 0.44,0.80 ± 0.20,0.92 ± 0.18 in ART group and 1.09±0.82,1.21 ±0.78,0.80± 0.27 in control group,respectively,in which there were no significant differences between two groups.Western Blot analysis showed the protein levels of tight junctions CLDN4,CLDN8 and OCLN did not differ between groups.Conclusions Tight junction factors were expressed in human placental tissues.Tight junction derived from ATR platenta might have mild dysfunction.

Objective To explore the relationship between the status of the cervical lymphatic metastasis of papillary thyroid carcinoma(PTC)at cNo stage and tumor recurrence and the patient' s prognosis.Methods The clinical data of 498 cNo PTC patients admitted from 1986 to 1990 were retrospectively analyzed.Results All the patients were followed up for more than 10 years.16 patients died of PTC,among them 3 for metastasis,13 for local recurrence.The total cervical lymphatic metastasis rate in these 498 patients was 52.2％,in 260 cases in which detailed data were available: rate was 14.1％ in zone Ⅱ,27.1％ in zone Ⅲ,24.6％ in zone Ⅳ,12.5％ in zone Ⅴ,and 20.1％ in zone Ⅵ respectively.According to the appearances of microscopic pathology,the cervical lymphatic metastasis rates in 498 cases of cNo PTC were collected as following: uncapsuled group was 65.6％,extracapsular group was 64.5％,extralobal group was 56.5％,involved the surrounding tissue and structure group was 52.0％,focal cancerous focus group was 33.3％,latent sclerosis group was 26.9％,encapsuled and introcapsule group was 0.The cervical recurrence rate was only 2％ in the complete function neck dissection group and 9.9％ in the selective center neck dissection group.Conclusions In cNo PTC the most common cause of death was local recurrence.Prophylactic function complete neck dissection should be performed for cNo PTC cases of highly invasive type such as without tumor capsule,extracapsular,extralobal or surrounding tissue invasion.

Objective To observe the effect of moxa smoke (Artemisia vulgaris) on amino acids neurotransmitters in the brain of senescence accelerated mouse (SAMP8) and explore the anti-aging effect of moxa smoke. Methods Totally 70 SAMP8 were randomized into one model control group and 6 intervention groups, and 10 SAMR1 mice were used as normal control group. There were low, medium and high concentrations of moxa smoke during intervention. Moxa smoke intervention was performed 15 min/30 min each day for 28 days. High performance liquid chromatographic method was used to determine the levels of Glu, Asp and GABA in the brain of the mice. Results Compared with the normal control group, the levels of Asp and Glu in the model control group were significantly higher, while the GABA was significantly lower. The levels of Asp and Glu in 6 intervention groups were significantly lower than those in the model control group, while GABA was significantly higher than or the same as the model control group. There was no significant difference among different intervention groups in the levels of Asp and Glu, while the 30 min-medium concentration moxa smoke had the most significant effect in increasing the level of GABA. Conclusion Moxa smoke intervention could decrease the high level of Asp and Glu, and increase the low level of GABA in SAMP8. It could achieve the effect of anti-aging through adjusting the abnormal metabolism of amino acids in the brain.

Objective To investigate the killing effect and molecular mechanism of different doses of ~(125)I seed interstitial brachytherapy for human hepatoma HepG2 cells in nude mice transplanted tumor.Methods Twenty nude mice bearing human hepatoma HepG2 cells were randomly divided into 4 groups (each n=5) and given ~(125)I seed interstitial brachytherapy at different doses including 18.5 MBq ~(125)I,29.6 MBq ~(125)I,37.0 MBq ~(125)I and 0 (the control group) ,respectively.~(125)I seeds were percutaneously implanted into tumors,each mouse with 1 ~(125)I seed implanted.Twenty-eight days later,nude mice were sacrificed and routine pathological examination was performed,.The expression of Bcl-2 and Bax was detected with immunohistochemical methods.Results Pathological examination showed serious coagulation necrosis of tumor cells surrounded by extensive fibrosis in all treatment groups,whereas rich tumor cells with proliferative growth were observqed in the control group.Immunohistochemical analysis showed that the expression of Bax in all treatment group were higher than that in the control group.the expression of which was up-regulated with increasing doses of ~(125)I seed.The expression of Bcl-2 and the ratio of Bcl-2/Bax in all treatment groups were lower than those in the control group.the expression of which was down-regulated with increasing doses of ~(125)I seed.The expression of Bcl-2.Bax and the ratio of Bcl-2/Bax among treatment groups and control group had statistical difference (P＜9.05) ,so as the difference among treatment groups (P＜0.05) .Conclusion ~(125)I seed interstitial brachytherapy induces apoptosis in human hepatoma HepG2 cells by reducing the ratio of Bcl-2/Bax and thereby inhibits the growth of tumor in nude mice,and the extent positively correlates with dosage in a certain range.

Objective To evaluate the effects of ELISPOT (enzyme-link immunospot) test using different samples in diagnosing tuberculous pleurisy. Methods Using T-Spot-TB kit to detect interferon-γlevel in pleural effusion and periph?eral blood from 164 patients with tuberculous pleural effusion and 102 patients without tuberculous pleural effusion. Number of spot forming cells (SFCs) as well as the specificity and sensitivity of the tests were compared between these two methods (ELISPOT using leural effusion or peripheral blood). Results The area under the ROC curve was 0.947 in pleural effusion Elispot test while it was 0.905 in peripheral blood Elispot test. The sensitivity of pleural effusion ELISPOT test in diagnosis of tuberculous pleurisy (95.1%) was significantly higher than that of peripheral blood ELISPOT test (89.0%). What’s more, the specificity of pleural effusion ELISPOT test in diagnosis of tuberculous pleurisy (90.2%) was higher than that in diagno?sis of peripheral blood ELISPOT test (88.2%). Conclusion The pleural effusion ELISPOT test is more valuable than periph?eral blood ELISPOT in the diagnosis of tuberculous pleuritis.

BACKGROUND: BALB/c and C57BL/6 mice are two inbred strains, but after voluntary movement, there is no report on how to scientifical y reasonably select behavioral experiment methods and indicators and to evaluate the learning and memory abilities of mice.
OBJECTIVE: To analyze and compare the behavioral indicators between BALB/c and C57BL/6 mice fol owing voluntary wheel running, to explore the effect of exercise on learning and memory, and to provide a reference for selecting reasonable behavioral indicators.
METHODS: 2.5-month-old BALB/c and C57BL/6 mice were randomly divided into control and voluntary wheel running groups. Independent running wheel movement of mice was recorded with VitalView system. 4 weeks later, newborn neurons were labeled via DCX immunofluorescence. Spatial learning, memory and exploration abilities were compared through new arm test, new object recognition test and Morris water maze test.
RESULTS AND CONCLUSION: (1) The mean spontaneous activity of BALB/c mice daily was 2.56 fold of that of C57BL/6 mice during wheel running (P < 0.001). (2) Hippocampal DCX-positive cel s in exercised BALB/c and C57BL/6 mice were more than those in control group. (3) Meantime, exhibited by higher frequencies to explore new arm or object, and longer time and distance of moving around them, the learning and exploring capability was improved after exercising (P < 0.001), especial y in BALB/c mice. (4) Wheel running C57BL/6 mice exhibited progressed spatial learning and memory abilities compared with control mice in Morris water maze test, characterized by decreased latency to target, elevated target crossings and longer time or distance in quadrant zone (P < 0.05). However, there was no significant difference between wheel running and control BALB/c mice. Taken these data together, voluntary wheel running contributed to hippocampal neurogenesis of BALB/c and C57BL/6 mice, accompanied by the change of learning and memory capability, which could be detected properly via both new arm test and new object recognition test, but for Morris water maze test, C57BL/6 mice might be superior to BALB/c mice.

Objective:To investigate the animal model of guinea pig chronic heart failure and compensatory hypertrophy. Methods:Thirteen chronic heart failure models were made by placing a constricting band around the ascending aorta of guinea pigs,raised in the same house of 10 normal guinea pigs. The contrasting indexes in 6 weeks include: clinical features, hemodynamics, the mass ratio of ventricular over body, the width of left ventriculat hypertrophy. Results:One model died, 4 models without dyspnea showed increasing in left ventricular pressure and the mass ratio of ventricular over body, whereas the left ventricular end diastolic pressure showecl no significant different. Eight models with dyspnea showed striking increasing in left ventricular pressure and left ventricular end diastolic pressure, as well as the mass ratio of ventricular over body and the width of left ventricular hypertrophy. Conclusion:The animal model of guinea pig chronic heart failure and compensatory hypertrophy may be formed by constricting the ascending aortas of guinea pigs for 6 weeks.