Epidemic keratoconjunctivitis (EKC) and acute hemorrhagic conjunctivitis (AHC) are common diseases caused by human adenoviruses (HAdV) and enteroviruses, respectively, in South Korea. However, there are limited studies on the molecular epidemiology of viral conjunctivitis in South Korea. The main objective of this study was to characterize the genotypes of adenoviruses and enteroviruses causing viral conjunctivitis in the southwest region of South Korea. We collected conjunctival swabs from 492 patients with suspected cases of viral conjunctivitis from 6 ophthalmic hospitals in Gwangju Metropolitan City, in South Korea, between 2012 and 2016. Of the 492 samples tested, HAdVs and enteroviruses were detected in 249 samples (50.6%) and 19 samples (3.9%), respectively. The genotype analysis detected HAdV-8 in 183 samples (73.5%), HAdV-37 in 14 samples (5.6%), and HAdV-3, and HAdV-4 in 9 samples (3.6%) each. We detected coxsackievirus A24 (CVA24) and coxsackievirus B1 (CVB1) in 8 samples (42.0%) and 4 samples (21.0%), respectively. We also reported for the first time HAdV-56-infected cases of EKC in South Korea. Furthermore, we found three cases of coinfection with HAdV and enterovirus genotypes in our samples. HAdV-8 and CVA24, the main causes of EKC and AHC, respectively, worldwide, were also found to be the predominant genotypes in our study.
Adenoviridae ; Adenoviruses, Human ; Coinfection ; Conjunctivitis, Acute Hemorrhagic ; Conjunctivitis, Viral* ; Enterovirus ; Genotype ; Gwangju ; Humans ; Keratoconjunctivitis ; Korea* ; Molecular Epidemiology*

Acute viral conjunctivitis was known as an infectious disease affected by various viruses and characterized seasonal variation, prevalent especially in summer. In Korea, the clinic loepidemiologic study of AVC was not conclusive. Therefore, further research should be conducted to improve the primary health care and the application of diffrential diagnosis and its therapy. Clinical symptoms and signs of 131 patients who was confirmed viral conjunctivitis among 237 patients was evaluated at the Chung-Ang University Hospital in 1987, 1989 and 1990. Along with physical examination, specimens from the conjunctival swab were cultured and isolated, and a sereologic test-Neutralizing test for enterovirus 70, and complement fixation test for adenovirus-was performed. As a result, acute hemorrhagic conjunctivitis was relatively epidemic in 1987 and 1990 (67.7 and 47.1 %), and epidemic keratoconjunctivitis in 1989 (91.8%).
Communicable Diseases ; Complement Fixation Tests ; Conjunctivitis, Acute Hemorrhagic ; Conjunctivitis, Viral* ; Diagnosis ; Enterovirus D, Human ; Humans ; Keratoconjunctivitis ; Korea ; Physical Examination ; Primary Health Care ; Seasons

BACKGROUND: Olopatadine hydrochloride ophthalmic solutions are treated for allergic conjunctival diseases that are a selective histamine H1 receptor antagonist and an inhibitor of the release of mediators including histamine from the human mast cells. Substance P (SP) levels are increased in tears of patients with allergic conjunctivitis. However, little is known about the regulation of SP release by anti-allergic ophthalmic solutions. OBJECTIVE: We investigated that the effect of olopatadine hydrochloride ophthalmic solutions (olopatadine 0.1% and olopatadine 0.2%) on rat conjunctivitis models compared with other anti-allergic ophthalmic solutions. METHODS: Conjunctivitis was induced by subconjunctival injection of histamine or intravenous injection of ovalbumin in rats passively sensitized with anti-ovalbumin anti-serum. The releases of SP were determined in the conjunctiva and tears using rat antigen-induced conjunctivitis models. RESULTS: Olopatadine 0.1% and 0.2% significantly inhibited the increased conjunctival dye leaked in the histamine- or antigen-induced hyperpermeability. The inhibitory effects by olopatadine were more potent than by other tested anti-allergic ophthalmic solutions. Moreover, olopatadine significantly inhibited the release of SP from the conjunctiva. CONCLUSION: These results indicate that olopatadine ophthalmic solutions appear to exert additional SP release inhibition besides dual-action such as selective histamine H1 receptor antagonistic action and mast cell stabilization action.
Animals ; Conjunctiva ; Conjunctival Diseases ; Conjunctivitis ; Conjunctivitis, Allergic ; Histamine ; Humans ; Injections, Intravenous ; Mast Cells ; Olopatadine Hydrochloride ; Ophthalmic Solutions ; Ovalbumin ; Rats ; Receptors, Histamine H1 ; Substance P ; Tears

No abstract available.
Conjunctivitis, Acute Hemorrhagic* ; Korea*

No abstract available.
Conjunctivitis, Acute Hemorrhagic* ; Korea*

Cell Adhesion Molecule(CAM) is a cell surface glycoprotein that plays an important role in many inflammatory reaction. This is responsible for the migration and accumulation of different populations of leukocyte in inflamed tissues. To investigate the relevance of CAM expression to giant papillary conjunctivitis associated with type I and/or type IV hypersensitivity, the histology of conjunctival giant papillae from patients with papillary conjunctivitis was examined with light microscopy and using indirect immunofluorescent staining method with monoclonal antibodies against the ICAM-1, VCAM-1, and CD44. The infiltrates of the inflammatory cells such as eosinophil, basophil, plasma cell and lymphocyte were noted in conjunctival stroma by light microscopy. The ICAM-1, VCAM-1, and CD44 were expressed or upregulated in stroma and vascular wall by immunofluorescent method. These findings suggest that CAM may play a key role in the pathogenesis of giant papillary conjunctivitis. Further efforts to block or modulate the expression of CAMs may provide new therapeutic modalities in the treatment of conjunctival disease.
Antibodies, Monoclonal ; Basophils ; Cell Adhesion ; Conjunctival Diseases ; Conjunctivitis ; Conjunctivitis, Allergic* ; Eosinophils ; Humans ; Hypersensitivity, Delayed ; Intercellular Adhesion Molecule-1* ; Leukocytes ; Lymphocytes ; Membrane Glycoproteins ; Microscopy ; Plasma Cells ; Vascular Cell Adhesion Molecule-1*

PURPOSE: To evaluate tear eosinophil cationic protein (ECP) as a severity marker for atopic keratoconjunctivitis (AKC) and seasonal/perennial allergic conjunctivitis (SAC/PAC). METHODS: Tear ECP levels were measured by chemiluminescent immunometric assay in 7 eyes of 7 patients with AKC, 13 eyes of 13 patients with SAC/PAC, and 10 eyes of 10 healthy control subjects. All AKC and SAC/PAC patients underwent conjunctival injection and papillary formation grading. Tear ECP levels were investigated with reference to the clinical parameters of allergic conjunctivitis (papillary formation and conjunctival injection scoring). RESULTS: Tear ECP levels in patients with AKC were significantly higher than those in patients with SAC/PAC and in control subjects (p = 0.012 and p = 0.003, respectively). The number of patients with papillary formation scores of 2-3 was significantly higher in the AKC group than in the SAC/PAC group (p = 0.016). The number of patients with conjunctival injection scores of 2-3 did not significantly differ between the AKC and SAC/PAC groups (p = 0.128). All AKC patients obtained papillary formation scores of 2-3, and tear ECP levels in patients with conjunctival injection scores of 2-3 were significantly higher than in patients with scores of 0-1 in the AKC group (p < 0.001). In the SAC/PAC group, tear ECP levels in patients with papillary formation scores of 2-3 were significantly higher than in patients with scores of 0-1 (p = 0.046). CONCLUSIONS: This study suggests that tear ECP was a useful marker to diagnose and assess the severity of disease in patients with AKC as well as SAC/PAC. It would be useful to monitor therapeutic outcome in allergic conjunctivitis.
Conjunctivitis, Allergic ; Eosinophil Cationic Protein* ; Humans ; Keratoconjunctivitis*

PURPOSE: We report a case of toxic keratoconjunctivitis resulting from the self-application of human breast milk as a traditional folk remedy for allergic conjunctivitis. CASE SUMMARY: An 82-year-old woman presented with pain and conjunctival hyperemia in the right eye that had been worsening for three days. Two months previously, she was treated with antiallergic eye drops for allergic conjunctivitis at another eye clinic. However, the symptoms did not improve. She applied her daughter-in-law's breast milk into her right eye as a folk remedy for three days. The pain and conjunctival hyperemia worsened. At the initial visit, her corrected visual acuity was 0.3 in the right eye. Slit lamp examination demonstrated conjunctival hyperemia, punctate epithelial erosion at the central cornea, corneal keratic precipitates and white-colored deposits in the peripheral cornea combined with irregularly shaped small nodules. There was no anterior chamber inflammation. There was no medical history of rheumatoid arthritis or tuberculosis. Blood tests for serum and other infectious and inflammatory levels for infection and inflammatory markers were performed followed by application of topical steroids and antibiotics with artificial tears. After 3 weeks of treatment, conjunctival hyperemia and corneal deposits had almost resolved and best-corrected visual acuity improved to 1.0. CONCLUSIONS: The self-application of human breast milk may cause toxic keratoconjunctivitis. Therefore, efforts should be made, actively, to inform and educate the elderly in rural areas not to use human breast milk as a folk remedy.
Aged ; Aged, 80 and over ; Anterior Chamber ; Anti-Bacterial Agents ; Arthritis, Rheumatoid ; Breast ; Conjunctivitis, Allergic ; Cornea ; Female ; Hematologic Tests ; Humans ; Hyperemia ; Inflammation ; Keratoconjunctivitis ; Lubricant Eye Drops ; Medicine, Traditional ; Milk, Human ; Ophthalmic Solutions ; Slit Lamp ; Steroids ; Tuberculosis ; Visual Acuity

Thygeson(1950) reported the specific type of superficial punctate keratitis and suspected this probably come from viral origin. Braley(1953) described and illustrated well about the superficial punctate keratitis in his article. According to his paper, its diagnostic features can be summarized as follows; 1) Appearance as a chronic, bilateral punctate epithelial keratitis. 2) Long duration. 3) Eventual healing without scar. 4) Lack of response to systemic or topical antibiotics or sulfonamide, or to removal of corneal epithelium. 5) Striking symptomatic response to topical steroids. These diagnostic features form the basis for this report. 249 cases of this disease has been observed during the period of this study(October, 1960-July, 1962). 25 cases were available for detailed clinical and laboratory analysis. Following observations were made. 1) Corneal punctate opacity is strictly epithelial and located mostly in lower quadrant in chronic cases without associated conjunctivitis. 2) None of the cases gave a history of acute onset. 3) Long duration with remission and exacerbation. 4) No response to topical antibiotics or sulfonamide, or to removal of corneal epithelium. Remarkable symptomatic response to topical steriod. 5) No seasonal and professional relationship with this disease. 6) Normal conjunctival bacterial flora in simple culture. None of them showed inclusion bodies or significant cytological changes. No lesions developed in rabbit cornea inoculartion using scraping from conjunctiva and cornea. 7) Healing without scar. 8) Further detailed virological studies are needed for determinning the etiological agent.
Anti-Bacterial Agents ; Cicatrix ; Conjunctiva ; Conjunctivitis ; Cornea ; Epithelium, Corneal ; Inclusion Bodies ; Keratitis* ; Seasons ; Steroids ; Strikes, Employee

Conjunctival Diseases/etiology* ; Humans ; Laryngeal Diseases/etiology*