No abstract available.
Creatinine*

No abstract available.
Creatinine* ; Humans

No abstract available.
Creatinine* ; Plasma*

Creatinine (Cr) is a representative biomarker reflecting renal function. In this study, we compared serum Cr levels using Roche Modular D (Roche Diagnostics, Germany), Roche Cobas 8000 c702 (Roche Diagnostics), and AU5800 (Beckman Coulter, USA). In addition, we assessed the differences in Cr measurements using the Jaffe and enzymatic methods.METHODS: Precision, linearity, and methods were evaluated in accordance with CLSI guidelines. Serum Cr was measured by Modular D following the Jaffe method, and serum Cr was measured by Cobas 8000 c702 and AU5800, following the Jaffe and enzyme methods.RESULTS: All of the total coefficients of variations (CVs) were below 5%. Linearity was observed in the performance ranges evaluated (r>0.99, slope: 0.965 and 0.955). When Modular D and Cobas 8000c 702 were compared, the slope and y-intercept were 0.9928 (95% confidence interval [CI]: 0.9802 to 1.000) and -0.0156 (95% CI: −0.0200 to −0.0054), respectively. The slope and y-intercept were 0.9811 (95% CI: 0.9570 to 0.9951) and -0.0484 (95% CI: −0.0638 to −0.0297) when Modular D and Au5800 were compared. Serum Cr measured by Cobas 8000 c702 and AU5800 using the Jaffe method were 3.2% and 6.9% lower than the values measured by Modular D, respectively. Both Modular D and Cobas 8000 c702 showed acceptable accuracies.CONCLUSIONS: Serum Cr measurements using Cobas 8000 c702 and AU5800 were comparable to those measured by Modular D, and showed satisfactory precision and linearity; thus, these techniques could be useful for clinical laboratories.]]>
Creatinine ; Methods

No abstract available.
Creatinine* ; Humans ; Infant, Newborn*

OBJEVTIVE: To evaluate the peritoneal clearance of the middle molecule compared with that of the small molecule in incremental peritoneal dialysis(PD). METHODS: Peritoneal clearances of the creatinine and beta2-microgloblulin were compared in 57 continuous ambulatory PD patients with full dose 4 times exchange and in 54 incremental PD patients with 2 or 3 times exchange over 24 hours. The clearances were also compared when there were changes in the peritoneal dialysis regimen such as in the number of exchanges and dwelling time. RESULTS: Peritoneal creatinine clearance increased almost linearly along with the increase in the number of exchanges. In contrast, peritoneal clearance of beta2-microglobulin was 9.1+/-3.6 L/week, 8.8+/-4.4 L/ week, and 7.9+/-2.5 L/week respectively with 2, 3 and 4 exchanges per day, not different from each other. Peritoneal clearance of beta2-microglobulin did not change when there was an increase in the number of exchange from 2 to 3 times and 3 to 4 times over a period of 24 hours, whereas the peritoneal clearance of creatinine increased. Peritoneal clearance of beta2-microglobulin almost doubled from 5.4+/-2.7 L/ week with 2 times exchange over 12 hours per day, to 9.5+/-4.4 L/week with 2 times exchange over 24 hours, whereas the creatinine clearance did not change. CONCLUSION: In contrast to peritoneal clearance of small molecule which depends on the number of dialysate exchange, peritoneal clearance of middle molecule depends mainly on the total dwelling hours rather than the number of exchange per day in incremental PD. This can be another advantage of incremental PD since peritoneal clearance of middle molecules in incremental PD over 24 hours is comparable to that in full dose PD.
Creatinine ; Humans ; Peritoneal Dialysis*

Serum creatinine has been widely used clinically as an important index for kidney function. Kinetic Jaffe assay is used for serum creatinine analysis at about 80% of clinical laboratories in Korea. There are two major interferences when creatinine level is measured by kinetic Jaffe method. One is Jaffelike chromogen, which causes positive interference, and the other is bilirubin that creates negative interference. Positive interference created by Jaffelike chromogen can be easily corrected by subtracting 0.3 mg/dL (arithmetic compensation), which is average interference by Jaffelike chromogen in normal serum, from the measured creatinine value by kinetic Jaffe method. The interference created by bilirubin can be eliminated by rate blanking which corrects the rate of change in absorbance by bilirubin from the absorbance change by Jaffe reaction. Compensated rate-blanked Jaffe kinetic assay employs above two major corrections. In clinical laboratories currently using kinetic Jaffe Method, simple application of "compensated rate-blanked Jaffe kinetic assay" can determine serum creatinine values that minimizing major interferences without change of reagent.
Bilirubin ; Creatinine ; Kidney ; Korea

No abstract available.
Creatinine* ; Kidney Failure, Chronic*

Extracorporeal shock wave lithotripsy(ESWL) has become a major treatment modality for symptomatic renal stone disease. Although ESWL was proved to be effective in disintegrating stones it is known that some radiological evidence of transient renal malfunction could be possible after ESWL. However. it has been difficult to assess the renal damage quantitatively. We evaluated several basic physiologic parameters namely, total protein excretion, creatinine clearance and beta-2-microglobulin excretion in aliquots of 24 hour urine samples. ESWL was performed using Siemens Lithostar device. In a total of. 33 patients urine samples were obtained before, 1 day and 7 days after ESWL without prior manipulation. Our data showed that transient increased protein excretion and decreased creatinine clearance occur immediately after treatment, and return to pre-procedure levels within 7 days without a change in beta-2-microelobulin excretion after ESWL. Conclusively, renal damage induced by ESWL is thought to be transient and of limited magnitude of brief duration.
Creatinine ; Humans ; Lithotripsy* ; Shock*

No abstract available.
Creatinine* ; Humans ; Infant, Newborn*