Objective To study the relationship between the changes of cytosine,thymine,guanine(CTG)repeat numbers and brain stem BAEP,SEP and VEP in patients with myotonic dystrophy(DM) and their family members.Methods The repeat numbers of CTG,BAEP, SEP and VEP of DM gene were determined by PCR amplification and DNA hybridization in 5 patients with DM and 16 members from 3 families.Results The repeat numbers of CTG in 10 normal persons were 30,BAEP,SEP and VEP were normal.The repeat numbers of CTG in 5 patients with DM were over 85,two cases of them were over 1605,they were significantly higher than the normal persons.4 cases in 16 family members were normal,CTG repeat numbers of 12 cases were over normal genes,CTG repeat numbers were related to clinical symptom, BAEP,SEP and VEP.Conclusion The gene diagnosis of DM was found to be consistent with its clinical symptom,the changes of BAEP,SEP and VEP.

Objective　To study the relationship between the changes of cytosine，thymine，guanine（CTG）repeat numbers and brain stem BAEP,SEP and VEP in patients with myotonic dystrophy（DM） and their family members.Methods　The repeat numbers of CTG，BAEP， SEP and VEP of DM gene were determined by PCR amplification and DNA hybridization in 5 patients with DM and 16 members from 3 families.Results　The repeat numbers of CTG in 10 normal persons were 30，BAEP，SEP and VEP were normal.The repeat numbers of CTG in 5 patients with DM were over 85，two cases of them were over 1605，they were significantly higher than the normal persons.4 cases in 16 family members were normal,CTG repeat numbers of 12 cases were over normal genes,CTG repeat numbers were related to clinical symptom, BAEP，SEP and VEP.Conclusion　The gene diagnosis of DM was found to be consistent with its clinical symptom，the changes of BAEP，SEP and VEP.

Dental Occlusion ; Esthetics, Dental ; Humans ; Mechanoreceptors ; Temporomandibular Joint

Objective: To study the biological properties of a decellular human tissue engineering homograft aortic valves conduits. Methods: (1) Human homograft aortic valves conduits were treated with the pH 8 0 hypotonic tris buffer for 12h. (2) Then with pH 8 0 isotonic buffer added with the 1% DOA (deoxycholic acid) for 24h. (3) At last, a pH 7 6 isotonic buffer with the DNAase 200?g/ml, NAase 20 ?g/ml was applied. The histology, water content, thickness, denaturation temperature, DNA content, collagen contents were examined. Results: The valves, the wall and the muscle layer of the human homograft aortic valves conduits were decellulared completely compared with the standard crypreserved human homograft aortic valves. Water content in the decellular aortic wall was increased significantly (75 44?1 84 vs. 82 05?0 71,P

Objective:To explore the immunosuppression effects and tocolysis of fructus polygoni orientalis on abortion mice induced by LPS.Methods:Mice of Kunming (55 mice) were divided into control group (group A ,10 mice) and experimental group. Experimental group were divided into group B ( intraperitoneal injection LPS ) , group C ( ingestion fructus polygoni orientalis ) , and group D (ingestion fructus polygoni orientalis and intraperitoneal injection LPS ),each group of 15 mice.Then the pregnancy results were observed ,the positive of α-NAE+and the varity of CD 14+and CD204+macrophages ,TNF-αin the uteri were identified by enzyme-histochemistry ,immunohistochemistry and ELISA.Results:The abortion rate and the embryo resorbing rate were all 100%( P<0.01 ) in group B.But there was the decreased abortion rate of 13.33% in group D .The embryo resorbing rate decreased to 10.39%.The number and positive cell area of α-NAE+and CD14+macrophages in the uteri of gestation mice of group B was greatly increased comparing with group A ( P<0.01 ) .These effects outside of myometrium of group D were remarkably increased comparing with group A (P<0.01),but there was no distinct difference in the inside of myometrium and function layer.The number and positive cell area of CD204+macrophages in group C and D was greatly increased comparing with group A and B ( P<0.01 ) .The TNF-αcontents in the uteri of mice in group B were greatly increased comparing with group A (P<0.01),but the positive cell area of CD14,CD204 were close to normal levels in group D.Conclusion:The effect of miscarriage induced by LPS is antagonized by fructus polygoni orientalis through inhibiting the phenotype ,activity and function characteristics of macrophages in the uteri of gravidity mice.

Objective:To analyse the imaging featrues of intractranial tuberculoma and improve the diagnostic accuracy.Methods:31 patients with clinical characteritics and pathological proved intracranial tuberculomas were studied retrospectively.Results:"egg-shell"calcification were the feature of giant calcified and ossified tuberculoma.CT scaning were single and multiple nodular lesion.In the contrast enhancing CT scaning,plate shaped or ring form shadows were shown.MRI were provided hypointense on T1WI and hyperintense on T2WI.The rim homogeneous enhancement were showd in the Gd-DTPA.Conclusion:The diagnosis of typical intracranial tuberculomas can be made.After antituberculosis chemotherapy,CT and MRI can help made differsntial diagnosis.Operative indications should be select strictly.

BACKGROUND: The human homograft valve has been widely used in the operation of the valve replacement and congenital heart disease. However, reports concerning pulmonary valve biomechanical suitability as a long-term substitute for aortic valve are limited.OBJECTIVE: To compare the biomechanical properties between human aortic and pulmonary valves conserved with ultra low temperature in liquid nitrogen.METHODS: A contrast observation trial was conducted in the laboratory of Congenital Heart Disease of Fuwai Hospital from March to July in 2004. Six samples of heart aorta valves and pulmonary valves were collected from the cadaveric heart within 6 hours, and the experimental procedure was disposed according to ethical criteria. The biomechanical properties of the circumferential direction of the valve were investigated using uniaxial tensile tests. The thickness of the valve was measured by HD-10 thickness instruments.RESULTS AND CONCLUSIONS: Compared to human aortic valves, the thickness of the human pulmonary valves was thinner significantly, but the stress-strain, tensile strength and the maximum tangent modulus E (the slope of linear segment) in the curve had no significant difference (P> 0.05). Biomechanical differences between the aortic and pulmonary valve are minimal, in respect, pulmonary valve can be considered biomechanically suitable for aortic valve replacement.

Objective To study the correlation among pathological,clinical and the imaging features(CT and MRI) of the Rathke cleft cysts.Methods CT,MRI and clinical findings of Rathke cleft cysts in 43 patients confirmed by operation and pathology were retrospectively studied.Results 27 cysts located at intrasella and suprasella and 16 cysts entirely located at intrasella.Cysts were round or oval in shape with definite borders.The size of 30 cysts exceed 10mm in diameter.On CT scans reviewed,the cysts were low density in 9 cases,hyper-density in 9 cases and isodensity or mixed density in 8 cases.The cyst's wall with calcification was seen in one.On postcontrast CT scans,6 cases showed circular and peripheral cyst's wall enhancement and others were no enhancement.On MR imaging,the lesions were low or isodense on T1WI and hyper-intensity on T2WI in 18 cases,both were high signal intensity on T1WI and T2WI in 6 cases,high signal intensity on T1WI and mixed signal intensity on T2WI in 4 cases.An intracystic nodule having high signal intensity on T1WI,and low or mixed signal intensity on T2WI was observed in 4 cases.On contrast-enhanced MR imaging,enhancement of the cyst's wall was shown in 9 cases.During surgery,the lesions were noted to have a cyst of semisolid consistency,and cystic contents were described from CSF-like clear fluid in 10 cases,jellied-like brown mucoid fluid in 15 cases,caseous-like mucoid fluid in 12 cases,machine oil-like mucoid fluid in 6 cases.At histopathology,a part of cystic fluid included cholesterol crystal and necrotic debris.Cholesterol clefts and hemosiderin pigment,and granuloma were shown by staining with HE in 11 cases.The PAS staining was positive in 16 cases,cystic fluid contained partial mucopolysaccharides and protein.Conclusion Typical Rathke cysts can be dignosised in the preoperative,the findings of CT and MRI are not specific in atypical cysts.The imaging features were different with the fluid component of Rathke cleft cysts..

Objective To study the therapeutic effects of low frequency repetitive transcranial magnetic stimulation(rTMS)in cranial dystonia.Methods Twenty cranial dystoina patients were treated with low frequency rTMS.Their motor threshold,cortical silent period(CSP)were evaluated before and after the rTMS and after 1,2,6 months as well as the spares and Toronto Western Spasmodic Torticollis Rating Scale(TWSTRS)to evaluate the effects of rTMS in the treatment of cranial dvstonia.Results The patients scored(23.5±14.0)significantly lower after l and 2 months(17.6 ±14.3,18.5±14.2,t=2.632,2.149.both P<0.05).But there was an increasing tendeney of the score after 2 months.The 2-month efficient rate of low-frequency rTMS Was 60%(12/20),yet the long-term effect of rTMS was still to be studied.There was a very significant improvment of relaxed(46.5%±7.3%vs49.9%±9.2%,t=-3.235.P<0.05)and active threshold(40.2%±5.9%/)5 43.9%±8.8%,t=-2.339,P<0.05),prolongation of CSP((96.1±24.5)ms vs(121.6±27.7)ms,t=-7.223,P=0.000).Conclusion The low frequency rTMS is efficient to relieve the clinical symptoms of cranial dystonia.

Objective To investigate the protective effect of continuous intravenous infusion of Isoproterenol (ISO)on myocardial mitochondria of early septic rats and the corresponding mechanism.Methods Thirty Sprague Dawley (SD)rats were randomly divided into 5 groups (6 cases per group):control group,endotoxin group,ISO small-dose group,ISO medium-dose group and ISO large-dose group.Endotoxin group and ISO intervene group received same management apart from drug intervention:receiving intravenous injection of lipopolysaccharide (LPS)10 mg/kg followed by an continuous intravenous infusion of 9 g/L saline 1 mL/h or ISO 0.06 μg/(kg · min),0.30 μg/(kg · min)and 0.60 μg/(kg · min).Control group received intraperitoneal injection and continuous intravenous infusion with the same amount of 9 g/L saline.The primary endpoint of the study was 24 hours after injection of 9 g/L saline or LPS.Serum creatine kinase (CK) and creatine kinase isoenzyme (CK-MB),oxidative and nitrosative stress levels and swelling of isolated heart mitochondrion were detected.The pathological changes of the myocardium and morphologic changes of the heart mitochondria were observed through light microscope and scanning electron microscope,respectively.Results The levels of CK,CK-MB,nitric oxide (NO) content,inducible nitric oxide synthase (iNOS) activity and malondialdehyde (MDA)content in endotoxin group were increased compared with control group (all P ＜ 0.05),while the superoxide dismutase (SOD) activity decreased [(11.543 ± 1.080) U/mg prot vs (9.892 ±0.815) U/mg prot,P ＜0.05].The morphology of the heart mitochondria significantly changed (such as swelling,disordered arrangement,crest fracture,fusion and cavitations,and so on).ISO intervention significantly decreased the levels of CK,CK-MB and mitochondrial swelling (all P ＜ 0.05) and increased the SOD activity (all P ＜ 0.05).The levels of NO content,iNOS activity and MDA content were significantly decreased in small-dose group [(10.823 ± 2.240) μmol/g prot vs (7.917 ± 2.203) μmol/g prot,(0.045 ± 0.008) U/mg prot vs (0.033 ± 0.003) U/mg prot,(1.663 ± 0.618) mmol/mg prot vs (0.768 ± 0.312) mmol/mg prot,all P ＜ 0.05],while the levels of iNOS activity and MDA content were significantly increased in medium-and large-dose group (all P ＜ 0.05) ; compared with medium-dose group,the degree of mitochondrial swelling in large-dose group increased (1.160 ± 0.186 vs 1.393 ± 0.128,P ＜ 0.05).The pathological changes of the myocardium mitochondria significantly improved.Conclusions The myocardium and myocardial mitochondria of early septic rats were damaged,continuous intravenous infusion of low-dose ISO revealed protective effect on these damages,and the corresponding mechanism may relate to the decrease of the oxidative and nitrosative stress.