Humans ; Traumatology

Objective To explore the difference of pathogenesis between chronic hepatitis B (CHB) and asymptomatic hepatitis B virus(HBV) carriers(ASCs) in children.Methods Subtracted libraries was constructed by suppression subtractive hybridization (SSH) from peripheral blood mononuclear cell (PBMC) of two groups, beta-2-microglobulin (?_2-MG) was screened as one of differentially expressed genes. Serum was collected from children with CHB and ASCs, the differential expression of ?_2-MG was confirmed by enzyme immunoassay.Results Subtractive libraries were constructed successfully, the differentially expression of ?_2-MG and lactroferrin were up-regulated in CHB children.And the differentially expression of ?_2-MG on the level of protein was confirmed.Conclusion The up-regulation of ?_2-MG in CHB children is involved in the pathogenesis mechanisms.

OBJECTIVETo verify the feasibility of setup error verification by observing patents' source-skin distance (SSD) for patients of head tumor.

METHODSFilms for 21 patients with head tumor were recorded using simulator (Varian Acuity 8.6), and comparison with reference digitally reconstructed radiograph (DRR) from Treatment plan system (TPS). The deviation of setup for 21 patients in the left-right, anterior-posterior and superior-inferior directions were measured by using 2D match, and SSD error was recorded when gantry angle was 0 degrees, 45 degrees, 315 degrees. Then setup error and corresponding SSD error were analyzed.

RESULTSThe systematic errors and random errors of 21 patients in the left-right, anterior-posterior and superior-inferior directions were (1.1 +/- 11.6) mm, (0.7 +/- 1.2) mm, (0.9 +/- 1.5) mm, and (1.51 +/- 3.1) mm, (1.05 +/- 3.3) mm, (1.60 +/- 2.3) mm. The systematic SSD errors and random SSD errors were (1.25 +/- 1.3) mm, (1.04 +/- 1.3) mm. (1.10 +/- 2.3) mm, and (2.03 +/- 1.7) mm, (2.81 +/- 2.3) mm, (2.33 +/- 3.0) mm for gantry angle was 0 degrees, 45 degrees, 315 degrees, respectively.

CONCLUSIONSIt is simple and feasible for setup error verification by observing patients' SSD and can be auxiliary to other verification means.

Algorithms ; Brachytherapy ; methods ; Feasibility Studies ; Head and Neck Neoplasms ; radiotherapy ; Humans ; Radiotherapy Dosage ; Radiotherapy Planning, Computer-Assisted

A laccase gene (lacD) from the basidiomycete Trametes sp. 420 was heterologously expressed in Pichia pastoris in two ways, resulting in two recombinant enzymes of rLacDx with native N-terminus and rLacDe with eight additional amino acid residues at N-terminus. The yields of rLacDx and rLacDe in shaken-flask cultures after an 18-day growth were 1.21 x 10(5) u/L and 7.38 x 10(4) u/L, respectively, as determined with 2,2'-azinobis(3-ethylbenzothia-zoline- 6-sulfonic acid) (ABTS) as substrate. The yield of rLacDx was further increased to 2.39 x 10(5) u/L under high-density fermentation while the production process was decreased to 7.5 days. In addition, rLacDx and rLacDe exhibited similar enzymatic characters in oxidizing substrate guaiacol, and were stable at 50 degrees C and at a pH range from 3 to 10. However, the specific activity of rLacDx (1761 u/mg) for ABTS was higher than that of rLacDe (1122 u/mg), and the apparent Km value of rLacDx (427 microM) was less than that of rLacDe (604 microM).
Cloning, Molecular ; Fermentation ; Isoenzymes ; biosynthesis ; genetics ; Laccase ; biosynthesis ; genetics ; Pichia ; genetics ; metabolism ; Recombinant Proteins ; biosynthesis ; genetics ; isolation & purification ; Trametes ; enzymology ; genetics

Thirteen of 4-anilinoquinazoline derivatives with imine groups at position 6 of quinazoline ring were synthesized and their antitumor activities were evaluated by MTT assay and Western blotting analysis. Among these compounds, 13a-131 were reported first time. The MTT assay was carried out on three human cancer cell lines (A549, HepG2 and SMMC7721) with EGFR highly expressed. Among the tested compounds, 13i and 13j exhibited notable inhibition potency and their IC50 values on three cell lines were equivalent to or less than those of gefitinib. Compound 14, without imine group substituted, displayed excellent inhibitor potency only on A549 cell line. Compounds 14 and 13j were chosen to perform Western blotting analysis on A549. The results showed that both of the compounds could inhibit the expression level of phosphorylated EGFR remarkably. It was concluded that the inhibitor potency of compound 14 was almost equivalent to that of gefitinib and the inhibitor potency of 13j was better than that of gefitinib.
Aniline Compounds ; pharmacology ; Antineoplastic Agents ; pharmacology ; Cell Line, Tumor ; Humans ; Inhibitory Concentration 50 ; Phosphorylation ; Quinazolines ; pharmacology ; Receptor, Epidermal Growth Factor ; antagonists & inhibitors

OBJECTIVETo investigate the influence of escharectomy during shock stage on systemic and intestinal immune function and its mechanism in scalded rats.

METHODSNinety-six Wistar rats were employed in the study of which 8 were used as normal control group. The donor skin from the trunk in twenty-four rats were preserved in liquid nitrogen. The other 64 rats were subjected to 30% full-thickness scalding, and they were randomly divided into A (n = 24, no treatment after scalding), B (n = 24) and C (n = 16) groups. Physiological saline was intraperitoneally injected (50 ml/kg) on the 24 post-scalding hours to the rats in the B and C groups. The rats in B group underwent escharectomy during shock stage, and the excision wounds were covered with the cryo-preserved alloskin. The rats in C group received the same treatment as in B group but at 72 post-scalding hours. The change in the proliferative ability of splenic lymphocytes, the plasma and intestinal tissue content of interleukin 2 (IL-2), the contents of sIgA in intestinal mucus, and the content of DAO in the intestinal tissue were observed on 2, 4 and 8 post burn days (PBD) in A and B groups and also on 4 and 8 PBD in C group, respectively.

RESULTSThe splenocytic proliferative ability, IL-2 level in the plasma and intestinal tissue, and the sIgA content in intestinal mucus in the rats in A, B and C groups were lower than that in control group at all time points (P < 0.05). The proliferative ability of splenic lymphocytes in B group on 4 and 8 PBD and in C group on 8 PBD respectively was similar to that in control group. Whereas the IL-2 content in plasma and in intestinal tissue was higher in B and C groups than that in A group (P < 0.01). The sIgA content in intestinal mucus in B group was twice of that in C group respectively [(3.51 +/- 2.14) mg/g vs (1.40 +/- 0.64) mg/g, (3.03 +/- 0.95) mg/g vs (1.52 +/- 1.26) mg/g (P < 0.05 or P < 0.01)] on 4 and 8 PBD. The DAO activity in the intestinal tissue in A group was lower than that in control and B group (P < 0.05) on 4 and 8 PBD.

CONCLUSIONEscharectomy during shock stage might be beneficial to the recovery of the systemic and intestinal immune functions in rats with scalding injury.

Animals ; Burns ; immunology ; surgery ; Immunoglobulin A, Secretory ; immunology ; Interleukin-2 ; immunology ; Intestines ; immunology ; Male ; Rats ; Rats, Wistar ; Shock, Traumatic ; immunology ; surgery ; Skin Transplantation ; immunology ; T-Lymphocytes ; immunology

OBJECTIVETo establish a molecular method for the authentication of Pinellia pedatisecta and its adulterants.

METHODDNA sequences of some species from P. tenore, Typhonium and Arisaema were downloaded from GenBank, the sequences were aligned using DNAMAN. Allele-specific primers for P. pedatisecta and P. tenore were designed according to their SNPs in rpl 20 sequence. The designed primers were used to amplify 10 samples of P. pedatisecta, P. ternata and T. flagelliforme.

RESULTA 351 bp band was amplified from P. pedatisecta but not form P. ternata and T. flagelliforme by primer Pprpl149F and Pprpl484R. A 630 bp band was amplified from P. ternate and P. pedatisecta but not from T. flagelliforme by primer Ptrpl94F and Ptrpl699R.

CONCLUSIONAS-PCR has the advantages of highly specific and good reproducibility, by which P. pedatisecta can be identified from part of its adulterants quickly. It is a potential method to be used in the molecular identification of other materia medica.

Alleles ; China ; Consumer Product Safety ; DNA Primers ; genetics ; Pinellia ; genetics ; Plants, Medicinal ; genetics ; Polymerase Chain Reaction ; methods ; Polymorphism, Single Nucleotide ; Quality Control

OBJECTIVETo validate the therapeutic efficacy of paroxetine in the treatment of premature ejaculation (PE).

METHODSEighty PE patients up to the inclusion criteria were equally randomized to an experimental and a control group. We observed all the patients for 4 weeks and recorded the baseline data on intravaginal ejaculatory latency time (IELT) and sexual satisfaction scores, followed by oral medication of paroxetine at 20 mg/d for the patients in the experimental group and placebo for the controls. Thirty days after the treatment, we again recorded IELT and sexual satisfaction scores of the patients.

RESULTSAfter the treatment, the experimental group showed significantly prolonged IELT ([5.75 +/- 1.24] min) and increased sexual satisfaction score (6.4 +/- 1.2) as compared with the baseline data ([0.89 +/- 0.21] min and [2.7 +/- 0.9]) (P < 0.01). The control group exhibited no significant differences before and after the medication either in the mean IELT or in sexual satisfaction scores ([1.06 +/- 0.28] min vs [0.97 +/- 0.18] min and 3.6 +/- 1.3 vs 3.1 +/- 1.1, P > 0.05).

CONCLUSIONOral medication of paroxetine at 20 mg/d for 30 days could improve IELT and sexual satisfaction in PE patients.

Adult ; Ejaculation ; Humans ; Male ; Paroxetine ; therapeutic use ; Serotonin Uptake Inhibitors ; therapeutic use ; Sexual Dysfunction, Physiological ; drug therapy ; Treatment Outcome ; Young Adult

OBJECTIVETo apply pulse magnetic acupuncture at scalp acupoints to treat acute cerebral infarction and to explore the mechanism.

METHODSA pulse magnetic acupuncture group, a routine acupuncture group and a static magnetic acupuncture group were set up, 30 cases in each group. Their clinical therapeutic effects were observed.

RESULTSThe cured-markedly effective rate was 80.0% in the pulse magnetic acupuncture group and 70.3% in the routine acupuncture group with no significant difference between the two groups (P>0.05), which were significant difference with 36.6% in the static magnetic acupuncture group (P<0.01).

CONCLUSIONPulse magnetic acupuncture and routine acupuncture at scalp acupoints have same therapeutic effect on acute cerebral infarction, which is superior to that of static magnetic acupuncture.

Acupuncture Points ; Acupuncture Therapy ; Cerebral Infarction ; therapy ; Humans ; Scalp ; Stroke ; therapy

OBJECTIVETo develop a convenient and effective method for the identification of Bungarus multicinctus.

METHODBased on the sequence of Cyt b gene fragment of B. multicinctus and its adulterants, a pair of highly specific primer (HJL- and HJH-) were designed for distinguishing B. ulticinctus from other species of snake. To establish specific PCR reaction condition, the primers were employed to amplify the DNA templates extracted from B. multicinctus and 6 other species of snake, under different annealing temperature. Using this method, B. multicinctus was identified from 18 samples bought from many drugstores.

RESULTA 230 bp DNA fragment was amplified from B. multicinctus in PCR with annealed temperature at 67 degrees C, whereas no DNA fragment was amplified from other snake samples under the same reaction condition, B. multicinctus could be clearly distinguished from others by PCR reaction with the highly specific primers. In the present study, 18 sample, bought from different drugstores, were also identified by the highly specific PCR with the primers. The results indicated that 14 samples were B. multicinctus and the other 4 were adulterant, which was consistent with the conclusion of authentication based on morphological.

CONCLUSIONThe primers designed in the present study were highly specific for B. multicinctus.

Animals ; Base Sequence ; Bungarus ; classification ; genetics ; Cytochromes b ; genetics ; DNA ; genetics ; DNA Primers ; Drug Contamination ; Materia Medica ; Molecular Sequence Data ; Polymerase Chain Reaction ; methods ; Sequence Analysis, DNA ; Snakes ; classification ; genetics ; Species Specificity