1.ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera.
Hye Jin AHN ; Sera KIM ; Dae Yong KIM ; Ho Woo NAM
The Korean Journal of Parasitology 2003;41(3):175-177
An ELISA was established to measure bovine IgG directed against the recombinant antigenic determinant of Nc-p43, a major surface antigen of Neospora caninum. In a previous study, two thirds of the Cterminal of the molecule was expressed as a 6 x His tagged protein (Ncp43P) for ELISA using 2/3 of the N-terminal of SAG1 from Toxoplasma gondii as a control (TgSAG1A). Among 852 cattle sera collected from stock farms scattered nation-wide, 103 sera (12.1%) were found to react with Ncp43P positively, but no positive reaction was observed with TgSAG1A. This study shows that Ncp43P could be available as an efficient antigen for the diagnosis of neosporosis in cattle. Furthermore, it together with TgSAG1A, could be useful for the differential diagnosis of N. caninum and T. gondii infections in other mammals.
Animals
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Antibodies, Protozoan
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Cattle
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Cattle Diseases/diagnosis/epidemiology
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Coccidiosis/diagnosis/epidemiology/veterinary
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Enzyme-Linked Immunosorbent Assay/methods/*veterinary
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Immunoglobulin G/*analysis
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Neospora/*immunology
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Protozoan Proteins/*immunology
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Seroepidemiologic Studies
2.Review of Neospora caninum and neosporosis in animals.
The Korean Journal of Parasitology 2003;41(1):1-16
Neospora caninum is a coccidian parasite of animals. It is a major pathogen for cattle and dogs and it occasionally causes clinical infections in horses, goats, sheep, and deer. Domestic dogs are the only known definitive hosts for N. caninum. It is one of the most efficiently transmitted parasite of cattle and up to 90% of cattle in some herds are infected. Transplacental transmission is considered the major route of transmission of N. caninum in cattle. Neospora caninum is a major cause of abortion in cattle in many countries. To elicit protective immunity against abortion in cows that already harbor a latent infection is a major problem. This paper reviews information on biology, diagnosis, epidemiology and control of neosporosis in animals.
Animals
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Buffaloes/parasitology
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Coccidiosis/diagnosis/epidemiology/transmission/*veterinary
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Disease Models, Animal
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Dogs
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Female
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Goats/parasitology
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Horses/parasitology
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Humans
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Neospora/*physiology
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Pregnancy
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Prevalence
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Sheep/parasitology