Objective To investigate the effects of angiotensin Ⅱ (Ang Ⅱ ) and angiotensin Ⅱ type 1 receptor antagonist (AT1RA) on the transforming growth factor-β1 (TGF-β1) synthesis and tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA expression of in vitro cultured hepatic stellate cells (HSCs). Methods HSC-T6 rat hepatic stellate cell line was selected as the study model of the activated hepatic stellate cells. Cultured HSCs were randomized into control group, Ang Ⅱ group, AT1RA group and Ang Ⅱ AT1RA group. Cell culture medium was used to detect the TGF-β1 level by ELISA method. HSCs were harvested to measure the TIMP-1 mRNA expression by RT-PCR. Results TGF-β1 level of control group, Ang Ⅱ group and AngⅡ AT1RA group in cell culture medium was (7.531 ±0. 654) pg/mL, (9. 855± 1. 485)pg/mL and (7.719 ± 0.329) pg/mL respectively, Ang Ⅱ group higher than control group (P ＜ 0.05 ), Ang Ⅱ AT1RA group lower than Ang Ⅱ group (P ＜ 0. 05 ). TIMP-1 mRNA expression level of control group, Ang Ⅱ group and Ang Ⅱ AT1RA group in HSCs was 3. 387 ± 0. 042, 4.870 ± 0.061 and 3. 837 ± 0. 042 respectively, Ang Ⅱ group higher than control group ( P ＜ 0. 05 ), Ang Ⅱ AT1RA group lower than Ang Ⅱ group (P ＜ 0. 05). Conclusion AngiotensinⅡ can increase the TGF-β1 synthesis and TIMP-1 mRNA expression of hepatic stellate cells, while all these effects are inhibited by angiotensinⅡ type 1 receptor antagonist.

OBJECTIVE To approach the nursing methods for protecting of the unilateral lung in eldly patients with severe pulmonary infection of both-sides after operation.METHODS The patients which had a relatively better function in one side ie.with a higher Sat-O2 value were selected.Using postural drainage and sputum aspiration in protecting the unilateral lung,turning the body over quickly and knocking the back of the body on the other-side of the lung were adopted to gradually recovered the pulmonary function side-by-side.RESULTS Through the new nursing model the pulmonary function and the Sat-O2 value could be improved.CONCLUSIONS When the both-sides of the pulmonary infected severely,nursing the unilateral lung at the beginning by using postural drainage,turning the body over quickly and knocking the back in the other side of the lungs,can efficiently speed up the proceeding of rehabilitation of pulmonary infection.

This paper reports the postoperative managenment of 58 cases who had various types of congenital heart disease and received artificial valves due to rheumatic heart disease in our institute from 1982 to 1984. Vaso-active agents were administered with the monitoring of the cardiac function. According to the hemodynamic parameters and the criterion for clinical typing, the vaso-active agents therapy can provide a precise prognosis and the therapy is of certain value in cardiac surgery.

Agarases are glycoside hydrolases.They are grouped into?and?types,which hydrolyze?-1,3 linkages and?-1,4 linkages respectively.The paper is about advance in research of agarase including the research of biology,the classcification,the crystal structure,the catalysis mechanism and application of agarases.

Objective:To study the clinical value and telomerase activity of malignant ascites treated by lifein combination with cisplatin.Methods:48 patients with malignant ascites were divided randomly into two groups.Treatment group of 28 cases were treated with lifein and cisplatin,control group of 20 cases were treated with cisplatin alone.Telomerase activity was detected by semi-TRAP assay before treatment,at the first week,the second week and the third week after treatment,respectively.Results:the effective rates of treatment group(89.3%) was much higher than that of control group (60%),P

Central Nervous System Viral Diseases ; complications ; virology ; Child ; Epstein-Barr Virus Infections ; complications ; virology ; Female ; Follow-Up Studies ; Herpesvirus 4, Human ; Humans ; Lymphocytosis ; etiology ; virology ; T-Lymphocytes

Objective Hypotension can be induced by sodium nitroprusside(SNP) combined with propofol more easily and with less amount of each drug. But both SNP and propofol were reported to inhibit platelet aggregation. The purpose of present study was to investigate the effect of controlled hypotension induced by SNP alone or in combination with propofol on human platelet aggregation. Methods Fifty-six ASA Ⅰ -Ⅱ patients (30 male, 26 female), aged 20-54 (36.0 ? 11.2) years and weighing 42-79 (67.6?14.3)kg, undergoing elective neurosurgery were randomly assigned to one of four groups: A control group ( n = 14) ; B propofol group ( n = 14) ; C SNP group( n = 14) and D SNP + propofol group ( n = 14) . The patients were premedicated with luminal 0.lg and atropine 0.5 mg. Anesthesia was induced with midazolam 0.2 mg ?kg-1 , fentanyl 5 ?g?kg-1 and vecuronium 0.1 mg?kg-1 and maintained with isoflurane inhalation and intermittent boluses of fentanyl and vecuronium. In group A and B no hypotension was induced. In group C and D hypotension was induced by 0.01 % SNP infusion (0.5-5 ?g?kg-1?min-1 ) alone or combined with propofol infusion(2-3 mg?kg-1?h-1 ) . As soon as the dura was cut, hypotension was induced. MAP was reduced by 30 % and maintained at ( 67.80 ? 9.64 ) mm Hg on average. Radial artery was cannulated for continuous BP monitoring. In order to avoid the effect of colloid and homologous transfusion on platelet function, only Ringer' s lactate was infused during operation in the four groups. Blood samples were taken from peripheral vein before skin incision, 30min and 1h after hypotension was induced and 2h after surgery for determination of platelet aggregation, prothrombin time and plasma level of NO2 -/NO3- .Results Platelet aggregation was significantly inhibited in group C and D at 30 min and Ih after induction of hypotension as compared with the baseline (before operation) . There was significant difference in the inhibition of platelet aggregation among the four groups. The inhibition was greatest in group D. There was no significant change in prothrombin time after induction of hypotension in the four groups. Plasma level of NO2-/NO3 - increased significantly at 30 min and 1h after induction of hypotension in group C and D. Conclusions SNP combined with propofol has inhibitory effect on human platelet aggregation during controlled hypotension and increased in plasma NO2 -/NO3-level may be the mechanism.

Objective:To compare the assessments on hyperkinetic syndrome of ADHD between teachers and psychiatrists,in order to determine their roles in our later study about ADHD.Method:Pediatric psychiatrists from Guangzhou psychiatric hospital and pre-occupational or experienced teachers from Fangcun elementary school(30 in each group)rated the presence and degree of hyperactive-disruptive behaviors in a standardized videotape,showing one 7-year-old boy who participated in individual and group activities.The results were compared between each group.Results:There was no significant difference on most of the assessments between psychiatrists and experienced teachers.The assessments of pre-occupational teachers had significant differences to the results of the above two groups.Conclusion:The assessments of experienced teachers may be used as very important information on the early diagnosis and the assessments of effectiveness of medical intervention for ADHD children.

AIM: To explore the feasibility of culturing human umbilical vein endothelial cells ( HUVEC ) on acellular corneal stroma and performing the posterior lamellar endothelial keratoplasty ( PLEK ) treating corneal endothelial decompensation.
METHODS: Thirty New-Zealand rabbits were divided into three groups randomly, 10 rabbits for experimental group, 10 for stroma group and 10 for control group. Corneal endothelial cells were removed to establish animal model of corneal endothelial failure. PLEK was performed on the rabbits of experimental group and stroma group, and nothing was transplantated onto the rabbits of control group with the deep layer excised only. Postoperative observation was taken for 3mo. The degree of corneal edema and central corneal thickness were recorded for statistical analysis.
RESULTS:Corneas in experimental group were relieved in edema obviously compared with that in stroma group and the control group, and showed increased transparency 7d after the operation. The average density of endothelial cells was 2 026. 4±129. 3cells/mm2 , and average central corneal thickness was 505. 2±25. 4μm in experimental group, while 1 535. 6±114. 5μm in stroma group and 1 493. 5±70. 2μm in control group 3mo after operation.
CONCLUSION:We achieved preliminary success in our study that culturing HUVEC on acellular corneal stroma and performing PLEK for corneal endothelial decompensation. HUVEC transplanted could survive in vivo, and have normal biological function of keeping cornea transparent. This study provides a new idea and a new way clinically for the treatment of corneal endothelial diseases.

Objective To research the risk factors of new-onset atrial fibrillation (AF) during hospitalization in patients with acute myocardial infarction.Methods Five hundred and one patients first diagnosed with acute ST-segment elevation myocardial infarction who accepted primary coronary intervention from January 2012 to May 2014 in the Second Hospital of Tianjin Medical University were selected.These patients were divided into non-AF group and new-onset AF group depending on whether happened new-onset AF during hospitalization.Data of the patients in acute myocardial infarction complicated with new-onset atrial fibrillation were collected.Univariate and multivariate logistic regression analysis were used to determine the predictors of new-onset AF.Results AF occurred in 49 cases of the 501 (9.8％) patients diagnosed as acute myocardial infarction.Logistic regression analysis showed that admission heart rate(OR =1.019,95％CI:1.004-1.034,P =0.012),left atrial diameter (OR =1.086,95％ CI:1.021-1.156,P =0.009),previous stroke (OR =2.060,95％ CI:1.029-4.123,P =0.041) were independent risk factors for new-onset AF in acute myocardial infarction.Use of β-blockers before coronary intervention treatment can reduce the risk of new-onset atrial fibrillation(OR=0.222,95％CI:0.064-0.768,P=0.017).Conclusion Admission heart rate and left atrial diameter are independent predictors of new-onset atrial fibrillation,and β-blockers in early stage can effectively reduce the incidence of atrial fibrillation.