1.Elaboration of protocol in dosage of ferulic acid in Radix angelicae sinensis by HPLC
Pharmaceutical Journal 2005;348(4):21-24
The extracted and purified ferulic acid residue from Radix angelicae sinensis was quantified by an established HPLC system composed of the following conditions: reversed phase column C-18 Purosphere 4.6mm 1D x 250 mm (5mm); mobile phase: acetonitrile/potassium dihydrophosphate 10 mM/L (15/85); pH: 4.5; flow rate: 1.2 mL/min; column temperature: 30oC. The results showed that the method can meet the following criteria: Rs > 1.5, RSD < 2%, N > 1 000, Tf in the range 0.8-1.2
Coumaric Acids
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Drugs
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Chinese Herbal
2.Systematic review on sodium ferulate for treatment of diabetic nephropathy.
Hong YIN ; Xiang YAN ; Ke-hu YANG
Chinese Journal of Integrated Traditional and Western Medicine 2009;29(11):970-974
OBJECTIVETo systematically review the efficacy and safety of sodium ferulate (SF) for the treatment of diabetic nephropathy.
METHODSBy computerized retrieving the Cochrane Library, MEDLINE, EMBASE, CNKI, VIP, CBM (theses, conference and internet materials), as well as data from internet materials regarding randomized controlled clinical trials of sodium ferulate for the treatment of diabetic nephropathy were collected completely. Data were strictly extracted using the simple evaluation method recommended in Cochrane Handbook and Meta-analysis was performed using Revman 5.0 software.
RESULTSFourteen randomized controlled trials involving 906 patients met the inclusion criteria. Meta-analysis showed that as compared with the control group, the effects in SF group were superior in terms of reducing urinary albumin excretion rate (UAER) at early stage [WMD = 16.08, 95% confidence interval (95% CI): 11.01 to 21.15] and clinical stage (WMD = 82.66, 95% CI: 66.95 to 98.37), urinary endothelin/endothelin-1 (ET/ET-1, WMD = 10.78, 95% CI: 8.18 to 13.39), levels of serum creatinine (SCr, WMD = 6.42, 95% CI: 1.83 to 11.01), blood urea nitrogen (BUN, SMD = 1.45, 95% CI: 0.19 to 2.71) and total cholesterol (TC, WMD = 0.84, 95% CI: 0.56 to 1.21, as well as in increasing high density lipoprotein-cholesterol (HDL-C, WMD = 0.17, 95% CI: 0.09 to 0.26), showing significant difference between groups. However, the effects of SF were insignificantly different to those of control in reducing fasting blood glucose (FBG, WMD = 0.17, 95% CI: -0.03 to 0.37) and triglyceride (TG, SMD = -0.13, 95% CI -0.49 to 0.23).
CONCLUSIONSAt present the evidences show that SF is superior to the conventional treatment in reducing UAER, ET, SCr, BUN, TC and increasing HDL-C, but there is no evidence to show that SF is superior in reducing FBG and TG. However, the evidence is not strong enough due to the low quality of included literature. More large-scale, multi-center, randomized trials are needed to confirm the efficacy and safety of SF in treating diabetic nephropathy.
Coumaric Acids ; therapeutic use ; Diabetic Nephropathies ; drug therapy ; Humans ; Phytotherapy
3.Preparation and properties of sustained-release pellets of active components from Ligusticun chuanxiong.
Yanlong HONG ; Xiao LING ; Yi FENG ; Desheng XU ; Lihua CHEN
China Journal of Chinese Materia Medica 2011;36(4):439-442
OBJECTIVETo prepare sustained-release pellets of active components from Ligusticun chuanxiong by coating in the fluid-bed.
METHODThe fast-release pellets were prepared by the extrusion-spheronization process. The coating formulation was optimized with the release of ferulic acid as criteria. The micromeritic properties and drug dissolution behaviors of the prepared pellets were evaluated.
RESULTThe pellets presented perfect sphericity. The obviously sustained-release effects were shown in 12 h. The coating weight gain was the major factor impacting drug release.
CONCLUSIONThe coating pellets show the obviously sustained-release effects.
Coumaric Acids ; analysis ; Delayed-Action Preparations ; Ligusticum ; chemistry ; Technology, Pharmaceutical
4.Characterization of highly active tyrosine ammonia lyase and its application in biosynthesis of p-coumaric acid.
Yawen HUANG ; Xiaolong JIANG ; Wujiu CHEN ; Guimin ZHANG ; Qinhong WANG
Chinese Journal of Biotechnology 2022;38(12):4553-4566
p-coumaric acid is one of the aromatic compounds that are widely used in food, cosmetics and medicine due to its properties of antibacterium, antioxidation and cardiovascular disease prevention. Tyrosine ammonia-lyase (TAL) catalyzes the deamination of tyrosine to p-coumaric acid. However, the lack of highly active and specific tyrosine ammonia lyase limits cost-effective microbial production of p-coumaric acid. In order to improve biosynthesis efficiency of p-coumaric acid, two tyrosine ammonia-lyases, namely Fc-TAL2 derived from Flavobacterium columnare and Fs-TAL derived from Flavobacterium suncheonense, were selected and characterized. The optimum temperature (55 ℃) and pH (9.5) for Fs-TAL and Fc-TAL2 are the same. Under optimal conditions, the specific enzyme activity of Fs-TAL and Fc-TAL2 were 82.47 U/mg and 13.27 U/mg, respectively. Structural simulation and alignment analysis showed that the orientation of the phenolic hydroxyl group of the conserved Y50 residue on the inner lid loop and its distance to the substrate were the main reasons accounting for the higher activity of Fs-TAL than that of Fc-TAL2. The higher activity and specificity of Fs-TAL were further confirmed via whole-cell catalysis using recombinant Escherichia coli, which could convert 10 g/L tyrosine into 6.2 g/L p-coumaric acid with a yield of 67.9%. This study provides alternative tyrosine ammonia-lyases and may facilitate the microbial production of p-coumaric acid and its derivatives.
Ammonia-Lyases/chemistry*
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Coumaric Acids
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Escherichia coli/genetics*
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Tyrosine
5.Effect of spring topdressing on yield, ferulic acid and total alkaloids of Ligusticum chuanxiong.
Yi ZHANG ; Qiao-jia FAN ; Shun-lin ZHENG ; Hong ZHOU ; Ji-chao YUAN ; Yu-ying MA ; Gui-hua JIANG
China Journal of Chinese Materia Medica 2008;33(16):1944-1947
A field trial was carried out to study the influence of different kinds of spring topdressing on growth, yield and quality of Ligusticum chuanxiong. The results showed that the spring topdressing had effects of improving root length, tiller numbers and plant height to some extent. At the same time the chlorophyll content and dry weight accumulation especially the dry weight of root increased significantly. It also showed that the yield increased and quality was improved significantly. The effect of different treatment with urea58.7 kg x hm(-2)(N 27 kg x hm(-2)) was the best and the treatment with N,P,K the second.
Alkaloids
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metabolism
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Coumaric Acids
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metabolism
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Fertilizers
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Ligusticum
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growth & development
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metabolism
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Seasons
6.Study on influence of buffer system on separate capacity of macroporous resin on ferulic liposome.
Bao-Yu CHEN ; Jing QIN ; Da-Wei CHEN ; Hai-Yang HU ; Ya-Shu YIN ; Kai-Liang YANG ; Yi-Fan LUO
China Journal of Chinese Materia Medica 2006;31(21):1780-1783
OBJECTIVETo study the effect of buffer on separate capacity of macroporous resin. To evaluate the quality of ferulic acid liposome and determine its entrapment efficiency.
METHODDifferent type of macroporous resin counterpoised by buffer system of Na2 HPO3-NaH2, PO3 was used to separate the free ferulic acid from the preparation and HPLC was used to determine the concentration of the ferulic acid to calculate the entrapment efficiency.
RESULTThis method had good linearity in the range of 0.56 - 2.8 g x mL(-1) (r = 0.999 6). The precision RSD was less than 1.1%. The adsorption effect of macroporous resin on liposome was reduced while it had no effect on the absorption ability of macroporous resin on the ferulic acid by the usage of buffer. The recovery of HPD450 resin on blank liposome was between 97.2% - 100.8%, while the average recovery is 98.1%.
CONCLUSIONBuffer system can enhance the separate ability of macroporous resin on liposome and free drug.
Adsorption ; Buffers ; Coumaric Acids ; administration & dosage ; analysis ; Drug Carriers ; Liposomes ; Quality Control ; Resins, Synthetic
7.Determination of ferulic acid in Fuan oral liquid by high-performance capillary electrophoresis.
Yi-Lei LI ; Ping ZHENG ; Yuan YAN ; Fang YANG
Journal of Southern Medical University 2009;29(3):551-552
OBJECTIVETo establish a quality control method of Fuan oral liquid.
METHODSHigh-performance capillary electrophoresis (HPCE) was used to determine the content of feruild acid in Herba taraxaci, and thin-layer chromatography (TLC) was performed to identify Herba taraxaci and Bupleurum chinense. The condition of HPCE was optimized with a fuse silica capillary tube (70 microm x 60 cm) and 20 mmol/L sodium tetraborate buffer (pH9.18) at a constant voltage of 12 kV and temperature at 25 degrees celsius;, with the detection wavelength at 313 nm.
RESULTSClear spots were displayed on TLC. The calibration curve was linear in the range of 8-40 microg/ml for ferulic acid (Y=360.5-207.4, r=0.9997, n=5). The average recovery rate exceeded 95% with a RSD<3% (n=3).
CONCLUSIONThis method is simple and specific with a good reproducibility for quality control of Fuan oral liquid.
Coumaric Acids ; analysis ; Drugs, Chinese Herbal ; chemistry ; Electrophoresis, Capillary ; methods ; Quality Control
8.Preparation of ferulic acid, senkyunolide I and senkyunolide H from Ligusticum chuanxiong by preparative HPLC.
Yao-Kun XIONG ; Shuang LIANG ; Yan-Long HONG ; Xiu-Juan YANG ; Lan SHEN ; Yan DU ; Yi FENG
China Journal of Chinese Materia Medica 2013;38(12):1947-1950
Preparative HPLC was used to prepare ferulic acid, senkyunolide I and senkyunolide H from Ligusticum chuanxiong. The separation was conducted on a Shim-Pack Prep-ODS (20.0 mm x 250 mm, 5 microm) column with the mobile phase of methanol-0.2% glacial acetic acid (50:50)at the flow rate of 5 mL x min(-1). The detection wavelength was 278 nm, and the purity of each compound was detected by HPLC analysis. Spectral data analyses including UV, ESI-MS and NMR were used to identify their structures. This method is simple, fast, which is suitable for preparation of standard reference of ferulic acid, senkyunolide I and senkyunolide H from L. chuanxiong and can meet the requirement of new drug research and development.
Benzofurans
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chemistry
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isolation & purification
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Chromatography, High Pressure Liquid
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methods
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Coumaric Acids
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chemistry
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isolation & purification
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Ligusticum
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chemistry
9.Determination of free ferulic acid and total ferulic acid in Chuanxiong by high-performance liquid chromatography for quality assessment.
Guanghua LV ; Shiqiong CHENG ; Kelvin CHAN ; Kelvin Sy LEUNG ; Zhongzhen ZHAO
China Journal of Chinese Materia Medica 2010;35(2):194-198
Ferulic acid (FA) is one of the main bioactive compounds in Chuanxiong (CX), the dried rhizome of Ligusticum chuanxiong, but its amount in this herb is difficult to determine accurately. An accurate quantificational method was developed to investigate on the available amount of FA (free FA and total FA). Herbal samples were extracted in methanol-formic acid (95:5) and methanol-0.24 mol x L(-1) sodium hydrogen carbonate in water (95:5), respectively and then quantitatively analyzed by HPLC method. Thirty three CX samples were quantified on free and total FA. Total FA was found more abundant than free FA with an average ratio of 2.38 (n = 32) in the range of 1.03- 4.98 in 32 CX herbs, and a highest ratio of 19.6 was estimated in a rhizome seedling. Results showed that total FA content would be a better marker for the quality assessment of CX herbs. Fifteen CX typical samples were collected from the trueborn cultivating areas in Sichuan province of China. The amount of total FA in these herbs was estimated to be 1.42 mg x g(-1) (n = 15). The proposed limit of total FA in CX samples should not less than 1.25 mg x g(-1) calculated on the dried basis. It was also found that the level of total FA was related to the quality, processing method and store duration of CX samples.
Chromatography, High Pressure Liquid
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methods
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Coumaric Acids
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analysis
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Drugs, Chinese Herbal
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analysis
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Ligusticum
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chemistry
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Quality Control
10.Researches on relationships between main active ingredients and physical and chemical parameters of dangguibuxue decoction.
Jiahua MA ; Xia LI ; Mingling ZHANG ; Nan LI ; Qin ZHENG ; Ming YANG
China Journal of Chinese Materia Medica 2010;35(15):1953-1956
OBJECTIVETo study relationships between the main active ingredients and physical and chemical parameters of dangguibuxue decoction, and to explore the feasibility of characterization the quality of decoction by physical and chemical parameters.
METHODSolutions of ferulic acid and astragaloside with different concentrations were prepared, of which physical and chemical parameters such as surface tension, pH value and conductivity, etc were determined at 25 degrees C. And polynomial regression analysis was used to analyze these data.
RESULTWith the increase of concentration of ferulic acid, pH value was decreased and conductivity was increased. And surface tension was decreased in the aqueous solution of astragaloside. In the combined solution of ferulic acid and astragaloside, ferulic acid was the main influencing pH value and conductivity, while astragaloside was the main factor influencing surface tension.
CONCLUSIONOn one hand, astragaloside concentration has a significant correlation with surface tension, but almost no correlation with pH value and conductivity. On the other hand, ferulic acid concentration has a strong correlation with pH value and conductivity, but almost no correlation with surface tension. In addition, each parameter has strong anti-interference ability and almost didn't change with the addition of another material. These preliminary results suggested that such characteristic parameters could be used to characterize the decoction quality.
Chemical Phenomena ; Coumaric Acids ; analysis ; Drugs, Chinese Herbal ; chemistry ; Surface Tension