Antigens, Bacterial ; Humans ; Inflammation ; Nasal Mucosa ; immunology ; Staphylococcus ; immunology ; Superantigens ; immunology

Child, Preschool ; Cysts ; Diagnostic Errors ; Humans ; Leiomyoma ; diagnosis ; Male ; Nose Neoplasms ; diagnosis ; Vestibular Diseases

Adult ; Female ; Humans ; Meningeal Neoplasms ; complications ; diagnosis ; Meningioma ; complications ; diagnosis ; Otitis Media with Effusion ; diagnosis ; etiology ; Skull Base Neoplasms ; complications ; diagnosis

Objective To evaluate the effect and feasibility of coblation treatment for laryngeal papilloma in adult. Methods A total of 18 patients with laryngeal papilloma treated by coblation from April 2008 to June 2010 was retrospectively analysed. There were 4 cases of multiple laryngeal papilloma and 14 cases of single laryngeal papilloma. All of these patients were treated with CoblatorTM Reflex #7070 under general anesthesia without tracheotomy. Results The volumes of blood loss during surgery varied from l ml to 10 ml, 2 ml on average. There was no postoperative bleeding or other complications. Follow-ups ranged from 6 months to 33 months after surgery, median 18 months. Recurrence occurred 4 months and 8 months after surgery in two patients. They were treated with coblation for the second time and followed up for 4 months and 12 months respectively. Recurrence occurred again in one patient. Conclusion Coblation under suspension laryngoscope for laryngeal papilloma in adult was an ideal way due to less bleeding and less damage.

To explore the new method of discriminating Astragali Radix and Hedysari Radix by using PCR amplification of specific alleles, 30 samples of the different Astragali Radix materials and 28 samples of Hedysari Radix were collected. The total DNA of all samples were extracted, trnL-trnF sequence from Astragali Radix and Hedysari Radix was amplified by PCR and sequenced unidirectionally. These sequences were aligned by using Clustul W. Primer was designed and the PCR reaction systems including annealing temperature, dNTP, etc were optimized. All samples were amplified by PCR with specific primer, DNA from Astragali Radix would be amplified 136 bp, whereas PCR products from all of Hedysari Radix were 323 bp. This method can detect 10% of intentional Hedysari Radix DNA into Astragali Radix. PCR amplification of alleles can be used to identify Astragali Radix and Hedysari Radix successfully and is an efficient molecular marker for authentication of Astragali Radix and Hedysari Radix.
Alleles ; Astragalus Plant ; classification ; genetics ; DNA Barcoding, Taxonomic ; DNA, Plant ; genetics ; Polymerase Chain Reaction

OBJECTIVETo evaluate the relationship between the expression of monocyte chemotactic protein 1 (MCP-1) and vascular endothelial growth factor (VEGF) in nasal polyp (NP) tissues and the role of MCP-1 in the formation of nasal polyps.

METHODSForty nasal polyp tissues obtained from NP patients were used to detect the expression of MCP-1 and VEGF at the levels of protein and mRNA by immunohistochemical and in situ hybridization assay. Twenty-five inferior turbinate (IT) samples obtained from chronic rhinitis patients were used as control.

RESULTSThe expression of MCP-1 and VEGF was higher in NP tissue than in IT at protein and mRNA levels (P <0.01). The expression of these two cytokines was positively correlated(r = 0.871, P <0.05).

CONCLUSIONSMCP-1 and VEGF were involved in the formation of nasal polyps.

Adult ; Aged ; Chemokine CCL2 ; metabolism ; Female ; Humans ; Male ; Middle Aged ; Nasal Mucosa ; metabolism ; Nasal Polyps ; metabolism ; pathology ; Vascular Endothelial Growth Factor A ; metabolism

Objective To evaluate the effect of endoscopic surgery using the low-temperature plasma radiofrequency for nasal hemangioma.Methods Clinical data of 15 patients treated between October 2007 and Ocwber 2009 under endoscopic surgery using the low-temperature plasma radiofrequency were retrospectively studied.Results All tumors in 15 patients were completely removed.The blood loss was 1-150 ml and the average blood loss was about 15 ml,only gelatin sponge was used to protect the wound after operation.There was no additional packing to stop bleeding.No complications were seen.The patients had mild postoperative pain.All patients were followed-up from 2 months to 2 years,no recurrence was found.Conclusions Endoscopic surgery using low-temperature plasma radiofrequency for nasal hemangioma has following advantages such as simplicity,minimal invasion and so on.It is a viable surgical method for the treatment of nasal hemangioma.

Objective To evaluate the feasibility of endoscopic surgery using the low-temperature plasma radiofrequency for nasopharyngeal angiofibroma (NA). Methods The clinical data of 4 patients treated between December 2005 and August 2009 were retrospectively analyzed. The tumor in one patient was at stage Ⅰ and three patients were at stage Ⅱ, all these patients were treated by endoscopic surgery using the low-temperature plasma radiofrequency under controlled hypotension anesthesia. Preoperative angiography and embolisation were underwent in all patients. Bipolar coagulation was used to help hemostasis during operation. Results The tumor in all 4 patients was completely removed. The operation time was 60,80, 110, 90 min respectively. The blood loss was 250, 250, 320, 280 ml respectively. Neither blood transfusion nor complications were encountered perioperatively. Follow-up ranged from 6 months to 4 years,none of the patients recurred. Conclusions Endoscopic surgery using low-temperature plasma radiofrequency for nasopharyngeal angiofibroma has many advantages such as less bleeding and total tumor removal. It is a minimally invasive surgical method for nasopharyngeal angiofibroma.

Objective To establish whether Coblation is a suitable modality for removal of early glottic carcinoma. Methods Fourteen patients with early glottic carcinoma (Tis-T2) without lymph node metastasis underwent resection of laryngeal cancer lesions using transoral endoscopic coblation (TEC),without pre- or post-operative radiotherapy and chemotherapy. Results No severe complication such as bleeding and dyspnea ocuurred in the cases. Only mild postoperative pain happened to the patients. All patients could eat and pronunce on the surgery day. With following-up of 25-37 months, no recurrence was observed in 13 cases. One case, with poorly differentiated squamous cell lesion in the anterior commissure invading subglottic, recurred 3 months postoperatively. The patient received the re-resection of laryngeal lesion by coblation, but another recurrence happened to the patient 6 months postoperatively, and then was cured by partial laryngectomy, with recurrence-free survival 21 months postoperatively. Conclusion The observation suggests that transoral endoscopic coblation is a reliable and safe modality for the resection of early glottic carcinoma.

OBJECTIVE: To study the efficacies of intratympanic prednisolone and dexamethasone injection for the subjective tinnitus. METHOD: A prospective study was designed to compare the efficacies of intratympanic prednisolone injection, intratympanic dexamethasone injection and carbamazepine by oral administration for subjective tinnitus. Seventy-three cases (78 ears) with subjective tinnitus for more than one month and treated by conservative therapy (such as vasodilator agent, Vitamin B, etc. by oral intake. ) were involved. The patients were randomized into 3 groups. Thirty-four cases (35 ears) were included in prednisolone group, 18 cases (18 ears) in dexamethasone group with intratympanic injection of prednisolone or dexamethasone, and 21 cases (25 ears) in carbamazepine group as a control group with oral administration of carbamazepine. All of the cases in intratympanic perfusion group were injected twice in the first week, then once a week consecutively. The patients were acupunctured 4-5 times in the whole course of treatment. All of the cases accepted Betahistine Mesylate, Mecobalamin and Vitamin B1 by oral intake at the same time. Pure tone audiogram and tinnitus matching were tested before the treatment immediately after the course of treatment, and were tested again after half a year's following up. RESULT: All of the cases accepted the whole treatment and were followed up for half a year successfully. The effective rate of the prednisolone group, dexamethasone group and the carbamazepine group was 48.6%, 33.3%, 44.0%, respectively; the control rate half a year after the treatment was 45.7%, 27.8%, 36.0%, respectively. There was no statistically significant difference in the effective rate and control rate between intratympanic perfusion group and carbamazepine group. There is a statistically significant difference both in the effective rate and the control rate between the prednisolone group and the dexamethasone group. Prednisolone may be better than dexamethasone in intratympanic perfusion for subjective tinnitus. CONCLUSION Intratympanic steroid injection has a positive effect on subjective tinnitus and may be considered to be an alternative treatment to subjective tinnitus.
Adolescent ; Adult ; Aged ; Carbamazepine ; administration & dosage ; therapeutic use ; Dexamethasone ; administration & dosage ; therapeutic use ; Drug Administration Routes ; Female ; Humans ; Male ; Middle Aged ; Prednisolone ; administration & dosage ; therapeutic use ; Prospective Studies ; Tinnitus ; drug therapy ; Treatment Outcome ; Young Adult