1.Clinical Application of Portable Auto-haemostat Machine on Limbs
Mingxian GUO ; Wei LU ; Yan CUI
Chinese Medical Equipment Journal 1989;0(04):-
Objective To prove the clinical effect of portable auto-haemostat machine on body's limbs. Methods Portable auto-haemostat machine was applied to the indicated patients in their clinical aid treatments course, i.e. the rapid hemostasis on limbs. Results The hemostasis was rapid and accurate with hemostatic pressure in the range from 190 to 230 mmHg. The excellent effect guarantees the diagnosis, observation, bandaging and secure transmission of victims until they were sent to the operation room. Conclusion It is reliable to use portable auto-haemostat machine on body's limbs in clinic works.
2.Chemical constituents in rhizome of Pterocypsela elata and activity of lactuside B against brain ischemia
Heqin ZHAN ; Lanqing GUO ; Jianmin CUI ; Fulin YAN
Chinese Traditional and Herbal Drugs 1994;0(05):-
Objective To investigate the major chemical composition in rhizome of Pterocypsela elata and explore the activity of lactuside B against brain ischemia.Methods The compounds were isolated and purified by silica gel column chromatography and elucidated by spectroscopic experiments;The model of partial brain ischemia was used to detect water content,MDA and SOD levels in brain tissue in order to observe the activity of lactuside B against brain ischemia.Results Ten compounds were obtained and established as lactuside B(1),11?,13-dihydrolactucin acetate(2),?-sitosterol(3),daucosterol(4),(24R)-5?-stigrnast-7,22(E)-dien-3?-ol(5),3,3',4-trimethoxylellagic acid(6),?-amyrin(7),oleanolic acid(8),n-hexacosanic acid(9),and stearic acid(10).Lactuside B was a key component,and it's yield was 0.15%.Contents of water and MDA level in the brain tissue were significantly decreased,and the SOD content notably increased in all groups of lactuside B.Conclusion Ten compounds are all isolated from this plant for the first time.Compound 1 is a key component which possesses obvious activity against brain ischemia.
3.Study on Inheritance Methods of Academic Experiences of Famous Veteran Doctor of TCM Professor Huang Yongsheng
Jiajuan GUO ; Yingzi CUI ; Yan WEI ; Yongsheng HUANG
World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(5):1047-1050
The famous veteran doctors of traditional Chinese medicine ( TCM ) are models to combine TCM the-ories , previous practitioners' clinical experiences with current medical practice for the difficult to solve clinical problems . The selection of appropriate inheritance study method is especially important in the learning of fa-mous veteran doctors of TCM . This article discussed the inheritance method of academic experiences of famous veteran doctor of TCM professor Huang Yongsheng from four aspects, which were the reading of classic books, clinical practice, following medical masters, and summarization of clinical cases. It was hoped that during the inheritance process , efforts can be made to academic characteristics of TCM itself in accordance with its own law in order to create more and better clinical experience inheritance method.
4.Thought and Method of Clinical Therapeutic Evaluation System Constructing of Traditional Chinese Medicine in Coronary Heart Disease
Jiajuan GUO ; Yingzi CUI ; Yan WEI ; Yue DENG
World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(5):1037-1040
The overall concept is the core theory of traditional Chinese medicine ( TCM ) diagnosis and treat-ment system. During the process of building TCM effect evaluation on coronary heart disease (CHD), with the defined connotation and extension of heart diseases , except the consideration on its clinical symptoms , TCM four diagnosis and various current modern cardiovascular disease diagnostic methods should be organically com-bined according to the original macro basis . The micro quantitative evaluation is given on therapeutic effect of disease treatment in order to construct the complete TCM effect evaluation system.
5.The correlation analysis of the heart rate variability and executive function in patients with insomnia
Yan WANG ; Xianju GUO ; Xinghua HAO ; Jinghua WANG ; Yaohui CUI
Chinese Journal of Behavioral Medicine and Brain Science 2014;23(8):684-686
Objective To investigate status about the heart rate variability and executive function in patients with insomnia.Methods The study involves 165 cases of patients with insomnia and 92 cases normal.The Pittsburgh Sleep Quality Index (PSQI) screening for insomnia,Wisconsin Card Sorting Test (WCST) for the assessment of executive function and Self-generate Physiological Coherence System (SPCS) were used to collect the data of heart rate variability (HRV).Results The SDNN,RMSSD,TP,LF,HF of HRV of the insomnia group were significantly higher than these factors of control group (t=2.712,2.726,2.427,2.542,2.338,P<0.05 or P< 0.01).In the WCST,number of completed categories,conceptualization percentage of the group of patients with insomnia were significantly lower than the control group (t=-2.368,-2.165,all P<0.05),persistent errors,persistent error percentages,continuous number of responses of patients' group were significantly higher than the control group (t=2.712,2.991,2.690,all P<0.01).TP,LF,HF,LF/HF of HRV and the number of error responses,persistent errors,persistent error percentages,continuous number of responses of WCST had significant negative correlations (r=-0.171--0.245,P<0.05 or P<0.01),while the conceptualization percentage were significantly positively correlated (r=0.198-0.227,P<0.05 or P<0.01).Conclusion The results show that insomnia not only cause individual autonomic disorders,but damage the executive function of prefrontal.HRV and executive function in patients with insomnia have close relationships.
6.Difference between industrial hemp and marijuana hemp and industrial hemp's use value
Yanxu LU ; Peng DONG ; Xiaoguang CUI ; Jianshu GUO ; Yan WANG
Chinese Pharmacological Bulletin 2003;0(08):-
This paper reviewed the classification of hemp,distinction between industrial hemp and marijuana hemp,and the use value of industrial hemp.Some research problems and future research trends were also discussed.
7.Development of a portable automatic hemostatic instrument of extremities
Mingxian GUO ; Shipei CHEN ; Donghuan LIU ; Xijing JING ; Zhengkui GUO ; Yan CUI
Chinese Medical Equipment Journal 2003;0(12):-
The instrument is com posed of such components as SCM,pressure sensor,electric pump,magnetic valve and sleeve-type gasbag.It can control the hemostatic pressure,hemostatic time and slack time automatically.So the ischemic necrosis can be avoided thoroughly thr ough it.With the small volume,light weight,low power consumption and convenient operation,the instrument is fit for emergency treatment of mass casualties.
8.Influence of Human Chorionic Gonadotropin on Testicular Germ Cell Apoptosis of Experimental Unilateral Cryptorchidism in Rats
guo-fang, WANG ; cui-ping, SONG ; yan-fang, REN ; guo-lin, ZHANG
Journal of Applied Clinical Pediatrics 2006;0(17):-
Objective To explore the influence of human chorionic gonadotropin(HCG)on testicular germ cell apoptosis of experimental unilateral cryptorchidism in rats.Methods Forty immature male Sprague-Dawley rats were randomly divided into unilateral cryptorchi-dism group(n=20) and sham operation group(n=20).The 2 groups were divided into group treated with HCG and group without HCG.At age 21 days,unilateral cryptorchidism was produced.Half of the rats were injected with 20 U HCG from day 22 to 34 every other day.At age of 35 d and 60 d,rats were sacrificed for detection germ cell apoptosis by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL).Results Apoptosis index(AI) of cryptorchidism testis were higher compared with the scrotal testis in sham operation group(P0.05).AI of the scrotal testis of sham operation group and unilateral cryptorchidism group with HCG was higher compared with the correspond groups without HCG,and there were significant difference of AI between sham operation group and the correspond group without HCG at age 35 days(P0.05).Conclusions AI of testicular is increased both in cryptorchidism testis and scrotal testis in experimental unilateral cryptorchidism;HCG adds the number of apoptotic germ cells,and histology damage of testis is not completely recovery after stop using HCG.So clinical application of HCG must be cautious and operation ought to be done as early as possible in cryptorchidism.
9.Protective effect of astragalosides on anoxia/reoxygenation injury of hippocampal neuron.
Yan-Yan YIN ; Fen-Fang ZHU ; Guo-Cui WU
Chinese Journal of Integrated Traditional and Western Medicine 2010;30(11):1173-1177
OBJECTIVETo study the effect of astragalosides (AST) on the anoxia/reoxygenation (A/R) injured neuron in rat.
METHODSPrimary cultured rat's hippocampal neurons were made into A/R model cells. The cell viability was detected by MTT assay and lactate dehydrogenase releasing methods; the activity of superoxide dismutase (SOD), and contents of malondialdehyde (MDA) and nitride oxide (NO) in culture supernate were detected; the apoptosis rate of hippocampal neurons after A/R was measured by flow cytometry with double-staining of Hoechst33258 and AnnexinV-PI; and intracellular calcium ion [Ca2+]i was observed with a cofocal laser-scanning microscope and determined by fluorescent probe Fluo-3/AM.
RESULTSAST enhanced the cell viability of neurons after A/R injury, increased SOD activity and decreased the MDA and NO contents in supernate, reduced the A/R-induced apoptosis and decreased the calcium overload in neurons.
CONCLUSIONAST has the protective effects on A/R injured neurons, the mechanism is possibly related with its anti-oxidation and calcium overload reducing actions.
Animals ; Calcium ; metabolism ; Cell Hypoxia ; drug effects ; Female ; Fetus ; Hippocampus ; cytology ; Malondialdehyde ; metabolism ; Neurons ; cytology ; Neuroprotective Agents ; pharmacology ; Nitric Oxide ; metabolism ; Pregnancy ; Primary Cell Culture ; methods ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury ; prevention & control ; Saponins ; pharmacology ; Superoxide Dismutase ; metabolism ; Triterpenes ; pharmacology