Cough is a common respiratory symptom in children.To evaluate severity of cough and the quality-of-life in children with chronic cough,cough scores,generic questionnaires and cough-specific health-related quality of life questionnaires have been developed.Different questionnaires have their own particular features,recently the validity and reliability of the cough-specific health-related quality of life questionnaires are supposed to be high in assessing quality of life,and have been proved to be quite valid and reliable tools.Studies have shown that chronic cough not only affects physical status in children,but also seriously affects their psychological health,social activities and family life negatively.Therefore,evaluating severity of cough and impacting on quality of life contributes to the clinical development of appropriate interventions.

Integrated traditional Chinese and Western medicine therapy for pancreatitis has been used since the 1960s.According to the clinical manifestations and traditional Chinese medicine (TCM) syndrome differentiation of pancreatitis,pancreatitis was named true heart pain with cold limbs caused by spleen disease.Syndrome differentiation of acute pancreatitis and treatment with modified Dachaihu decoction achieved good clinical effects.After the 1990s,the research focus of pancreatitis research was shifted to severe acute pancreatitis (SAP).The clinical course of SAP was divided into three phases,and different therapeutic regimens were given.Clinical studies achieved good therapeutic effects in terms of cure rate and fatality rate.After 2000,integrated traditional Chinese and Western medicine therapy for chronic pancreatitis has been promoted systemically,and the cooperation between traditional Chinese medicine,endoscopic techniques,and operative treatment helps to significantly improve pain control,nutritional status,and incidence of complications.

Objective To probe the differences in clinicopathological features between benign and malignant polypoid lesions of the gallbladder.Methods In this study,1396 PLG cases diagnosed by postoperative pathology between 2007.1 to 2009.12 were enrolled.Cases were divided into three groups according to the pathological classification:1339 cases of benign proliferative diseases ;42 cases of adenoma,15 cases of malignant disease.Comparing the clinical characteristics of the three groups,we screened out the risk factors for malignant transitions.Results Age (F =8.090,P =0.000),size of polyp (F =102.61,P =0.000),single vs multiple lesions (x2 =214.25,P =0.000),concurrent inflammation (x2 =9.362,P =0.009),and stones (x2 =34.022,P =0.000) were significantly different between the three groups.Conclusions Size of polyps over 0.8 cm,age over 60 years,single polyp,accompanied by stones and inflammation were the risk factors for malignancy in gallbladder polypoid lesions.

Objective To study the effect of apolipoprotein E (ApoE) polymorphism on serum lipids and the relationship between apolipoprotein E polymorphism and coronary heart disease (CHD).Methods Polymerize chain reaction (PCR) and HhaI digestion were used for the detection of apolipoprotein E genotype on 71 patients with CHD and 69 control subjects.Results The frequencies of apolipoprotein E4 allele and E3/4 genotype were obviously increased in CHD group than in control group (P<0.01,P<0.05). Conclusion The apolipoprotein E polymorphism is related to the occurrence of coronary heart disease; apolipoprotein E4 allele is one of the most important hereditary factor for the occurrence of CHD.

Objective: To assess the effect of Linghuang powder ( LHP) in treating acute nonspecific lower urinary tract infection (ANLUTI) with l ower-Jiao damp-heat syndrome and evaluate its safety. Methods: A randomized cont rolled design was adopted. Four hundred and fourteen patients were allocated to LHP treatment group, control group and open treatment group. Results: The marked ly effective rate of short-term treatment with LHP was 71.78%, and the total effective rate was 92.02%, the total rates of im proving urine routine and urine bacterium were 95.21% and 79.37% respectively in LHP treatment group. Conclusion:The effect of LHP for ANLUTI with lower-jiao damp-heat syndrome is better than that in the control group (P<0.01).

Oxidative stress can achieve the purpose of tumor therapy by accelerating the death of tumor cells.As a major molecule generated from the body's oxidative stress reaction,reactive oxygen species(ROS)exerts the antitumor efforts by promoting apoptosis,necrosis and autophagy.The drugs which could increase the level of ROS in cells have received more and more people's attention,and this provide a new research direction for the clinical treatment of tumors.

In recent years,with increasing investment on laboratory medicine scientific research in China,the project management,implementation,evaluation system and quality improvement become more and more important for research managers and executives.In this paper,the main problems of project execution and implementation and accordingly proposed some constructive suggestions were analysed,in the aspects of the leading role of principal investigator,periodical discussion,peer guidance,scientific progress management,effective using of project funds and the establishment of appropriate reward and punishment mechanisms.It is hoped that these suggestions can be used to achieve success in laboratory medicine research project.

Objective To compare the effect of calcium sulfate pellets made by different methods in repair of segmental radial defect of rabbits. Methods Eighty white New Zealand rabbits were sub-jected to defects of middle part of the left radial bone and divided into four groups according to repair ma-terials: control group (Group A, implanted with no artificial bone substitute), uncoated pressed calcium sulfate pellets (Group B), coated pressed calcium sulfate pellets (Group C) and coated pressed calcium sulfate pellets combined with rhBMP-2 (Group D). Histologic examination and biological test were done at 4, 8 and 12 weeks after operation. The data were processed with mono-factor variance analysis. Re-sults New bone formation was found on the defected bone in Group D and Group C, with better in Group D. The bone strength test showed that the anti-bending strength was (39.6±1.7) % in Group C and (47.5±2.1) % in Group D, which were higher than (21.3±2.7) % in Group A and (23.6±3.3) % in Group B, with higher anti-bending strength in Group D than that in Group C (F = 125.3 ,P <0.01). Conclusions For restoration of segmental bone defects, chitosan-coated pressed calcium sulfate pellet shows relatively high density and slightly slow resorption, which closely coincides with the growth rate of new bone. The coated pellet combined with rhBMP-2 can enhance its osteogeneais in restoring segmental Done defects.

Objectives To explore the effects of gross hematuria on the results of several parmeters in laboratory urine examination. Methods Eighty (80) children with IgA nephropathy and 40 cases with acute post-streptococcal glomerulonephritis hospitalized in our hospital from January 2014 to December 2015 were recruited. The ratio of urinary calcium and protein to creatinine, quantitative test of 24 h urinary calcium and protein, quantitative test of 24 h urinary albumin,α1-microglobulinuria, microalbuminuria and urine protein electrophoresis were tested during and after the gross hematuria, respectively. Results The ratio of urinary calcium and protein to creatinine, quantitative test of 24 h urinary calcium and protein were much higher in the duration of gross hematuria as compared to those after the duration of gross hematuria, while α1-microglobulinuria, microalbuminuria and quantitative test of 24 h urinary albumin showed no difference between the two periods. Conclusions Gross hematuria could increase the level of urinary calcium and protein, while quantitative test of 24 h urinary albremin is not affected.

The normal value of parameters of saccade, smooth pursuit and optokinetic nystagmus has been detected by recording the electro-oculograph in 29 healthy subjects.The results are as follows: 1.The reaction time of saccade is 259.04? 74.19,the mean velocity is 216.83?87.51/s in 15? target amplitude.No statistical significance has been found between sexes, but the reaction time is obviously longer in pre-puberty group.2.For smooth pursuit in 40% target velocity, gain is 0.976?0.08, phase lag is 3.95?7.69s,moothness is 0.925?1.49.No statistical significance has been found between sexes.3.In analysing the sequential process of saccade and smooth pursuit, it can't observed the tendency of decrement in latency.4.The gain of OKN in 14?/s velocity is between 0.689-0.808, the gain decreases following the increase of target velocity.The symmetry of OKN is 3.54?15.04% and there are 81.58% of OKN belonging macula type in healthy people.5.When the target velocity is in close each other,the velocity of fast phase of OKN is in close with the velocity of saccade.