Penetrating keratoplasty was performed in six eyes of granular corneal dystrophy. Vision was improved from worse than 0.05 to better than 0.4. Mean follow-up period is 14 months (12-18 months). Corneal deposits were stained for H and E. Masson trichrome, Wilder's reticulin and Luxol fast blue but not for PAS, Congo red and Oil red a stain. Electron micorscopic examination showed that polymorphic, electron dense rod-shaped bodies were present in the Bowman's layer and between stromal lamellae and in the interceliular space of corneal epithelium. And, for the first time we found a area looked like a transformation from normal stroma to the granular deposit.
Congo Red ; Epithelium, Corneal ; Follow-Up Studies ; Keratoplasty, Penetrating ; Reticulin

Amyloidosis is characterized by the deposition of extracellular fibrillar proteins in various tissues. Amyloidosis in the head and neck is a rare and benign condition that usually takes the form of localized amyloidosis. Among primary sites of head and neck area, nasopharyngeal amyloidosis has proven to be rare and there are only about ten cases that have been reported in the literature worldwide until today. We report a case of localized nasopharyngeal amyloidosis. Histopathologic examination of the mass revealed findings consistent with amyloidosis, which were stained with Congo Red. The postoperative recovery was uneventful. At the present time, the patient has no evidence of disease or recurrence and complication.
Amyloidosis* ; Congo Red ; Head ; Humans ; Nasopharynx ; Neck ; Recurrence

To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from 15~35degrees C. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at 25degrees C for 5 days, the clear zone indicative of cellulolytic activity was more pronounced.
Benzenesulfonates ; Carbon ; Cellulose ; Congo Red ; Diminazene ; Hydrogen-Ion Concentration ; Phenolsulfonphthalein

To evaluate which dye is effective in a plate assay for detecting extracellular cellulase activity produced by fungi, four chromogenic dyes including remazol brilliant blue, phenol red, congo red, and tryphan blue, were compared using chromagenic media. For the comparison, 19 fungal species belonging to three phyla, ascomycota, basidiomycota, and zygomycota were inoculated onto yeast nitrogen-based media containing different carbon substrates such as cellulose (carboxylmethyl and avicel types) and cellobiose labeled with each of the four dyes. Overall, the formation of clear zone on agar media resulting from the degradation of the substrates by the enzymes secreted from the test fungi was most apparent with media containing congo red. The detection frequency of cellulase activity was also most high on congo red-supplemented media. The results of this study showed that congo red is better dye than other three dyes in a plate assay for fungal enzyme detection.
Agar ; Ascomycota ; Basidiomycota ; beta-Glucosidase ; Carbon ; Cellobiose ; Cellulase ; Cellulases* ; Cellulose ; Coloring Agents* ; Congo ; Congo Red ; Fungi* ; Phenolsulfonphthalein ; Yeasts

Primary nodular cutaneous amyloidosis is a very rare type of amyloidosis, it must excluded the secondary nodular deposition of systemic amyloidosis. We report herein 47-year-old male with a erythematous waxy nodule on his parietal scalp for one year. Histopathologic examination showed homogenous amorphous eosinophilic deposition in the dermis with nodular fashion. In Congo red, amyloid material stained positively and showed apple-green birefringence with polarized light. On electron microscopic examination, extensive deposit of amyloid with typical 6-10 nm filament was confirmed. He had neither clinical nor laboratory evidence of systemic amyloidosis. We reviewed previously reported nodular amyloidosis in Korea.
Amyloid ; Amyloidosis* ; Birefringence ; Congo Red ; Dermis ; Eosinophils ; Humans ; Korea ; Male ; Middle Aged ; Rabeprazole ; Scalp

Amyloid may involve the conjunctiva without deposits of the skin of the eyelid in primary localized amyloidosis, but extremely rare in primary systemic amyloidosis. We describe the clinicopathologic features of a case of primary systemic amyloidosis which invloved the conjunctiva and larynx. A 58-years-old woman presented a conjuctival mass of the left eye. Slit lamp microscopy showed a pinkish, flat elevated mass on the bulbar conjunctiva, fornix, and upper tarsal conjunctiva. There was no history of antecedent ocular or systemic diseases. She had undergone total laryngectomy for laryngeal amyloidosis. Microscopically, a pale amorphous eosinophilic material was deposited in the subcojunctival tissue. The deposit showed metachromasia with crystal violet, and dichroism and birefringence in polarized light following Congo red staining. Electron microscopically amyloid was comprised of linear add non-branching fibrils, which were about 11nm in width.
Amyloid ; Amyloidosis* ; Birefringence ; Congo Red ; Conjunctiva* ; Eosinophils ; Eyelids ; Female ; Gentian Violet ; Humans ; Laryngectomy ; Larynx ; Microscopy ; Skin

A 38-year-old male patient visited our clinic complaining of three skin lesions on the scalp. There were yellowish to brownish, waxy, non-tender, walnut-sized nodules. Hematoxylin and eosin staining revealed amorphous pinkish material deposits in the dermis. The Congo red stain and Dylon stain under polarizing microscopy showed yellow-green birefringence and the immunoglobulin-lambda light chain stain showed a positive reaction. An electron microscopic examination revealed filaments with uniform diameter(6 to 10nm) that were straight and neither branched nor anastomosed. Based on the clinical, histopathological, immunohistochemical and electron microscopical findings, the skin lesions were diagnosed as nodular amyloidosis.
Adult ; Amyloidosis* ; Birefringence ; Congo Red ; Dermis ; Eosine Yellowish-(YS) ; Hematoxylin ; Humans ; Male ; Microscopy ; Scalp ; Skin

We report a case of anosacral cutaneous amyloidosis in a 74 year-old-man. The skin finding is a patch of the size of a childs palm on the sacral area, consisting of brownish macular lines that radiate frorn the anus. Histopathology revealed defiosits of homogenous eosinophilic mateial s in the papillary dermis. Congo red staining and dylon staining was positive. Immuohistochemical staining using anti SAP antibodies and anti-keratin antibodies showed positive reactions.
Amyloidosis* ; Anal Canal ; Antibodies ; Child ; Congo Red ; Dermis ; Eosinophils ; Humans ; Skin

Imaging distribution of beta-amyloid plaques in Alzheimer's disease is very important for early and accurate diagnosis. Early trial of the beta-amyloid plaques includes using radiolabeled peptides which can be only applied for peripheral beta-amyloid plaques due to limited penetration through the blood brain barrier (BBB). Congo red or Chrysamine G derivatives were labeled with Tc-99m for imaging beta-amyloid plaques of Alzheimer patient's brain without success due to problem with BBB penetration. Thioflavin T derivatives gave breakthrough for beta-amyloid imaging in vivo, and a benzothiazole derivative [C-11]6-OH-BTA-1 brought a great success. Many other benzothiazole, benzoxazole, benzofuran, imidazopyridine, and styrylbenzene derivatives have been labeled with F-18 and I-123 to improve the imaging quality. However, [C-11]6-OH-BTA-1 still remains as the best. However, short half-life of C-11 is a limitation of wide distribution of this agent. So, it is still required to develop an Tc-99m, F-18 or I-123 labeled agent for beta-amyloid imaging agent.
Alzheimer Disease ; Blood-Brain Barrier ; Brain ; Congo Red ; Diagnosis ; Half-Life ; Peptides

We described a case of diffuse biphasic cutaneous amyloidosis, a unique form of localized cutaneous amyloidosis. A 41-year-old man has gradually developed a lichenoid papular and a grouped spotted pigmented macular eruption on the trunk and upper extremities over the past 15 years. Histopathologic examination revealed that amyloid deposits were present in the papillary dermis. It was confirmed by Congo red staining, immunohistochemistry and electron microscopy. There was no evidence of systemic amyloidosis.
Adult ; Amyloidosis* ; Congo Red ; Dermis ; Humans ; Immunohistochemistry ; Microscopy, Electron ; Plaque, Amyloid ; Upper Extremity