1. Clinical and environmental isolates of Burkholderia pseudomallei from Brazil: Genotyping and detection of virulence gene
Tereza de Jesus Pinheiro Gomes BANDEIRA ; Débora de Souza Collares Maia CASTELO-BRANCO ; Marcos Fábio Gadelha ROCHA ; Rossana de Aguiar CORDEIRO ; Crister José OCADAQUE ; Manoel de Araújo Neto PAIVA ; Raimunda Sâmia Nogueira BRILHANTE ; José Júlio Costa SIDRIM ; Tereza de Jesus Pinheiro Gomes BANDEIRA ; Marcos Fábio Gadelha ROCHA ; Manoel de Araújo Neto PAIVA
Asian Pacific Journal of Tropical Medicine 2017;10(10):945-951
Objective To evaluate the genetic diversity of clinical and environmental isolates of Burkholderia pseudomallei (B. pseudomallei) recovered in Ceará Brazil, and screen these isolates for the presence of type three secretion system virulence gene. Methods Nineteen B. pseudomallei isolates (9 from clinical cases and 10 from soils) were analyzed. Random amplified polymorphic DNA was performed with primers OPQ-2, OPQ-4 and OPQ-16 to evaluate the genetic diversity, and type three secretion system gene was detected through polymerase chain reaction. Results Random amplified polymorphic DNA showed a genetic relatedness of approximately 50% among the tested B. pseudomallei isolates, which were grouped into two clades, of which the biggest ones comprised 18/19 isolates for primer OPQ-2, and 17/19 isolates for primer OPQ-16. Primer OPQ-4 grouped the isolates into three clades comprising 1/19, 3/19 and 15/19 isolates. Additionally, type three secretion system gene was detected in all tested isolates. Conclusions This was an effort to type B. pseudomallei strains from Ceará which is important for better understanding this pathogen, contributing for the epidemiological surveillance of melioidosis in this endemic region.
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