1.A new eudesmane type sesquiterpene from cultivated Clerodendranthus spicatus in Hainan.
Hui-Qin CHEN ; Rong-Rong ZHANG ; Wen-Li MEI ; Cai-Hong CAI ; Cui-Juan GAI ; Xu-Dong YU ; Hao-Fu DAI
China Journal of Chinese Materia Medica 2019;44(1):95-99
Six compounds were isolated from the aerial part of cultivated Clerodendranthus spicatus in Hainan with various chromatographic techniques,and their structures were determined as:1-dehydroxy-1-oxo-rupestrinol(1),N-trans-feruloyltyramine(2),methyl 3,4-dihydroxyphenyllactate(3),caffein acid(4),methyl caffeate(5) and ethyl caffeate(6),via analysis of physicochemical properties and spectroscopic evidence.Compound 1 was a new compound,while compounds 2 and 3 were isolated from C.spicatus for the first time.Biological activity results showed that compounds 2-4 exhibited α-glucosidase inhibitory activity with different inhibition ratio.
China
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Glycoside Hydrolase Inhibitors
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isolation & purification
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pharmacology
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Lamiaceae
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chemistry
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Molecular Structure
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Phytochemicals
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isolation & purification
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pharmacology
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Sesquiterpenes, Eudesmane
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isolation & purification
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pharmacology
2.A new cytotoxic isoflavane from Dalbergiae Odoriferae Lignum.
Man-Man ZHU ; Hui WANG ; Cheng-Neng MI ; Wen-Li MEI ; Cui-Juan GAI ; Hao-Fu DAI ; Miao YU ; Xing-Quan WU ; Hao WANG
China Journal of Chinese Materia Medica 2020;45(9):2122-2129
Fourteen compounds were isolated from the ethanol extract of Dalbergiae Odoriferae Lignum by various chromatographic techniques, including column chromatographies on silica gel, Sephadex LH-20 and semi-preparative HPLC. Their structures were determined by spectroscopic techniques as S-3'-hydroxy-7,2',4'-trimethoxyisoxane(1), 2-(2',4'-dimethoxyphenyl)-6-hydroxybenzofuran(2), 2-(2'-hydroxy-4'-methoxyphenyl)-6-methoxybenzofuran(3), 7,2',4'-trimethoxydihydroisoflavone(4), sativanone(5), 3,9-dimethoxy-6H-benzofuro[3,2-c]chromen-6-one(6),(6 aS,11 aS)-homopterocarpin(7),(6 aS,11 aS)-8-hydroxy-3,9-dimethoxypterocarpan(8),(6 aS,11 aS)-3,8,9-trimethoxypterocarpan(9), isodalbergin(10), isoliquiritigenin(11), butein(12), butin(13) and 3,7,4'-trihydroxyflavone(14). Among them, compound 1 was a new compound, while 2 and 3 were new natural products, 6, 8, 9 and 14 were isolated for the first time from Dalbergiae Odoriferae Lignum. Compounds 1-14 were tested for their cytotoxic activity against human hepatoma cell line BEL-7402, human gastric cancer cell line SCG-7901, human lung cancer cell line A549, human chronic myeloid leukemia cell line K562 and HeLa human cervical cancer cellline by MTT method. Compound 1 exhibited significant cytotoxicity with IC_(50) values ranging from 2.85 to 11.62 μg·mL~(-1). In addition, 2, 11 and 12 showed weak cytotoxic activities.
Antineoplastic Agents
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Chromatography, High Pressure Liquid
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HeLa Cells
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Humans