OBJECTIVETo study the changes in bulbar conjunctiva microcirculation (BCM) and the therapeutic effect of Pentoxifylline on BCM disturbance after high-voltage electrical burn (HEB) in rabbits.

METHODSForty-five rabbits were divided into control group (C), electrical burn group (EB), and Pentoxifylline treatment group (PT) according to random number table, with 15 rabbits in each group. Model of HEB was reproduced in rabbits from EB and PT groups with voltage regulator and experimental transformer. Rabbits in C group were sham injured with the same devices without electrification. Changes in BCM were observed with microcirculation microscope at 15 minutes before HEB and 5 minutes, 1, 2, 4, 8 hour(s) post HEB (PHM or PHH), including: (1) morphology of microvessels, such as the discernible, diameters of arterioles, venules, and capillaries, the unevenness in caliber, and ischemic area; (2) dynamic changes in microvascular blood flow, such as blood flow speed in arterioles, venules, and capillaries, erythrocyte aggregation, and microthrombi formation; (3) condition of tissues surrounding microvessel, such as bleeding and exudation. Measurement data were processed with t test; enumeration data were processed with Fisher's exact test.

RESULTS(1) Morphology of microvessel: discernible of microvessels in EB and PT groups was decreased, but that of PT group was better than that of EB group. At PHM 5, diameter of arterioles, venules and capillaries was respectively (7.3+/-2.5), (12.3+/-2.4), (3.5+/-0.7) microm in EB group, all narrower than those of the control group [(14.6+/-3.1), (27.2+/-3.5), (9.0+/-1.4) microm, with t value respectively 5.23, 13.66, 14.04, P values all below 0.05]. Diameters of the microvessels in PT group [(10.2+/-3.8), (21.5+/-3.1), (7.1+/-1.2) microm] were larger than those in EB group (with t value respectively 2.21, 8.99, 10.18, P values all below 0.05). Diameters of arterioles, venules and capillaries in EB and PT groups recovered to the before HEB size at PHH 1. From PHH 2 to 8, arterioles and capillaries decreased gradually in caliber, venules dilated gradually in EB and PT groups, but the changes in PT group were not obvious. Thickness of microvessel was observed uneven in EB group at PHM 5, which lasted until PHH 8. Ischemia of the tissue was observed in EB group at PHM 5, which improved at PHH 2. Situation in PT group was better. (2) Dynamic changes in microvascular blood flow: at PHM 5, blood flow speed in arterioles, venules and capillaries was respectively (202+/-53), (198+/-44), (46+/-12) microm/s in EB group, all slower than those of the control group [(544+/-37), (359+/-32), (220+/-19) microm/s, with t value respectively 20.47, 11.51, 30.02, P values all below 0.05], and those of PT group [(335+/-42), (260+/-35), (119+/-23) microm/s] were faster than those of EB group (with t value respectively 7.55, 4.26, 14.85, P values all below 0.05). Blood flow speed in EB and PT groups recovered to the before HEB level at PHH 1. From PHH 2 to 8, blood flow speed decreased gradually in EB and PT groups, but that of PT group was faster than that of EB group. Erythrocyte aggregation in venules and capillaries was observed in EB group at PHM 5, which eased up at PHH 1, but aggregated at PHH 2, lasting until PHH 8. Obvious microthrombi were observed in EB group at PHH 2, which increased gradually. These changes were less obvious in PT group. (3) Condition of surrounding tissues of microvessel: in EB group, exudation was observed around microvessels at PHH 1, bleeding at PHH 2, with a worsening tendency. Changes in those in PT group were less obvious.

CONCLUSIONSHEB causes disturbance in BCM, but it can be ameliorated by Pentoxifylline.

Animals ; Burns, Electric ; drug therapy ; pathology ; Conjunctiva ; blood supply ; Microcirculation ; Microvessels ; pathology ; Pentoxifylline ; therapeutic use ; Rabbits

Conjunctival tuberculosis is a very rare disease, but importance of tuberculosis in Korea is high. We experienced a 49 year-old female patient with a small mass like lesion at inferior fornix with conjunctivitis. We performed excisional biopsy. Biopsy result was conjunctival tuberculosis. So we searched other body tuberculosis focus, but, not found. we emphasize differential diagnosis chronic conjunctivitis and conjunctival tuberculosis. Brief clinical history and clinical findings will be presented with pictures of the pathology findings and some literatures will be reviewd.
Biopsy ; Conjunctiva ; Conjunctivitis ; Diagnosis, Differential ; Female ; Humans ; Korea ; Middle Aged ; Pathology ; Rare Diseases ; Tuberculosis*

The study of chronic dacryocystitis: Identification of pathogenic organism and histopathological findings. Primary acquired nasolacrimal duct obstruction is a clinical syndrome of unknown cause, and the histopathology of the nasolacrimal duct has not been substantially studied. The intent of this study was to determine what organisms grow in the lacrimal sac of patients ewquirirng dacryocystorhinostomy. It was also investigated whether or not there was a correlation between organisms cultured from the sac and from the conjunctiva. The tissue fo lacrimal sac was examined histopathologically. A total of 32 patients who had received dacryyocystorhinostomy from January 1998 to July 1998 were included. The result of the study indicate thath there is not a clinically significant correlation of pathogenic organism between conjunctiva and lacrimal sac. Most patients had histopathologic findings consisted of inflammation or fibrosis of the lacrimal sac. The incidence of significant pathology of the lacrimal sac is low. However, one case was identified as an adenocarcinoma by routine biopsy of the lacrimal sac during dacryocystorhinostomy.
Adenocarcinoma ; Biopsy ; Conjunctiva ; Dacryocystitis* ; Dacryocystorhinostomy ; Fibrosis ; Humans ; Incidence ; Inflammation ; Nasolacrimal Duct ; Pathology

PURPOSE: To assess the occurrence and characteristics of benign eyelid and conjunctival tumors. METHODS: We histopathologically analyzed 80 consecutive cases of benign eyelid (56 cases) and conjunctival (24 cases) tumors between April 2000 and November 2002. All eyelid and conjunctival tumors were confirmed histopathologically. Age, sexual distribution, occurrence and location were analyzed. RESULTS: The mean ages were 42.32+/-16.27 SD in benign eyelid tumors, and 29.71+/-18.60 SD in benign conjunctival tumors. The sexual distribution revealed 23 males and 33 females in benign eyelid tumors, and 12 males and 12 females in benign conjunctival tumors. About the location, lower lid was most frequent site of benign eyelid tumors, responsible for 27 (48.2%) of the 56 cases, and medial conjunctiva was the most frequent site of benign conjunctival tumors, responsible for 14 (58.3%) of 24 cases. Shave biopsy was performed in eyelid margin tumors. Excisional biopsy was performed in other eyelid tumors and conjunctival tumors. The frequency of benign eyelid tumors was noted in this order: intradermal nevi 25 cases (44.6%), seborrheic keratoses 9 cases (16.1%), and compound nevi 6 cases (10.7%), and frequency of benign conjunctival tumors was noted in this order: compound nevi 7 cases (29.2%), and intradermal nevi 6 cases (25.0%). CONCLUSIONS: From the Results of this study, we hope that this report provides a basic source for diagnosis and therapy of the benign eyelid and conjunctival tumors.
Biopsy ; Conjunctiva ; Diagnosis ; Eyelids* ; Female ; Hope ; Humans ; Keratosis, Seborrheic ; Male ; Nevus ; Nevus, Intradermal ; Pathology

In the process of closing scleral wounds caused by various conditions, incarceration of conjunctiva, Tenon's capsule, or vitreous in the wound can occur unexpectedly. We created such conditions experimentally in order to discover their intraocular complications. The experimental materials consisted of 12 albino rabbits (24 eyes) divided into two groups (Groups I & II). Vitrectomy was performed in the Group I rabbits (12 eyes) but not in the Group II rabbits (12 eyes). Flaps of conjunctiva and Tenon's capsule were made and inserted into the vitreous cavity through the sclerotomy site, which was soon closed. Fundal examination of the rabbits was carried out using an indirect ophthalmoscope at intervals after the procedure; first at 3 days, then at 1, 3, and 6 weeks, and then at 3 months and 6 months, respectively. Enucleation of the rabbits' eyes 4 from two different rabbits at each of these intervals was carried out, and the extracted eyes were examined under a light microscope at each interval. The results are summarized as follows: 1. All rabbit eyes studied showed intraocular fibrovascular proliferation. 2. The extent of tissue proliferation, which was proportional to the amount of vitreous hemorrhage, was greater in Group II than in Group I. 3. The proliferated tissue developed to "band" by three weeks postexperiment, after which it gradually regressed. 4. The fibrovascular band was made of fibroblasts, stromal matrix, and capillaries.
Animals ; Cell Division ; Conjunctiva/pathology ; Eye Injuries/surgery ; Fundus Oculi ; Postoperative Complications ; Rabbits ; Retinal Diseases/pathology ; Retinal Vessels/*pathology ; Sclera/*surgery ; Vitrectomy ; Vitreous Hemorrhage/pathology

PURPOSE: Recently, conjunctivochalasis repair surgery using electrocauterization has been gaining popularity. However, patients with electrocauterized conjunctivoplasty tend to complain of more postoperative pain than patients undergoing simple excision with suturing. Therefore, we investigated the effects of electrocauterization on inflammation of the conjunctiva using an experimental animal model and compared these with the effects of simple excision with suturing. METHODS: Ten New Zealand white rabbits underwent cauterization in the right eyes and excision and suturing in the left eyes. For each eye, we excised or electrocauterized the inferior bulbar conjunctiva, 1 mm in width and 6 mm in length, 2 mm from the limbus. A fine-needle electrode was inserted subconjunctivally, and electrocauterization was performed. In the contralateral eye, the corresponding area was excised and re-approximated with 10-0 nylon sutures. Sutures were removed after 14 days. Tissue samples were obtained at 21 days post-procedure, and inflammatory cells were counted in five randomly selected fields (x200) on hematoxylin-eosin stained slides. Tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta concentrations in tears were measured using enzyme linked immunosorbent assays. RESULTS: All cauterized eyes demonstrated smooth surface healing without scarring after 5 days, whereas sutured eyes presented with mild edema with some scarring until the suture was removed. The number of inflammatory cells was significantly greater in sutured eyes compared with cauterized eyes (p = 0.035, Mann-Whitney U-test) at 21 days post-procedure. Tear TNF-alpha and IL-1beta concentrations at 21 days were similar in both groups. CONCLUSIONS: Electrocauterization for conjunctivoplasty seems to be advantageous in terms of inflammation compared with simple suturing and excision.
Animals ; Conjunctiva/pathology ; Conjunctivitis/*pathology/*surgery ; Disease Models, Animal ; Edema/pathology/prevention & control ; Electrocoagulation/*methods ; Humans ; Male ; Pain, Postoperative/pathology/*prevention & control ; Rabbits ; *Suture Techniques ; Treatment Outcome

Sodium hyaluronate can be used during a trabeculectomy to prevent early postoperative hypotony and shallow anterior chamber. To determine its long-term effect on the outcome of filtration surgery, fifteen rabbits underwent a osterior-lip sclerectomy in both eyes. Into their right eyes 0.2ml sodium hyaluronate, and into left eyes 0.2ml balanced salt solution were injected intracamerally. Gross and histopathological differences of bleb were observed. There was no statistically significant difference in longevity of the filtration bleb.
Animals ; Anterior Chamber/*drug effects/pathology ; Conjunctiva/pathology ; Connective Tissue/pathology ; Follow-Up Studies ; Hyaluronic Acid/*administration & dosage ; Ocular Hypotension/prevention & control ; Rabbits ; Sclera/pathology ; *Sclerostomy

A 35-year-old woman presented with history of a painless, slow-growing nodule in a left eye caruncle over the last 2 months. The visual acuity was 20 / 20 and the ocular movements were in the normal range. The venereal disease research laboratory test, erythrocyte sedimentation rate, serum angiotensin converting enzyme level, and chest radiograph were all normal. An excisional biopsy was done to confirm the diagnosis. A 1.3 x 0.5 x 0.3 cm sized nodule was extracted and sent for histopathologic examination. Hematoxylin-eosin staining showed follicular hyperplasia with perifollicular fibrosis, an eosinophil infiltrate, and proliferation of capillary vessels. The capillaries were lined by normal-appearing, flat, spindle-shaped endothelial cells. On the basis of these histopathologic findings, the diagnosis of Kimura disease in a caruncle was established. This is the first report describing Kimura disease localized to a caruncle. Kimura disease should be suspected and included in the differential diagnosis of a caruncular mass lesion.
Adult ; Angiolymphoid Hyperplasia with Eosinophilia/*diagnosis ; Asian Continental Ancestry Group ; Conjunctiva/*pathology ; Conjunctival Diseases/*diagnosis ; Diagnosis, Differential ; Female ; Humans

A 35-year-old woman presented with history of a painless, slow-growing nodule in a left eye caruncle over the last 2 months. The visual acuity was 20 / 20 and the ocular movements were in the normal range. The venereal disease research laboratory test, erythrocyte sedimentation rate, serum angiotensin converting enzyme level, and chest radiograph were all normal. An excisional biopsy was done to confirm the diagnosis. A 1.3 x 0.5 x 0.3 cm sized nodule was extracted and sent for histopathologic examination. Hematoxylin-eosin staining showed follicular hyperplasia with perifollicular fibrosis, an eosinophil infiltrate, and proliferation of capillary vessels. The capillaries were lined by normal-appearing, flat, spindle-shaped endothelial cells. On the basis of these histopathologic findings, the diagnosis of Kimura disease in a caruncle was established. This is the first report describing Kimura disease localized to a caruncle. Kimura disease should be suspected and included in the differential diagnosis of a caruncular mass lesion.
Adult ; Angiolymphoid Hyperplasia with Eosinophilia/*diagnosis ; Asian Continental Ancestry Group ; Conjunctiva/*pathology ; Conjunctival Diseases/*diagnosis ; Diagnosis, Differential ; Female ; Humans

BACKGROUNDRupture of the medial canthal ligament can be caused by many events. It remains a challenge to rebuild the drainage system and restore the function. The aim of this study was to evaluate the clinical efficacy of medial canthoplasty combined with conjunctivodacryocystorhinostomy (CDCR) in patients with medial telecanthal deformities and lacrimal drainage system damage.

METHODSTwenty-two patients (22 eyes) treated with medial canthoplasty and CDCR during June 2012 to June 2014 were included in this retrospective study. For all patients, a self-tapping, titanium, low-profile head microscrew was drilled into the solid bone on the posterior aspect of the anterior lacrimal crest at the attachment position of the medial canthal ligament. Medpor-coated tear drainage tubes were applied. Distance of patient's lateral displacement before and after operation was recorded and compared. The complications of CDCR were described.

RESULTSBefore the surgery, distance of patient's canthal displacement was 4-6 mm. The canthal distance between two eyes of patients with surgery was 1 mm or less. Among patients with CDCR, four patients had proximal obstruction and two patients had distal obstruction. Five patients had tube malposition, for example, tube extrusion 1-3 months after surgery.

CONCLUSIONSMedial canthoplasty combined with CDCR is an effective surgical method for treatment of patients with medial telecanthal deformity and lacrimal drainage system obstruction. The study indicates that medial canthoplasty combined with CDCR surgery rebuilds normal appearance of eyelid and contour of the medial canthus and successfully repairs the function of the lacrimal drainage system.

Adult ; Aged ; Blepharoplasty ; methods ; Conjunctiva ; pathology ; surgery ; Dacryocystorhinostomy ; methods ; Female ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Treatment Outcome