Objective To find a new method of tangential view of patello femoral joint. Methods 62 cases of lateral knee radiographs were studied. The knee flexion angles, patello femoral angles, patello tibial angles and frontal patello tibial angles were measured and their relationships analyzed. The knee flexion angles and the patello femoral angles had linear relationship with a linear equation = 2 + 0.8x . The angles of posterior patellar line with tibial long axis and frontal tibial line were 3.3? and 12.7?respectively. According to the measurement results, an accessary positoning table was designed. The patients′ patello femoral joints were examined with the knee flexed at an angle of 30?. The patients were in supine position and the central X ray was angled at 73?vertically toward the film. Results 58 patello femoral joints of 40 patients were radiographically studied (10 males, 30 females) .All radiographs were classified into 4 degrees as Grade A, B, C and D. Eighteen cases were Grade A (31%), 30 cases were Grade B(52%), 8 cases were Grade C(18%)and 2 Grade D(3%).The reason for Grade D was due to small or negtive anterior patellotibial angles. Conclusion This new method of tangential view of patello femoral joint is accurate, comfortable and simple and could be used as routine examination for patello femoral joint diseases.

Objective To analyze multislice CT characteristics of intestinal obstruction transition zone caused by different patholo-gy.Methods 67 cases of intestinal obstruction were collected in our hospital.Multislice spiral CT scan combined with multi-plane re-organization (MPR)of the bowl obstruction transitional zone were analysed.Results Among 67 cases of intestinal obstruction,there were 28 cases of simple mechanical intestinal obstruction,1 7 cases adhesion intestinal obstruction,5 cases paralytic intestinal obstruc-tion,4 cases internal hernias and 4 cases mixed intestinal obstruction.Conclusion Intestinal canal transitional zone and arounding tis-sues of intestinal obstruction have certain characteristics for the same pathology which can be clearly displayed by multislice CT scan combined with MPR.

The long-year program for medical education is the major way to train the high level medical professionals.This paper colleeted the research papers on this topic published in Chinese journals in recent years,extracted their key words and counted the high frequent key words According to the co-occurrence of a pair of key words in one paper.these high frequent keywords were clustered into groups.The research structure and main directions of the long-year program were outlined as the study of macro-planning of long-year program,the study of teaching models and teaching system,study of teaching practice.These results could be a useful reference for the medical educational researchers and managers.

Objective:To compare the visual quality of patients with astigmatic multifocus intraocular lens (ART IOL) and astigmatic monofocus IOL (Toric IOL) after implantation.Methods:A prospective, non-randomized, controlled study was conducted to include 72 eyes of 72 cataract patients with corneal astigmatism (1.0-2.5 D) who were scheduled to receive phacoemulsification combined with IOL implantation in Chaoju Eye Hospital of Inner Mongolia from July 2017 to October 2018.Patients were divided into ART IOL group (37 eyes) and Toric IOL group (35 eyes) according to different IOLs implanted.All patients were followed up for 1 year after surgery.The main and secondary outcome indicators were compared.The main outcome indicators included distance (5 m), intermediate (80 cm), and near (40 cm) uncorrected visual acuity and best corrected visual acuity, defocus curve, contrast sensitivity, postoperative IOL rotation and residual astigmatism, while the secondary outcome indicators included visual subjective symptoms and spectacle independence rate.The above outcome indicators were compared between the two groups.This study followed the Declaration of Helsinki.The study protocol was approved by the Ethics Committee of Chaoju Eye Hospital of Inner Mongolia (No.CJYKLL202012).Written informed consent was obtained from each patient before surgery.Results:There was no statistically significant difference between the two groups in average uncorrected distance visual acuity and best corrected distance, intermediate and near visual acuity (all at P>0.05).The average intermediate and near visual acuity in the ART IOL group was significantly higher than that in the Toric IOL group, showing statistically significant differences (both at P<0.05).The defocus curve of the ART IOL group presented a double peak, which reached the peak at the far focus (0.0 D) and near focus (-2.5 D), respectively.The visual acuity range from 1.5 to-3.0 D was above 0.2 logarithm of the minimum angle of resolution (LogMAR), and the focus depth range was 4.5 D (1.5 to -3.0 D).The defocus curve of the Toric IOL group had only one crest, which was located at best corrected visual acuity (0.0 D).The visual acuity reached 0.2 LogMAR or above were from 1.0 to -1.0 D, and the focal depth range was 2.0 D (1.0 to -1.0 D).There was no statistically significant difference in contrast sensitivity at different spatial frequencies between the two groups in bright light, bright flashing light and dark light (all at P>0.05), while the contrast sensitivity of the ART IOL group at high dark flashing light frequency was lower than that of the Toric IOL group, showing statistically significant difference ( P<0.05).In the ART IOL group and the Toric IOL group, the average residual astigmatism after surgery was (-0.47±0.51)D and (-0.49±0.42)D, respectively, with no statistically significant difference ( t=0.408, P>0.05).There was no significant difference in postoperative IOL rotation between the two groups ( t=1.906, P>0.05).The total postoperative spectacle independence rate in the ART IOL group was 97.30%(36/37), which was higher than that in the Toric IOL group (17.14%, 6/35), showing statistically significant difference ( χ2=15.320, P<0.05). Conclusions:Both Toric IOL and ART IOL can better correct corneal astigmatism and improve patients' distance visual acuity, but ART IOL implantation can provide better distance, intermediate and near visual acuity, and higher spectacle independence rate.

Brucellosis ; China ; Epidemics ; Humans

Objective:To better understand the karyotype distribution ofthe patients with Down's syndrome(DS)who have been finally diagnosed in our hospital.To compare the strengths of G-banding karyotype analysis and FISH(Fluorescence In Situ Hybridization, FISH)in diagnosing the DS patients with the Chromosome mosaicism and Calculating the percentage of abnormal karyotypes.Methods:Blood of the patients who were likely to suffer from DS was selected for G-banding karyotype analysis to describe the karyotype distribution.5 of the finally diagnosed patients with the chromosome mosaicism were randomly selected for FISH to compare the percentage of abnormal karyotypes of the two methods.Results:287 DS patients were diagnosed,91.29%of whom had the simple karyotype.Among the patients with the translocation karyotype, 11 D/G translocation karyotype were detected,accounting for 73.33%,while 4 G/G translocation karyotype were diagnosed, constituting 26.67%.One patient with both of the translocation karyotype and the mosaicism karyotype was detected in our research,which was uncommon in recent report.The results of FISH and G-banding karyotype analysis were consistent.There was no significant difference of the abnormal karyotype rates for DS patients with the chromosome mosaicism by G-banding karyotype analysis and FISH.Conclusion:The simple karyotype is the main karyotype of the DS patients in our research.Among the translocation karyotype,the D/G kind is common.DS patients can be diagnosed efficiently by G-banding karyotype analysis,but we still suggest to use FISH to get the proportion of the abnormal cell for the patients who have less triploid cell and slighter clinical manifestation.

Objective:To investigate the relationships between Leu72Met polymorphism ofpreproghrelin gene and type 2 diabetes mellitus (T2DM)in Han ethnics ofGuangzhou.Methods:We conducted a case-control study using 95 non-T2DM healthy individuals as controls (the control group)and 102 individuals diagnosed with T2DM as patients(the T2DM group)from populations of Han ethnics in Guangzhou,in order to detect genotypes of Leu72Met polymorphism of preproghrelin gene within each group,and to compare the differ-ences of genotypic and allelic frequencies between the two groups.Various molecular biology techniques were employed in the study in-cluding target gene segments amplification using the polymerase chain reaction(PCR).After amplification,digestions of PCR products were carried out using restriction enzyme BseNⅠand fragments obtained were detected using Native-PAGE in conjunction with a highly sensitive silver stain method.Results:In the healthy control group,the genotypes CC and AC were detected in 68 and 27 individuals,re-spectively,while no genotype AA was detected in this group.Their corresponding genotypic frequencies were 0.716,0.284,and 0,re-spectively.In the T2DM group,the genotypes CC,AC,and AA were detected in 71,25,and 6 cases,respectively.Their corresponding genotypic frequencies were 0.696,0.245,and 0.059,respectively.Allele frequencies detected were in equilibrium with the Hardy-Wein-berg equation.However,there were no significant differences between the control group and the T2DM group in terms of genotypic and al-lelic frequencies.Conclusion:There are no significant relationships between Leu72Met polymorphism of preproghrelin gene and T2DM in Han ethnics of Guangzhou.However,genotype AA was detected in the T2DM group but not in control,which suggested that allele A may play certain roles in T2DM development.Further investigations certainly would be worth pursuing.

Two animal models were established in this experiment to investigate the relationships between muscle damage and plasma activity of CK and its isoenzymes of rats after different modes of exercise.Model 1: untrained male Sprague-Dawley rats undertook swimming exercise of three different durations:25 minutes, 50 minutes and exhaustion. Model 2:after 2-week′s downhill running, male Wister rats undertook a 3-graded downhill running finally. Plasma and soleus muscle samples were taken to observe the changes of CK, CK isoenzymes and muscle damage. The linear correlation coefficients between plasma CK activity, CK-MM% and the Z-band abnormity rate of soleus muscle immediately after swimming in model 1 were 0.875 ( P

Objective To investigate the antibacterial and antifungal metabolites from the fermentation broth of Streptomyces sp.M095 and classify the strain M095.Methods The structure was evaluated by spectral(ESI-MS,EI-MS,~(1)H-NMR,~(13)C-NMR and APTNMR) analysis.And the strain M095 was analysed with the characteristic of the culture and modality and the sequence of 16S rDNA.Results An antibiotic was found to have the remarkable activity to Staphylococcus aureus and filaceous fungi Mucor miehei(T 284).And its structure was identified as holomycin.The strain M095 was classified as maybe a variety belongs to Streptomyces Globisporus group.Conclusion The activity of holomycin to fungi was reported for the first time in this paper.The antimicrobial spectrum of holomycin was broadened.

BACKGROUND:High-energy patelar comminuted fracture significantly increased. Single fixation cannot meet the requirement of anatomical repositioning of the patela, multiple-point strong fixation, and early functional exercise. OBJECTIVE:To compare the clinical effect of adjustable patela claws + absorbable suture and Kirschner wire tension band in the repair of comminuted patelar fractures. METHODS: A total of 57 patients with comminuted patela fracture were randomly selected and randomly assigned to two groups. 29 cases received open reduction and adjustable patela claws + absorbable suture fixation. 28 cases received open reduction and Kirschner wire tension band fixation. Operative time, incision length, fracture healing time, postoperative complications and Hospital for Special Surgery knee function scores were compared between the two groups, and then statistical analysis was conducted. RESULTS AND CONCLUSION:No significant difference in incision length and operative time was detected between the adjustable patela claws + absorbable suture group and Kirschner wire tension band group. Significant differences in healing time, incidence of complications and knee function after treatment were observed between the two groups, and above indications were better in the adjustable patela claws + absorbable suture group than in the Kirschner wire tension band group (P < 0.05). These results confirm that compared with the Kirschner wire tension band, adjustable patela claws + absorbable suture in repair of patelar comminuted fracture has some advantages such as short healing time, less postoperative complications, as wel as good functional recovery of knee joint. The fixation is accorded with biomechanical principle of internal fixation, simple to operate, firmly fixed, and has good prospects.