Biomedical Research ; China ; Freeze Drying ; Hepatitis A Vaccines ; adverse effects ; immunology ; Humans ; Safety ; Vaccines, Attenuated ; adverse effects ; immunology

Objective To clinically observe and verificate the advantages of treating infantile anorexia of spleen-dysfunction in transportation with infantile massage and Chinese medicine based on the thought of “invigo-rating spleen and nourishing qi”. Methods Multi-central large-sample randomized controlled trial was carried out in Guangzhou, Shenzhen, and Foshan. Five hundreds cases of infantile anorexia of spleen-dysfunction in transportation were randomized into infantile massage group, Chinese medicine group, infantile massage combined with Chinese medicine group and Western medicine group and two sessions of treatment were required in each group. Separately, during the enrollment, after 2 sessions of treatment, infantile anorexia signs and symptoms scale , the related therapeutic effect criterion from clinical guideline of new drugs for traditional Chinese medicine, the expression level of hemoglobin and blood zinc were adopted for efficacy assessment. Results A mong 4 groups , the recovery rate and the total effective rate of infantile massage combined with Chinese medicine group were better than those of other groups (P < 0.05). The expression level of hemoglobin and blood zinc of 4 groups both increased obviously (all P < 0.01), but more improvement was found in infantile massage combined with Chinese medicine group (both P < 0.05). Conclusion The treatment of infantile anorexia with infantile massage and Chinese medicine based on the thought of invigorating spleen and nourishing qi has a significant effect.

Objective To compare the pharmacodynamics of common Bletilla striata powder(80 and 200 meshes) with ultra-fine powder of B.striata on experimental gastric ulcer in rats and to clarify how much the dose of B.striata was decreased after superfine comminution.Methods The models of gastric ulcer were set up by anhydrous alcohol and acetic acid to measure the gastric ulcer area and count out the inhibitory rate;The tissue slice of gastric ulcer cicatrix was got far HE staining.Based on the viewpoint of pathology the morbid state was observed.Results B.striata powder could decrease the gastric ulcer area significantly,and the groups of ultra-fine powder were advantaged to the others.Conclusion After superfine comminution,the potency of B.striata can be improved with its dose reducing.It is good for mo-dernization of making dosage forms.

AIM:To make compound demethycantharidin(including Radix astragali and Rhizoma bletillae) into stomach remaining-floating sustained-release preparation. METHODS:Radix astragal and Rhizoma betillae pulverized to superfine powders were combined with hydrophilic gel to manufacture stomach remaining-floating sustained-release tablet(floating tablet). RESULTS:The tablet started to drift in 3 min in the artificial gastric juice and float lasted for more than 8 h. By isotopic tracering,the floating tablets given on an empty stomach were retarded for 2-3 h and on large meal for 4-5 h in combination with ordinary tablet only retarded for 1 h. A pharmacopoeia dissolution method was set up to determine the release. 50% of tablets were disintegration in 5 h,70% in 8 h. The t 1/2 was 5.1 h(2-4 h for ordinary tablets),and the MRT was 8.3 h(3.3 h for ordinary tablets),and the remaining-floating sustained-release tablets showed a relative bioavailability of 230.71%. CONCLUSION:Pretreatment of raw herbs from compound demethylcantharidin and selection of the excipients will help to produce the herb-containing floating tablet.

Analysis of neural stem cells' movements is one of the important parts in the fields of cellular and biological research. The main difficulty existing in cells' movement study is whether the cells tracking system can simultaneously track and analyze thousands of neural stem cells (NSCs) automatically. We present a novel cells' tracking algorithm which is based on segmentation and data association in this paper, aiming to improve the tracking accuracy further in high density NSCs' image. Firstly, we adopted different methods of segmentation base on the characteristics of the two cell image sequences in our experiment. Then we formed a data association and constituted a coefficient matrix by all cells between two adjacent frames according to topological constraints. Finally we applied The Hungarian algorithm to implement inter-cells matching optimally. Cells' tracking can be achieved according to this model from the second frame to the last one in a sequence. Experimental results showed that this approaching method has higher accuracy compared with that using the topological constraints tracking alone. The final tracking accuracies of average of sequence I and sequence II have been improved 10.17% and 4%, respectively.
Algorithms ; Animals ; Cell Count ; Cell Movement ; Cell Tracking ; statistics & numerical data ; Image Processing, Computer-Assisted ; methods ; Microscopy, Fluorescence ; Models, Theoretical ; Neural Stem Cells ; cytology

To study the cleavage of neuron stem cells in time lapse image sequences and realize their features abstraction, identification and tracking, a precise segmentation algorithm that can preserve the shape of division cells is presented in this paper. The fuzzy threshold segmentation is based on Zadth's maximum entropy. The optimal parameters of the maximum fuzzy entropy are decided by genetic algorithm. Region merging and splitting of the under-segmentation objects of the result of fuzzy segmentation are realized by weighted distance transform, region labeling and some operations on morphology. By comparison with some results of fuzzy and hard segmentation, this algorithm can implement the precise segmentation that is necessary for some specified objects in automatic identification and tracking of neuron stem cells.
Algorithms ; Cell Culture Techniques ; methods ; Cell Division ; Computer Simulation ; Entropy ; Fuzzy Logic ; Humans ; Image Processing, Computer-Assisted ; Microscopy, Video ; methods ; Models, Biological ; Neurons ; cytology ; Stem Cells ; cytology

Adult ; Diagnosis, Differential ; Follow-Up Studies ; Humans ; Knee Joint ; Male ; Mucin-1 ; metabolism ; Neurilemmoma ; metabolism ; pathology ; surgery ; S100 Proteins ; metabolism ; Sarcoma, Synovial ; metabolism ; pathology ; Sweat Glands

Objective To establish a rabbit model of restenosis and analyze the expressions of VEGFmRNA and TGF-?_1mRNA during the intimal proliferation.We also explored the relationship between VEGFmRNA,TGF-?_1mRNA and restenosis.Methods 40 healthy male New Zealand white rabbits were evenly divided into three injury groups and one control group.Right carotid arteries were injured with PCI balloon in the injury groups.10 rabbits of each injury group were sacrificed on weeks 1,2 and 4 after the injury.VEGFmRNA and TGF-?_1mRNA were examined by in situ hybridization.All the samples were analyzed using a computerized imaging analysis system.Results In the injury groups,neointimal areas were significantly larger than those in control group(P

Compound Wuzhigan capsules is a compound preparation composed of Wuzhigan, Shidagonglao, Gangmei, Shanzhima. A Randomized, double-blind, multi-center, positive parallel control designed to evaluate the clinical efficacy and safety of compound Wuzhigan capsules on anemopyretic cold. One hundred and twenty anemopyretic cold patients were given compound Wuzhigan capsules (test group), 2 capsules one time, three times a day, 119 patients were given compound Wuzhigan tablets (control group) ,4 tablets one time, three times a day; three days of treatment The study showed, the markedly effective rate and total effective rate respectively were 63. 3% and 80% of the test group. For the control group, the markedly effective rate and total effective rate respectively were 72. 5% and 80. 7%. The difference was not statistically significant. Compound Wuzhigan capsules can reduce the dosage, and get better patient compliance.
Adult ; Capsules ; Common Cold ; complications ; drug therapy ; Double-Blind Method ; Drugs, Chinese Herbal ; adverse effects ; therapeutic use ; Female ; Humans ; Male ; Middle Aged ; Safety ; Treatment Outcome ; Young Adult

BACKGROUNDSingle fiber electromyography (SFEMG) is a sensitive technique for detecting abnormalities in neuromuscular transmission and is mainly used in the diagnosis of neuromuscular junction disorders, such as myasthenia gravis. While the process of denervation-reinnervation in amyotrophic lateral sclerosis (ALS) can also result in immature collateral nerve terminals and instability of neuromuscular transmission, the purpose of this study was to investigate the changes and clinical values of SFEMG in patients with ALS.

METHODSVolitional SFEMG was performed on the extensor digitorum communis (EDC) of 78 patients with ALS (men 52, women 26) who had been previously diagnosed by history, clinical features, and neurophysiological studies. The mean jitter, the percentage of jitter >55 micros, the impulse blocking percentage, and fiber density (FD) were determined. These results were compared to normal controls. In addition, the SFEMG indices were analyzed for correlations with the duration of ALS, the EDC strength score on the Medical Research Council (MRC) scale, and spontaneous activity detected by EMG studies.

RESULTSSFEMG indices were abnormal in all patients with ALS. Mean jitter ranged from 30 to 178 micros (mean 80.2 micros); the percentage of jitter >55 micros ranged from 5% to 100% (mean 60.5%). In addition, the impulse blocking percentage ranged from 0% to 90% (mean 28.1%) and FD ranged from 1.4 to 4.1 (mean 2.6). Mean jitter, the percentage of jitter >55 micros, and the blocking percentage in 57 patients with definite or probable ALS were significantly higher than in patients with possible or suspected ALS. MRC scores of the EDC negatively correlated with mean jitter, the percentage of jitter >55 micros, blocking percentage, and FD.

CONCLUSIONSSFEMG is the most sensitive tool for diagnosing definite or probable ALS. Increased jitter, blocking percentage, and FD can indicate the degree of immature collateral sprouts and motor end plates resulting from the progressive denervation and reinnervation associated with ALS, and may be helpful in evaluating prognosis.

Adolescent ; Adult ; Aged ; Amyotrophic Lateral Sclerosis ; physiopathology ; Electromyography ; Female ; Humans ; Male ; Middle Aged ; Muscle Fibers, Skeletal ; physiology ; Neural Conduction