This study was performed to observe the sequential charge of the morpholcgic characteristics in experimentally induced herpes simplex keratitis. Duration and morphology of corneal lesion following infection of rabbit cornea with the Kos strain of HSV-I were followed by a daily slit-lamp examination. Three types of virus inoculation methods were used such as scratching, deepithelialization, and intrastromal injection. Herpetic corneal lesions appeared 24 hours after inoculation with punctate and dendritic figures. They persisted up to 14 or 15 days. The characteristic finding in punctate herpetic keratitis was grouped, round-shaped, punctate lesion. When scratching method was emplyed, the most remarkable finding was the discontinuity of the lesion occurred along the scratching wound at relatively regular intervals. There was no difference in lesional morphology and duration between three inoculation methods.
Cornea ; Keratitis* ; Keratitis, Herpetic ; Wounds and Injuries

The effects of topical sodium-hyaluronan on the stromal and endothelial healing were examined in the repair process of experimental corneal alkali wounds. Corneal alkali wounds were produced in one eye of each rabbit by applying a 5.5 mm round filter paper soaked in 1N NaOH onto the central cornea for 60 seconds. Then the eyes were treated topically with either 1% Na-HA(the treatment group)or a phosphate buffered saline(PBS)(the con-trol group)4 times per day for 3 weeks. Endothelial wound morphometry was performed after alizarin red and trypan blue staining. The stromal healing was assessed by counting polymorphonuclear leukocytes(PMNs)and keratocytes in the central and marginal wounds areas. The stroma treated with Na-HA had less PMNs than that of the control group during the early healing period. The defect area of the endothelium was significantly smaller in the Na-HA treated group than in the control corneas. The present findings indicate that topically applied 1%Na-HA affects stromal and endothelial healing during the early repair process after corneal alkali wounds.
Alkalies* ; Cornea ; Endothelium ; Trypan Blue ; Wounds and Injuries*

The effects of topical sodium-hyaluronan (Na-HA)on the epithelial healing and aqueous compositions were examined in the repair process of experimen-tal corneal alkali wounds.Corneal alkali wounds were produced in one eye of each rabbit by applying a 5.5 mmround filter paper soaked in 1N NaOH onto the central cornea for 60 seconds.Then the eyes were treated topically with either 1%Na-HA (the treatment group)or a phosphate buffered saline (PBS) (the control group)4 times per day.The epithelial healing was assessed using a quantitative method and the changes of the concentration of ascorbic acid and glucose in aqueous were evaluated.The initial epithelial healing rate was similar between 1%Na-HA treated and control groups and the later healing was enhanced in the treated group but statistically insignificant.The concentrations of ascorbic acid and glucose in aqueous humor are not statistically different in the early phase, but ascorbic acid concentration in later healing phase was higher in the treated group when compared with the control group.The present findings in dicate that topical-ly applied 1%Na-HA affects late epithelial healing and ascorbic acid concentration in aqueous humor during the repair process after corneal alkali wounds.
Alkalies* ; Aqueous Humor ; Ascorbic Acid ; Cornea ; Epithelium ; Glucose ; Wounds and Injuries*

PURPOSE: This study analyzed the efficacy of Scion Image(R)program, the measurement of area of cornea lesion in the process of corneal diseases. METHODS: Photographs were processed digitally from three corneal wound lesions, and the Adobe Photoshop(R) and Scion Image(R) were used to calculate the area. These measured automatically the area of the damaged corneal lesion in numerical values, allowing precise calculation of the ratio of the wounded area to the entire cornea. RESULTS: In the first case of corneal abrasion by contact lenses, we were able to measure the ratio of the lesion, improving from 45% to 9% after two days of treatment. In the second case of the typical corneal ulcer, the infected area was 23.6% of the cornea before treatment, and decreased to 14.5% after treatment. In the third case of the ring shaped corneal ulcer, we were able to measure the lesion accurately using the Scion Image(R) CONCLUSIONS: The Scion Image(R)program may be used for the measurement of corneal surface lesion. It seemed to allow objective and accurate measurement.
Contact Lenses ; Cornea ; Corneal Diseases ; Corneal Ulcer ; Wounds and Injuries

We report a clinical ananlysis of children's perforating eye injuries which happened in middle western of Korea from Jan., 1988 to Apr., 1993. Total number of patients with perforating eye injuries was 40(40 eyes). The peak age group was 5-6 year of age. The cornea was the most common site of perforation. The most frequent cause of perforating eye injuries was knife and scissors(17.5%) and sharp pointed iron material(17.5%) and followed by glass(17.5%), wood(17.5%), pencil 7.5%), toy(5%), traffic accident(5%). According to this result obtained, the visual prognosis of perforating eye injuries in children is still poor. Special care should be taken to prevent such a severe eye trauma in children by every means.
Child ; Cornea ; Eye Injuries* ; Humans ; Iron ; Korea* ; Prognosis

The results of 8 cases of herpes simplex keratitis and one case of corneo-lenticular adhesion caused by explosion wound treated by cryogenic instrument (Amoils cryo unit) are presented. The cryoapplicator (2mm in diameter) were applied on the lesions 7-15 seconds. (Temperature: -50 degrees C ~ -70 degrees C). One of herpes simplex keratitis patients was suffered from redness of the lids following cryotherapy for 3 days.
Cornea* ; Cryotherapy ; Explosions ; Freezing* ; Humans ; Keratitis, Herpetic ; Wounds and Injuries

PURPOSE: To study the relationship between the concentration of ascorbic acid in the anterior chamber and the complications of an experimental alkali burn in rabbit corneas. METHODS: Standardized corneal alkali wounds were induced in both eyes of 33 albino rabbits by applying a 5.5 mm-sized round filter paper, soaked in 1N NaOH for 20 seconds. Then, the control group (22 eyes) was given Tears Natural II(R) (Alcon), treated group A (22 eyes) was given ascorbic acid (10%) for 2 hours, and treated group B (22 eyes) was given ascorbic acid (10%) for 6 hours. The concentrations of ascorbic acid in the anterior chamber were measured using a microplate assay immediately post-injury, and at 5 days, 15 days, and 30 days post-injury. During the experiment, clinical observation was performed to detect complications such as corneal ulcer or perforation. RESULTS: The incidence of ulceration was significantly lower between control group and treated groups A and B (p<0.05). The incidence of perforation was significantly lower in treated group A compared to the control group B (p<0.05). In addition, a higher concentration of ascorbic acid in the anterior chamber and a lower severity of corneal injury was significant (sigma=-0.302, P<0.05). CONCLUSIONS: Higher concentrations of ascorbic acid in the anterior chamber were associated with fewer complications in experimental alkali burns in rabbit corneas.
Alkalies ; Anterior Chamber* ; Ascorbic Acid* ; Burns ; Cornea ; Corneal Ulcer ; Incidence ; Rabbits ; Ulcer ; Wounds and Injuries

The linear scratching wound was made gently on the corneal epithelium of rabbit with 21 gauge needle under an operating microscope. Impression cytology was performed at 30 minutes, 1, 2, 3 and 4 hour and 1, 2, 3 and 4 day after 0.5% tetracaine drops under an operating microscope. The filter paper was stained with hematoxylin and eosin. At 30 minute post-scratching, a few polymorphonuclear leukocytes appeared on the scratched cornea at 3 eyes (30%). At 3 and 4 hour, numerous polymorphonuclear leukocytes with corneal epithelial cells appeared on the scratched conea. By 3 day, no inflammatory cells were shown on the filter paper in all eyes. These findings suggest that the polymorphonuclear leukocytes could infiltrate on the corneal lesion at 30 minute post-scratching and the inflammatory cells might act even on the minute corneal lesion such as corneal erosion.
Cornea ; Eosine Yellowish-(YS) ; Epithelial Cells ; Epithelium, Corneal ; Hematoxylin ; Needles ; Neutrophils* ; Tetracaine ; Wounds and Injuries*

An extracapsular lens extra.ction usually provides an excellent functional result and leaves an eye whose anatomic integrity in some ways exceeds that seen after an intracapsular lens extraction. This technique has the advantage of a smaller incision and a shorter convalescence. In the prevention of certain complications such as operative vitreous loss and postoperative cystoid macular edema, well planned extracapsular lens extraction before surgery is followed by far fewer complications than the misfortune of a ruptured capsule of an immature cataract. Cataract extraction was performed as usual conventional method. Intracapsular lens extraction was performed in 21 eyes and extracapsular lens extraction in 15 eyes. Although the incidence of iris adhesion to the cornea or vitreous, iris peaking, vitreous adhesion to the posterior corneal wound and operative rupture of the anterior hyaloid membrane were higher than those of the intracapsular lens extraction, sorious complications were not resulted. Fundus fluorescein angiograms were taken in all patients within 6 months postoperatively. The incidence of cystoid macular edema in the extracapsular lens extraction (33%) was lower than that of the intracapsular lens extraction(43%).
Cataract Extraction ; Cataract* ; Convalescence ; Cornea ; Fluorescein ; Humans ; Incidence ; Iris ; Macular Edema ; Membranes ; Rupture ; Wounds and Injuries

Healing process and histologic findings induced by various suture materials and suture methods were studied in this experiment. Albino rabbits were used and these rabbits were anesthesized by intravenous pentothal sodium injection(15 mg/kg) and 2 % lidocaine retrobulbar injection. The eye ball was luxated by bridle sutures on the sup. and info rectus muscles and the cornea was incised first with 15 Bard-Parker knife and the wound was extended with corneoscleral scissors 5 mm in length about 1 mm apart from the limbus at 12 and 9 o/c positions. Two interrupted penetrating and shndard sutures with 8-0 catgut were made at 12 and 9 o/c position respectively on the Rt. eye. Two interrupted standard sutures were made at 9 o/c position with 8-0 catgut suture and 2 interrupted standard sutered were made at 12 o/c position with 6-0 black silk suture on the Lt. eye. These rabbits were sacrificed at interval of 1, 2, and 3 weeks by overdose of pentothal sodium and eyes were immediately enucleated and fixed in 10 % formalin and stained with Hematoxylin and Eosin. The specimens were observed under light microscope. The differences in healing process and tissue reaction to catgut were not significant between the penetrating and standard suture group. The tissue reaction was milder in silk group and vascularization near the wound was found in catgut group.
Catgut ; Cornea ; Eosine Yellowish-(YS) ; Formaldehyde ; Hematoxylin ; Lidocaine ; Muscles ; Rabbits ; Silk ; Sodium ; Sutures* ; Thiopental ; Wounds and Injuries