1.An improved BALB/c 3T3 cell transformation assay and its application in the cocarcinogenesis study.
Lei ZHANG ; Rui ZHAO ; Ru-gang ZHONG ; Ling ZHOU ; De-chang LI ; Yong-ning WU ; Yi ZENG
Chinese Journal of Experimental and Clinical Virology 2009;23(2):121-123
OBJECTIVETo improve the protocol of BALB/c 3T3 cell transformation assay, and apply it to the cocarcinogenesis study.
METHODAppropriate serum concentration, culture media and method of administration were selected by testing their effects on the growth and transformation of BALB/c 3T3 cells. The co-carcinogenic activity between diethylnitrosamine (DEN) and microcystin-LR (MC-LR) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) were examined using the improved cell transformation assay. The malignant characteristics of transformed cells were verified by neoplasia in SCID mice.
RESULTSThere were strong co-carcinogenic activity between DEN and TCDD. On the contrary, although MC-LR has strong ability to induce cell transformation, the effect was markely inhibited by DEN. The transformed cells show some malignant characteristics.
CONCLUSIONThe improved BALB/c 3T3 cell transformation assay is reliable and time-saving, and can be efficiently used in the study of cocarcinogenesis.
3T3 Cells ; Animals ; BALB 3T3 Cells ; Biological Assay ; methods ; Carcinogens ; toxicity ; Cell Transformation, Neoplastic ; chemically induced ; Cocarcinogenesis ; Male ; Mice ; Mice, SCID ; Random Allocation
2.Different modifying responses of capsaicin in a wide-spectrum initiation model of F344 rat.
Ja June JANG ; Kyung Ja CHO ; Yon Sil LEE ; Jong Hee BAE
Journal of Korean Medical Science 1991;6(1):31-36
The modifying potential of capsaicin (CAP) on lesion development was examined in a rat multiorgan carcinogenesis model. Groups 1 and 2 were treated sequentially with diethylnitrosamine (DEN) (100 mg/kg, ip, single dose at commencement), N-methylnitrosourea (MNU) (20 mg/kg, ip, 4 doses at days 2, 5, 8, and 11), and N,N-dibutylnitrosamine (DBN) (0.05% in drinking water during weeks 3 and 4). Group 3 received vehicles without carcinogens during the initiation period. Group 4 served as the untreated control. After this initiating procedure, Groups 2 and 3 were administered a diet containing 0.01% CAP. All surviving animals were killed 20 weeks after the beginning of the experiment and the target organs examined histopathologically. The induction of GST-P+ hepatic foci in rats treated with carcinogens was significantly inhibited by treatment with CAP. CAP treatment significantly decreased the incidence of adenoma of the lung but increased the incidence of papillary or nodular (PN) hyperplasia of the urinary bladder. The tumor incidence of other organs, such as the kidney and thyroid, was not significantly different from the corresponding controls. These results demonstrated that concurrent treatment with CAP not only can inhibit carcinogenesis but can also enhance it depending on the organ. Thus, this wide-spectrum initiation model could be used to confirm organ-specific modification potential and, in addition, demonstrate different modifying effects of CAP on liver, lung, and bladder carcinogenesis.
Animals
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Capsaicin/pharmacology/*toxicity
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Cocarcinogenesis
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Diethylnitrosamine
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Liver Neoplasms, Experimental/chemically induced/prevention & control
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Lung Neoplasms/chemically induced/prevention & control
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Male
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Methylnitrosourea
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Neoplasms, Experimental/*chemically induced/prevention & control
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Nitrosamines
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Rats
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Rats, Inbred F344
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Urinary Bladder Neoplasms/chemically induced
3.Alteration of p53 gene during tree shrews' hepatocarcinogenesis.
Jian-jia SU ; Yuan LI ; Ke-chen BAN ; Liu-liang QIN ; Hui-yun WANG ; Chun YANG ; Chao OU ; Xiao-xian DUAN ; Yong-yi LI ; Rui-qi YAN
Chinese Journal of Hepatology 2003;11(3):159-161
OBJECTIVETo detect the expression and variation of p53 gene during tree shrews' hepatocarcinogenesis induced by hepatitis B virus (HBV) and aflatoxin B1 (AFB1).
METHODSTree shrews were divided into four groups: the tree shrews were infected with HBV and fed with AFB1 in group A, only infected with HBV in group B, fed with AFB1 alone in group C, and normal control in group D. All the tree shrews were performed liver biopsy every 15 weeks. The tissues of liver and tumor were detected by immunohistochemistry and molecular biotechnologies.
RESULTS(1) The incidence of hepatocellular carcinoma (HCC) in group A (66.7%) was higher than that in Group B and C (30%). HCC appearance in group A was earlier than that in group C (120.0 weeks +/-16.6 weeks vs 153.3 weeks +/-5.8 weeks, t = 3.336, P<0.01). (2) Mutated p53 protein was not found before the 75th week of the experiment in each group. (3) At the 105th week, the expression rates of mutated p53 protein were 78.6%, 60% and 71.4% in group A, B and C respectively, which were much higher than that (10%) in group D (x2 > or = 5.03, P<0.05). An abnormal band of p53 gene was detected in both group A and C. (4) The mutation points of p53 gene in liver cancer of tree shrew were at codon 275, 78 and 13. The nucleotide sequence and amino acids sequence of tree shrew's wild-type p53 showed 91.7% and 93.4% homology with those of human p53 respectively.
CONCLUSIONSThere is a remarkable synergistic effect between HBV and AFB1 on HCC. Mutated p53 protein is expressed before HCC occurrence, which promotes the development and progress of HCC. HBV and AFB1 may synergistically induce p53 gene mutation.
Aflatoxin B1 ; toxicity ; Animals ; Carcinoma, Hepatocellular ; genetics ; Cocarcinogenesis ; Gene Expression Regulation, Neoplastic ; Genetic Variation ; Hepatitis B ; virology ; Hepatitis B virus ; Liver Neoplasms, Experimental ; genetics ; Point Mutation ; RNA, Neoplasm ; analysis ; Tumor Suppressor Protein p53 ; genetics ; Tupaiidae
4.Changes of expression of miR-155 in colitis-associated colonic carcinogenesis.
Weiwei LI ; Wenxiao HAN ; Xinhua ZHAO ; Hongying WANG
Chinese Journal of Oncology 2014;36(4):257-262
OBJECTIVETo investigate the changes of miR-155 and its target genes in colitis-associated carcinogenesis.
METHODSColitis-associated colon cancer was induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in C57BL/6 mice. Mice of three different stages during the development of colon cancer were obtained, named AD1, AD2 and AD3, respectively. A control group of mice without any treatment and a DSS only group representing chronic inflammation without cancer were set up as well. Colon tissue was collected and expression of miR-155 in the colon tissues was measured by real-time fluorescent quantitative PCR. TargetScan and PicTar were used to predict potential target genes of miR-155, which were then preliminarily screened with our gene expression microarray database of AOM-DSS mouse model. Regular PCR was used to confirm the changes of the expression of these potential target genes in AOM-DSS mouse model.
RESULTSColitis-associated colon cancer was effectively induced by azoxymethane and dextran sulfate sodium in C57BL/6 mice. Histological examination revealed that the evolution process was sequentially from normal, mild dysplasia, moderate dysplasia, and severe dysplasia to adenocarcinoma in the AOM-DSS mouse model. The level of miR-155 was gradually elevated with the formation of colitis-associated colon cancer. There was no significant difference between the levels of miR-155 expression in the DSS group (0.005 6 ± 0.003 7) and control group (0.012 0 ± 0.005 1) (P > 0.05), but the level of miR-155 in the AD3 group (0.054 4 ± 0.027 0) was significantly higher than that of the DSS group (0.005 6 ± 0.003 7)(P < 0.01). No significant change of miR-155 expression was found in the DSS only group. The relative expression levels of miR-155 in the control group, DSS only group and AD3 group were 0.012 0 ± 0.005 1, 0.005 6 ± 0.003 7, 0.054 4 ± 0.027 0, respectively. Data analysis with the gene expression microarray showed that Tle4, Kcna1, Itk, Bcorl1, Cacna1c, Rspo2 and Foxo3 were potential target genes of miR-155 in the AOM-DSS mouse model. Changes of Kcna1 and Cacna1c in the AOM-DSS mouse model were validated to be consistent with the changes obtained with the gene expression microarray.
CONCLUSIONThe up-regulation of miR-155 is related to colitis-associated carcinogenesis, but is irrelevant to chronic inflammation in the mouse model.
Adenocarcinoma ; chemically induced ; genetics ; metabolism ; Animals ; Azoxymethane ; toxicity ; Carcinogens ; toxicity ; Cocarcinogenesis ; Colitis ; chemically induced ; genetics ; metabolism ; Colon ; metabolism ; Colonic Neoplasms ; chemically induced ; genetics ; metabolism ; Dextran Sulfate ; toxicity ; Gene Expression Profiling ; Male ; Mice ; Mice, Inbred C57BL ; MicroRNAs ; metabolism ; Precancerous Conditions ; chemically induced ; genetics ; metabolism ; Up-Regulation