Cough is a common respiratory symptom in children.To evaluate severity of cough and the quality-of-life in children with chronic cough,cough scores,generic questionnaires and cough-specific health-related quality of life questionnaires have been developed.Different questionnaires have their own particular features,recently the validity and reliability of the cough-specific health-related quality of life questionnaires are supposed to be high in assessing quality of life,and have been proved to be quite valid and reliable tools.Studies have shown that chronic cough not only affects physical status in children,but also seriously affects their psychological health,social activities and family life negatively.Therefore,evaluating severity of cough and impacting on quality of life contributes to the clinical development of appropriate interventions.

Integrated traditional Chinese and Western medicine therapy for pancreatitis has been used since the 1960s.According to the clinical manifestations and traditional Chinese medicine (TCM) syndrome differentiation of pancreatitis,pancreatitis was named true heart pain with cold limbs caused by spleen disease.Syndrome differentiation of acute pancreatitis and treatment with modified Dachaihu decoction achieved good clinical effects.After the 1990s,the research focus of pancreatitis research was shifted to severe acute pancreatitis (SAP).The clinical course of SAP was divided into three phases,and different therapeutic regimens were given.Clinical studies achieved good therapeutic effects in terms of cure rate and fatality rate.After 2000,integrated traditional Chinese and Western medicine therapy for chronic pancreatitis has been promoted systemically,and the cooperation between traditional Chinese medicine,endoscopic techniques,and operative treatment helps to significantly improve pain control,nutritional status,and incidence of complications.

Objective:To observe the efficacy of different forms of triamcinolone acetonide( TA) in the treatment of buccal erosive oral lichen planus (EOLP). Methods:120 EOLP patients were randomly divided into 4 groups(n=30) and treated with Kangfuxin mouth-wash followed by topical ointment of( TA) , TA injection, TA mouth-wash and only Kangfuxin mouth-wash only, respectively. Before treatment, 1, 2 and 4 weeks after treatment, the pain (VAS score) and erosion area were measured, the effects were compared among groups. Results:The VAS scores of A, B and C groups were significantly lower than those of the control group after 1, 2 and 4 week treatment, and the total effective rate was significantly higher than that of the control group, respectively(P<0. 05). The VAS score was lower and total effective rate was higher in group A and group B than those in group C(P<0. 05), but there was no statistically sig-nificant difference between group A and B(P>0. 05). The average erosion area of group A and B was significantly smaller than that of the control group after 1, 2 and 4 week treatment(P<0. 05). There was no significant difference between group C and the control group after 1 and 2 week treatment(P>0. 05), but 4 weeks after treatment, the average area of erosion of group C was significantly smaller than that of the control group(P<0. 05). Conclusion:Triamcinolone acetonide is effective in the treatment of OLP and the oral oint-ment form is safe and convenient for OLP treatment.

Objective To explore the clinical features of steroid resistant nephrotic syndrome caused by NPHS2 gene mutation. Methods The clinical data of two pediatric patients with steroid resistant nephrotic syndrome were retrospectively analyzed. The pertinent literatures were reviewed. Results Both patients were male with onset age at 2 and 3 years old. The clinical features were heavy proteinuria, hypoalbuminemia, and hypercholesterolemia, which met the diagnostic criteria of nephrotic syndrome. Renal pathology found one patient with focal segmental glomerulosclerosis, and other with minimal-change. Both of them suffered from recurrent inguinal hernioplasty and one was accompanied with hypoplasia of left testis. Gene detection verified a NPHS2 gene mutation. Both of them were hormone resistant at the beginning of onset and later hormone combined with different kinds of immunosuppressive therapy was still ineffective. Both of them entered the end-stage of renal disease 3 years after onset. Conclusions For male pediatric patients with steroid resistant nephrotic syndrome, combined with non-renal manifestations such as multiple hernia or testicular abnormalities, the possibility of the hereditary nephrotic syndrome caused by NPHS2 mutations should be considered.

Sleep is a vital phenomenon of life,the disturbance of which could be associated with a wide range of diseases,such as depression,anxiety,memory loss and hypertension. After years of efforts,pharmacological research of sleep in China has been in line with the pace of international sleep study,especially in the mechanisms of sleep. Our research is beginning to involved in several hot spots of study,such as the relationship between sleep disorders and their comorbidities(PTSD, depression,hypertension,diabetes and neurodegenerative diseases). Attention has also been paid to the research and discovery of novel hypnotic drugs. Despite the gap in sleep research between China and other developed countries,sleep study in China will definitely step into a gold period as well as solve sleep problems for more patients as soon as possible with the joint efforts of researchers and with the increasing attention to healthy sleep.

Objective To analyze the diagnostic approach on hepatolenticular degeneration combined with thin basement membrane nephropathy.Methods A girl presented with microscopic hematuria, liver dysfunction and hypocomplementemia was diagnosed with hepatolenticular degeneration combined with thin basement membrane nephropathy, her clinical data were summarized and analyzed retrospectively.Results A ten years old girl presented with microscopic hematuria and liver dysfunction for a year, dysarthria for a month, and combined with hypocomplementemia but without proteinuria. Renal biopsy showed thin basement membrane nephropathy. Ceruloplasmin was 23.10 mg/L and urinary copper concentration was 120μg, respectively, ocular slit lamp examination showed Kayser-Fleischer ring, cranial MRI showed preternatural signal in both basal and putamen nucleus, mutation analysis showed homozygous mutations in ATP7B and heterozygous mutation in COL4A3 gene,respectively.Conclussion Hepatolenticular degeneration should be suspected in those cases with persistence microscopic hematuria, liver dysfunction and hypocomplementemia.

BACKGROUND:Some Chinese herbal ingredients produce the analogical and pharmacological effects equivalent to growth factors, which can promote cel proliferation as wel as tissue and organ recovery and regeneration. Additional y, they possess low price and relative stable physicochemical property. Thus, the applications of traditional Chinese medicine in tissue engineering has attracted more attentions of researchers al over the world. OBJECTIVE:To review the application of the traditional Chinese medicine in stem cel proliferation and tissue engineering. METHODS:A computer-based retrieval of PubMed and CNKI databases was performed for articles concerning the application of traditional Chinese medicine in stem cel proliferation and tissue engineering published from 1978 to 2015. The keywords were“the traditional Chinese medicine, tissue engineering, application, stem cel , anti-inflammatory, cross linking, surface modification, osteoblast, blood vessel”in English and Chinese, respectively. RESULTS AND CONCLUSION:Natural medicine and the traditional Chinese medicine are expected to be applied in tissue engineering as a surrogate of growth factor and antibiotics, which exert anti-inflammatory, angiogenesis, surface modification, anticoagulation and cross-linking roles as wel as promote proliferation and differentiation of stem cel s in tissue engineering.

Eighty simple obesity patients were treated by electoacupuncturing Tianshu(ST 25), Shuidao(ST 28), Shuifen(CV 9), Guanyuan(CV 4), Daheng(SP 15), Huaroumen(ST 24),Daimai (GB 26), Quchi(LI 11), Hegu(LI 4), Liangqiu(ST 34) and Zusanli(ST 36). The results showed remarkable effect in 14 cases, middle effect in 38 cases, common effect in 23 cases and failure in 5 cases.

Objective:To screen clinical pharmacists’ intervention points in the medical order safety management according to the classification of irrational medical order, ranking of risk and causes analysis in order to provide ideas and reference for clinical pharma-cists. Methods:The prior interventions and post reviews were conducted on medical orders in an intensive care unit of the hospital from July to December in 2014. Results:A total of 224 medical records and 6583 medical orders were intervened, reviewed and audi-ted. Among the medical orders, 653 cases were irrational with a ratio up to 9. 92%. The 653 irrational medical orders were mainly classified with incompatibility, inappropriate dosage and irrelevant combinations, and mainly D level errors according to the risk rating. Most of the irrational medical orders were due to the lack of professional knowledge. Conclusion: Clinical pharmacists should partici-pate in medical order safety management including interfering drug compatibility, dosage and combination therapy, which can reduce or even avoid the error occurrence in medical orders and promote safe and reasonable medication through reasonable medication training and prior interventions.

Objective To study the effect of apolipoprotein E (ApoE) polymorphism on serum lipids and the relationship between apolipoprotein E polymorphism and coronary heart disease (CHD).Methods Polymerize chain reaction (PCR) and HhaI digestion were used for the detection of apolipoprotein E genotype on 71 patients with CHD and 69 control subjects.Results The frequencies of apolipoprotein E4 allele and E3/4 genotype were obviously increased in CHD group than in control group (P<0.01,P<0.05). Conclusion The apolipoprotein E polymorphism is related to the occurrence of coronary heart disease; apolipoprotein E4 allele is one of the most important hereditary factor for the occurrence of CHD.