Acute ischemic stroke is characterized by high morbidity,high mortality,and high disability.At present,thrombolytic therapy is the only treatment that can improve the prognosis of patients in acute phase.This article reviews the choice of time window,the primary means of thrombolytic therapy and medications,and the complications of recanalization after thrombolytic therapy.

Objective To investigate the relation between macrophage infiltration and degree of calcific aortic stenosis (CAVS)in elder patients.Methods The aortic valve specimens were collected from 2012 September to 2014 March in Depart‐ment of Cardiac Surgery of Hainan General Hospital ,for operation removal ,from 80 cases[40 males and 40 females ,age:(58 ± 6)years old];normal aortic valve specimens were collected from 7 patients undergoing surgical removal of the normal aortic valve because of aneurysm[4 males ,3 female patients ,aged(41 ± 8)years] ,and 80 healthy adults served as control group.The clinical data of patients were collected ,and CAVS valve and normal valve were observed by HE staining.Expression feature of macrophages was studied by immunohistochemical(IHC)staining.IPP(Image‐Pro Plus)software was used to measure the densi‐ty of macrophages ,and Pearson relation analysis was employed to study the correlation between the density of macrophages and the degree of valve stenosis.Results As compared with healthy adults ,cholesterol and C reactive protein in patients with CAVS were significantly elevated.There were infiltration of inflammatory cells ,new blood capillaries and calcification in the valves of CAVS lesions observed by HE staining.No positive expression of macrophage marker CD68 was found in normal aortic valves , but CD68 positive expression was observed in the valves of CAVS by IHC staining ,and mainly concentrated in the peripheral calcification and osteoid tissues.Simple linear regression analysis showed the density of macrophages was positively correlated with the large pressure gradient(r=0.75 ,P<0.05) ,the average differential pressure(r=0.75 ,P<0.05) ,and the maximum aortic jet velocity(r=0.72 ,P<0.05) ,however ,was negatively correlated(r= -0.71 ,P<0.05)with the valve orifice area .Con‐clusion Compared with healthy adult volunteers ,the concentration of cholesterol and C reactive protein were elevated in blood of the patients with CAVS.Compared with the normal valve ,the macrophages were infiltrated in CAVS valves ;the density of macrophage was positively correlated with the degree of CAVS.

Objective To evaluate the impact of triglyceride(TG) on the severity and the extent of coronary artery stenosis.Methods Ninety-three cases of young patients(below 45 years old, with or without hypertriglyeeridemia)underwent pereutaneous coronary intervention(PCI)were retrospectively analyzed.The patients were divided into three groups according to TG levels:I group(TG<1.70 mmol/L,36 cases),II group(1.70 mmol/L≤TG≤2.25 mmol/L,19 cases),III group(TG>2.25 mmol/L,38 cases).The severity of coronary artery stenosis and the extent Was compared among the three groups.Results In all patients, the male and the smoker occupied the overwhelming majority[94.62%(88/93),83.87%(78/93)].Total cholesterol(TC),non-high density lipoprotein cholesterol(HDL-C)in III group were higher than those in I group with statistical significance(P=0.006,0.003).There were no significant difference among the three groups in low density lipopmtein cholesterol(LDL-C),HDL-C,fasting blood glncose(P=0.648,0.795,0.247).There were no significant difference among the three groups in extent of coronary artery and severity of coronary artery stenosis(P=0.241,0.879).Conclusions There is no significant difference among the three groups in severity of coronary artery stenosis and extent of coronary artery.Hypertriglyeeridemia ian't the determiner on severity of coronary heart disease.

Objective To analyze the occurrence of sharp injuries among health care workers(HCWs),and put forward suggestions for safety management of sharp inj uries.Methods Recorded form of sharp inj uries reported in a hospital in August 2006-July 2015 were analyzed retrospectively,relevant data were analyzed statistically.Results A total of 211 sharp injuries occurred among HCWs were reported,sharp injuries mainly occurred among nurses(n=118,55.92%)and doctors(n=83,39.34%);117(55.45%)sharp injuries occurred in HCWS whose length of service was≤5 years;the main occurrence locations of sharp injuries were surgical ward(n=67,31.75%),internal medicine ward(n=65,30.81%),and outpatient as well as emergency departments(n=33,15.64%);the main de-vices causing sharp injuries were syringe needle(n=91,43.13%),suture needle(n=34,16.11%),and scalp nee-dle/transfusion needle(n=27,12.80%);the main injury-related manipulations were all types of injection(n=40, 18.95%),intravenous transfusion (n=38,18.00%),and surgical suturing/cutting (n=36,17.06%).The main opportunities of injuries was during manipulation(treatment,examination,operation,n=109,51.66%).Among bloodborne pathogens contaminated devices,contaminated by HBV,HCV,and HIV were 95 (76.61%),25 (20.16%),and 4 cases(3.23%)respectively.There was no bloodborne pathogen infection occurred.155(73.46%) of 211 HCWs were inoculated hepatitis B vaccine.Conclusion Prevention of sharp injuries should be focused on nurses and doctors who work for≤5 years,HCWs in surgical ward,internal medicine ward,and outpatient as well as emergency departments.Prevention of sharp inj uries during manipulations such as inj ection,intravenous transfu-sion,suturing/cutting should be intensified.Related hospital departments should improve the reporting process and intensify follow-up.

Alzheimers disease (AD) is a progressively and retrogressively neurodegenerative disorder characterized by two hallmarks: senile plaques (SP) and neurofibrillary tangles (NFTs). The studies of animal models, which are crucial to the further study of AD, will promote the understanding of the etiology, mechanisms and medicine screening. Although the animal models such as experimental injury, normal aging and compound types could represent the cognitive deficit, they lack the essential neuropathological characteristics of AD. Senescence accelerated mouse / prone 8 is one of relatively practical and stable models. The most enchanting focus of AD models is transgenic mice that provide new carriers for the research of pathogenic forms of specific genes and metabolites thereof in vivo. As therapies of AD evolve from symptomatic treatment to causative treatment,so must the animal models of AD.

DNA, Neoplasm ; analysis ; Female ; Flow Cytometry ; Humans ; Hydatidiform Mole ; diagnosis ; genetics ; In Situ Hybridization, Fluorescence ; Microsatellite Repeats ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Pregnancy ; Triploidy ; Uterine Neoplasms ; diagnosis ; genetics

Objective To investigate the risk of myocardial damage induced by obstructive sleep apneahypopnea syndrome (OSAHS) in children. Methods Twenty-three children who were in-hospital patients and diagnosed as adenoidal hypertrophy or palatine tonsil hypertrophy enrolled in the study as cases,among whom 15cases were affected by OSAHS (OSAHS group) ,and 8 cases were not affected by OSAHS( no-OSAHS group).Twenty-seven healthy children from health physical examination were enrolled as controls (control group).Chemiluminescence was used to detect the value of Troponin Ⅰ (TnⅠ) in all children, and the values of the three groups were compared. Results In the OSAHS group, TnⅠ was (0. 022 ± 0. 009) μg/L;in the non-OSAHS group, TnⅠ was (0.035 ± 0.012) μg/L;in the control group,TnⅠ was (0.017 ± 0.015) μg/L. There were significant difference among the three groups ( F = 8. 369, P = 0. 001 ). Furthermore, compared with the control group,TnI in the OSAHS was significantly higher( P ＜ 0. 001 ). Conclusion Children with adenoid or tonsil hypertrophy associated with OSAHS in children were found to have myocardial damage compared to healthy children, whereas similar on myocardial damage to children with adenoid and tonsil hypertrophy but not OSAHS.

Animals ; Cell Separation ; methods ; Female ; Male ; Myocytes, Cardiac ; cytology ; metabolism ; Proto-Oncogene Proteins c-kit ; metabolism ; Rats ; Rats, Sprague-Dawley ; Stem Cells ; cytology ; metabolism

Objective To study the changes of system immune and intestinal immune in the progression of non-alcoholic fatty liver disease due to obesity. Methods Ninty male SD rats were divided into control, high-sucrose and high-fat diet groups. Non-alcoholic fatty liver disease models were established by feeding with high-sucrose diet or high-fat diet and were killed at the 4th,8th and 12th weeks with 10 each for each group. The extent of liver steatosis was observed with HE staining.Portal blood endotoxin level was assessed by limulus test. The percentage of CD4+ and CD8+ cells in peripheral blood mononuclear cells (PBMC) and lymphocytes in Peyer's patches (PP) were calculated by flowcytometry. Results In comparison with control group, the endotoxin level was not elevated from the 4th week to 12th week in high-sucrose diet group, (all P values＞0.05), but was increased in high-fat diet group at the 8th week (P＜0.05). CD4/CD8 ratio in PBMC was higher in high-sucrose and higt-fat diet groups than that in control group at the 4th week (P＜0. 05) ,but was lower than that in control group at the 8th and 12th weeks (P＜0. 05). Whereas the variation of CD4/CD8 ratio in PP was consisted with that in PBMC between the high-sucrose and high-fat diet groups at the 4 th and 8 th weeks, but there was no difference when compared with control group at the 12 th week (P＞0.05).Conclusion Obesity can inhibit systematic immune and intestinal immune. The intestinal immune may be regulated by the liver.

Interleukin (IL)-27 is a new member of the IL-6/IL-12 family composed of p28 subunit and Epstein-Barr virus induced gene 3 (EBI3) subunit. Its receptor is composed of WSX-1 and gp130. It has dual properties including pro-inflammatory and anti-inflammatory function at different conditions. Studies have shown that IL-27 exerts its biological activities through stimulating JAK1/STAT1, JAK1/STAT3 signal pathways and regulating the production of Th1, Th17 as well as their related-cytokines. Furthermore, IL-27 can exert the role of anti-tumor activity by enhancing the effect of cytotoxic T cells and anti-angiogenesis.