Co-occurrence network of major MeSH terms and all MeSH terms was constructed respectively with MEDLINE-covered pharmacological literature as its example.The degree, closeness and betweenness of centrality in different nodes of the two co-occurrence networks were analyzed , the distribution of scale-free properties in the two co-occurrence networks was tested, the small-world effect of the two co-occurrence networks was identified by comparing their overall properties ( average distance and clustering coefficient ) , which showed the scale-free properties and small-world effect of the two co-occurrence networks.The co-occurrence network of all MeSh terms could thus be used in analysis of network properties while that of major MeSH terms could thus be used in analysis of subject contents.

After the basic properties of literature-related citation network, co-authorship network and co-words network were analyzed and the advances in their application research were summarized in aspects of their construc-tion methods, size and research depth, it was pointed out that article similarity networks could be constructed using the article similarity algorithm, and their basic properties and features were analyzed.

Text mining provides a new approach for drug repositioning, and the emerging databases and their corresponding tools provide more and more convenience for drug repositioning by text mining.The methods and tools for studying drug repositioning by text mining and their successful application examples were thus described in this paper in order to provide reference for the researchers interested in this field.

Objective To study the effects of interferon gamma (IFN-?) on pulmonary fibrosis induced by bleomycin in rats and the related mechanisms. Methods A total of 90 rats were randomly divided into 3 groups (n=30 in each group): normal control group, pulmonary fibrosis group, and IFN-? treated group. On days 1, 3, 7, 14, and 28 after bolemycin treatment, 6 rats in each group were sacrificed. PO 2 was determined. The lungs and serum were collected for histopathological and electron microscopic examinations. The contents of transforming growth factor ? 1 (TGF-? 1) and fibronectin (FN) were determined by immunohistochemistry, and the contents of IL-4 and surfactant protein A (SP-A) were detected by ELISA and Western blotting, respectively. Results PO 2 level increased significantly (P

Olfaction is one of the ancient sensing capabilities and plays an important role in monitoring environment. Although olfactory loss is common, it's obviously underappretiated by medical community generally. In order to help patients with those problems, the author gives an brief review about the characters of common etiologies, treatment and prognosis of Olfactory Dysfunction. It's concluded that most usual causes resulting in dysos- mia are upper-respiratory-tract infections, trauma, and chronic rhinosinusitis; and our understanding of the olfaction mechanism grows, but frustratingly, aside from the possible therapeutic potential of systemic steroids, no method has been proved to have solid evidence for curing olfactory loss; so we need more new basic and clinic research to develop effective therapeutic intervention.
Chronic Disease ; Humans ; Olfaction Disorders ; etiology ; therapy ; Prognosis ; Respiratory Tract Infections ; Sinusitis ; Smell

Definiting the workflow and key link of the risk management in medical laboratory by FMEA.Identifying risk factors of the workflow and key link of blood coagulation test by the criteria for laboratory accreditation , such as ISO15189 recognition criteria and CAP laboratory accreditation inspection . Through the evaluation of the blood coagulation test , effective corrective actions and examining performance data periodically , the quality of the blood coagulation test can be improved continuously.

Purpose: To observe the therapeutic effect of plum-blossom-magnet-needle synthetic therapy on benign prostatic hyperplasia (BPH). Method: 219 cases of BPH patients were randomly divided into two groups. There were 149 cases in treatment group, which treated by pressing, needling with plum-blossom-magnet-needle and putting effect-increasing pads on Prostate Reflex Area, bilateral Zhongliao (BL 32), Neck 1, Neck 7, Mingmen (GV 4) and Yongquan (KI 1). There were 70 cases in control group, which treated by orally taken Terazosin Hydrochloride (Gaoteling) tablets, 2 mg/time, twice a day. The therapeutic course was 4 weeks.After one therapeutic course, the therapeutic effect was evaluated. Results: The marked effect rate was 63.3% and total effective rate was 91.8%; in the control group, there were no markedly effective cases and the total effective rate was 72.7%. There was a significant difference between two groups (P<0.05). Conclusion: The plum-blossom-magnet-needle synthetic therapy had a significant therapeutic effect for the treatment of benign prostatic hyperplasia (BPH), so it was a safe and effective therapy for BPH.

The high frequency subject terms were extracted from the PubMed-covered papers published from January 2000 to March 2015 on text mining of biomedical field to generate the matrix of high frequency subject terms and their source papers.The co-occurrence of high frequency subject terms in a same paper was analyzed by clustering analysis.The hotspots in text mining of biomedical field were analyzed according to the clustering analysis of high frequency subject terms and their corresponding class labels, which showed that the hotspots in text mining of bio-medical field were the basic technologies of text mining, application of text mining in biomedical informatics and in extraction of drugs-related facts.

Objective:To search for carrier of short active peptide with binding to human CD59 of the surface of HeLa cell.Methods:Ph. D. 12 display peptide library was biopanning with HeLa cell high-expressing CD59. After competitive test, positive phage clones were identified by sandwich ELISA and sequenced.Results:10 phage clones were identified to have higher combination with CD59.Conclusion:The sequence of peptide with binding to CD59 has been obtained.That might lay a basis for design of short peptide of CD59 on tumor sneaking through.

AIM: To investigate the role of resveatrol (RES) among red wine on the proliferation, activity of NF-?B and the expression of monocyte chemotactic protein-1 (MCP-1) induced by xanthine and xanthine oxidase in cultured rabbit aortic smooth muscle cells (SMC). METHODS: SMC proliferation was examined by 3-4, 5-dimethylthiazol-2-yl-2, 5-diphenylte tra zoliumbromide (MTT) metabolism, activity of NF-?B, the protein and mRNA expression of MCP-1 were detected by electrophoretic mobility shift assay (EMSA), immunohistochemitry and in situ hybridyzation in cultured rabbit aortic SMC. RESULTS: 100 ?mol/L-200 ?mol/L resveatrol, an effective composition in red wine, was confirmed to inhibit MTT metabolism and the activity of NF-?B as well as the protein and mRNA expression of MCP-1 in rabbit aortic SMC, which were promoted by the oxygen free radicals induced by xanthine and xanthine oxidase. CONCLUSION: Resveatrol may antagonist oxygen free radicals-induced proliferation and the activity of NF-?B as well as protein and mRNA expression of MCP-1 in cultured rabbit aortic SMC, which might play an important role in preventing atherosclerosis. [