No abstract available.
Bone Regeneration* ; Collagen Type I* ; Rabbits*

The present study has been undertaken to pursue the cytotoxic effects of cis-Platin on the osteoid formation in metaphysis of rat tibia. By using the immunohistological staining method for type I collagen in rat tibial osteoid, the author detected the deposition of type I collagen, which is the collagenous constituent of endochondral osteoid, after administration of cis-Platin in experimental animals. For the immunological reactions of type I collagen, we used the rabbit anti-rat collagen type I polyclonal antibody as primary antibody and biotinylated goat anti-rabbit IgG as secondary antibody. The distributions of immunohistological reactions in the each of metaphyseal osteoids were analyzed with an image analyzer, and we studied the variances of type I collagens by statistical probabilities. In 12 hours after cis-Platin injection, immunoreactive area in the osteoid of metaphysis was distinctly decreased. Immunoreactive area of type I collagen in osteoids of 1 day and 3 days group metaphysis was increased more than that of 12 hours group and the type I collagen in the metaphysis showed weak immunoreactions of type I collagens with an image analyzer. In the osteoids of 7 days group after cis-Platin injection, the immunoreactive area was similar to that of control group. It is consequently suggested that cis-Platin would induce the decrease of type I collagen in the osteoid. But the type I collagen in tibial osteoid shows the increase from a few days after cis-Platin injection.
Animals ; Collagen ; Collagen Type I* ; Goats ; Immunoglobulin G ; Rats* ; Tibia*

PURPOSE: The aim of this study was to investigate the change of collagen content and the subtype of collagen in compensated and decompensated detrusors by benign prostatic hyperplasia (BPH). MATERIALS AND METHODS: A total of 62 patients with BPH who underwent transurethral resection of the prostate (TUR-P) was involved in this study. Preoperatively, the Watts factor (WF) was measured for detrusor contractile strength, and the patients were divided into 3 groups according to these measurements: group 1 consisted of patients with decreased contractile strength, group 2 consisted of patients with normal contractile strength, and group 3 consisted of patients with increased contractile strength. The chips of the detrusor were obtained at the time of TUR-P. Total collagen concentration was quantified by hydroxyproline assay. Immunohistochemical staining was performed for the expression and localization of type I and III collagen, and Western blot analysis was performed for the extent of expression of type I and III collagen. RESULTS: The total collagen concentrations of groups 1, 2, and 3, which significantly varied among the three groups, were 132.5+/-59.2, 265.1+/-96.8, and 437.6+/-162.5mug/mg, respectively (ANOVA test, p<0.05). In immunohistochemical staining and Western blot analysis, type I collagen was expressed similarly among the three groups. The expression of type III collagen was weakest in group 1 and strongest in group 3. There was a positive correlationship between WF and the expression of type III collagen in the detrusor layer. CONCLUSIONS: These data suggest that the change of total content and subtype of collagen in the detrusor muscle is the major histological change in BPH and type III collagen, which play an important role in detrusor contractility.
Blotting, Western ; Collagen Type I ; Collagen Type III ; Collagen* ; Humans ; Hydroxyproline ; Prostate ; Prostatic Hyperplasia* ; Urinary Bladder

The purpose of this experiment was to examine the histological changes and the pattern of expression of type I, II collagen in the elongated area by distraction osteogenesis in the rabbit mandible. Sixteen rabbits weighing 2.5kg-3kg were used for this experiment. Experimental group was distracted at the rate of 0.7mm, twice/day for 7days, and control group was only osteotomized. After 5 days latency, osteotomic site is distracted for 7days. Consolidation period is 28days. The animal was sacrificed at the 3rd, 7th, 14th, 28th day after the operation. The distracted bone was examined by histological analysis and RT-PCR analysis. The results were summarized as follows: 1. Experimental group was observed that the gaps between the distracted bone edges were occupied by new bone. 2. Expression of Type I collagen were detected throughout the experiment in both groups and Expression of Type I collagen were markedly increased during distraction and consolidation period in experimental group than control group. 3. Expression of Type II collagen were detected throughout the experiment in both groups and expression of Type II collagen were maintained at high level during distraction and consolidation period in experimental group than control group. From these results, in contrast to type II collagen, type I collagen seemed to be more expressed by mechanical stimuli during distraction and consolidation period. The predominent mechanism of new bone formation in the distraction gap was intramembranous bone formation, but some of the regenerated bone was formed by endochondral ossification.
Animals ; Collagen ; Collagen Type I ; Collagen Type II* ; Mandible* ; Osteogenesis ; Osteogenesis, Distraction* ; Rabbits

Absorbable atelo-collagen sponge (TERUPLUG(R), Termo Co. Tokyo, Japan) is inserted in the extraction wound where alveolar bone is exposed. It protects wounds and promotes the formation of granulation. This is made of atelo-collagen, to minimize antigenicity, which is cross-linked by heat treatment for biocompatibility. TERUPLUG(R) consists of between 85 and 95 % of collagen type I and between 5 to 15 % of collagen type III. The raw material for the collagen is derived from bovine skin. It features a sponge block design and is shaped for easy insertion in the extraction wound. This study was designed to find out the bone healing capacity of TERUPLUG(R). We implanted TERUPLUG(R) (experimental group I) and TERUPLUG(R) with rhBMP-2 (experimental group II) in the rabbit cranium defect and then histologically analysed the specimen. The results were as follows. 1. In the 4 weeks, a lot of the newly formed collagen fibers around material of the experimental group I implanted TERUPLUG(R) were observed. But, in the experimental group II implanted TERUPLUG(R) with rhBMP-2, a little of newly formed collagen fibers around material were observed. The cell proliferating activity and apoptosis of the experimental group I, II was positive in and around the implanted material. 2. In the 8 weeks, the amount of newly formed and matured bone in the experimental group II was more observed than the experimental group I and control group. The results of this study indicate that absorbable atelo-collagen sponge (TERUPLUG(R)) is relatively favorable bone void filler with biocompatibility and has the better bone healing capacity in case of application with rhBMP-2.
Apoptosis ; Collagen ; Collagen Type I ; Collagen Type III ; Hot Temperature ; Porifera ; Skin ; Skull ; Tokyo

Absorbable atelo-collagen sponge (TERUPLUG(R), Termo Co. Tokyo, Japan) is inserted in the extraction wound where alveolar bone is exposed. It protects wounds and promotes the formation of granulation. This is made of atelo-collagen, to minimize antigenicity, which is cross-linked by heat treatment for biocompatibility. TERUPLUG(R) consists of between 85 and 95 % of collagen type I and between 5 to 15 % of collagen type III. The raw material for the collagen is derived from bovine skin. It features a sponge block design and is shaped for easy insertion in the extraction wound. This study was designed to find out the bone healing capacity of TERUPLUG(R). We implanted TERUPLUG(R) (experimental group I) and TERUPLUG(R) with rhBMP-2 (experimental group II) in the rabbit cranium defect and then histologically analysed the specimen. The results were as follows. 1. In the 4 weeks, a lot of the newly formed collagen fibers around material of the experimental group I implanted TERUPLUG(R) were observed. But, in the experimental group II implanted TERUPLUG(R) with rhBMP-2, a little of newly formed collagen fibers around material were observed. The cell proliferating activity and apoptosis of the experimental group I, II was positive in and around the implanted material. 2. In the 8 weeks, the amount of newly formed and matured bone in the experimental group II was more observed than the experimental group I and control group. The results of this study indicate that absorbable atelo-collagen sponge (TERUPLUG(R)) is relatively favorable bone void filler with biocompatibility and has the better bone healing capacity in case of application with rhBMP-2.
Apoptosis ; Collagen ; Collagen Type I ; Collagen Type III ; Hot Temperature ; Porifera ; Skin ; Skull ; Tokyo

PURPOSE: The aim of this study was to determine differences in the specific collagen types of the transverse carpal ligament (TCL) in patients with carpal tunnel syndrome (CTS) and a control group without CTS. MATERIALS AND METHODS: Surgical specimens from 19 dissected TCLs in patients with idiopathic CTS and 5 controls without CTS were taken (1x1 cm2). We analyzed the manifestations of collagen types I and III by immunohistochemical staining. RESULTS: We found a homogeneous manifestation of type I and III collagens in the control group. However, in CTS patients, type III collagen was sporadically found around the perivascular and pericellular area, and type I collagen showed no definite differences between the two groups. The cellularity in CTS patients was much lower than that in the control group. CONCLUSION: The TCL in CTS patients shows reduced manifestation of type III collagen and less cellularity. These intrinsic changes may play a role in the different manifestations of collagen types in TCL.
Carpal Tunnel Syndrome* ; Collagen Type I ; Collagen Type III ; Collagen* ; Humans ; Ligaments*

No abstract available.
Collagen Type I* ; Skin* ; Wound Healing* ; Wounds and Injuries*

In the present study, we evaluated the effect of CGM on osteogenic differentiation of cultured osteoblasts, and determined whether combination treatment with LLLT had synergistic effects on osteogenic differentiation. The results indicated that CGM promoted proliferation, differentiation, and mineralization of osteoblasts at the threshold concentration of 10 µg/ml; whereas, CGM showed cytotoxic properties at concentrations above 100 µg/ml. ALP activity and mineralization were increased at concentrations above 10 µg/ml. CGM in concentrations up to 10 µg/ml also increased the expression of osteoblast-activated factors including type I collagen, BMP-2, RUNX2, and Osterix. The CGM (50 µg/ml) and LLLT (80 mW for 15 sec) combination treatment group showed the highest proliferation levels, ALP activity, and mineralization ratios. The combination treatment also increased the levels of phosphorylated forms of p38, ATF2, PKD, ERK, and JNK. In addition, the osteoblast differentiation factors including type I collagen, BMP-2, RUNX2, and Osterix protein levels were clearly increased in the combination treatment group. These results suggested that the combination treatment of CGM and LLLT has synergistic effects on the differentiation and mineralization of osteoblastic cells.
Collagen Type I ; Gingiva* ; Low-Level Light Therapy* ; Miners ; Osteoblasts*

BACKGROUND: In contrast with bone formation markers, most of available indices of bone resorption are urine markers and show relatively high degree of variability. The serum resorption assay has therefore been developed. We evaluated serum bone-derived degradation products of type I collagen C-telopeptide (s-CTX) and serum osteocalcin by Elecsys 2010 (Hitachi Boehringer Mannheim, Tokyo, Japan). METHODS: For 18 healthy controls, 15 osteopenic and 7 osteoporotic patients samples, serum CTX and serum osteocalcin were measured by Elecsys 2010 using -CrossLaps/serum (Roche Diagnostic Corp., Indianapolis, USA) kit and N-MID Osteocalcin (Roche Diagnostic Corp. kit, respectively. DPD by Immulite (Diagnostic Products Corp., LA, USA) using Pyrilinks-D(TM) (Diagnostic Products Corp.) kit and serum osteocalcin for correlation by Gamma counter (Hewlett Packard, Meriden, USA) using ELSA-OSTEO (CIS, Cedex, France) kit were measured. RESULTS: The within-run and between-run coefficient of variation (CV) values of s-CTX were 6.41% and 6% in low concentrations and 3.84% and 7% in high concentrations, respectively. The within-run and between-run CV values of serum osteocalcin were 2.21% and 6% in low concentrations and 1.25% and 3% in high concentrations, respectively. The dilution recovery of s-CTX and serum osteocalcin was 100-169% (mean, 134%) and 80-138% (mean, 104%), respectively. S-CTX and DPD (R=0.369, P=0.019), and serum osteocalcin by Elecsys 2010 and RIA (R=0.889, P<0.001) showed positive correlations, respectively. CONCLUSTIONS: S-CTX and serum osteocalcin by Elecsys 2010 exhibits good analytical performance and correlate with DPD and serum osteocalcin by RIA, respectively. Therefore, these may replace DPD and serum osteocalcin by RIA and can be used for bone resorption and formation markers, respectively.
Bone Resorption ; Collagen Type I ; Humans ; Osteocalcin* ; Osteogenesis