PURPOSE: The aim of this study was to investigate the change of collagen content and the subtype of collagen in compensated and decompensated detrusors by benign prostatic hyperplasia (BPH). MATERIALS AND METHODS: A total of 62 patients with BPH who underwent transurethral resection of the prostate (TUR-P) was involved in this study. Preoperatively, the Watts factor (WF) was measured for detrusor contractile strength, and the patients were divided into 3 groups according to these measurements: group 1 consisted of patients with decreased contractile strength, group 2 consisted of patients with normal contractile strength, and group 3 consisted of patients with increased contractile strength. The chips of the detrusor were obtained at the time of TUR-P. Total collagen concentration was quantified by hydroxyproline assay. Immunohistochemical staining was performed for the expression and localization of type I and III collagen, and Western blot analysis was performed for the extent of expression of type I and III collagen. RESULTS: The total collagen concentrations of groups 1, 2, and 3, which significantly varied among the three groups, were 132.5+/-59.2, 265.1+/-96.8, and 437.6+/-162.5mug/mg, respectively (ANOVA test, p<0.05). In immunohistochemical staining and Western blot analysis, type I collagen was expressed similarly among the three groups. The expression of type III collagen was weakest in group 1 and strongest in group 3. There was a positive correlationship between WF and the expression of type III collagen in the detrusor layer. CONCLUSIONS: These data suggest that the change of total content and subtype of collagen in the detrusor muscle is the major histological change in BPH and type III collagen, which play an important role in detrusor contractility.
Blotting, Western ; Collagen Type I ; Collagen Type III ; Collagen* ; Humans ; Hydroxyproline ; Prostate ; Prostatic Hyperplasia* ; Urinary Bladder

Absorbable atelo-collagen sponge (TERUPLUG(R), Termo Co. Tokyo, Japan) is inserted in the extraction wound where alveolar bone is exposed. It protects wounds and promotes the formation of granulation. This is made of atelo-collagen, to minimize antigenicity, which is cross-linked by heat treatment for biocompatibility. TERUPLUG(R) consists of between 85 and 95 % of collagen type I and between 5 to 15 % of collagen type III. The raw material for the collagen is derived from bovine skin. It features a sponge block design and is shaped for easy insertion in the extraction wound. This study was designed to find out the bone healing capacity of TERUPLUG(R). We implanted TERUPLUG(R) (experimental group I) and TERUPLUG(R) with rhBMP-2 (experimental group II) in the rabbit cranium defect and then histologically analysed the specimen. The results were as follows. 1. In the 4 weeks, a lot of the newly formed collagen fibers around material of the experimental group I implanted TERUPLUG(R) were observed. But, in the experimental group II implanted TERUPLUG(R) with rhBMP-2, a little of newly formed collagen fibers around material were observed. The cell proliferating activity and apoptosis of the experimental group I, II was positive in and around the implanted material. 2. In the 8 weeks, the amount of newly formed and matured bone in the experimental group II was more observed than the experimental group I and control group. The results of this study indicate that absorbable atelo-collagen sponge (TERUPLUG(R)) is relatively favorable bone void filler with biocompatibility and has the better bone healing capacity in case of application with rhBMP-2.
Apoptosis ; Collagen ; Collagen Type I ; Collagen Type III ; Hot Temperature ; Porifera ; Skin ; Skull ; Tokyo

Absorbable atelo-collagen sponge (TERUPLUG(R), Termo Co. Tokyo, Japan) is inserted in the extraction wound where alveolar bone is exposed. It protects wounds and promotes the formation of granulation. This is made of atelo-collagen, to minimize antigenicity, which is cross-linked by heat treatment for biocompatibility. TERUPLUG(R) consists of between 85 and 95 % of collagen type I and between 5 to 15 % of collagen type III. The raw material for the collagen is derived from bovine skin. It features a sponge block design and is shaped for easy insertion in the extraction wound. This study was designed to find out the bone healing capacity of TERUPLUG(R). We implanted TERUPLUG(R) (experimental group I) and TERUPLUG(R) with rhBMP-2 (experimental group II) in the rabbit cranium defect and then histologically analysed the specimen. The results were as follows. 1. In the 4 weeks, a lot of the newly formed collagen fibers around material of the experimental group I implanted TERUPLUG(R) were observed. But, in the experimental group II implanted TERUPLUG(R) with rhBMP-2, a little of newly formed collagen fibers around material were observed. The cell proliferating activity and apoptosis of the experimental group I, II was positive in and around the implanted material. 2. In the 8 weeks, the amount of newly formed and matured bone in the experimental group II was more observed than the experimental group I and control group. The results of this study indicate that absorbable atelo-collagen sponge (TERUPLUG(R)) is relatively favorable bone void filler with biocompatibility and has the better bone healing capacity in case of application with rhBMP-2.
Apoptosis ; Collagen ; Collagen Type I ; Collagen Type III ; Hot Temperature ; Porifera ; Skin ; Skull ; Tokyo

PURPOSE: The aim of this study was to determine differences in the specific collagen types of the transverse carpal ligament (TCL) in patients with carpal tunnel syndrome (CTS) and a control group without CTS. MATERIALS AND METHODS: Surgical specimens from 19 dissected TCLs in patients with idiopathic CTS and 5 controls without CTS were taken (1x1 cm2). We analyzed the manifestations of collagen types I and III by immunohistochemical staining. RESULTS: We found a homogeneous manifestation of type I and III collagens in the control group. However, in CTS patients, type III collagen was sporadically found around the perivascular and pericellular area, and type I collagen showed no definite differences between the two groups. The cellularity in CTS patients was much lower than that in the control group. CONCLUSION: The TCL in CTS patients shows reduced manifestation of type III collagen and less cellularity. These intrinsic changes may play a role in the different manifestations of collagen types in TCL.
Carpal Tunnel Syndrome* ; Collagen Type I ; Collagen Type III ; Collagen* ; Humans ; Ligaments*

This study was focused on the localizations or expressions of fibronectin and interactions of fibronectin with type I and type III collagen in the new forming tissues during wound healing in rat skin. Adult male rats(SPrague-Dawley strain), 150-200 gm weight, were used for experimental animals. And the experimental animals were divided into 3 or 4 groups by passed days after incision of back skin.The specimens were obtained and immunohistologically stained for electron microscopy from each of groups and the specimens were observed with the electron microscope (Hitach-600 Model). The results obtained were as follows. 1. Strong positive reactions for type I collagen are seen in the new forming tissue at day 3 after incision of skin, and moderate reactions at day 5 and day 7 after incision of skin. 2. The weak reactions for type III collagen are seen at day 3 and day 5 after incision in the new forming tissues of skin. And the type III collagen reaction at day 7 are more increased than that of other groups. 3. Fibronectin expressions are seen moderately at day 5 and day 3 groups, and strong at day 5 group, but at day 7 group reactions are rapidly decreased. It is suggested that type I and III collagens are gradually increased from the beginning of wound healing to the time of the new fibrous tissues formation. The great activities of the fibronection are seen within processes of the new tissue formation for wound healing events.
Adult ; Animals ; Collagen ; Collagen Type I ; Collagen Type III ; Fibronectins ; Humans ; Male ; Microscopy, Electron ; Rats ; Skin ; Wound Healing ; Wounds and Injuries

Photorefractive keratectomy(PRK) was performed on 60 rabbit eyes, and synthetic inhibitor of metalloproteinase(SIMP) and cyclosporin A(CsA) was topically administered and their effects on coreneal haze were evaluated. They were randeomized to one of four groups: group A received topical SIMP, group B received topical CsA, group C received both SIMP and CsA, and group D received vehicles. At one, two, four, and six weeks after surgery, slit lamp examination was performed, and haze gradings were recorded. Light microscopy, together with immunohistochemistry using antibodies against collagen types III, IV, and VI were performed in corneas from all groups. Slit lamp examination and light microscopy revealed that SIMP significantly reduced corneal haze after PRK and subepithelial deposition of newly synthesized extracellular matrix, but CsA did not. By immunohistochemistry, deposition of types III and IV collagen was noted in ablated area of all groups. Positive staining for type III collagen was less frequent in groups treated with SIMP than in groups not treated with SIMP. In conclusion, SIMP significantly reduced the synthesis of type III collagen in treated area as well as corneal haze after excimer laser PRK in rabbits.
Antibodies ; Collagen Type III ; Collagen* ; Cornea ; Cyclosporine* ; Extracellular Matrix ; Immunohistochemistry ; Lasers, Excimer* ; Microscopy ; Photorefractive Keratectomy* ; Rabbits*

OBJECTIVE: To explore the healing effect of low intensity pulsed ultrasound with three different timing of intervention after tenotomy of Achilles tendon in rats. METHOD: One hundred and thirty-two male rats were divided into experimental and control groups. Both groups were classified according to treatment phase: treatment on inflammatory phase (group I), proliferative phase (group II), and maturation phase (group III). Each groups were divided into 2 subgroups according to tendon excision time: 1 day after 7 consecutive treatment on 3 different phases (I-A, II-A, and III-A) and 30 days after tenotomy (I-B, II-B, and III-B). Three MHz pulsed ultrasound was administered on right tendon for 4 mins at 0.5 W/cm2. The excised tendons of all groups were compared histologically and biochemically as control. RESULTS: The tendons of II-A experimental group revealed increased fibroblasts. The collagen fibers in the neo-tendon of II-B and III-B experimental groups had a tendency to be arrayed more regularly. On I-A group, the neo-tendon showed high immunoreactivity for type I and particularly type III collagen in cytoplasm of fibroblasts and collagen fibers. The imunoreactivity for type III collagen in the neo-tendon of II-A experimental group increased than control. The concentration of collagen of the neo-tendon was significantly increased on I-A and II-A experimental groups compared with control (p<0.05). Collagen concentration of the neo-tendon of II-B experimental group increased significantly compared with control and I-B and III-B experimental groups (p<0.05). CONCLUSION: These results suggest that low intensity pulsed ultrasound therapy on injured Achilles tendon may be of benefit such as increasing collagen synthesis in the early healing process, especially in proliferative phase.
Achilles Tendon* ; Animals ; Collagen ; Collagen Type III ; Cytoplasm ; Fibroblasts ; Humans ; Male ; Rats* ; Tendons ; Tenotomy ; Ultrasonography*

We performed photorefractive keratectomy(PRK) on 10 rabbit eyes and determined the distribution of collagen type III, IV VI, VII at postoperative 2, 4 and 6 months to examine immunohistochemical changes after PRK. Type III collagen was not found in the normal cornea but strongly detected in the regenerated corneal stroma at all intervals. It was most prominent at 2 months after surgery and then decreased. Type IV collagen was detected in basement membrane in both normal and ablated corneas at all intervals and the staining was more intense in ablatd corneas than in normal cornea. There was no difference of staining intensity among the groups of different intervals. Type IV collagen was found in both normal and healed corneal stroma at all intervals and there was no difference of staining intensity between normal and ablated corneas and among the groups of different intervals. Type VII collagen was observed as a linear continuous band along the basal surface of epithelium in normal cornea. At 2 months after surgery, type VII collagen staining in basement membrane zone became denser than normal cornea, but segmented. At 4 months after surgery, continuous band of collagen type VII staining was observed, but it was less intense than in normal cornea. At 6 months after surgery, the intensity of continuous band of collagen type VII was the same as in normal cornea. This results suggest that the presence of type III collagen in the regenerated cornea may be related to the development of postoperative subepithelial opacity after PRK and the normalization of collagen type IV and VII at postoperative 6 months may mean the complete reestablished of the adhesion of regenerated epithelium and stroma.
Basement Membrane ; Collagen Type III* ; Collagen Type IV ; Collagen Type VII ; Collagen* ; Cornea* ; Corneal Stroma ; Epithelium ; Immunohistochemistry ; Lasers, Excimer* ; Photorefractive Keratectomy

BACKGROUND: Changes in the composition of the extracellular matrix (ECM) occur between the proliferating and involuted phases of infantile hemangiomas (IH), and are associated with angiogenic growth. We examined the composition of the ECM in proliferating and involuted IHs and assessed correlations between the composition of the ECM and whether the IH was in the proliferating or the involuted phase. METHODS: We evaluated IH samples from a cohort of patients who had five proliferating IHs and five involuted IHs. The following ECM molecules were analyzed using enzyme-linked immunosorbent assays and immunohistochemistry: laminin, fibronectin, collagen type I, collagen type II, and collagen type III. RESULTS: The involuted IHs had higher levels of deposition of collagen type III than the proliferating IHs. The median values (interquartile ranges) were 1.135 (0.946-1.486) and 1.008 (0.780-1.166) (P=0.019), respectively. The level of laminin was higher in involuted IHs than in proliferating IHs, with median values (interquartile ranges) of 3.191 (2.945-3.191) and 2.479 (1.699-3.284) (P=0.047), respectively. Abundant collagen type III staining was found in involuted IHs. Laminin alpha4 chain staining was clearly present within the basement membrane adjacent to the blood vessels, and was significantly more intense in involuted IHs than in proliferative IHs. CONCLUSIONS: Involuted hemangiomas showed extensive deposition of collagen III and laminin, suggesting that differences in the composition of the ECM reflect stages of the development of IHs. This pattern may be due to the rapid senescence of IHs.
Aging ; Basement Membrane ; Blood Vessels ; Cohort Studies ; Collagen ; Collagen Type I ; Collagen Type II ; Collagen Type III ; Enzyme-Linked Immunosorbent Assay ; Extracellular Matrix* ; Fibronectins ; Hemangioma* ; Humans ; Immunohistochemistry ; Laminin

Allografts or autografts of bone-tendon unit have been used for ligament injuries or deficiencies after limb salvage operation for malignant bone tumors around joints. While the remodeling process of the ligament or tendon of the allograftor autograft and the microscopic and biomechanical changes of tendon-bone graft interface have been widely investigated, little is known about the ultrastructural and biochemical changes of the transitionall zone in tendon-bone junction. This study was performed to analyzed the ultrastructural and biochemical changes of the transitional zone after tendon-bone auto-and allo-grafting. A total of twenty four rabbits were divided into two group. In 12 animals(allograft group), two Achilles ten-don-bone unit per each animal were taken and transplanted to another rabbit after preservation below -70degrees C for 2 weeks. In the other 12 animals(autograft group), the Achilles tendon-bone unit was harvested from one side and transplanted to the other side of the rabbit. After operation, their legs were immobilized with short leg cast for 4 weeks, and then mobilized freely. Four animals in each group were sacrificed at four, eight and sixteen-weeks after the transplantation, and their grafted Achilles tendon-bone interfaces were used for analysis of the ultrasturctural and biochemical changes. The following results were obtained. 1. Histologically new cartilage cells were observed at postoperative 16 weeks with local presence of faint tidemark in the autograft group but not in the allograft group. Complete remodeling of the transitional zone had not observed histologically in both groups. 2. Ultrasturctural analysis revealed no definite differences between both groups. However, the slightly rapid appearance of parallelism and cross-striation of microfibrils in the autograft group and time-related restoration of fibers and fibroblasts were observed. 3. From biochemical analysis, type I collagen concentratin was increased, and an early rapid increase of type III collagen and glycosaminoglycan were also observed. In conclusion, these data suggested that type III collagen and glycosaminoglycan are important in stabilization of grafted tendon-bone unit, especially in the transitional zone. The histological and biochemical changes in allograft group were relatively similar to that of autograft group although the allograft group showed the delayed pattern of remodeling. Therefore, the tendon- allograft could be used as a good substitute for the autograft for tendon or ligament reconstruction when no suitable autograft is available.
Allografts ; Animals ; Autografts ; Cartilage ; Collagen Type I ; Collagen Type III ; Fibroblasts ; Joints ; Leg ; Ligaments ; Limb Salvage ; Microfibrils ; Rabbits* ; Tendons ; Transplants*