1.Molecular Epidemiology of Viral Conjunctivitis in the Southern Region of South Korea, 2012–2016.
Duck Woong PARK ; Min Ji KIM ; Kwang gon KIM ; Sun Ju CHO ; Hye Jung PARK ; Ji Hyun SHIN ; Yi Deun HA ; Mi Hee SEO ; Jang Hoon KIM ; Yeon LEE ; Myoung Doo PARK ; Hi Mo YOON ; Eun Sun KIM ; Young Jin HONG ; Hyeyoung KEE ; Jae Keun CHUNG
Journal of Bacteriology and Virology 2018;48(2):59-66
Epidemic keratoconjunctivitis (EKC) and acute hemorrhagic conjunctivitis (AHC) are common diseases caused by human adenoviruses (HAdV) and enteroviruses, respectively, in South Korea. However, there are limited studies on the molecular epidemiology of viral conjunctivitis in South Korea. The main objective of this study was to characterize the genotypes of adenoviruses and enteroviruses causing viral conjunctivitis in the southwest region of South Korea. We collected conjunctival swabs from 492 patients with suspected cases of viral conjunctivitis from 6 ophthalmic hospitals in Gwangju Metropolitan City, in South Korea, between 2012 and 2016. Of the 492 samples tested, HAdVs and enteroviruses were detected in 249 samples (50.6%) and 19 samples (3.9%), respectively. The genotype analysis detected HAdV-8 in 183 samples (73.5%), HAdV-37 in 14 samples (5.6%), and HAdV-3, and HAdV-4 in 9 samples (3.6%) each. We detected coxsackievirus A24 (CVA24) and coxsackievirus B1 (CVB1) in 8 samples (42.0%) and 4 samples (21.0%), respectively. We also reported for the first time HAdV-56-infected cases of EKC in South Korea. Furthermore, we found three cases of coinfection with HAdV and enterovirus genotypes in our samples. HAdV-8 and CVA24, the main causes of EKC and AHC, respectively, worldwide, were also found to be the predominant genotypes in our study.
Adenoviridae
;
Adenoviruses, Human
;
Coinfection
;
Conjunctivitis, Acute Hemorrhagic
;
Conjunctivitis, Viral*
;
Enterovirus
;
Genotype
;
Gwangju
;
Humans
;
Keratoconjunctivitis
;
Korea*
;
Molecular Epidemiology*
2.A Study for Viral Identification of Acute Viral Conjunctivitis.
Jae Chan KIM ; Hack Cheol KIM ; Nam Joo MOO ; Kyung Hwawn SHYN
Journal of the Korean Ophthalmological Society 1992;33(1):32-38
Acute viral conjunctivitis was known as an infectious disease affected by various viruses and characterized seasonal variation, prevalent especially in summer. In Korea, the clinic loepidemiologic study of AVC was not conclusive. Therefore, further research should be conducted to improve the primary health care and the application of diffrential diagnosis and its therapy. Clinical symptoms and signs of 131 patients who was confirmed viral conjunctivitis among 237 patients was evaluated at the Chung-Ang University Hospital in 1987, 1989 and 1990. Along with physical examination, specimens from the conjunctival swab were cultured and isolated, and a sereologic test-Neutralizing test for enterovirus 70, and complement fixation test for adenovirus-was performed. As a result, acute hemorrhagic conjunctivitis was relatively epidemic in 1987 and 1990 (67.7 and 47.1 %), and epidemic keratoconjunctivitis in 1989 (91.8%).
Communicable Diseases
;
Complement Fixation Tests
;
Conjunctivitis, Acute Hemorrhagic
;
Conjunctivitis, Viral*
;
Diagnosis
;
Enterovirus D, Human
;
Humans
;
Keratoconjunctivitis
;
Korea
;
Physical Examination
;
Primary Health Care
;
Seasons
3.Causes of epidemic keratoconjunctivitis and therapeutic measures.
Journal of the Korean Medical Association 2017;60(6):491-496
Epidemic keratoconjunctivitis is the most common type of infectious conjunctivitis, and is caused by adenoviruses. The mode of transmission is mainly through direct contact with ocular secretions. Epidemic keratoconjunctivitis is generally diagnosed based on a patient's clinical features, and additional measures, such as cell cultures, polymerase chain reaction, and rapid antigen detection tests, can further confirm the diagnosis. The most common symptoms include a foreign body sensation, tearing, and photophobia. The symptoms are usually expressed unilaterally in the initial phase, but gradually become bilateral. Frequently occurring complications include pseudomembrane formation and subepithelial infiltrates. Currently, no antiviral agent has been proven effective to alter the natural course of the disease, and treatment merely has a supportive role instead of a curative role. Therefore, preventive measures in medical offices and in the community are the most important methods of controlling the propagation of this disease.
Adenoviridae
;
Adenovirus Infections, Human
;
Cell Culture Techniques
;
Conjunctivitis
;
Conjunctivitis, Viral
;
Diagnosis
;
Foreign Bodies
;
Humans
;
Keratoconjunctivitis*
;
Photophobia
;
Polymerase Chain Reaction
;
Sensation
;
Tears
4.A Case of Monocular Gonococcal Conjunctivitis in an Adult Male.
You Hyun LEE ; Nam Hee RYOO ; Jong Hwa JUN
Keimyung Medical Journal 2018;37(2):101-105
Gonococcal conjunctivitis is rare in adults and, if not treated properly, can cause corneal perforation. Gonococcal conjunctivitis typically presents with a severe mucopurulent discharge, similar to that associated with viral conjunctivitis. Here, we describe a case of monocular gonococcal conjunctivitis, including its clinical characteristics and slit-lamp images, which was initially misdiagnosed as epidemic conjunctivitis. A 20-year-old man was referred to our hospital with no improvement in monocular infection and purulent ocular discharge after 2-wk treatment using antibiotic and 0.1% fluorometholone eye drops at the local ophthalmic clinic. Initially, 0.5% loteprednol eye drops were used since we suspected viral conjunctivitis. Following this treatment, conjunctival infection worsened and a yellow-white ocular discharge covered the conjunctiva and cornea surface. Additional history taking revealed that the patient had sexual contact with a prostitute 1 wk prior to symptom presentation and, after the encounter, he took antibiotics for genital discharge at the local urology clinic, but self-discontinued treatment. A Gram staining showed gram-negative diplococci and culture of collected ocular discharge from the palpebral conjunctiva revealed growth of Neisseria gonorrhoeae, confirming gonococcal conjunctivitis. Following this, the patient was systemically treated with 3rd generation cephalosporin antibiotics. After 3-d treatment, conjunctival infection and purulent ocular discharge had significantly improved. When clinical symptoms are aggravated following steroid eye drop treatment for suspected monocular viral conjunctivitis, gonococcal conjunctivitis must be considered as a differential diagnosis
Adult*
;
Anti-Bacterial Agents
;
Cephalosporins
;
Conjunctiva
;
Conjunctivitis*
;
Conjunctivitis, Viral
;
Cornea
;
Corneal Perforation
;
Diagnosis, Differential
;
Fluorometholone
;
Humans
;
Loteprednol Etabonate
;
Male*
;
Neisseria gonorrhoeae
;
Ophthalmic Solutions
;
Sex Workers
;
Urology
;
Young Adult
5.Delayed Epithelial Ingrowth Caused by Viral Conjunctivitis after LASIK.
Roo Min JUN ; Wool SUH ; Eung Kweon KIM
Yonsei Medical Journal 2008;49(4):662-665
To report a patient who presented with epithelial ingrowth caused by viral keratoconjunctivitis 3 months after LASIK surgery. A 41-year-old man presented with decreased visual acuity in the right eye, which had developed about 3 weeks before. He had undergone LASIK surgery 3 months prior without complications. Two months after the surgery, he was treated for viral conjunctivitis. During the treatment period, filamentary keratitis developed, and a therapeutic bandage contact lens was applied for 2 weeks. Upon presentation, examination revealed a corrected visual acuity of 20/100 and irregular epithelial sheets under the edematous flap. The flap was lifted, and the in-grown epithelium was removed. The flap was repositioned with double continuous 10-0 nylon sutures. Post-operatively, the patient developed a mild diffuse lamellar keratitis that resolved rapidly with topical corticosteroid treatment. At 2 months, the corrected visual acuity was 20/20 without interface opacities. As the patient showed no complications prior to viral conjunctivitis, we suspect that the viral infection caused edema of the corneal flap, which caused epithelial ingrowth under the flap. Patients who have viral conjunctivitis after LASIK surgery should be examined carefully and managed with consideration of flap complications.
Adult
;
Cell Proliferation
;
Conjunctivitis, Viral/*etiology/*pathology
;
Epithelial Cells/*pathology
;
Humans
;
Keratomileusis, Laser In Situ/*adverse effects
;
Male
6.Outbreak of acute hemorrhagic conjunctivitis in Beijing City in 2007 caused by coxsackievirus A24 variant: molecular identification and phylogenetic analysis.
Li-Xin DING ; Yong ZHANG ; Jie LI ; Xiang-Feng DOU ; Dong-Mei YAN ; Shuang-Li ZHU ; Hong-Qiu AN ; Wen-Bo XU
Chinese Journal of Virology 2009;25(4):251-256
In 2007, an outbreak of acute hemorrhagic conjunctivitis (AHC) occurred in Beijing. In order to identify the etiology of this outbreak, 57 eye conjunctival swabs were collected from 57 outpatient patients, and detected for adenovirus, human enterovirus 70 (HEV70) and Coxsackievirus A24 variant (CVA24v) genes by using RT-PCR or PCR methods. The results showed that 38 were positive for CVA24v, the positive rate was 66.7%, but none was positive for HEV70 and adenovirus, showing that this outbreak was caused by CVA24v. 9 viral isolates were obtained from 57 clinical specimens by using viral isolation method, and all were identified as CVA24v by molecular typing method. All 9 CVA24v isolates were performed by VP1 sequencing, the results showed that except for strain 0744/BJ/CHN/2007, the variability at nucleotide acid level and amino acid level among other 8 CVA24v were relatively low, and the homologies were more than 99.6% and 100.0%, respectively; the homologies of nucleotide acid and amino acid between strain 0744/BJ/CHN/2007 and other 8 CVA24v were 96.8%-97.2% and 99.7%, respectively. Phylogenetic analysis of 9 CVA24v revealed that they represented the Clade 4 and Clade 5 in Group I, showed that this outbreak was caused by at least 2 viral transmission chains. Comparing to 3C region of CVA24v frequently used before, VP1 region was considered as the most rigorous target for molecular epidemiology study of CVA24v. To enhance the research of sero-epidemiology and molecular epidemiology of CVA24v and to know the genetic characterizations and molecular evolution of CVA24v are most important to prevent and control the outbreaks of AHC in China.
China
;
epidemiology
;
Conjunctivitis, Acute Hemorrhagic
;
epidemiology
;
virology
;
Disease Outbreaks
;
Enterovirus C, Human
;
classification
;
genetics
;
isolation & purification
;
Humans
;
Molecular Epidemiology
;
Molecular Sequence Data
;
Phylogeny
;
Viral Proteins
;
genetics
7.Study on the pathological and molecular characteristics of AHC epidemic in Zhejiang Province in 2010.
Ju-Ying YAN ; Yin CHEN ; Zhen LI ; Li-Ming GONG ; Yi-Yu LU ; Yan-Jun ZHANG
Chinese Journal of Virology 2011;27(5):421-426
To identify and trace the pathogen of acute hemorrhagic conjunctivitis (AHC) epidemic in Zhejiang Province in 2010. Viral nucleic acid of Enterovirus (EV) and Coxsackievirus A24 variant (CA24v) were directly detected by real-time RT-PCR from the conjunctival swab collected from suspected patients. The virus was isolated from the swab samples using Hep-2 cell. The viral RNAs were extracted from the isolated viruses and followed by RT-PCR to amplify VP1 gene and 3C protease region(3C). The amplified fragments were sequenced and phylogenetic trees were also constructed. Eight out of 13 swab samples from suspected patients were both positive for EV and CA24v RNA (61.5%), 6 CA24v strains were isolated (46.2%). The complete VP1 genes of CA24v in 4 sequenced virus strains were 915 nt in length and the complete 3C genes were 549 nt in length. All VP1 and 3C genes were confirmed without any insertion or deletion. The identity of nucleotide and amino acid in 3C between the 2010 isolated strains and the prototype strain EH24/70 were 85.2%-85.8% and 96.2%-96.7%, and that between the 2010 Zhejiang strains and the Zhejiang,Yunnan and Guangdong CA24v strains isolated between 2007-2008 were 93.4%-93.8% and 96.7%-97.3%, respectively. The phylogenetic tree of 3C indicated that the isolated CA24v viruses of Zhejiang in 2010 located in the CA24v IV genotype cluster 4 (GIV-C4) and all the VP1 genes located in the human Enterovirus C (EV-C) CA24v. These findings indicated that AHC epidemic in Zhejiang Province in 2010 was caused by CA24v GIV-C4 viruses and they most likely evolved from CA24v viruses circulating locally in external environment from 2002.
Amino Acid Sequence
;
China
;
epidemiology
;
Conjunctivitis, Acute Hemorrhagic
;
epidemiology
;
virology
;
Coxsackievirus Infections
;
epidemiology
;
virology
;
Disease Outbreaks
;
Enterovirus
;
classification
;
genetics
;
isolation & purification
;
Enterovirus Infections
;
epidemiology
;
virology
;
Genes, Viral
;
genetics
;
Humans
;
Molecular Sequence Data
;
Phylogeny
;
RNA, Viral
;
genetics
;
Sequence Alignment
;
Sequence Homology
8.Molecular Identification and Phylogenetic Analyses of Coxsackievirus A24v Causing an Outbreak of Acute Hemorrhagic Conjunctivitis in Jiangxi, China, in 2010.
Dongmei YAN ; Ying XIONG ; Yang ZHANG ; Qiai YANG ; Shuxia ZHANG ; Tian GONG ; Tian ZHU ; Donavan WANG ; Hui ZHU ; Wenbo XU
Chinese Journal of Virology 2015;31(3):251-257
To identify the cause of an outbreak of acute hemorrhagic conjunctivitis (AHC) in Jiangxi (China) in 2010, 20 eye conjunctival swabs were first collected from AHC patients. Then, viruses were isola- ted and tested for human enterovirus 70, coxsackievirus A24 variant (CV-A24v) and adenovirus using the polymerase chain reaction. All CV-A24v isolates underwent sequencing of 3C and VP1 coding regions. Then, a phylogenetic tree was constructed for Jiangxi CV-A24v and worldwide CV-A24v based on,3C and VP1 regions, respectively. Ten out of 20 specimens were positive for CV-A24v, implying that the outbreak was caused by CV-A24v. The phylogenetic tree based on the 3C region showed that Jiangxi CV- A24v belonged to cluster 5 in genotype IV (GIV-C5) with strains isolated throughout the world after 2010, and were divided further into A and B lineages. Phylogenetic analyses of the VP1 region showed that all of the worldwide CV-A24v strains isolated after 2000 could be divided into five groups (1-5). Jiangxi CV-A24v was classified into group 5 and also divided further into A and B lineages upon analyses of the 3C region. These data suggested that CV-A24v causing AHC outbreaks in China in 2010 belonged to GIV-C3 and GIV-C5. At least two transmission lineages were circulated in Jiangxi in 2010. The classification of CV-A24v isolated after 2010 worldwide using the phylogenetic tree based on the VP1 region was almost consistent with that based on the 3C region and also had significant chronological clustering.
China
;
epidemiology
;
Conjunctivitis, Acute Hemorrhagic
;
epidemiology
;
virology
;
Coxsackievirus Infections
;
epidemiology
;
virology
;
Disease Outbreaks
;
Enterovirus C, Human
;
classification
;
genetics
;
isolation & purification
;
Genotype
;
Humans
;
Molecular Sequence Data
;
Phylogeny
;
Viral Proteins
;
genetics
9.Identification and genetic characterization of coxsackievirus A24 isolated from patients with acute hemorrhagic conjunctivitis in Shandong Province.
Jing YANG ; Yi LIN ; Hai-Yan WANG ; Ze-Xin TAO ; Yan LI ; Peng CHEN ; Yao-Wen PEI ; Feng JI ; Xiao-Juan LIN ; Xian-Jun WANG ; Yao LIU ; Ai-Qiang XU
Chinese Journal of Virology 2012;28(6):663-669
To identify the pathogen of acute hemorrhagic conjunctivitis (AHC) in Shandong Province in 2010, eye mucous swab samples were collected from 26 patients in Qingdao and Linyi City. Real time-PCR assays for EV70, CVA24 and Adenovirus were performed on these samples. The result showed 17 samples (65.39%) were CVA24 positive while all the samples for HEV70 and Adenovirus detection were negative, which implied that CVA24 was the causative pathogen of this outbreak. A total of 10 virus strains isolated on Hep-2 cells were identified as CVA24 through VP1 amplification and nucleotide sequence analysis. The nucleotide and amino acid homologies on VP1 region among these isolates were 99.3%-100.0% and 99.5%-100.0%, respectively, and the strains aggregated together to one clade in phylogenetic tree. These results showed that the CVA24 circulating in Qingdao and Linyi City belonged to one transmission chain. Shandong CVA24s segregated into 5 different clades, and great nucleotide divergence was observed be tween AHC isolates and others.
Amino Acid Sequence
;
China
;
epidemiology
;
Conjunctivitis, Acute Hemorrhagic
;
epidemiology
;
virology
;
Enterovirus C, Human
;
chemistry
;
classification
;
genetics
;
isolation & purification
;
Genetic Variation
;
Humans
;
Molecular Sequence Data
;
Phylogeny
;
Sequence Homology, Amino Acid
;
Viral Proteins
;
chemistry
;
genetics
10.Development of EKC after Eximer Laser Photorefractive Surgery and Subsequent Recurrence of EKC-like Keratitis.
Sung Joon PARK ; Yoon Soo JANG ; Tae Hyuk KOH ; Young A KWON ; Sang Wroul SONG
Korean Journal of Ophthalmology 2011;25(6):443-446
This research focuses on four cases of patients having undergone eximer laser photorefractive surgery who were diagnosed with adenoviral keratoconjunctivitis during the postoperative period and who later developed epidemic keratoconjunctivitis (EKC)-like keratitis. Two of the patients had undergone laser-assisted subepithelial keratectomy (LASEK), one had undergone laser in situ keratomileusis and one had photorefractive keratectomy. After the surgery adenoviral keratoconjunctivitis and recurrent late-developing EKC-like keratitis were observed in the patients. Recurrent late-developing EKC-like keratitis occurred in one of the patients, who had received LASEK as many as three times. The others had only one or two episodes.The corneal infiltrates of keratitis mainly occurred in the central cornea. Successful resolution of recurrent late-developing EKC-like keratitis was achieved through the use of topical steroids without sequelae and the final best-corrected visual acuity was as good as the base line. These keratitis infiltrates have been presumed to represent an immune response to the suspected adenoviral antigens deposited in corneal stroma during the primary adenoviral infection. Previous reports argued that patients with a history of adenoviral ketatoconjunctivitis were succeptible to adenoviral keratoconjunctivitis becoming reactivated; however, in our research, our patients had their first adenoviral infections after the eximer laser photorefractive surgery and reactivation was confirmed. We recommend that attention be paid to adenoviral infection after laser refractive operations, because these patients seem to have more frequent recurrences.
Adenovirus Infections, Human/diagnosis/drug therapy/*etiology
;
Adult
;
Conjunctivitis, Viral/diagnosis/drug therapy/*etiology
;
*Corneal Surgery, Laser
;
Eye Infections, Viral/diagnosis/drug therapy/*etiology
;
Female
;
Glucocorticoids/therapeutic use
;
Humans
;
Keratectomy, Subepithelial, Laser-Assisted
;
Keratitis/diagnosis/drug therapy/*etiology
;
Keratomileusis, Laser In Situ
;
Lasers, Excimer/therapeutic use
;
Photorefractive Keratectomy
;
*Postoperative Complications
;
Recurrence
;
Young Adult