0.05).The prevalence rates of ID in first trimester group and second trimester group were 14.29% and 33.81%,respectively,showing significantly higher rate in the latter group(?2=6.33,P

Objective:To better understand the karyotype distribution ofthe patients with Down's syndrome(DS)who have been finally diagnosed in our hospital.To compare the strengths of G-banding karyotype analysis and FISH(Fluorescence In Situ Hybridization, FISH)in diagnosing the DS patients with the Chromosome mosaicism and Calculating the percentage of abnormal karyotypes.Methods:Blood of the patients who were likely to suffer from DS was selected for G-banding karyotype analysis to describe the karyotype distribution.5 of the finally diagnosed patients with the chromosome mosaicism were randomly selected for FISH to compare the percentage of abnormal karyotypes of the two methods.Results:287 DS patients were diagnosed,91.29%of whom had the simple karyotype.Among the patients with the translocation karyotype, 11 D/G translocation karyotype were detected,accounting for 73.33%,while 4 G/G translocation karyotype were diagnosed, constituting 26.67%.One patient with both of the translocation karyotype and the mosaicism karyotype was detected in our research,which was uncommon in recent report.The results of FISH and G-banding karyotype analysis were consistent.There was no significant difference of the abnormal karyotype rates for DS patients with the chromosome mosaicism by G-banding karyotype analysis and FISH.Conclusion:The simple karyotype is the main karyotype of the DS patients in our research.Among the translocation karyotype,the D/G kind is common.DS patients can be diagnosed efficiently by G-banding karyotype analysis,but we still suggest to use FISH to get the proportion of the abnormal cell for the patients who have less triploid cell and slighter clinical manifestation.

With the development of antibody manufacturing technology and improvement of new drug research in domestic industry, more innovative monoclonal antibody products submitted investigational new drug (IND) application. At the same time, monoclonal antibody products from abroad which have been approved marketing authorization and/or conducted clinical trials submitted IND applications in China. The National Medical Products Administration (NMPA) issued the "Guideline of Investigational New Drug Application" (No. 16, 2018) which emphasized the chemical, manufacturing, and control (CMC) regulatory, and dossier requirements in IND application, greatly promoted the application quality of innovative biological products. However, compared to the Food and Drug Administration (FDA) and European Medicines Agency (EMA), our particular guidelines are insufficient, such as guideline on virus safety evaluation of biotechnological investigational medicinal products. This review investigated the questions raised by sponsors from 2018 to 2020, including the end of production cell (EOPC) and/or unprocessed bulk (UPB) testing and virus removal or inactivation validation. Meanwhile, sponsors submitted different dossiers due to differences in understanding of stage requirements of guidelines from domestic and abroad. Based on the guidelines of virus safety from NMPA, FDA, and EMA, and the technical considerations, this review puts forward personal suggestions on the adventitious agents testing and virus removal or inactivation validation in manufacturing process, aim to ensure virus safety of innovative monoclonal antibody products in clinical trials.

Objecfive To study T cell subsets distribution in peripheral blood from patients with ankylosing spondylitis(AS)and the role of cell immunity in AS.Methods 30 patients with untreated active phase AS and 30 healthy volunteers were enrolled.The expression of CD4+ and CD8+ T cell subsets were evaluated by flow eytome try.The correlation among T cell subsets and Bath AS function index(BASFI).Bath AS measurement index(BASMI),course of disease,age,ESR,hyper-sensitive C-reactive protein (hs-CRP) were analyzed.Results The level of CD4+ T cell and CD4/CD8+ratio were significantly lower than that of healthy volunteers[(29.24±9.22)% vs.(40.09±6.86) %,(0.96±0.49 ) vs.(1.70±0.67 ),P ＜ 0.01 ],and CD8+ T cell were significantly higher than that of healthy volunteers [(32.91±6.86) % vs.(25.60± 5.97 ) %,P ＜ 0.01 ].The level of CD4+ T cell subsets in peripheral blood of AS patients was negatively correlated with BASMI (r =-0.479,P ＜ 0.01 ),and CD8+ T cell subsets were positively correlated with ESR,hs-CRP,BASFI and BASMI ( r = 0.373,0.430,0.462,0.530,P ＜0.05 ).The ratio of CD4+/CD8+ was negatively correlated with hs-CRP,BASFI and BASMI (r = -0.465,- 0.473,- 0.426,P ＜ 0.05 ).CD4+,CD8+,ratio of CD4/CD8 were not significantly correlated with age and course of disease in patients with AS.Conclusion T cell subsets are significantly abnormal in peripheral blood of patients with AS,and the imbalance degree of T cell is correlated with severity and activity of AS,suggesting that T cell subsets imbalance plays an important role in the pathologensis of AS.

Objective To evaluate the removal effectiveness of air pollutants by photocatalytic nanocomposite coating. Methods TiO2-coated glass board was exposed to UV light of 365 nm in chamber with volume of 4 m3. The purification efficiencies of TiO2 nanocomposite coating for SO2?NO2 and formaldehyde were determined at light intensity of 107-271 ?W/cm2 and wind velocity of 0.3-1.5 m/s. Results The purification efficiencies of TiO2-coated glass board treated under this experimental condition for 30 min were 97%?90%, 69% for SO2?NO2 and formaldehyde in indoor air respectively, and only 48% for SO2 without exposure to UV light. Conclusion This method provided basic data for establishing an evaluation method for purification effectiveness of photocatalytic nanocomposite coating for indoor air pollutants. Further approach was needed for exploring the effects of various environmental influential factors on the purification effectiveness of TiO2 nanocomposite coating.

Hepatocellular carcinoma(HCC) is one of the most common malignant tumors with the highest cause of death and increasing incidence worldwide, and the annual incidence rate is rising. The early diagnosis of HCC is very essential to its prognosis. At present, AFP has been widely used in the survey of HCC diagnosis, therapeutic effect and predict recurrence. However, the sensitivity and specificity of AFP is not satisfactory. In recent years a variety of new serum tumor markers have emerged one after another. A new serum marker-Golgi glycoprotein-73(GP-73) in early diagnosis of HCC is expected to become the new target maker,its sensitivity and specificity are better than AFP.

In order to optimize the curriculum structure, improve the quality of the traditional med-ical curriculum which separates the theory teaching and clinical teaching in medical education; give full play to the advantages of the multiple disciplines application to guide students to establish a holistic view of medicine; cultivate students'! independent thinking and team cooperation ability, we explore the reform model of the pediatric teaching courses from the following five aspects:curriculum design, class hour arran-gement, teaching team building, teaching carrier production and application of problem-based learning (PBL) teaching method. Preliminarily establish the teaching mode in multi disciplines integration which is characterized by taking a certain symptom or sign as leading clue.

Objective To investigate the effects of rutaecarpine on the cerebral injury and calcitonin gene-related peptide (CGRP) level during focal cerebral ischemia-reperfusion injury in rats. Methods Rats were intravenously given three doses of rutaecarpine (50, 100 and 300?g/kg) or vehicle 30 minutes before experiment. Then the rats subjected middle cerebral artery occlusion (MCAO) for 2 hours, and neurological deficits scores were performed at 6, 12 and 24 hours after reperfusion. After the last test the animals were sacrificed, the infarct volumes were determined by TTC staining, and CGRP levels were measured by radioimmuoassay. Results Rutaecarpine significantly reduced infarct volume, and improved cerebral function in dose-dependent manner compared with vehicle. Rutaecarpine significantly inecreased brain CGRP levels after reperfusion as well. Conclusion Rutaecarpine has marked protective effects on ischemic brain damage in rats possibly by increasing CGRP release in the brain.

Objective To investigate the frequencies of variant of lipoprotein lipase(LPL)gene,HindIII site at intron 8 and PvuII site at intron 6,and its relation with type 2 diabetes mellitus and insulin resistance(IR).Methods PCR-RFLP method was used to determine the DNA polymorphism of 8 th intron and 6 th intron of LPL gene in 78 T2DM patients and 49 controls.Results(1)Compared with control group,the frequencies of H+H+ genotypes and alleles ″H+″ was significantly higher in type 2 diabetes patients(P

Sudden cardiac death (SC D ),m ostcom m only seen in coronary heart disease, is a kind of sud-den death caused by series of cardiac param eters, w hich usually com bines w ith m yocardial infarction. H ow ever, som e SC D s (including early m yocardial infarction) happen suddenly and cause death in a very short tim e. In these circum stances, typical m orphological changes are lack in m acroscopic or m icroscopic fields, w hich m ake such SC D s becom e the em phasis and difficulty in the present research. SC D caused by m yocardial infarction and abnorm alities of cardiac conduction system (C C S ) is related to atheroscle-rosis of coronary artery closely. T his paper review s cardiac dysfunction caused by m yocardial infarction and diseases of C C S from m orphology and m olecular biology, and explores potential relationship be-tw een them . T his paper aim s to provide clues to the m echanism of m yocardial infarction related sudden death and possible assistance for forensic diagnosis of SC D .