Cough is a common respiratory symptom in children.To evaluate severity of cough and the quality-of-life in children with chronic cough,cough scores,generic questionnaires and cough-specific health-related quality of life questionnaires have been developed.Different questionnaires have their own particular features,recently the validity and reliability of the cough-specific health-related quality of life questionnaires are supposed to be high in assessing quality of life,and have been proved to be quite valid and reliable tools.Studies have shown that chronic cough not only affects physical status in children,but also seriously affects their psychological health,social activities and family life negatively.Therefore,evaluating severity of cough and impacting on quality of life contributes to the clinical development of appropriate interventions.

Autoimmune disease is an own organization inflammatory lesions,mainly caused by destroying the adaptive immune tolerance mechanism of differentiatingself andnon-self,whose character is appearing the autoantibodies and self-reactive T cells in the body.Autoinflammatory disease is a group of genetic,recurrent and noninvasive inflammatory disease,whose characteristics are fever,rash,joint pain,arthritis,ophthalmic pathological changes and increasing of acute phase proteins,and it can affect many organ systems.These diseases are different in the mode of onset and clinical manifestations,but also can have similar and overlapped symptoms and signs,and often confused with other systemic diseases.Therefore,clinical misdiagnoses or missed diagnoses easily occur.To understand correctly and master the laboratory examination characteristics and its clinical is essential,which has significant value in the clinical diagnosis,differential diagnosis,evaluation and treatment of these diseases.

Primary aldosteronism (PA) is a common form of secondary endocrine hypertension,which is characterized by hypertension,hypokelamia,myathenia,elevated serum aldosterone concentration and suppressed plasma renin activity.Besides,accumulating research evidences showed that parathyroid hormone (PTH) level was elevated in patients with primary aldosteronism,accompanied by secondary hyperparathyroidism.This review systemically introduces the interaction between aldosterone and PTH in PA patients.

Eighty simple obesity patients were treated by electoacupuncturing Tianshu(ST 25), Shuidao(ST 28), Shuifen(CV 9), Guanyuan(CV 4), Daheng(SP 15), Huaroumen(ST 24),Daimai (GB 26), Quchi(LI 11), Hegu(LI 4), Liangqiu(ST 34) and Zusanli(ST 36). The results showed remarkable effect in 14 cases, middle effect in 38 cases, common effect in 23 cases and failure in 5 cases.

In recent years,with increasing investment on laboratory medicine scientific research in China,the project management,implementation,evaluation system and quality improvement become more and more important for research managers and executives.In this paper,the main problems of project execution and implementation and accordingly proposed some constructive suggestions were analysed,in the aspects of the leading role of principal investigator,periodical discussion,peer guidance,scientific progress management,effective using of project funds and the establishment of appropriate reward and punishment mechanisms.It is hoped that these suggestions can be used to achieve success in laboratory medicine research project.

Objective] To analyse the course of treatment based on differentiation of signs of Hashimoto thyroiditis combined with many diseases, offering treating thought and medication experiences. [Method] Trace back and state the relative documents about the general conditions, diagnosis and therapy, symptoms-pointed treatment of 1 case of Hashimoto thyroiditis combined with many diseases, introducing Pro. Wumin's clinical experience in treating the patient. [Result] The patient was early or late diagnosed as fatness, hyperlipemia, hyperinsulinemia, sub-clinical Hypothyrea, thyroid nodule and type-2 diabetes, Pro. Wumin combined TCM with WM, the patient was control ed the diseases and kept stable. [Conclusion] Combination of TCM and WM treating Hashimoto thyroiditis accompanied with diseases above has good cure effect, the patient is satisfied with the improvement and result.

Biomarkers are objective tools with an important role for diagnosis in patients with acute myocarditis.Traditional biomarkers,such as aspartate aminotransferase,creatine kinase and myoglobin seem to have low sensitivity.Creatine kinase-MB′s major limitation is short in half-life,which makes monitoring more difficult.To date,cardiac troponins are closest to optimal biomarker with high sensitivity and specificity in the early diagnosis of acute myocarditis.Novel biomarkers,including stromal cell-derived factor 1 ,heart-type fatty acid binding protein and MicroRNA have the potential to be valuable biomarkers for early diagno-sis.Combining biomarkers and clinical features have appeared to improve diagnostic accuracy than either one alone.

Oxidative stress can achieve the purpose of tumor therapy by accelerating the death of tumor cells.As a major molecule generated from the body's oxidative stress reaction,reactive oxygen species(ROS)exerts the antitumor efforts by promoting apoptosis,necrosis and autophagy.The drugs which could increase the level of ROS in cells have received more and more people's attention,and this provide a new research direction for the clinical treatment of tumors.

Objective To compare the effect of calcium sulfate pellets made by different methods in repair of segmental radial defect of rabbits. Methods Eighty white New Zealand rabbits were sub-jected to defects of middle part of the left radial bone and divided into four groups according to repair ma-terials: control group (Group A, implanted with no artificial bone substitute), uncoated pressed calcium sulfate pellets (Group B), coated pressed calcium sulfate pellets (Group C) and coated pressed calcium sulfate pellets combined with rhBMP-2 (Group D). Histologic examination and biological test were done at 4, 8 and 12 weeks after operation. The data were processed with mono-factor variance analysis. Re-sults New bone formation was found on the defected bone in Group D and Group C, with better in Group D. The bone strength test showed that the anti-bending strength was (39.6±1.7) % in Group C and (47.5±2.1) % in Group D, which were higher than (21.3±2.7) % in Group A and (23.6±3.3) % in Group B, with higher anti-bending strength in Group D than that in Group C (F = 125.3 ,P <0.01). Conclusions For restoration of segmental bone defects, chitosan-coated pressed calcium sulfate pellet shows relatively high density and slightly slow resorption, which closely coincides with the growth rate of new bone. The coated pellet combined with rhBMP-2 can enhance its osteogeneais in restoring segmental Done defects.

Objective To probe the differences in clinicopathological features between benign and malignant polypoid lesions of the gallbladder.Methods In this study,1396 PLG cases diagnosed by postoperative pathology between 2007.1 to 2009.12 were enrolled.Cases were divided into three groups according to the pathological classification:1339 cases of benign proliferative diseases ;42 cases of adenoma,15 cases of malignant disease.Comparing the clinical characteristics of the three groups,we screened out the risk factors for malignant transitions.Results Age (F =8.090,P =0.000),size of polyp (F =102.61,P =0.000),single vs multiple lesions (x2 =214.25,P =0.000),concurrent inflammation (x2 =9.362,P =0.009),and stones (x2 =34.022,P =0.000) were significantly different between the three groups.Conclusions Size of polyps over 0.8 cm,age over 60 years,single polyp,accompanied by stones and inflammation were the risk factors for malignancy in gallbladder polypoid lesions.