1.Anti-complementary Activities of Exo- and Endo-biopolymer Produced by Submerged Mycelial Culture of Eight Different Mushrooms.
Byung Keun YANG ; Young Ah GU ; Yong Tae JEONG ; Chi Hyun SONG
Mycobiology 2007;35(3):145-149
The Elfvingia applanata (EA), Hericium erinaceum (HE),Grifola frondosa (GF), Pholiota nameko (PN), Pleurotus eryngii (PE), Trametes suaveolens (TS), Fomes fomentarius (FF), and Inonotus obliquus (IO) could produce the endo- (EN) and exo-biopolymer (EX) in submerged culture. The highest anti-complementary activity of the EN was exhibited by PN (49.1%), followed by HE (38.6%), TS (37.0%),and FF (33.0%),whereas the high activity of the EX was found with GF (59.8%),followed by HE (36.3%),TS (30.8%),and IO (28.8%). The EN of P. nameko (EN-PN) and EX of G. frondosa (EX-GF) were found to contain 78.6% and 41.2% carbohydrates, while 21.4% and 58.8% protein, respectively. The sugar and amino acid compositions of EN-PN and EX-GF were also analyzed in detail.
Agaricales*
;
Carbohydrates
;
Coriolaceae
;
Pholiota
;
Pleurotus
;
Trametes
2.Fomitoside K, a New Lanostane Triterpene Glycoside from the Fruiting Body of Fomitopsis nigra.
In Kyoung LEE ; Jin Young JUNG ; Ji Hee YEOM ; Dae Won KI ; Myeong Seok LEE ; Woon Hyung YEO ; Bong Sik YUN
Mycobiology 2012;40(1):76-78
In an effort to identify the chemical constituents of fruiting bodies of Fomitopsis pinicola, a new lanostane triterpene glycoside, designated as fomitoside K, has been isolated from its methanolic extract. Its chemical structure was assigned on the basis of various spectroscopic studies.
Coriolaceae
;
Fruit
;
Methanol
3.Checklist of Hymenomycetes (Aphyllophorales s.l.) and Heterobasidiomycetes in Israel.
Daniel TURA ; Ivan V ZMITROVICH ; Solomon P WASSER ; Eviatar NEVO
Mycobiology 2010;38(4):256-273
A checklist is presented concerning the species composition of Hymenomycetes (Aphyllophorales s.l.) and Heterobasidiomycetes in Israel based on data of previous studies and field sample collections. In total, 242 species are presented, of which five are new records for the Israeli mycobiota, namely Australohydnum dregeanum, Ceriporiopsis consobrina, C. resinascens, Fibroporia vaillantii, and Postia inocybe. The distribution and habitat of each species in Israel are also summarized. This checklist will serve as valuable data for future species diversity studies of these fungi in Israel.
Checklist
;
Coriolaceae
;
Ecosystem
;
Fungi
;
Israel
4.Degradation of Three Aromatic Dyes by White Rot Fungi and the Production of Ligninolytic Enzymes.
Chandana JAYASINGHE ; Ahmed IMTIAJ ; Geon Woo LEE ; Kyung Hoan IM ; Hyun HUR ; Min Woong LEE ; Hee Sun YANG ; Tae Soo LEE
Mycobiology 2008;36(2):114-120
This study was conducted to evaluate the degradation of aromatic dyes and the production of ligninolytic enzymes by 10 white rot fungi. The results of this study revealed that Pycnoporus cinnabarinus, Pleurotus pulmonarius, Ganoderma lucidum, Trametes suaveolens, Stereum ostrea and Fomes fomentarius have the ability to efficiently degrade congo red on solid media. However, malachite green inhibited the mycelial growth of these organisms. Therefore, they did not effectively decolorize malachite green on solid media. However, P. cinnabarinus and P. pulmonarius were able to effectively decolorize malachite green on solid media. T. suaveolens and F. rosea decolorized methylene blue more effectively than any of the other fungi evaluated in this study. In liquid culture, G. lucidum, P. cinnabarinus, Naematoloma fasciculare and Pycnoporus coccineus were found to have a greater ability to decolorize congo red. In addition, P. cinnabarinus, G. lucidum and T. suaveolens decolorized methylene blue in liquid media more effectively than any of the other organisms evaluated in this study. Only F. fomentarius was able to decolorize malachite green in liquid media, and its ability to do so was limited. To investigate the production of ligninolytic enzymes in media containing aromatic compounds, fungi were cultured in naphthalene supplemented liquid media. P. coccineus, Coriolus versicolor and P. cinnabarinus were found to produce a large amount of laccase when grown in medium that contained napthalene.
Coloring Agents
;
Congo Red
;
Coriolaceae
;
Fungi
;
Humans
;
Laccase
;
Methylene Blue
;
Naphthalenes
;
Ostrea
;
Pleurotus
;
Pycnoporus
;
Reishi
;
Rosaniline Dyes
;
Trametes
5.Diversity of Wood-Inhabiting Polyporoid and Corticioid Fungi in Odaesan National Park, Korea.
Yeongseon JANG ; Seokyoon JANG ; Jaejung LEE ; Hanbyul LEE ; Young Woon LIM ; Changmu KIM ; Jae Jin KIM
Mycobiology 2016;44(4):217-236
Polyporoid and corticioid fungi are among the most important wood-decay fungi. Not only do they contribute to nutrient cycling by decomposing wood debris, but they are also valuable sources for natural products. Polyporoid and corticioid wood-inhabiting fungi were investigated in Odaesan National Park. Fruit bodies were collected and identified based on morphological and molecular analyses using 28S and internal transcribed spacer regions of DNA sequences. As a result, a total of 149 species, 69 genera, 22 families, and 11 orders were recognized. Half (74 species) of the species were polypores, and the other half (75 species) were corticioid fungi. Most of the species belonged to Polyporales (92 species) followed by Hymenochaetales (33 species) and Russulales (11 species). At the genus level, a high number of species was observed from Steccherinum, Hyphodontia, Phanerochaete, Postia, and Trametes. Concerning distribution, almost all the species could be found below 1,000 m, and only 20% of the species were observed from above 1,000 m. Stereum subtomentosum, Trametes versicolor, T. hirsuta, T. pubescens, Bjerkandera adusta, and Ganoderma applanatum had wide distribution areas. Deciduous wood was the preferred substrate for the collected species. Sixty-three species were new to this region, and 21 species were new to Korea, of which 17 species were described and illustrated.
Base Sequence
;
Biological Products
;
Classification
;
Coriolaceae
;
Fruit
;
Fungi*
;
Ganoderma
;
Humans
;
Korea*
;
Parks, Recreational*
;
Phanerochaete
;
Polyporales
;
Trametes
;
Wood
6.First Report of Ceriporiopsis resinascens (Phanerochaetaceae, Basidiomycota) in Korea.
Jin Sung LEE ; Eun Ju WOO ; Young Woon LIM
Mycobiology 2009;37(4):305-307
An unrecorded Ceriporiopsis species was collected at Mt. Gariwang, Gangwon Province, in 2008. Based on morphological characteristics, such as a fully resupinate basidiocarp, a reddish white to pinkish poroid hymenophore and a monomitic hyphal system with clamp connections, the species was identified as Ceriporiopsis resinascens. This is the first report of Ceriporiopsis resinascens in Korea. We confirmed the identity of the species as Ceriporiopsis resinascens based on ITS sequence analysis.
Coriolaceae
;
Fruiting Bodies, Fungal
;
Humans
;
Korea
;
Sequence Analysis
7.Electromicroscopic Characterization about Vibrio alginolyticus Phage Isolated from Marine Products.
Jin Woo JU ; Seong A JU ; Moon Soo HEO ; So Gyem YOON ; Joo An OK ; Byoung Gon MOON
Journal of the Korean Society for Microbiology 1997;32(1):91-101
The study of bacteriophage began by F.W. Twort in 1915 and the lytic cycle recognized by d'Herellel in 1917. It repeated about the marine bacteriophage containing Vibrio phage by Smith, Spencer and Ju. Authors isolated 2 virulent phages for the pathogenic V. alginolyticus from marine products. These 2 phages were examined their ultrastructure & host-infection by elecron microscopy and in vivo test using skin of rats. V. alginolyticus phages(VAPs) fomed plaques about 0.5 - 0.9mm in diameter and bands 50 - 60% in sucrose density gradient. VAP had 50 - 120nm tail and 40 - 90nm head in diameter. In vivo test, using rat skin, as well as in vitro test VAP had the activity to V. alginolyticus isolated.
Animals
;
Bacteriophages*
;
Coriolaceae
;
Head
;
Microscopy
;
Rats
;
Skin
;
Sucrose
;
Tail
;
Vibrio alginolyticus*
;
Vibrio*
8.Characterization About Vibrio alginolyticus Phage Isolated from Marine Products.
Journal of the Korean Society for Microbiology 1997;32(1):81-90
Two phages for the pathogenic V. alginolyticus were isolated from marine products. These 2 phages were examined temperature stability, pH stability, inactivation by UV irradiation, damage on restriction system of host cell, antibody production, structure protein analysis and western blotting assay. V. alginolyticus phages(VAPs) fomed plaques about 0.5 - 0.9mm in diameter and bands 50 - 60% in sucrose density gradient, VAPs were stable below 65'C, pH 5 - 10 and mostly inactivation by UV irradiation for 120sec. Latend period was 15 - 20 min. and burst size was 1.3 - 1.4 * 10 PFU/cell. Restriction system of V. alginolyticus isolated was mostly inactivated by 45C, 20min. heating. VAPs had 14 specific structural proteins and 5 proteins related to antibody production.
Antibody Formation
;
Bacteriophages*
;
Blotting, Western
;
Coriolaceae
;
Heating
;
Hot Temperature
;
Hydrogen-Ion Concentration
;
Sucrose
;
Vibrio alginolyticus*
;
Vibrio*
9.Enhancement of beta-Glucosidase Activity from a Brown Rot Fungus Fomitopsis pinicola KCTC 6208 by Medium Optimization.
Ah Reum PARK ; Jeong Hoon PARK ; Hye Jin AHN ; Ji Yeon JANG ; Byung Jo YU ; Byung Hwan UM ; Jeong Jun YOON
Mycobiology 2015;43(1):57-62
beta-Glucosidase, which hydrolyzes cellobiose into two glucoses, plays an important role in the process of saccharification of the lignocellulosic biomass. In this study, we optimized the activity of beta-glucosidase of brown-rot fungus Fomitopsis pinicola KCTC 6208 using the response surface methodology (RSM) with various concentrations of glucose, yeast extract and ascorbic acid, which are the most significant nutrients for activity of beta-glucosidase. The highest activity of beta-glucosidase was achieved 3.02% of glucose, 4.35% of yeast extract, and 7.41% ascorbic acid where ascorbic acid was most effective. The maximum activity of beta-glucosidase predicted by the RSM was 15.34 U/mg, which was similar to the experimental value 14.90 U/mg at the 16th day of incubation. This optimized activity of beta-glucosidase was 23.6 times higher than the preliminary activity value, 0.63 U/mg, and was also much higher than previous values reported in other fungi strains. Therefore, a simplified medium supplemented with a cheap vitamin source, such as ascorbic acid, could be a cost effective mean of increasing beta-glucosidase activity.
Ascorbic Acid
;
beta-Glucosidase*
;
Biomass
;
Cellobiose
;
Coriolaceae*
;
Fungi*
;
Glucose
;
Vitamins
;
Yeasts
10.Hypoglycemic Effects of Exo-biopolymers Produced by Five Different Medicinal Mushrooms in STZ-induced Diabetic Rats.
Byung Keun YANG ; Guk Nam KIM ; Yong Tae JEONG ; Hun JEONG ; Pradeep MEHTA ; Chi Hyun SONG
Mycobiology 2008;36(1):45-49
Hypoglycemic effects of exo-biopolymers (EBP) produced by submerged mycelial cultures of Coriolus versicolor, Cordyceps sinensis, Paecilomyces japonica, Armillariella mellea, and Fomes fomentarius were investigated in streptozotocin (STZ)-induced diabetic rats. The rats from each experimental group were orally administered with EBPs (100 mg/kg BW) daily for 2 weeks. Though the hypoglycemic effect was achieved in all the cases, however, C. versicolor EBP proved as the most potent one. The administration of the C. versicolor EBP substantially reduced (29.9%) the plasma glucose level as compared to the saline administered group (control). It also reduced the plasma total cholesterol (TC), triglyceride (TG), aspartate aminotransferase (AST) and, alanine aminotransferase (ALT) levels by 9.22, 23.83, 16.93, and 27.31%, respectively. The sugar and amino acid compositions of this EBP were also analyzed in detail.
Agaricales
;
Alanine Transaminase
;
Animals
;
Aspartate Aminotransferases
;
Cholesterol
;
Cordyceps
;
Coriolaceae
;
Glucose
;
Hypoglycemic Agents
;
Paecilomyces
;
Plasma
;
Rats
;
Streptozocin