1.Clinical pathway management in the patients undergoing leiomyoma of uterus
Fang XU ; Mei CUI ; Zengping HAO
International Journal of Surgery 2009;36(8):536-538
Objective Investigate the effect of applying clinical pathway on the patients undergoing leiomyoma of uterus. Methods As an experimental group of patients, 50 cases of uterine leiomyoma are treated with clinical pathway. By contrast, another 100 cases are treated with the traditional medical care as control group. Results Waiting time for surgery and hospital stay of the experimental group are less than the control group; and the average hospitalization cost of experimental group is lower than the control group (P< 0.01). Conclusions Application of clinical pathway management will regulate the activities of medical treatment, ensure the quality of health care, improve the feeling of patients, increase efficiency of hospital services, reduce the financial burden of patients, increase the hospital social benefits.
2.Isolation and culture of primary microvascular endothelial cells from mouse brains
Zuohui ZHANG ; Chen CHEN ; Hao CHEN ; Guiyun CUI ; Fang HUA
Chinese Journal of Tissue Engineering Research 2016;20(51):7666-7671
BACKGROUND:Brain microvascular endothelial cel s (BMECs) are important tools in the field of neuroscience research;therefore, how to obtain highly purified BMECs is a key and difficulty in vitro.
OBJECTIVE:To develop a simple method of isolating and culturing highly purified BMECs.
METHODS:C57BL/6 mice aged 6-8 weeks old were selected, and microvessels were obtained using enzyme digestion and gradient centrifugation. Further, endothelia cel s were purified by certain drugs, fol owed by identified by CD31 and GFAP immunofluorescence staining. The expression of Claudin-5 was detected using immunofluorescence staining with anti-Claudin-5 antibody.
RESULTS AND CONCLUSION:Mouse BMECs grew and arranged in spiral or cobblestone-like. Immunofluorescence staining showed that the purity of BMECs reached above 99%and Claudin-5 was highly expressed. In conclusion, a simple method of easy accessibility is developed to obtain highly purified primary mouse BMECs.
3.Clinical efficacy of Chinese herbal retention enema combined with intrauterine douching for patients with endometritis.
Mei-mei WANG ; Cui-fang HAO ; Hong-chu BAO
Chinese Journal of Integrated Traditional and Western Medicine 2011;31(5):639-642
OBJECTIVETo study the clinical efficacy of Chinese herbal retention enema combined with intrauterine douching for patients with endometritis diagnosed by hysteroscopy. They failed in in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI)-embryo transfer (ET) treatment.
METHODS131 patients received hysteroscopy after they failed in routine IVF/ICSI-ET treatment. Of them, 66 patients diagnosed as endometritis were enrolled as the test group and 65 patients without endometritis were enrolled as the control group. Chinese herbal retention enema combined with intrauterine douching was performed on patients in the test group before the next IVF/ICSI, while direct IVF/ICSI was performed on those in the control group. The embryo implantation rate and the clinical pregnancy rate were compared between the two groups.
RESULTSThe clinical pregnancy rate and the embryo implantation rate were 48.5% and 24.2% respectively, while they were 29.2% and 14.9% respectively in the control group, showing significant difference (P < 0.05).
CONCLUSIONChinese herbal retention enema combined with intrauterine douching could improve the embryo implantation rate and the clinical pregnancy in patients with endometritis.
Adult ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Embryo Transfer ; Endometritis ; therapy ; Enema ; methods ; Female ; Fertilization in Vitro ; Humans ; Hysteroscopy ; methods ; Pregnancy ; Pregnancy Rate ; Sperm Injections, Intracytoplasmic ; Therapeutic Irrigation ; methods ; Treatment Failure
4.Effect of jiangya capsule on levels of serum nitric oxide synthase activity, malondialdehyde, plasma neuropeptide Y and homocysteine in patients with senile simple systolic hypertension.
Chinese Journal of Integrated Traditional and Western Medicine 2003;23(12):898-901
OBJECTIVETo observe the effect of Jiangya Capsule (JYC) on serum levels of nitric oxide synthase (NOS) activity and malondialdehyde (MDA), and plasma concentration of neuropeptide Y (NPY) and homocysteine (Hcy) in patients with senile simple systolic hypertension.
METHODSRandomized, double-blinded controlled method was adopted, the patients were divided into two groups, the treated group treated with JYC and the control group treated with Nimodipine. Changes of blood pressure, serum level of NOS and MDA, and plasma concentration of NPY and Hcy were observed.
RESULTSThe effect of lowering blood pressure in the two groups were similar with insignificant difference (u = 0.24, P > 0.05). The total effective ratio in alleviating TCM syndrome was 90% (18/20) in the treated group and 60% (9/15) in the control group, comparison between them showed significant difference (u = 2.18, P < 0.05). After treatment, the NOS activity increased and MDA, NPY and Hcy levels decreased in the treated group, which showed significant difference as compared with those before treatment, and the improvement were better than those in the control group (all P < 0.05 or P < 0.01). While in the control group after treatment, only level of MDA was lowered significantly (P < 0.05).
CONCLUSIONJYC has good effect in treating senile simple systolic hypertension, it also could increase the NOS activity, lower the serum MDA level, obviously reduce the plasma concentration of Hcy and NPY.
Aged ; Capsules ; Double-Blind Method ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Homocysteine ; blood ; Humans ; Hypertension ; blood ; drug therapy ; Male ; Malondialdehyde ; blood ; Middle Aged ; Neuropeptide Y ; blood ; Nitric Oxide Synthase ; blood ; Phytotherapy
5.Deficiency in Na-K-2Cl co-transporter impaired hearing and balance in mice
Hanqi CHU ; Hao XIONG ; Fang HAN ; Zhengong WU ; Xiaowen HUANG ; Yonghua CUI
Chinese Journal of Pathophysiology 1999;0(09):-
AIM:We generated transgenic mice of NKCC1-/-(homozygous mutant),NKCC1+/-(heterozygous)and NKCC1+/+(wild-type)that have a targeted disruption in the NKCC1 gene to investigate the role of Na-K-2Cl(NKCC1)channel in auditory function of the inner ear.METHODS:Hearing threshold and endocochlear potential(EP)were measured in the NKCC1-/-,NKCC1+/-and NKCC1+/+ mice by auditory brainstem response(ABR)and EP recordings,respectively.The inner ears of the mice were removed and examined morphologically with the light microscope.RESULTS:The auditory function of NKCC1+/+ mice was normal,the mean value for ABR thresholds in response to click sound was [(23.13?3.78)dB,SPL],EP was(98?16)mV.The mean value for ABR thresholds to click sound was elevated in NKCC1+/-mice [(38.49?12.29)dB,SPL],relative to that significantly increased in NKCC1+/+ mice(P
6.Application of reduced glutathione in protecting liver function in HCC patients after interventional therapy: a clinical study
Lin FANG ; Ning WEI ; Hao XU ; Maoheng ZU ; Yuming GU ; Qingqiao ZHANG ; Wei XU ; Yanfeng CUI ; Mengqi ZHANG
Journal of Interventional Radiology 2017;26(2):169-172
Objective To evaluate the clinical effect of reduced glutathione in protecting the liver function for patients with hepatocellular carcinoma (HCC) after receiving international therapy.Methods The clinical data of 110 HCC patients,who were admitted to authors' hospital during the period from January 2010 to June 2013,were retrospectively analyzed.According to the random number table method the patients were divided into the control group (n=50) and the treatment group (n=60),and transcatheter arterial chemoembolzation (TACE) and transcatheter arterial infusion (TAI) were carried out in all patients.After the treatment,intravenous infusion of 2.0 g vitamin C,0.2 g vitamin B6,2.0 g inosine,and 250 ml branched chain amino acid was employed per day for one week for the patients of both groups,and additional intravenous dripping of 1.8 g reduced glutathione (added in 250 ml of 5% glucose solution) was adopted for the patients of the treatment group.Various liver function indexes were tested before and one week after the treatment,the results were compared between the two groups.Results Before the treatment,no obvious differences in the hepatic functions and in the clinical condition existed between the two groups.One week after the treatment,in both groups the aminotransferase (ALT and AST) and total bilirubin (TBIL) were significantly increased when compared with the preoperative data (P<0.05),while the serum pre-albumin (PA) was remarkably decreased when compared with the preoperative data (P<0.05);these changes of various indexes in the treatment group were apparently better than those in the control group (P<0.05).Conclusion The use of reduced glutathione can effectively relieve the liver function damage caused by interventional therapy in HCC patients,therefore,reduced glutathione has a very good clinical application value.
7.Interpretation of consensus guidelines for diagnosis and treatment of aromatic L-amino acid decarboxylase deficiency
Fang ZHOU ; Yuxia CUI ; Hao ZHOU
Chinese Journal of Applied Clinical Pediatrics 2020;35(11):805-809
Aromatic L-amino acid decarboxylase deficiency(AADCD) is a rare early-onset autosomal recessive genetic neurometabolic disease which can be treated with target therapy.To find, diagnose and treat at its early stage is important to improving the prognosis.The international neurotransmitter-related diseases working group (iNTD) developed the consensus guidelines for the diagnosis and treatment of AADCD in 2017, which provided reco-mmendations based on the consensus to facilitate the diagnosis, treatment and long-term management of patients.This article interpreted and summarized the clinical symptoms and treatment of AADCD according to the consensus, in order to raise pediatric clinicians′ awareness and treatment level of the disease.
8.Interpretation of outpatient respiratory management of infants, children, and adolescents with post-prematurity respiratory disease: an official American Thoracic Society clinical practice guideline
Fang ZHOU ; Yuxia CUI ; Xike WANG ; Hao ZHOU
Chinese Journal of Applied Clinical Pediatrics 2022;37(11):801-805
In recent years, the management of respiratory diseases related to preterm birth has received extensive attention.In 2021, the American Thoracic Society brought together multidisciplinary experts in respiratory, neonato-logy, otolaryngology, sleep medicine, radiology and nursing specialties to develop Guidelines for outpatient respiratory management in infants, children, and adolescents with post-preterm respiratory disease (hereinafter referred to as the " Guideline" ), aiming to provide evidence-based medical evidence for standardized outpatient management of respiratory diseases associated with preterm birth at different ages.The Guideline was interpreted and summarized so that pediatric clinicians could correctly diagnose and treat these diseases, and understand and implement standardized outpatient management on the basis of evidence.
9.Correlation analysis between HBV Pre-S1 antigen with HBeAg and HBV DNA
Xiao-Jing HOU ; Yan LIANG ; Feng-Chun HE ; Jie CHEN ; Yan-Fang CUI ; Ren-Qian ZHONG ; Hao WANG ;
Academic Journal of Second Military Medical University 1999;0(12):-
Objective:To study the correlation between HBV Pre-S1 antigen,HBeAg levels and HBV DNA copies,so as to assess the clinical value of Pre-S1 in detection of HBV replication.Methods:A total of 363 HBsAg-positive samples were col- lected.The levels of Pre-S1 antigen,HBeAg and HBV DNA copies were determined by ELISA,time-resolved immuno-fluores- cent method and real-time fluorescent quantitative PCR(FQ-PCR),respectively.The correlation between the determination re- sults was analyzed.Results:Pre-S1 antigen level was correlated with the level of HBeAg(X~2=94.4,P
10.Construction of T vectors based on Xcm I recognition site and optimization of PCR fragments for ligation.
Yi-qiao ZHANG ; Yan-fang ZHANG ; Chao-liang LONG ; Chun-yue LI ; Xue-hui LONG ; Wen-yu CUI ; Hao ZHANG ; Hai WANG
Chinese Journal of Applied Physiology 2016;32(1):46-50
OBJECTIVETo construct T vectors based on Xcm I recognition site and optimize the PCR fragments for its ligation.
METHODSWe firstly cloned the human histone H4 cDNA containing one Xcm I recognition site at both its 5' and 3' end into pCDNA 3.0 vector and then generated T vector with pCDNA 3.0 backbone by cutting the recombinant plasmid with Xcm I. To increase the ligation efficiency, the primers were firstly phosphorylated before DNA fragments amplification and then the PCR products were treated with Taq DNA polymerase and dATP after PCR amplification. Two DNA fragments with the length of 312 bp and 1 329 bp were ligated to it and the ligation mixture was transformed into E. coli DH5α competent cells and the positive rates of the transformants were evaluated by PCR and DNA agarose gel electrophoresis.
RESULTSOur results showed that the T vector produced by our method could ligate to the target DNA fragments with high efficiency. Besides, the phosphorylation state of the primers used for PCR amplification is also an important factor determining the cloning efficiency. What's more, as for longer DNA fragments, retreatment with Taq DNA polymerase and dATP after PCR amplification and purification could improve the ligation efficiency significantly.
CONCLUSIONOur protocol may overcome the dependence on blue/white screening to get positive clones and provide a potent way to generate T vectors and ligate them to the target PCR fragment.
Cloning, Molecular ; DNA, Complementary ; genetics ; Escherichia coli ; genetics ; Genetic Vectors ; Histones ; genetics ; Humans ; Polymerase Chain Reaction ; methods