1.Expression of beta-catenin in human colorectal adenoma and carcinoma.
Qiong HUANG ; Yi-min ZHU ; Xiao-ming XING ; Mao-de LAI
Journal of Zhejiang University. Medical sciences 2004;33(2):121-124
OBJECTIVETo investigate the expression of beta-catenin and its significance in colorectal neoplasms.
METHODSTissue specimens of normal colorectal mucosa, mucosa adjacent to carcinoma, colorectal adenoma and adenocarcinoma were examined for beta-catenin with immunohistochemistry.
RESULTSBeta-catenin was mainly expressed in the cytomembrane of normal mucosa and mucosa adjacent to cancer (the positive rates were 94.6% and 86.5%, respectively) and also in the cytoplasm (the positive rates were 38.7% and 55.0%, respectively), while its expression was negative in the cell nucleus. In adenoma and adenocarcinoma, beta-catenin was mainly expressed in the cytoplasm (the positive rates were 85.1%,and 93.7%, respectively) and partially in the cell nucleus (the positive rates were 12.8% and 23.4%, respectively). Compared with normal mucosa and mucosa adjacent to cancer, the expression of beta- catenin in the cytomembrane of adenoma and adenocarcinoma was significantly lower (P<0.05), while its expression in the cytoplasm and cell nucleus of adenoma and adenocarcinoma was significantly higher (P<0.05). The positive rates of cytoplasm in highly-and moderately differentiated adenocarcinoma were significantly higher than that in poorly-differentiated adenocarcinoma (the positive rates were 100%, 95.5% and 68.8%, respectively). Beta-catenin expression rate in cytoplasm was correlated with Dukes'stages of adenocarcinoma, which was significantly lower in stage A than in stage B/C.
CONCLUSIONThe expression of beta-catenin is significantly correlated with differentiation and Dukes'stages of colorectal carcinoma and it can be used as an indicator for the prognosis of colorectal carcinoma.
Adenocarcinoma ; chemistry ; pathology ; Adenoma ; chemistry ; pathology ; Colorectal Neoplasms ; chemistry ; pathology ; Cytoplasm ; chemistry ; Cytoskeletal Proteins ; analysis ; Humans ; Immunohistochemistry ; Prognosis ; Trans-Activators ; analysis ; beta Catenin
2.Apoptosis and Proliferation in Paired Primary Colorectal Adenocarcinomas and Their Liver Metastases.
Jinsil SEONG ; Jae Ho CHO ; Woo Ik YANG ; Eun Ji CHUNG ; Nam Kyu KIM
Yonsei Medical Journal 2004;45(2):187-192
The proliferation potentials and the level of apoptosis were compared in paired primary colorectal adenocarcinomas and their liver metastases within each individual. From a total of 22 patients 44 specimens of paired primary and metastatic tumors were obtained for analysis. The levels of spontaneous apoptosis (a spontaneous apoptosis index, SAI: % apoptotic nuclei among a total of 1000 nuclei) and of proliferation (KI-67 index: % positively stained cells for KI-67 among a total of 1000 cells) were analyzed between primary and metastatic tumors. Survival rates and its relationship with the clinical parameters were also analyzed. The overall survival rate at 5 years was 16.9% with the median survival time of 45 months. T-stage (p=0.005) and time to liver metastasis (synchronous versus metachronous, p=0.03) showed statistical significance in relation to survival. The mean SAI of primary tumors was 1.35 +/- 0.25, which was not statistically different from the 1.58 +/- 0.18 of metastatic tumors (p=0.33). The mean KI-67 indices in primary and metastatic tumors were 23.9 +/- 3.4 and 16.4 +/- 2.5, respectively, and this difference was statistically significant (p=0.016). Subset analysis showed significant difference in the KI-67 index in the synchronous group but not in the metachronous group. No significant difference was shown in the relative ratios of apoptosis to proliferation between the primary tumor and the metastasis within each individual. The results in this study may partly explain the indolent behavior of liver metastasis from colorectal cancer and provides a rationale for the active treatment of metastatic tumors as well as of primary disease.
Adenocarcinoma/chemistry/*secondary
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Adult
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Aged
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*Apoptosis
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Cell Division
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Colorectal Neoplasms/chemistry/*pathology
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Female
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Human
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Liver Neoplasms/chemistry/*secondary
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Male
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Middle Aged
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Support, Non-U.S. Gov't
3.Research development of L1-CAM(CD171)in human cancer.
Chao ZHANG ; Yu FAN ; Li FU
Chinese Journal of Pathology 2013;42(8):574-576
Animals
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Cell Adhesion
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Cell Movement
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Colorectal Neoplasms
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metabolism
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pathology
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Drug Delivery Systems
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Epithelial-Mesenchymal Transition
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Humans
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Lung Neoplasms
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metabolism
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pathology
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Neoplasm Invasiveness
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Neoplasms
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metabolism
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pathology
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Neural Cell Adhesion Molecule L1
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chemistry
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metabolism
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Pancreatic Neoplasms
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metabolism
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pathology
4.A novel method for detecting circulating tumor cells immunity based on micro-nano technique.
Shuting LI ; Shufen JIAO ; Yu LI ; Yujuan WU ; Rongyun ZHAI ; Zhe WANG ; Jing CHENG ; Weiying ZHANG ; Yali BEN
Chinese Journal of Biotechnology 2023;39(9):3849-3862
This study was to develop a new method for detecting circulating tumor cells (CTCs) with high sensitivity and specificity, therefore to detect the colorectal cancer as early as possible for improving the detection rate of the disease. To this end, we prepared some micro-column structure microchips modified with graphite oxide-streptavidin (GO-SA) on the surface of microchips, further coupled with a broad-spectrum primary antibody (antibody1, Ab1), anti-epithelial cell adhesion molecule (anti-EpCAM) monoclonal antibody to capture CTCs. Besides, carboxylated multi-walled carbon nanotubes (MWCNTs-COOH) were coupled with colorectal cancer related antibody as specific antibody 2 (Ab2) to prepare complex. The sandwich structure consisting of Ab1-CTCs-Ab2 was constructed by the microchip for capturing CTCs. And the electrochemical workstation was used to detect and verify its high sensitivity and specificity. Results showed that the combination of immunosensor and micro-nano technology has greatly improved the detection sensitivity and specificity of the immunosensor. And we also verified the feasibility of the immunosensor for clinical blood sample detection, and successfully recognitized detection and quantization of CTCs in peripheral blood of colorectal cancer patients by this immunosensor. In conclusion, the super sandwich immunosensor based on micro-nano technology provides a new way for the detection of CTCs, which has potential application value in clinical diagnosis and real-time monitoring of disease.
Humans
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Nanotubes, Carbon/chemistry*
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Neoplastic Cells, Circulating/pathology*
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Biosensing Techniques
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Immunoassay/methods*
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Antibodies
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Colorectal Neoplasms/diagnosis*
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Electrochemical Techniques/methods*
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Gold/chemistry*
5.Cyclo-oxygenase-2 and p53 Immunoreactivity in Superficial Early Colorectal Carcinoma.
You Sun KIM ; So Dug LIM ; Jin Kwang LEE ; Seong Eun KIM ; Soo Hyung RYU ; Jung Whan LEE ; Jeong Seop MOON
The Korean Journal of Gastroenterology 2006;47(5):350-356
BACKGROUND/AIMS: De novo colorectal carcinoma shows more aggressive behavior including submucosal invasiveness. Both p53 and cyclo-oxygenase-2 (COX-2) have been shown to be involved in colon carcinogenesis, progression from adenoma to carcinoma, and submucosal invasion by tumor. We performed this study to evaluate the expression of p53 and COX-2 protein in de novo carcinoma, compared with ex-adenoma carcinoma. METHODS: Twenty three flat adenomas, 19 ex-adenoma carcinomas, 6 de novo carcinomas were included in this study. The expression of p53, COX-2 and Ki-67 were examined immunohistochemically. RESULTS: Both ex- adenoma carcinomas and de novo carcinomas showed similar size and shape. Positive staining for p53 was detected in 3 of 23 (13%) flat adenomas, in 11 of 19 (57.8%) ex-adenoma carcinomas (p<0.05), and in 1 of 6 (16.6%) de novo carcinomas. Increased numbers of COX-2 positive tumor cells were observed in 1 of 23 (4.3%) flat adenomas, in 2 of 19 (10.5%) ex-adenoma carcinomas, and in 3 of 6 (50%) de novo carcinomas. COX-2 positive expression showed increased tendency in de novo carcinoma (p=0.073). There was no correlation between COX-2, p53, and Ki-67 expression. CONCLUSION: De novo carcinoma shows increased tendency of COX-2 expression, but decreased p53 expression when compared to ex-adenoma carcinoma. These immunohistochemical findings are in accordance with the fact that de novo carcinoma has no preceding adenoma, with more frequent submucosal invasion despite the small lesion size.
Adenoma/chemistry/pathology
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Carcinoma/chemistry/pathology
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Colorectal Neoplasms/chemistry/*pathology
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Cyclooxygenase 2/*analysis
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Female
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Humans
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Immunohistochemistry
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Ki-67 Antigen/analysis
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Male
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Middle Aged
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Tumor Markers, Biological/analysis
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Tumor Suppressor Protein p53/*analysis
6.Expression of tissue inhibitor of metalloproteinase-1 in colorectal carcinoma and its clinical implications.
Yang LIU ; Bo JIANG ; Hua-sheng TONG ; Xiao-rong LAI
Journal of Southern Medical University 2006;26(5):699-700
OBJECTIVETo investigate the expression and clinical implication of tissue inhibitor of metalloproteinase-1 (TIMP-1) in colorectal carcinoma.
METHODSTIMP-1 expression in 54 colorectal carcinoma was observed by SP immunohistochemical method, and the results were analyzed in relation to the clinical data of patients.
RESULTSTIMP-1 was localized on the membrane and in the cytoplasm of the enteric epithelial cells, and its expression rate was 100% in normal tissue but only 59.6% (31/52) in colorectal carcinoma tissues. In addition, the expression rate of TIMP-1 was higher in the tumor tissues without lymph node metastasis than in tissues with lymph node metastasis (P<0.05).
CONCLUSIONThe expression of TIMP-1 is inversely correlated to lymph node metastasis of colorectal carcinoma, and decreased TIMP-1 expression may play a role in the progression of colorectal carcinoma.
Adult ; Aged ; Colorectal Neoplasms ; metabolism ; pathology ; Epithelial Cells ; chemistry ; pathology ; Female ; Humans ; Immunohistochemistry ; Lymphatic Metastasis ; Male ; Middle Aged ; Tissue Inhibitor of Metalloproteinase-1 ; biosynthesis
7.Expression of HLA-DR antigen in large bowel carcinoma.
Eun Sook CHANG ; Soo Sang SOHN
Journal of Korean Medical Science 1995;10(5):334-341
One hundred large bowel carcinomas were studied immunohistochemically with regard to expression of HLA-DR antigen (DR). One or two sections from each tumor including surrounding normal mucosa were examined by a semiquantitative counting system for tumor cells and mucosal and stromal infiltrates of lymphocytes and mononuclear cells (MNCs) with DR expression and the results were applied Chi-square test. The rate of presence of DR positive (DR+) lymphocytes in lymphoid nodules and DR+ lymphocytes/ MNC in the adjacent mucosa and stroma in DR+ carcinoma (50%) was higher (P < 0.01) than in DR- carcinoma (21.9%). Thirty-six carcinomas (36%) were DR+. Three (75%) out of 4 DR+ poorly differentiated carcinomas and six (20%) out of 30 DR+ moderately differentiated carcinomas showed homogeneously strong DR+ expression. There was tendency for poorly differentiated carcinoma to be more homogeneous DR+ expression. According to Dukes' stage, four (80%) out of 5 carcinomas in Dukes' stage D were DR-. An increased infiltration of lymphocytes/MNCs into adjacent mucosa and stroma in large bowel carcinomas is possibly related with DR expression by carcinoma. From the results of this study, we postulated as follows: 1) DR+ tumor cells may act as antigen-presenting cells, 2) They may have an inhibitory effect for distant metastasis, 3) Poorly differentiated carcinoma expressed more DR+ homogeneously.
Adult
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Aged
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Antibodies
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Colorectal Neoplasms/blood/*chemistry/pathology
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Epithelium/chemistry
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Female
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HLA-DR Antigens/*analysis
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Human
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Immunohistochemistry
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Leukocytes, Mononuclear/chemistry
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Lymphocytes/chemistry
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Male
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Middle Age
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Neoplasm Staging
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Support, Non-U.S. Gov't
8.The construction of yeast two-hybrid method in the protein-interactions and its significance in hepatic metastasis of colorectal carcinoma.
Fu-Yi ZUO ; Shi-Yong LI ; Ping AN ; Bo YU ; Hui-Yun CAI
Chinese Journal of Surgery 2004;42(11):672-674
OBJECTIVETo construct the yeast two-hybrid system, and screen the proteins which interact with FasL, and investigate the relationship of FasL and hepatic metastasis of colorectal carcinoma.
METHODSWe have cloned the FasL gene into the pGBKT7 vector as the bait, then screened the fetal liver cDNA library, and have got a series of specific proteins that interact with FasL protein. Using the bioinformatics, we analyzed the interacting proteins in the mechanism of hepatic metastasis of colorectal carcinoma.
RESULTSWe have screened several proteins that interaction with FasL protein, including metallothionein 1K, 1G, 2A, cathepsin B, fatty acid synthase, interferon alpha-inducible protein 27, phospholipid scramblase, Ser/Thr-like kinase, anchor attachment protein, fibulin-5.
CONCLUSIONSWe have successfully constructed the yeast two-hybrid system, and preliminary identified that the interaction between FasL, metallothionein, cathepsin and anchor attachment protein is radically related to the hepatic metastasis of colorectal carcinoma.
Cathepsin B ; metabolism ; Cloning, Molecular ; Colorectal Neoplasms ; chemistry ; pathology ; Fas Ligand Protein ; Gene Library ; Humans ; In Vitro Techniques ; Liver Neoplasms ; chemistry ; secondary ; Membrane Glycoproteins ; genetics ; metabolism ; Metallothionein ; metabolism ; Protein Binding ; Tumor Necrosis Factors ; genetics ; metabolism ; Two-Hybrid System Techniques ; Yeasts ; genetics
9.Significance of detecting disseminated tumor cells in peripheral blood of gastric and colorectal cancer patients.
Xi-wei ZHANG ; Ping FAN ; Hong-yu YANG ; Li YANG ; Guo-yu CHEN
Chinese Journal of Oncology 2003;25(1):66-69
OBJECTIVETo evaluate the clinical significance of CK20 mRNA expression in detecting disseminated tumor cells in peripheral blood of gastric and colorectal cancer patients.
METHODSExpression of CK20 mRNA was investigated by RT-PCR in bone marrow, portal vein and peripheral blood in 47 gastric, 58 colorectal cancer patients and 6 non-cancer volunteers. All the patients were followed-up for one year.
RESULTSThere was no positive expression of CK20 mRNA in 6 non-cancer volunteers. The positive rates of CK20 mRNA in bone marrow, portal vein were 87.2% (41/47) and 85.1% (40/47) in gastric cancer, and were 77.6% (45/58) and 74.1% (43/58) in colorectal cancer. The positive rates of CK20 mRNA in peripheral blood in gastric and colorectal cancer patients were 42.6% (20/47) and 44.8% (26/58) by one single test, and were 74.5% (35/47) and 69.0% (40/58) by two tests. The overall positive rate of CK20 mRNA in peripheral blood (two tests) was similar to that in bone marrow and portal vein. The overall positive rate of CK20 mRNA in peripheral blood was higher in two tests than in one single test (P < 0.05) and in advanced than early lesions. The relapse rate within one year was higher in CK20 mRNA positive patients than the negative ones (P < 0.05).
CONCLUSIONDetection of cancer cells by RT-PCR for CK20 mRNA in peripheral blood, being as sensitive and specific as in bone marrow and portal vein, is reliable and convenient in diagnosing micrometastasis of gastric and colorectal cancer, which possesses clinical significance in assessing the prognosis and scheme of therapy.
Adolescent ; Adult ; Aged ; Biomarkers, Tumor ; blood ; Colorectal Neoplasms ; blood ; pathology ; Female ; Humans ; Intermediate Filament Proteins ; blood ; genetics ; Keratin-20 ; Male ; Middle Aged ; Neoplasm Metastasis ; Neoplastic Cells, Circulating ; chemistry ; RNA, Neoplasm ; blood ; Reverse Transcriptase Polymerase Chain Reaction ; Stomach Neoplasms ; blood ; pathology
10.Variations of cellular membrane phospholipids with genesis and hepatic metastasis of large intestine cancer.
Shiyong LI ; Bo YU ; Ping AN ; Zhenjia LIANG ; Shujun YUAN ; Huiyun CAI
Chinese Journal of Surgery 2002;40(8):561-563
OBJECTIVETo separate and detect membrane phospholipids and study the relationship of metabolism and signal transduction pathways of membrane phospholipids with genesis and hepatic metastasis of large intestinal carcinoma.
METHODSForty-eight cases of colorectal cancer were detected with high performance liquid chromatography. Membrane phospholipids of phosphatidylinosital (PI), phosphatidylserine (PS), phosphatidylethanolamine (PE) and phosphatidylcholine (PC) in primary foci, paratumor intestinal mucosa and hepatic metastasis of large intestine cancer were separated and analyzed.
RESULTSIn primary foci, paratumor intestinal mucosa, and hepatic metastasis of the 48 cases, the contents (mg/g) of PI were: 0.92 +/- 0.12, 1.57 +/- 0.14, 1.54 +/- 0.15 respectively, and PC 56.47 +/- 5.33, 108.57 +/- 6.37, 116.35 +/- 6.85. The contents of PI and PC were higher in primary foci and hepatic metastasis than in paratumor mucosa (F = 363.10, 870.10, P < 0.01). The contents of PE in the three tissues were 18.23 +/- 3.56, 42.02 +/- 4.33, 79.51 +/- 5.52, and in hepatic metastasis was the highest (F = 1 149.63, P < 0.01). PI and PC in primary foci of hepatic metastatic group and nonmetastasis group were not significantly different (t = 3.55, P > 0.05). But the PE content was higher in hepatic metastasis than in primary foci (t = 115.87, P < 0.01).
CONCLUSIONSMembrane phospholipids have obvious variations in genesis and hepatic metastasis of large intestine cancer. Rises of PI and PC were associated with genesis of large intestine carcinoma. The increase of PE content is closely related to invasion and hepatic metastasis of large intestine cancer.
Adult ; Aged ; Aged, 80 and over ; Chromatography, High Pressure Liquid ; Colorectal Neoplasms ; metabolism ; pathology ; Female ; Humans ; Intestinal Mucosa ; chemistry ; Liver Neoplasms ; secondary ; Male ; Membrane Lipids ; analysis ; Middle Aged ; Phospholipids ; analysis