Animals ; Hemorrhagic Fever Virus, Crimean-Congo ; Humans

Penetrating keratoplasty was performed in six eyes of granular corneal dystrophy. Vision was improved from worse than 0.05 to better than 0.4. Mean follow-up period is 14 months (12-18 months). Corneal deposits were stained for H and E. Masson trichrome, Wilder's reticulin and Luxol fast blue but not for PAS, Congo red and Oil red a stain. Electron micorscopic examination showed that polymorphic, electron dense rod-shaped bodies were present in the Bowman's layer and between stromal lamellae and in the interceliular space of corneal epithelium. And, for the first time we found a area looked like a transformation from normal stroma to the granular deposit.
Congo Red ; Epithelium, Corneal ; Follow-Up Studies ; Keratoplasty, Penetrating ; Reticulin

Amyloidosis is characterized by the deposition of extracellular fibrillar proteins in various tissues. Amyloidosis in the head and neck is a rare and benign condition that usually takes the form of localized amyloidosis. Among primary sites of head and neck area, nasopharyngeal amyloidosis has proven to be rare and there are only about ten cases that have been reported in the literature worldwide until today. We report a case of localized nasopharyngeal amyloidosis. Histopathologic examination of the mass revealed findings consistent with amyloidosis, which were stained with Congo Red. The postoperative recovery was uneventful. At the present time, the patient has no evidence of disease or recurrence and complication.
Amyloidosis* ; Congo Red ; Head ; Humans ; Nasopharynx ; Neck ; Recurrence

To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from 15~35degrees C. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at 25degrees C for 5 days, the clear zone indicative of cellulolytic activity was more pronounced.
Benzenesulfonates ; Carbon ; Cellulose ; Congo Red ; Diminazene ; Hydrogen-Ion Concentration ; Phenolsulfonphthalein

To evaluate which dye is effective in a plate assay for detecting extracellular cellulase activity produced by fungi, four chromogenic dyes including remazol brilliant blue, phenol red, congo red, and tryphan blue, were compared using chromagenic media. For the comparison, 19 fungal species belonging to three phyla, ascomycota, basidiomycota, and zygomycota were inoculated onto yeast nitrogen-based media containing different carbon substrates such as cellulose (carboxylmethyl and avicel types) and cellobiose labeled with each of the four dyes. Overall, the formation of clear zone on agar media resulting from the degradation of the substrates by the enzymes secreted from the test fungi was most apparent with media containing congo red. The detection frequency of cellulase activity was also most high on congo red-supplemented media. The results of this study showed that congo red is better dye than other three dyes in a plate assay for fungal enzyme detection.
Agar ; Ascomycota ; Basidiomycota ; beta-Glucosidase ; Carbon ; Cellobiose ; Cellulase ; Cellulases* ; Cellulose ; Coloring Agents* ; Congo ; Congo Red ; Fungi* ; Phenolsulfonphthalein ; Yeasts

OBJECTIVES: This study aims to evaluate the economic efficiency of a nutrition management program for children under 5 years of age in Kenge, Kwango District, Democratic Republic of the Congo (DR Congo) from 2014 to 2016. METHODS: The economic efficiency of a nutrition management program for children under 5 years of age who have recovered from malnutrition status was evaluated using a cost-benefit analysis. The costs were analyzed according to the executed budget incurred during the project period. The benefits were estimated as the monetary value of the saved lives of children under 5 years of age. The economic efficiency of the program was determined by the Benefit-Cost Ratio (BCR). The BCR was calculated by dividing the total discounted benefit by the total discounted costs. The project is economically efficient when the BCR is greater than 1. RESULTS: The costs of the nutrition management program were calculated as 1,677,609,648 Korean Won (KRW). A total of 2,466 children survived with improved malnutrition status through this program. The benefit for the reduction of mortality for children under 5 years of age was estimated to be 6,814,354,467 KRW, the estimated value of life for 2,466 children. The BCR was 4.06. CONCLUSIONS: The nutrition management program for children under 5 years of age in DR Congo was found to be a cost-effective project. Successful and efficient Official Development Assistance (ODA) for a health project requires integrated and comprehensive strategies and specialized international development consulting to improve efficiency. Future nutrition management programs should take into account the national health program to maintain the sustainability of the project.
Budgets ; Child ; Congo ; Cost-Benefit Analysis ; Democratic Republic of the Congo ; Humans ; Malnutrition ; Mortality ; National Health Programs ; Value of Life

OBJECTIVES: This study aims to evaluate the economic efficiency of a nutrition management program for children under 5 years of age in Kenge, Kwango District, Democratic Republic of the Congo (DR Congo) from 2014 to 2016. METHODS: The economic efficiency of a nutrition management program for children under 5 years of age who have recovered from malnutrition status was evaluated using a cost-benefit analysis. The costs were analyzed according to the executed budget incurred during the project period. The benefits were estimated as the monetary value of the saved lives of children under 5 years of age. The economic efficiency of the program was determined by the Benefit-Cost Ratio (BCR). The BCR was calculated by dividing the total discounted benefit by the total discounted costs. The project is economically efficient when the BCR is greater than 1. RESULTS: The costs of the nutrition management program were calculated as 1,677,609,648 Korean Won (KRW). A total of 2,466 children survived with improved malnutrition status through this program. The benefit for the reduction of mortality for children under 5 years of age was estimated to be 6,814,354,467 KRW, the estimated value of life for 2,466 children. The BCR was 4.06. CONCLUSIONS: The nutrition management program for children under 5 years of age in DR Congo was found to be a cost-effective project. Successful and efficient Official Development Assistance (ODA) for a health project requires integrated and comprehensive strategies and specialized international development consulting to improve efficiency. Future nutrition management programs should take into account the national health program to maintain the sustainability of the project.
Budgets ; Child ; Congo ; Cost-Benefit Analysis ; Democratic Republic of the Congo ; Humans ; Malnutrition ; Mortality ; National Health Programs ; Value of Life

We described a case of diffuse biphasic cutaneous amyloidosis, a unique form of localized cutaneous amyloidosis. A 41-year-old man has gradually developed a lichenoid papular and a grouped spotted pigmented macular eruption on the trunk and upper extremities over the past 15 years. Histopathologic examination revealed that amyloid deposits were present in the papillary dermis. It was confirmed by Congo red staining, immunohistochemistry and electron microscopy. There was no evidence of systemic amyloidosis.
Adult ; Amyloidosis* ; Congo Red ; Dermis ; Humans ; Immunohistochemistry ; Microscopy, Electron ; Plaque, Amyloid ; Upper Extremity

PURPOSE: To report a case of vitreous amyloidosis. METHODS: A 51-year old male presented with bilateral floater and decreased visual acuity of 3 months duration. Small white dots attached to the posterior lens surface were observed on slit lamp examination. The vitreous opacity, which connected with the small white dots to the posterior lens surface, were observed on funduscopic examination, and multiple hyperechoic plaques in the vitreous cavity on B-scan. The pars plana vitrectomy was performed. RESULTS: The aspirate of the pars plana vitrectomy was stained with Congo red and observed with light microscope, and polarized light. Electron microscopy was also performed. The vitreous smear stained with Congo red showed greenish dichroism and birefringent nature with polarized light and fine fibrillar nature of amyloid material was noted on electron microscopy. CONCLUSIONS: To our knowledge, this is the first Korean report of vitreous amyloidosis. The authors diagnosed vitreous amyloidosis that developed in a 51-year-old male patient. The diagnosis was confirmed by light microscope, polarized light and electron microscope.
Amyloid ; Amyloidosis* ; Birefringence ; Congo Red ; Diagnosis ; Humans ; Male ; Microscopy, Electron ; Middle Aged ; Visual Acuity ; Vitrectomy

Amyloidosis is a disease complex associated with deposition of insoluble filbrillar protein in various tissues of the body. Cutaneous lesions are present in up to 40 % of patients with primary and myeloma associated systemic amyloidosis. We present a case of systemic amyloidosis associated with multiple myleoma occuring in 54-year-old male who had waxy papules with petechiae on nasola bial fold, postauricular area, and cheek. The diagnosis was confirmed by Congo red stain, electron microscope, immunelectrophoresis, Bence-Jones proteinuria, immunoperoxidase stain, and bone marow biopsy.
Amyloidosis* ; Biopsy ; Cheek ; Congo Red ; Diagnosis ; Humans ; Male ; Middle Aged ; Multiple Myeloma* ; Proteinuria ; Purpura