1.The Effect of Strychnine on Membrane Properties of Spinal Motoneurons in the Cat.
Kyu Chang LEE ; Manfred R KLEE ; Hun Jae LEE
Yonsei Medical Journal 1975;16(2):1-28
Strychnine (Stry.) has been used, as an instrument for studies of experimental epilepsy, though its precise mode of action has remained obscure. One mechanism of action was partially clarified in 1954 ,by the demonstration that subconvulsive doses of Stry. reduce the amplitude of inhibitory postsynaptic potentials (IPSPs) in the cat's spinal motoneurons (MN). Because of the rapid onset of its action and the absence of effects upon monosynaptic excitatory postsynaptic potentials (EPSPs), it was proposed that Stry. competed with some unidentified transmitter for inhibitory receptor sites on the postsynaptic membrane. Electrophoresis of Stry. is known to block the inhibitory effects of glycine, a likely candidate as an inhibitory transmitter on MN in the cat spinal cord. A Stry. resistant inhibition seems to exist not only in the higher portion of the CNS, but also for the spinal MN. Gamma amino butyric acid (GABA) is a candidate for this synaptic transmitter. In Nembutal anesthetized cat, intracellular recording of spinal MN was performed during Stry. induced seizure. To conclude, it can be said that there were no consistant changes in the MN action potential which would reflect an action of Stry. upon MN's membrane properties important to seizure generation. It is still to be resolved whether the increase in polysynaptic EPSP amplitude is due to a Stry. effect upon the membrane properties of excitatory interneurons or to an effect only upon the inhibitory as well as the EPSPs.
Action Potentials/drug effects*
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Animal
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Cats
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Convulsions/chemically induced
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Female
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Male
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Membrane Potentials/drug effects*
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Motor Neurons/drug effects*
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Spinal Cord/drug effects*
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Strychnine/pharmacology*
2.Effect of beta-Adrenergic Blockers on Experimentally-induced Convulsion and Narcosis.
Sook HUH ; Kyung Hwan KIM ; Sa Suk HONG
Yonsei Medical Journal 1978;19(1):25-31
Effects of beta-adrenergic blockers and related agents were investigated on experimental convulsions of chicks induced with strychnine, pentylenetetrazol or electroshock and on thiopental sleeping time of rabbits. Convulsions of chicks due to strychnine were significantly inhibited by all beta-adrenergic blockers except dichloroisopreterenol. Propranolol inhibited electroshock convulsion as well, but none of the blockers inhibited pentylenetetrazol convulsion. Furthermore, the mortality of chicks due to large dose of pentylenetetrazol was greatly increased by treatment of beta-adrenergic blockers. Pindolol alone showed diazepam-like anticonvulsive effect against low doses of pentylenetetrazol. Pretreatment with beta-adrenergic blockers caused a marked increase in thiopental sleeping time in rabbits. Prolongation of thiopental sleep due to propranolol was abolished by premedication of animals with reserpine or tranylcypromine. Thiopental sleeping time was prolonged by Zizyphus extract, though less effective than beta-adrenergic blockers. It is felt that the anticonvulsive or sleep enhancing effect of beta-adrenergic blocking agents has an intimate relationship with endogenous adrenergic amines and the receptors.
Adrenergic beta-Antagonists/pharmacology*
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Anesthesia
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Animal
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Anticonvulsants*
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Blood Pressure/drug effects
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Chickens
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Convulsions/chemically induced
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Heart Rate/drug effects
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Male
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Propranolol/pharmacology*
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Rabbits
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Strychnine/antagonists & inhibitors
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Thiopental