The present study was performed to evaluate the effect of tetracycline-HCL on the change of implant surface microstructure according to application time. Implant with pure titanium machined surface, double coated FBR(R) surface and oxidized CellNest surface were utilized. Implant surface was rubbed with 50mg/ml tetracycline-HCL solution for 1/2, 1, 1 1/2, 2 and 2 1/2min. respectively in the test group. Then, specimens were processed for scanning electron microscopic observation. The results of this study were as follows. 1. Both test and control group showed a few shallow grooves and ridges in pure titanium machined surface implants. There were not significant differences between two groups. 2. The double coated FBR(R) surfaces showed fine crystalline structures. The roughness of surfaces conditioned with tetracycline-HCL was lessened relative to the application time. 3. The oxidized CellNest surfaces showed the porous structures. The surface conditioning with tetracycline-HCl influenced on its micro-morphology. In conclusion, the detoxification of the affected implant surface with 50mg/ml tetracycline-HCL should be applied respectively with different time according to various implant surfaces.
Crystallins ; Titanium

No abstract available.
Crystallins* ; Wounds and Injuries*

Cardiomyopathies ; Crystallins ; Humans

PURPOSE: Surface color is one of the main criteria to obtain an ideal esthetic. Many factors such as the type of the material, surface specifications, number of firings, firing temperature and thickness of the porcelain are all important to provide an unchanged surface color in dental ceramics. The aim of this study was to evaluate the color changes in dental ceramics according to the material type and glazing methods, during the multiple firings. MATERIALS AND METHODS: Three different types of dental ceramics (IPS Classical metal ceramic, Empress Esthetic and Empress 2 ceramics) were used in the study. Porcelains were evaluated under five main groups according to glaze and natural glaze methods. Color changes (DeltaE) and changes in color parameters (DeltaL, Deltaa, Deltab) were determined using colorimeter during the control, the first, third, fifth, and seventh firings. The statistical analysis of the results was performed using ANOVA and Tukey test. RESULTS: The color changes which occurred upon material-method-firing interaction were statistically significant (P<.05). DeltaE, DeltaL, Deltaa and Deltab values also demonstrated a negative trend. The MC-G group was less affected in terms of color changes compared to other groups. In all-ceramic specimens, the surface color was significantly affected by multiple firings. CONCLUSION: Firing detrimentally affected the structure of the porcelain surface and hence caused fading of the color and prominence of yellow and red characters. Compressible all-ceramics were remarkably affected by repeated firings due to their crystalline structure.
Ceramics* ; Crystallins ; Dental Porcelain ; Fires*

Matrix stone is a rare form of urinary tract concretion and composed primarily of matrix material, approximately 65% by weight. This must be distinguished from the various diseases representing a radiolucent mass within the renal collecting system. We report a case of matrix stone in 32 year-old female patient who had a crystalline stone of con. tralateral kidney with the review of literatures.
Adult ; Crystallins ; Female ; Humans ; Kidney ; Urinary Tract

It has been known that ultraviolet B(UVB) light made an oxidative damage to lens proteins, lipids and nucleic acids to induce lens opacity. The aim of the study was to investigate the effect of bendazac lysine salt (Bendaline) tot the experimental cataract developed by UV irradiation. Forty rats were exposed to 0.1mW/cm2 of UVB radiation in the range 300-320 mm for 24 hours per day. Five control rats were not exposed UVB radiation. During the investigative period, we measured lens opacity with Scheimpflug camera every other week. Rats were divided into 9 groups according to the duration of UV radiation and initial time of bendazac lysine medication. Bendazac lysine was administered orally by 25mg/kg per day for 2 months. The opacities on anterior cortex, nucleus and posterior capsule began to appear 4 months after UVB irradiation. The longer duration of radiation, the more severe opacity of lens was observed, especially at the layers of posterior supranucleus, posterior cortex and posterior capsule and in the opacity area by retroillumination image. After UVB induced cataract was developed, the lens opacity was not changed nevertheless stop the UV irradiation. Lens opacity of bendazac lysine-treated groups was not severer than that of no medication groups. There were less opacities on 4 month irradiated group rather than 6 month irradiated group at the layers of nucleus and posterior cortex and in the opacity area. Anticataract action of bendazac lysine was effective in earlier cataract. In the group of bendazac lysine medication with UVB irradiation on same time, the prophylactic evidence of bendazac lysine was not observed.
Animals ; Cataract* ; Crystallins ; Lysine* ; Nucleic Acids ; Rats*

It is difficult to get sufficient roughness on titanium implant surface using traditional electrochemical treatments. In this study, we have developed a new method which provides a hybrid structured titanium surface having micro/nano roughness using electrochemical treatment in NaCl electrolyte and hydrothermal treatment. Titanium disks were anodically oxidized (ANO) in 0.15M NaCl electrolyte by applying positive electric pulses. The oxide compounds loosely attached to the surface were removed by ultrasonic cleaning (ANO group). These specimens were hydrothermally (HT) treated in an alkaline solution (ANO-HT group). ANO group showed the dimpled grain surfaces with a diameter of approximately 30 µm, and its roughness (Ra) was about 2.4 µm. The nano-sized crystallites which had an anatase TiO₂ crystalline structure were uniformly distributed on the surface of ANO-HT group. This group still retained high roughness (~2.7 µm) similar to ANO group and showed high hydrophilicity. Titanium surface with high roughness and hydrophilicity was fabricated using new electrochemical treating method and hydrothermal treatment. This surface modification method could be used for enhancing the osteoconductivity of the titanium implants.
Crystallins ; Hydrophobic and Hydrophilic Interactions ; Methods ; Titanium ; Ultrasonics

PURPOSE: Recently, bioceramics have become popular as a substitute graft material for reconstruction of bony defect after trauma or tumor surgery. Among the bioceramic materials, hydroxyapatite(HA) is favored due to its biocompatibility. HA scaffold is composed of the interconnected reticular framework, macropores and micropores. Macropores play an important role in cell migration, nutrients supply and vascular ingrowth. On the other hand, a number of micropores less than 10micrometer form an irregular surface on HA scaffolds, which prevents the osteoblast from adhering and proliferating on the surface of HA scaffold. METHODS: In this study, three different groups were designed for comparison. In the first group(group A), conventional method was used, in which HA pellet was applied without surface pretreatment. The second group (group B) was given a HA pellet that has been coated with crystalline HA solution prior to application. In the third group(group C), the same method was used as the second group, where the pretreated HA pellet was heated(1250degrees C, 1 hour) before application. Osteoblast-like cells(2x10(4)/mL) were scattered onto every pellet, then they were incubated in 5% CO2 incubator at 37degrees C for twelve days. During the first three days, osteoblast cells were counted using the hemocytometer daily. ALP activity was measured on the 3, 6, 9 and 12 culture days using the spectrophotometer. RESULTS: Under SEM, group A showed a surface with numerous micropores, and group B revealed more rough crystal surface. Group C revealed a fused crystal appearance and flattened smooth surface. In proliferation and ALP activity of osteoblast cells, group C showed better results compared to group B. Group A which lacks pretreatment of the surface showed less osteoblast proliferation and ALP activity than group C, but showed better results than group B. CONCLUSION: We found that crystallized HA with heat treatment method enhances the osteoblasts proliferation and differentiation on the surface of HA pellets.
Cell Movement ; Crystallins ; Durapatite ; Hand ; Hot Temperature ; Incubators ; Osteoblasts ; Transplants

On the developmental process of the lens opacification, some preliminary evidence suggested a defect in the pump mechanism of the lens membrane as one of the triggers for the formation of lens turbidity, eventually water penetration into the lens precedes the initial phase of cataract. Above the biochemical evaluation recognized that an apparent deficiency of Na-K ATPase may be involved in the initial factor of the hereditary mouse cataract. As a matter of one more fact, it found by the technique of lens implantation that the cataract formation of this type may by based on the inductive influences in the lens, not appeared by the outside surrounding of the lens. Additionally, at the developmental process or the cataract, the physico-chemical evidence regarding the changes of three dimensional structure and the aggregation of the soluble lens proteins may be effected in the immediate turbidity phenomenon of the lens. This report which is reviewed focusing on the membrane function delineates the approach to make finding the mechanism of the cataract using the some model expriment.
Adenosine Triphosphatases ; Animals ; Cataract ; Crystallins* ; Lens, Crystalline* ; Membranes ; Mice ; Water

Crystalline retinopathy is a tapetoretinal degeneration characterized by glistening yellow intraretinal crystals in the posterior pole, chorioidal sclerosis, and corneal crystalline dystrophy. We have recently encountered a 36 year-old female patient with bilateral intraretinal crystalline depositions who presented with a complaint of visual disturbance in her right eye for the last six months. Typical fundus findings of crystalline deposition in the posterior pole, tapetoretinal dysfunctions in the electrophysiologic tests and choriocapillaris filling defects in the fluorescein angiogram suggest that this the disorder is a widespread choriocapillaris and tapetoretinal disorder. There are no significant abnormal findings in serologic and radiologic examinations.
Adult ; Crystallins* ; Female ; Fluorescein ; Humans ; Retinitis Pigmentosa ; Sclerosis