V.hollisae.V.parahaemolyticus could cause septicemia.The results of infectious ability of the 4 dominant vibrios showed that vibrios could cause the wound inflammatory of mice.Inflammatory cells were found subcutanously and in the striped muscles,forming into cellulites.Conclusion: The characters of bacteria spectrum in Southeast China Sea area has been investigated.The virulence and the wound infection ability of the 4 dominant vibrios have been tested in mice.

Objective To explore the clinical value of changes of serum thyroid function and immune content from patients with gestational hyperthyroidism.Methods A total of 146 cases of patients with hyperthyroidism during pregnancy and 73 cases of patients with recurrence of hyperthyroidism after delivery were chosen as the experimental group,and 73 cases of patients without recurrence of hyperthyroidism after delivery were chosen as the control group.The thyroid function and thyroid-stimulating antibody (TSAb)concentrations at different period of pregnancy for two groups were tested.The thyroid function and TSAb concentrations after pregnancy of the two groups were also tested.Results At the mid and late period of pregnancy,the concentrations of free triiodothyronine (FT3)and free tetraiodothyronine (FF4) were significantly lower than early pregnancy.The concentrations of hypersensitive thyroid stimulating hormone (sTSH) and TSAb were increased gradually.The concentrations of FT3,FT4,and TSAb after pregnancy were all significantly higher than control group (P ＜ 0.05).Conclusions FT3,FT4,sTSH,and TSAb can be used for early diagnosis of hyperthyroidism during pregnancy,and have a certain predictive value for relapse after secretion.

Base on the situations of urine analysis at home and abroad and the wrong tendency of neglecting the urine formed elements in routine test, the authors describe the clinical value and standard test protocol according to the national guideline, references, personal clinical practice and research findings. The authors also make comments on the advantages and disadvantages of microscopic analysis with a variety of instruments, and provide suggestions on how to strengthen the quality management of urine analysis in our couutry.

Objective:To investigate the serum endothelial cell specific molecule-1 (ESM-1) level and its clinical significance in patients with hypertensive disorders complicating pregnancy (HDCP).Methods:Three hundred pregnant women with HDCP (HDCP group) and 300 pregnant women undergoing health check-ups (healthy control group) from January to December 2019 in Maternity and Child Health Hospital of Wenling City of Zhejiang Province were selected. In HDCP group, hypertension during pregnancy was in 94 cases, mild preeclampsia was in 131 cases, and severe preeclampsia was in 75 cases. The serum ESM-1, serum uric acid and 24-hour urine protein levels were detected. The incidences of neonatal asphyxia, fetal distress, cesarean section, polyhydramnios, premature rupture of membranes and postpartum hemorrhage in the HDCP group were recorded.Results:The serum ESM-1, serum uric acid and 24-hour urine protein in HDCP group were significantly higher than those in healthy control group: (0.42 ± 0.05) μg/L vs. (0.33 ± 0.04) μg/L, (262.34 ± 23.18) mmol/L vs. (212.37 ± 22.16) mmol/L and (0.36 ± 0.07) g vs. (0.11 ± 0.03) g, and the differences were statistical significant ( P<0.01). The correlation analysis results showed that the serum ESM-1 was positive correlation with serum uric acid and 24-hour urine protein in HDCP pregnant women ( r = 0.497 and 0.512, P<0.01). The serum ESM-1 in pregnant women with mild preeclampsia and severe preeclampsia were significantly higher than that in pregnant women with hypertension during pregnancy: (0.44 ± 0.05) and (0.51 ± 0.06) μg/L vs. (0.38 ± 0.04) μg/L, the serum ESM-1 in pregnant women with severe preeclampsia was significantly higher than that in pregnant women with mild preeclampsia, and the differences were statistical significant ( P<0.05). With the median serum ESM-1 (0.41 μg/L) as the boundary, pregnant women with HDCP were divided into high ESM-1 group (154 cases) and low ESM-1 group (146 cases). The incidences of neonatal asphyxia, fetal distress, cesarean section, polyhydramnios, premature rupture of membranes and postpartum hemorrhage in high ESM-1 group were significantly higher than those in low ESM-1 group: 14.94% (23/154) vs. 6.16% (9/146), 26.62% (41/154) vs. 9.59% (14/146), 46.10% (71/154) vs. 29.45% (43/146), 25.32% (39/154) vs. 3.42% (5/146), 23.38% (36/154) vs. 4.11% (6/146) and 20.13% (31/154) vs. 7.53% (11/146), and the differences were statistical significant ( P<0.01). Conclusions:Serum ESM-1 level is elevated in pregnant women with HDCP . Serum ESM-1 level is closely related to the severity of HDCP and pregnancy outcomes.

Objective To investigate the main species of marine bacteria and their distribution characteristics in China seas. Methods Seawater samples were obtained from sea water about one meter below the sea level along the navigation course, and then the bacteria therein were enriched, cultured, identified and tested for drug sensitivity. Results A total of 528 seawater samples were collected from four seas of China, and 759 marine bacteria in 145 species were isolated. The isolates were mainly Vibro, Enterobacteriaceae, Nonfermenter, Fungi, Pasteurella, Gram positive cocci, Eikenella corrodens and Anaerobic bacteria. Vibrio accounted for 52.9% of the 759 strains of marine bacteria, among which Vibrio alginolyticus, Vibrio fluvialis and Vibrio parahaemolyticus accounted for 75%. There was no significant difference in the quantity of Vibrio alginolyticus, Escherichia coli and Vibrio parahaemolyticus between the 4 sea areas (P=0.071). Chi-square test showed that significant differences existed in the distribution of seven species of marine bacteria among the 4 China seas (P=0.0004). The Gram-positive cocci were isolated more often in Bohai than from other seas; Eikenella corrodens were detected mostly in Yellow Sea; Vibrio were the predominant bacteria in East China sea, up to 70.8%; more Fungi were found in South China sea. The main features of specific bacteria isolated from the four sea areas was higher number of species with less quantity. From North to South, Enterococcus faecalis, Flavobacterium, Vibrio carchariae and C. famata were found to constitute the highest number. Conclusions In China seas, Vibrios are the dominant bacteria, and the numbers of Anaerobic bacteria and Gram-positive cocci are extremely low. There is a significant difference in the distribution of marine bacteria among 4 China seas.

Objective:To do research on prediction method of medical equipment sudden fault for the requirement of medical security at sea which is away from base.Methods: Sudden fault data of the medical equipment is a random variable which often manifest as the time of sudden fault happening. The possible distribution type of fault data is hypothesized according to the engineering experience. In order to ensure the sudden fault density function of medical equipment, parameter estimation and distribution fit test are carried out for the fault data.Results: The sudden fault prediction model of medical equipment is established to get the future sudden fault probability of medical equipment based on the distribution function of fault data.Conclusion: The results of case analysis validate the rationality of sudden fault prediction model.

Objective: To observe the change of blood cell,marker of vascular endothelium and lung of rabbits during experimental severe decompression sickness(DCS) and to compare each parameter between survival group and dead group.Methods: 14 rabbits were put into the chamber.They stayed for 35mins under 0.55 MPa,and another 35 mins under 0.7 MPa,then decompressed to 0.1 MPa in 4 minutes.Samples were drew before the compression and after decompression for testing count of white blood cell(WBC),neutrophilic leukocyte,lymphocyte and platelet(PLT) and the concentration of ET-1 and the vWF.The tissue of lung of all the rabbits was observed under light and electron microscope.The changes of above parameters of survival rabbits were compared with that of the dead.Results: After rapid decompression,there were 8 rabbits died within 30 minutes,while the other 6 rabbits survived without any symptom of decompression sickness after 24 hours observation.Before the compression,the total count of WBC of dead rabbits was?(11.13?2.37)?10~(9)/L,which was higher the that of survival rabbits,(8.87?2.11)?10~(9)/L.After decompression,the decrease of PLT of dead group,(273?(63)?)10~(9)/L,was more than that of survival group,(148?63)?10~(9)/L.Ttissuedamage of lungs of both dead and survived rabbits was observed as partial alveoli collapse,interstitial edema,local emphysema,various emboli in microcirculation and thickening of respiratory membrane.The damage of lung of dead rabbits was much worse than that of the survival.Conclusion:Experimental DCS induced the increasing of circulation tension,WBC obturation,various emboli,thickening of respiratory membrane,consumption of surface active substance in the body of rabbits.Those rabbits who reacted too severe and had bad compensation ability would die,on the contrary,those rabbits who had a good compensation could survive.

Objective To investigate the role of Stat1 in pathological process of nerve cells apoptosis induced by diffuse axonal injury (DAI) on rats. MethodsThe DAI model was established by using an injury model adapted from Marmarou et al. in 1994. All animals were divided into three groups, including control group, mock group and test group sacrificed on 6, 12, 24, 48, 72, 120 and 240 hours post injury (hpi). The paraffin-embedded sections of brain tissue were processed for HE staining and Bielschowsky’s silver method. Cell apoptosis was examined by flow cytometry and the expression of bax and bcl-2 were analyzed by RT-PCR. And Phospho-Ser727 Stat1 expression was examined by immunohistochemistry in different brain regions. ResultsThere was no brain contusion within HE staining, however, waving and enlargement of axons were observed within Bielschowsky’s silver method. The apoptotic rate of brain cells as well as PCR products ratio of bax to bcl-2 was highest at 24 hpi and decreased with time. An up-regulation of Phospho-Ser727 Stat1 at 6 hpi was discernible, and then reached the top at 24 hpi in cortex, cerebellum, brain stem and corpus callosum, and at 12 hpi in hippocampus. This increase was associated with the nerve cells apoptosis, r=0.921. In addition, the Phospho-Ser727 Stat1 positive cells were neurons and glial cells assessed from morphous. ConclusionsOur data indicate that Stat1 may contribute to the apoptosis of DAI on rats. In addition, the expression of Phospho-Ser727 Stat1 in glial cells suggested that glial cells may play an important role in the pathogenic mechanism of DAI.

Objective: To analyze chromosome aberrations in bladder transitional cell carcinoma with exfoliated cells, and to evaluate the clinical value of fluorescence in situ hybridization (FISH) in bladder cancer. Methods: FISH was performed using centromeric probes of 3, 7, 17 and locus probe of p16 to examine chromosome aberrations of exfoliated cells of 56 bladder cancer patients and 20 healthy controls to analyze the correlation of chromosome aberration with the pathological features of bladder cancer. The urine cytology of the 56 bladder cancer patients was performed. Results: The rates of aneuploidy of chromosomes 3, 7, and 17 were 58.9%, 39.3%, 58.9% and 75.0% for aberration of p16 in exfoliated cells from the 56 bladder cancer patients. All of the aberrations had no correlation with tumor stage (P>0.05). The aberrations of chromosomes 3, 7, and 17 were significantly correlated with pathological grade (P<0.05). The sensitivity of the 4 chromosome probes for detecting bladder cancer was 80.4%. The detection rate of FISH was obviously higher than that of udne cytology. Conclusion: Chromosome aberration is correlated with the growth of bladder cancer. The detection of FISH has significance for early di-agnosis, prognosis evaluation, and recurrence monitoring of bladder cancer.

AIM　To study the chemical constituents from the mycelia of Hypomyces sp. METHODS Silica gel column chromatography was employed for the isolation and purification. The structure of compound 1 was elucidated on the basis of spectral analysis. RESULTS and CONCLUSION　A new perylenequinone, named hypomycin B 1, was isolated from the mycelia of Hypomyces sp.