ObjectiveTo explore the influence of L-carnitine on the effect of erythropoietin in treatment of patients with renal anemia during hemodialysis treatment.Methods40 uremic patients with chronic renal failure underwent bemodialysis(HD) in were randomly divided into treatment group and control group,20 cases each.Control group was given EPO 9000u/week subcutaneous injection in the blood after dialysis,while regular oral iron,folic acid.Treatment group on the basis of the control group to 1.0g L-carnitine added 20ml 0.9％ sodium chloride injection intravenously 3 ～ 5 min,2 times per week.Both groups were treated for 3 months.Before treatment and after 4 weeks and 3 months treatment.The hemoglobin(Hb),hematocrit (Hct),serum ferritin (SF),serum thyroxine(iPHT) and compared.ResultsAfter 4 weeks of treatment,Hb,Hct levels increased compared with before treatment( all P ＜0.05).Hb after 3 months compared with the two groups before treatment were significantly higher(all P ＜0.05),and Hb increase of the treatment group significantly higher than the control group ( P ＜ 0.01).ConclusionL-carnitine could improve the treatment of erythropoietin in hemodialysis patients with renal anemia.

Objective To evaluate the clinical application value of amniotic membrane transplantation in the treatment of intrauterine adhesions.Methods Totally 48 intrauterine adhesions patients of infertility were treated with amniotic membrane transplantation from January to December 2013 in our hospital.They were divided into mild adhesion in 11 cases (group A),moderate adhesion in 17 cases (group B),and severe adhesive group in 20cases (group C) by American Fertility Association.They were treated with amniotic membrane transplantation and estrogen/progesterone sequential treatment after operation.Clinical effect and feasibility of each group were analyzed.Results There was statistical significance in the endometrium thickness after 3 months (P ＜ 0.05).The menstruation of all patients with mild and moderate intrauterine adhesion (IUA) was improved,and the menstrual improvement rate in patients with severe intrauterine adhesions was 90％.IUA cure rate of groups A,B,and C were different after 3 months of surgery,[groupA100％ (11/11),group B 94.1％ (16/17),group C70％ (14/20),x2 =6.73,P ＜0.01],The cure rate of group A was significantly higher than that of group C (x2 =4.00,P ＜ 0.05),although the cure rate of group B was higher than that of group A,but the difference was not statistically significant (P ＞ 0.05).Conclusions The cure rate of amniotic membrane transplantation combined with estrogen in the treatment of intrauterine adhesions was high.It could promote the recovery of the endometrium and improve the recovery of menstruation.There was no transplantation rejection and safety.It also had the advantages of easy drawing materials,simple operation,and can be applied to clinical application and promotion.

Objective To study the current status of morning blood pressure peak in elderly masked hypertension patients and study the correlation between morning blood pressure peak and cognitive function.Methods A total of 72 elderly masked hypertension patients were selected in this study,.Morning blood pressure peak was measured by ABPM examination.The patients were divided into three groups according to the morning blood pressure peak.Morning blood pressure peak ≤ 30 mmHg were divided into non-Morning blood pressure surge group ( NMS group),morning blood pressure peak ＞30 mmHg were divided into Morning blood pressure surge group ( MS group).The cognitive function scale was determined (MMSE and MoCA).T-test and linear regression analysis were performed.Results MMSE score(27.69±2.04) and MoCA scores(24.85 ± 1.52) in MS group were lower than NMS group (28.87 ± 1.34,26.54± 1.53) ( P ＜0.01 ).MMSE and MoCA scores were negatively correlated with the morning blood pressure peak ( r =- 0.308,- 0.447,P ＜ 0.01 ).Conclusions Elderly masked hypertension patients remained the phenomenon of the morning blood pressure peak,the morning blood pressure peak could lead to cognitive impairment.

Bronchoalveolar lavage is a lung interventional technique,it has a good prospect in the diagnosis and treatment of childhood respiratory diseases,especially in severe and difficult cases.This article focused on the role of bronchoalveolar lavage in the diagnosis and treatment of respiratory diseases in children,described the impact of pulmonary surfactant.

Objective　To study the alterations of surfactant protein A (SP-A) and B (SP-B) in rats in the early stage of acute lung injury induced by oleic acid. Methods　An acute lung injury model was established by injecting oleic acid into the superior vena cava of rats and the levels of SP-A and SP-B in branchoalveolar lavage fluid (BALF) were determined with Western Blot and ELISA. Results　The levels of SP-A and SP-B in BALF were significantly decreased in the injured group 1 h after acute lung injury (P<0.01) and remained low in the next 6 h. Conclusion　The levels of SP-A and SP-B were decreased in the early stage of acute lung injury. The methods used for detecting SP-A and SP-B we adopted are specific and sensitive, and might be helpful for other experimental study.

Objective To study the effect of apolipoprotein E (ApoE) polymorphism on serum lipids and the relationship between apolipoprotein E polymorphism and coronary heart disease (CHD).Methods Polymerize chain reaction (PCR) and HhaI digestion were used for the detection of apolipoprotein E genotype on 71 patients with CHD and 69 control subjects.Results The frequencies of apolipoprotein E4 allele and E3/4 genotype were obviously increased in CHD group than in control group (P<0.01,P<0.05). Conclusion The apolipoprotein E polymorphism is related to the occurrence of coronary heart disease; apolipoprotein E4 allele is one of the most important hereditary factor for the occurrence of CHD.

ObjectiveTo improve nursing quality by paying attention to management of nurses' psychology and behavior and changing individual and collective behavior.MethodsGuided by dynamic field theory,giving nursing staff continuous periodic training and counseling,establishing a staff interview system,giving systematic support and enhancement to develop a high-performance team.A case-control study was carried out in eight wards to evaluate implementation effect by comparison study.ResultsBefore and after introducing dynamic field theory,there were significant differences in job satisfaction of nurse staff (P<0.01),in patients' satisfaction(P<0.05),in evaluation of nursing staff management efficiency(P<0.05).ConclusionsApplication of dynamic field theory in nursing management can improve job satisfaction of nurse staff,patients' satisfaction and nurses' post suitability,and obtain continuous nursing quality improvement.

Objective To investigate the effects of angiotensin Ⅱ (Ang Ⅱ ) and angiotensin Ⅱ type 1 receptor antagonist (AT1RA) on the transforming growth factor-β1 (TGF-β1) synthesis and tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA expression of in vitro cultured hepatic stellate cells (HSCs). Methods HSC-T6 rat hepatic stellate cell line was selected as the study model of the activated hepatic stellate cells. Cultured HSCs were randomized into control group, Ang Ⅱ group, AT1RA group and Ang Ⅱ AT1RA group. Cell culture medium was used to detect the TGF-β1 level by ELISA method. HSCs were harvested to measure the TIMP-1 mRNA expression by RT-PCR. Results TGF-β1 level of control group, Ang Ⅱ group and AngⅡ AT1RA group in cell culture medium was (7.531 ±0. 654) pg/mL, (9. 855± 1. 485)pg/mL and (7.719 ± 0.329) pg/mL respectively, Ang Ⅱ group higher than control group (P ＜ 0.05 ), Ang Ⅱ AT1RA group lower than Ang Ⅱ group (P ＜ 0. 05 ). TIMP-1 mRNA expression level of control group, Ang Ⅱ group and Ang Ⅱ AT1RA group in HSCs was 3. 387 ± 0. 042, 4.870 ± 0.061 and 3. 837 ± 0. 042 respectively, Ang Ⅱ group higher than control group ( P ＜ 0. 05 ), Ang Ⅱ AT1RA group lower than Ang Ⅱ group (P ＜ 0. 05). Conclusion AngiotensinⅡ can increase the TGF-β1 synthesis and TIMP-1 mRNA expression of hepatic stellate cells, while all these effects are inhibited by angiotensinⅡ type 1 receptor antagonist.

After the concept of symptoms in traditional Chinese medicine was defined, the classification of symp-toms in traditional Chinese medicine and the characteristics of clinical diagnosis and treatment in traditional Chinese medicine were described .It was suggested that the symptoms in traditional Chinese medicine should be classified into 3 kinds, namely symptoms and signs according to the 4 diagnostic methods, symptoms and signs according to the diagnostic devices, symptoms and signs according to the physical constitutions of patients in order to lay a foun-dation for improving the classification in clinical term system of traditional Chinese medicine.

Objective\ To determine the distribution of the sympathetic postganglionic neurons innervating the uterus of uterine tube in hens. Methods\ CB\|HRP was injected into the wall of the uterus(shell gland)of hens. Results\ The hens uterus was innervated by the bilaterally sympathetic postganglionic neurons from T\-1 to LS\-\{13\} segments of the sympathetic trunk,splanchnic and adrenal ganglia.CB\|HRP labeled neurons in the left side were prominant.In the sympathetic trunk,a good lot of labeled neurons were accummulated in the segments both T\-5\|LS\-2 and LS\-8\|LS\-\{12\}. The greatest concentration of the neurons was in T\-7 and LS\-\{10\}. Conclusion\ The hen's uterus was innervated by the bilaterally sympathetic ganglionic neurons although it was the unilateral organ.\;