Objective:To study the effects of deoxyadenosine(dAdo) on methotrexate (MTX) induced suppression of inflammatory bone destruction.Methods:The culture system of whole bone marrow cells (WBMCs) was utilized to evaluate osteoclastogenesis.TRAP staining and μCT analysis were utilized to evaluate osteoclastogenesis and bone destruction in adjuvant arthritis rats.Results:In the bone marrow culture system,MTX-induced suppression of osteoclastogenesis was abrogated by the addition of dAdo.dAdo canceled MTX-induced suppression of osteoclastogenesis in the rats with arthritis,and significantly abolished the therapeutic effects of MTX on inflammatory bone destruction in the rats.Conclusion:The accumulation of dAdo may be one cause of the losing effectiveness of MTX in bone destruction.

Objective:Analysis of anti-aging traditional Chinese medicine from the point of drug properties and explain the different types of drug characteristics.METHODS:The related literatures were derived from CNKI,and 51 traditional Chinese medicines were collected,then the ward method cluster analysis was carried out based on the property of a medicine data.Results:These drugs were divided into ten categories,the first kind of cold,bitter taste,liver meridian,can clear the liver;the second kinds of cold,bitter,spleen meridian,can clear splenopyretic;the third types of cold,sweet,nourishing yin;the fourth,five and six types were flat meridian,fiat sweet lung channel and flat sweet Spleen meridian,can nourishing liver,lung and spleen respectively;the seventh kinds of taste,to the kidney,can tonifying kidney-Qi;the eighth kinds of warm-natured,Spleen meridian,can fill the spleen meridian;the ninth kinds of warmnatured,spicy,to the kidney,tonifying kidney and dispelling cold;the tenth kinds of warm sweet to the kidney,tonifying kidney yang.Conclusion:The anti-aging traditional Chinese medicine was divided into ten categories based on the property of a medicine,the role of each type is different ways,reflecting the characteristics of anti-aging herbs.Adapt to the treatment of different aging patients.Through the classification of anti-aging drugs,it can be used to assist in the treatment of diseases,which provide clues for the study of anti-aging.

Objective To study the effect of diosgenin(Dio) from Dioscorea nipponica on the proliferation in mouse spleen T lymphocytes and expressions of IL-2 and IFN-γmRNA,in order to investigate the immunity regulatory mechanism of Dio.Methods T lymphocytes stimulated by different concentrations of Dio and concanavalin A(ConA) were co-cultured.CCK-8 was used to detect the Dio effects on T lymphocyte proliferation.The RT-PCR method was adopted to detect the effect of Dio on expression of IL-2 and IFN-γmRNA.Results The Dio concentration in the range of 0.937 5-15.000 0 μg/mL had the inhibiting effect on T lymphocyte proliferation,Dio concentration in the range of 3.750 0-15.000 0μg/mL had the inhibiting effect on IFN-γ and IL-2 expression in T lymphocytes.With the Dio concentration increase,the inhibition effect was enhanced(P＜0.05).7.500 0 μg/mL was the best inhibition concentration.The inhibition effect was decreased when the concentration exceeding 7.500 0 μg/mL.Conclusion Dio has the inhibiting effect on T lymphocyte proliferation and expressions of IL-2 and IFN-γ mRNA.

This study was aimed to explore the feasibility of identification of traditional Chinese medicine (TCM) constitution based on photoplethysmography (PPG) frequency domain analysis,and to establish an automatic identification system (AIS) of TCM constitution with simple operation and good generalization.The Classification and Determination of Constitution in TCM was used to evaluate the subjects' constitutional state.Pulse wave sensor was used to collect the pulse wave signal.Spectral characteristics were analyzed by power spectrum analysis.Eight classification algorithms of data mining were used to establish the model of qi-insufficiency constitution.The results showed that the pulse wave harmonic peak was that qi-insufficiency constitution was higher than health group;the model was optimal when random forest algorithm was used;and the accuracy of 10-CV test was 80.4％.Sensitivity reached 73.2％.Specificity and receiver operator characteristic were all above 0.8.The model has certain reliability.It was concluded that it was feasible to identify qiinsufficiency constitution by means of frequency domain analysis of PPG.It provides preliminary work for the construction of AIS of TCM constitution as well as a simple and quick assistant tool for the constitution identification of subhealth group in family health care.

OBJECTIVE: To explore the genetic etiology of a Chinese pedigree affected with pseudohypoparathyroidism. METHODS: Peripheral blood samples of the proband and his parents were collected and subjected to trio-whole exome sequencing (trio-WES). Candidate variants were verified among the pedigree and 50 randomly selected healthy individuals through analysis of restriction fragment length polymorphism. Short tandem repeat (STR) linkage analysis was used to verify the parental origin of the pathogenic variants. RESULTS: Trio-WES and Sanger sequencing showed that the proband and his mother had both harbored a c.121C>G (p.His41Asp) variant of the GNAS gene, which was not found in other family members and the 50 healthy controls. The variant was not found in international databases. Based on guidelines from the American College of Medical Genetics and Genomics, the variant was predicted to be likely pathogenic. CONCLUSION The novel c.121C>G variant of the GNAS gene probably underlay the disease in this pedigree. Above finding has enriched the spectrum of GNAS gene variants.
Female ; Humans ; Pedigree ; East Asian People ; Mothers ; Exome Sequencing ; Pseudohypoparathyroidism/genetics* ; Mutation ; China ; Chromogranins/genetics* ; GTP-Binding Protein alpha Subunits, Gs/genetics*

Objective:To analyze the pathogenic gene and prenatal diagnosis of a family with intellectual disability.Methods:Out of this family consisting of 17 members in three generations, four males had intellectual disability. The proband's elder sister (Ⅱ-7) visited Henan Provincial People's Hospital in Oct 2019 for genetic counseling at 8 weeks of gestation. After informed consent was obtained, peripheral blood samples of the family members were collected. The whole exome sequencing was performed on the genome DNA of the proband (Ⅱ-9, male) and his parents to screen the candidate variants for phenotype co-segregated analysis by Sanger sequencing. The expression vectors were constructed by homologous recombination and the splicing experiments were performed in vitro. Reverse transcription polymerase chain reaction, Sanger sequencing, and TA clone sequencing were used to analyze the effect of candidate variants on splicing. After the pathogenic variant was determined the proband's elder sister underwent prenatal diagnosis (Ⅲ-7) using goldeneyeTM20A genotyping system and Sanger sequencing. Results:A hemizygous synonymous variant of c.1302G>A (p. S434S) in DLG3 gene was found in the proband by whole exome sequencing, which was carried by his mother (Ⅰ-1) and co-segregated with the phenotype in other family patients. In vitro splicing experiment showed that c.1302G>A variant led to abnormal splicing of 88.24% transcripts, which further resulted in the reading frame shift and protein function impairment. The mutation was not detected in the fetus (Ⅲ-7), who was born alive later and showed no abnormal mental or behavioral development at the age of one and a half year and is still being followed up. Conclusions:The synonymous mutation c.1302G>A in DLG3 gene was the etiopathogenesis of X-linked intellectual disability in this family.