Objective To explore the effects of prenatal exposure to lipopolysaccharide (LPS) on insulin resistance in adult offspring rats. Methods Nulliparous, time-matched Sprague-Dawley rats were randomly divided into two groups (n=10 for each group):control group and LPS group. On the pregnant day 8, 10 and 12, rats in control group and LPS group were administered intraperitoneally with saline 0.5 mL or LPS 0.40 mg/kg, respectively. The blood levels of fasting plasma glucose (FPG), insulin and Leptin were detected at 90 days of age in offspring. The steady state insulin resistance index (HOMA-IR) and insulin sensitivity index (QUICKI) were evaluated in adult offspring rats. Results Compared with the control group, blood levels of FPG (mmol/L:7.72 ± 0.42 vs 7.02 ± 0.42), insulin (mIU/L:8.78 ± 4.10 vs 1.51 ± 0.27) and Leptin (μg/L:3.88 ± 1.40 vs 1.00±0.33) were significantly increased in offsping of LPS group, as well as HOMA-IR (3.01±1.41 vs 0.47±0.09) in-creased, while QUICKI (0.57±0.07 vs 0.99±0.08) decreased in offsping of LPS-treated rats. Conclusion Prenatal exposure to LPS can result in abnormality of insulin resistance in offspring rats.

AIM:In this study, the rat lung injury model was induced by ammonium chloride for studying the effect of imidapril on blood gas, serum TNF-α, IL-6 and MDA concentrations, and AngⅡand CD54 protein expression in rat lung tissue.METHODS:Male rats were randomly divided into 3 groups:control group, lung injury model group and drug group.The rats in control group were given saline (2 mL/kg), while the rats in lung injury model group were given 6% ammonium chloride (2 mL/kg).In drug group, imidapril (3 mg· kg-1· d-1) was given to the rats once daily for 1 week by intragastric gavage after given 6%ammonium chloride.On the 7th day, the rats were anesthetized with 2% so-dium pentobarbital.Abdominal aorta blood, venous blood and lung tissue were collected.The blood gas indexes and serum TNF-α, IL-6 and MDA concentrations were determined.The lung tissues were fixed and sliced, and the expression of AngⅡ and CD54 proteins was detected by immunohistochemistry.RESULTS: The PaCO2 increased in lung injury model group compared with control group and drug group (P<0.05).The expression of AngⅡand CD54, and the concentrations of TNF-α, IL-6 and MDA also increased significantly ( P<0.01) in model group.Pulmonary edema, inflammation, alveo-lus congestion, hemorrhage and hyperplasia in model group were obvious compared with control group and drug group. CONCLUSION:Imidapril improves blood gas indexes, and reduces lipid peroxidation and inflammatory responses in the rats with lung injury induced by ammonium chloride.

Objective To study the clinical effect of massage therapy on infants and young children with asthmatic disease.Methods 100 infants and young children with asthmatic disease were selected.The children and parents who agreed to cooperate with massage therapy were enrolled as treatment group.The children and parents who were unwilling to cooperate with massage therapy,but willing to cooperate with home aerosol therapy were enrolled as control group,50 cases in each group.The two groups of children took comprehensive treatment measures,the treatment group was given massage therapy,the control group was given home aerosol therapy.The therapeutic effects of the two groups were compared and analyzed.Results The respite time,recurrent frequency of wheezing within 1 year,respiratory infection occurred within 1 year,number of re-hospitalization within 1 year of the control group were (5.6 ± 1.36) d,(2.35 ± 1.13) times,(2.96 ± 1.22) times,(0.85 ± 0.58) times,which of the treatment group were (5.82 ± 1.44) d,(2.31 ± 1.09) times,(2.89 ± 1.19) times,(0.86 ± 0.51) times,the differences were not statistically significant between the two groups (t=0.573,0.417,0.523,0.872,all P ＞0.05).The peak time before treatment,1 month after treatment and 6 months after treatment,and the peak volume ratio before treatment,1 month after treatment and 6 months after treatment between the two groups had no statistically significant differences(all P ＞ 0.05).Conclusion Massage therapy can achieve the same effect with the home aerosol therapy,the method is simple,easy,without any adverse reactions,it is worthy of clinical promotion.