According to the sequence of SmLEA gene,a DNA fragment of 1 038 bp upstream of the coding sequence of SmLEA gene was amplified by DNA walking with the genomic DNA of Salvia miltiorrhiz as the template.Sequence analysis showed that the fragment contained some putative cis-elements relating to abiotic stress,ABA,seed specific expression.So the S.miltiorrhiz seedings was treated with 100?mol/L ABA,200mmol/L NaCl,4℃,and subjected to dehydration.The real-time PCR showed that expression levels of SmLEA was increased obviously,which was in accordance with the sequence analysis.

Objective To study the chronic neurotoxicity of aluminum through observing the adverse effect of long term intake of aluminum on the memory of rats.Methods 40 Wistar rats were randomly divided into 4 groups and given Al2(SO4)3 through drinking water for 6 months,the concentration of Al3+ in the waters were 0.12 mg/L,40 mg/L,400 mg/L and 2 000 mg/L respectively,then passive avoidance tests were carried out.One month before pregnancy,the dams were given Al3+ of 40 mg/L and 2 000 mg/L respectively,after the offspring were born,the group given high[Al3+] was subdivided into two groups,one continuously drank the water with high [Al3+],the other drank the water without Al3+.The dams drinking water with low [Al3+] and their offspring continued to drink the same water as before.At the age of three months,all the offspring were examined with passive avoidance tests.Results The memory acquirement in all adult rats were not abnormal,but memory retention were severely damaged (P0.05).The rats continuously drinking water with high [Al3+] after birth had significant damage both in memory acquirement and in retention (P

Child ; Humans ; Pneumonia ; diagnosis ; etiology ; Recurrence

AIM To investigate the exact subtype of ryanodine receptor (RyR) in pulmonary artery smooth muscle cell(PASMC) and the change of RyR expression from pulmonary hypertensive (PAH) rats. METHODS Rats pre treated with a single intraperitoneal injection of monocrotaline (MCT, 60 mg?kg -1 ) were used to produce PAH animal model and killed 21 d later. The subtype expression of RyR and the change of their mRNA were measured by RT PCR; protein expression of RyR was measured by Western blot in PASMC. RESULTS The PASMC of rats only expressed type ⅡRyR (RyR2). RT PCR showed the RyR2 mRNA concentration of PAH group was higher than that of control; Western blot demonstrated that the RyR2 protein level in PAH group was increased to (170 23?45 34)%. CONCLUSION PASMC only expressed RyR2 subtype and the increment of RyR2 expression may contribute to the pathological mechanism of PAH formation.

OBJECTIVE: To investigate the pharmacokinetics of citalopram hydrobromide(CTH)thermosensitive nasal gel and further evaluate its brain delivery in rats. METHODS: The concentrations of CTH in rat plasma and brain tissue were determined by HPLC method. With intragastric administration (ig) of CTH solution as control, CTH thermosensitive nasal gel was intranasally given to rats and the concentrations of CTH in plasma and brain tissues were then determined. Moreover, the main pharmacokinetic parameters of CTH in plasma and brain tissues such as tmax, ρmax,relative bioavailability (Fr) and drug targeting efficiency (DTE) were estimated. RESULTS: Main pharmacokinetic parameters of CTH following nasal and ig administration to rats such as tmax and ρmax were 5 and 45 min, 2 152.86 and 589.68 ng•mL-1 in plasma, and 5 and 45 min, 17 660.56 and 1 171.68 ng•g-1 in brain tissue, respectively. Finally, the Fr and DTE of CTH thermosensitive nasal gel were found to be 184.91% and 250.03%, respectively. CONCLUSION: CTH thermosensitive nasal gel may be an ideal non-oral new dosage form with many advantages such as rapid in vivo absorption, high bioavailability and obvious brain delivery characteristics.

OBJECTIVE: To optimize the formulation of citalopram hydrobromide (CTH ) thermosensitive nasal gel and further evaluate its in vitro properties. METHODS: With gelling temperature and gelling time as evaluating indexes, central composite design-response surface and single factor experimental design method were used to optimize the formulation of CTH thermosensitive nasal gel by using poloxamer 407(F127) and carbomer 940 (CP940) as gel materials. Meanwhile, nasal mucosa permeation enhancer for CTH was then sieved by using Franz diffusion cell and ex vivo sheep nasal mucosa as experimental model. Finally, CTH thermosensitive nasal gel was prepared with cold method and then its in vitro properties was evaluated. In vitro cumulative erosion and cumulative release rate of the drug thermosensitive nasal gel were investigated by membrane-free dissolution method and dialysis membrane method, respectively. Moreover, the effect of temperature and pH on the viscosity of the drug nasal gel formulation was also evaluated. RESULTS: The optimal formulation of the thermosensitive nasal gel consisted of CTH 8.0%, F127 20.27%, CP940 0.17%, DM-β-CD 3.0%, ethylparaben 0.05% and distilled water. The gelling temperature, gelling time and pH of the drug thermosensitive nasal gel were found to be about 32.5 ℃,42 s and 5.0, respectively. The in vitro cumulative erosion and cumulative release percentage were both greater than 90% in 55 min and furthermore there was good linear correlation between these two parameters (r=0.998 6). Additionally, in vitro cumulative release of the drug from the gel formulation was determined to be 92% within 8 h, which conformed to Higuchi kinetic equation. Furthermore, the viscosity of the drug nasal gel was influenced by temperature as well as pH in different extent. CONCLUSION: The optimized formulation of the CTH thermosensitive nasal gel with central composite design-response surface method and single factor design method shows suitable gelling temperature, gelling time, pH value for nasal preparation and obvious in vitro drug sustained release characteristics.

Adult ; Female ; Humans ; Ki-1 Antigen ; metabolism ; Lymph Nodes ; metabolism ; pathology ; surgery ; Lymphoma, Large-Cell, Anaplastic ; metabolism ; pathology ; surgery ; Neck ; Receptor Protein-Tyrosine Kinases ; metabolism

Fracture Fixation ; methods ; Humans ; Radius Fractures ; surgery

The practice and exploration of bilingual teaching for the course of microbiology has been made in order to improve the students foreign lingual level and to meet the higher requirement on tip-top person with the social development. As a result,bilingual teaching is welcome,and the teaching effect is so distinct that the aim was reached to either study the fundamental knowledge or enhance the English level.

Aim To investigate estradiol inhibition of neointimal proliferation after rat carotid artery balloon injury. Methods Eight to ten-week-old SD rats (male,n=21,female,n=21) were divided into intact control(n=7),gonadectomy control(n=7) and estradiol (n=7, gonadectomy)groups in each sex. Left carotid artery was not injured with 2.0 F PTCA balloon until estradiol was injected for three days. Rats were killed 2 wk after injury. Neointimal areas and media area, ratios of intimal areas/media areas were measured with computer. Results Male neointimal areas and ratios of intimal areas /media areas in estradiol group were less than those in intact control group significantly(all P0.05). Conclusions Estrdiol inhibits neointimal proliferation after the gonadectomy in rats undergoing carotid artery balloon injury.