No abstract available.
Clonorchis sinensis*

The survival ability of the larve of Clonorchis sinensis in raw fish dish prepared by traditional culinary method in NghiaLac commune, NghiaHung District,NamDinh province and the larve of Paragonomus heterotrimus in grilled crabs processed in KhanhHoa commune, LucYen district, YenBai province was investigated..In this raw fish dish, 93-95% of the microcerca of Clonorchis sinensis and in this grilled crab, 23,3-65% of microcerca had been living
Fishes ; Clonorchis sinensis ; epidemiology

A survey concerning the infestation of clonorchis sinensis in human was conducted in 4 communes of Ninh Binh and Nam Dinh provinces, from each, 100 households with their members of above 6 years old of age were enrolled randomly. Results showed a prevalence of 31.3 – 37.3% in Nam Dinh and 22.5% in Ninh Binh epidemic areas. The prevalence in male subjects was higher than in female (45.5 –47.6% versus 10.9 – 26.1%). The age group of 40-49 years old had had the highest prevalence (51.9%). There, 35.9 – 80.4% of people had consumed raw fish.
Opisthorchis ; Epidemiology ; Clonorchis sinensis

The survival ability of the larvae of Clonorchis sinensis in raw fish dish prepared by traditional culinary method in Nghia Lac commune, Nghia Hung District, Nam Dinh province and the larvae of Paragonimus heterotrimus in grilled crabs processed in Khanh Hoa commune, Luc Yen district, Yen Bai province was investigated. In this raw fish dish, 93-95% of the microcerca of Clonorchis sinensis and in this grilled crab, 23.3-65% of microcerca had been living
Clonorchis sinensis ; Fishes ; Paragonimus

Clonorchis sinensis, the most widespread liver fluke of the parasite localized in liver and biliary tract, threats at least 19 millions of people in the world. The distribution of C. sinensis over almost all China (except the West-Northern area) and Taiwan, Korea, Japan and Northern regions of Vietnam. Since the first record of C. sinensis in Vietnam last century, up to date the disease has been reported from at least 12 provinces of the country. At the certain epidemiological foci, the rate of C. sinensis infection has not decreased but, in inverse, it seems to have tendency to increase. This review follows the worldwide and local literature in the domain to provide a brief summary on the distribution and status of C. sinensis infection and in the world, particularly in Vietnam.
Clonorchis sinensis ; Epidemiology

A region of mitochondrial cytochrome oxidase 1 gene (coxl) for Clonorchis sp samples from the provinces of Thanh Hoa and Nam Dinh, and Opisthorchis sp from human in Phu Yen province, Vietnam was analyzed by PCR. Nucleotide sequence of these coxl fragments was used to compare with Opisthorchis viverrini, strain Khon Ken (Thailand) and Clonorchis sinensis sequences originated from China and Korea. The results revealed that nucleotide sequence of Opisthorchis sp from Vietnam had absolute homology to O. viverrini (Thailand) but different from C. sinensis (from China and Korea). Clonorchis sp from Vietnam was similar with Clonorchis sinensis from China and Korea. Opisthorchis sp isolated in Phu Yen is, thus, molecularly indentified as Opsithorchis viverrini and Clonochis sp in Thanh Hoa and Nam Dinh is Clonorchis sinensis. Two species are different in morphology and molecular level.
Mitochondria ; Clonorchis sinensis

No abstract available.
Clonorchiasis* ; Clonorchis sinensis

4826 stool samples collected from 5 communes were examined by Kato- Katz technique. The infection rate of clonorchis sinensis was 33,9% in average (22,5% to 37,3%). The infection rate in male was higher than in female by 4 times and highest in 40- 49 age group (51,9%). The habit of eating raw fish is very common in this area (55,6%- 80,4%). The adult worms from patients in these study sites were identified as clonorchis sinensis
Clonorchis sinensis ; epidemiology ; Fishes ; Helminths

This study was undertaken to evaluate the role of peritoneal exudate cells in the transfer of immunity against the liver fluke, Clonorchis sinensis in the inbred BALB/c mice. Ten donor mice were divided into 2 groups. One group consisted of 5 mice was infected orally with 20 metacercariae of C. sinensis, and the other group was injected intraperitoneally with 20 excysted larvae. Thirty days after immunization, the peritoneal exudate cells were obtained from the donor mice. Twenty recipient mice were divided into 4 equal groups for the purpose of primary immunization. The mice of Group I were injected intraperitoneally with 2 x 10(6) peritoneal exudate cells of the donor mice infected orally, those of Group III were injected intraperitoneally with 2 x 10(6) peritoneal exudate cells of the donor mice injected intraperitoneally. Those of Group II were injected orally with 20 metacercariae of C. sinensis. The group IV mice served as controls. Four days after the primary immunization all recipient mice were challenged orally with 20 metacercariae of C. sinensis, and then killed 30 days after the challenging infection. When the peritoneal exudate cells were injected into the recipient mice, pronounced reduction in eggs per gram of the feces was found in the mice of Group I and Group II, but no reduction in those of Group III. In the worm burdens of C. sinensis, the number of flukes found in the mice of Group II was only significantly less than those in the control group(IV). In addition the number of plaque forming cells per spleen in the mice of Group II was found larger than those in Group I. It is likely that donor peritoneal exudate cells transferred to the recipients might result in the production of relative immunity.
parasitology-helminth-trematoda ; Clonorchis sinensis ; immunology ; mouse

Ultrastructural studies on the integument of digenetic trematodes were investigated by several workers (Senft et al., 1961; Bjorkman and Thorsell, 1964; Burton, l966; Erasmus, l968; Morris and Threadgold, 1967, l968). Clonorchis sinensis is a common parasite of human beings in the Far East. But as far little has been written on ultrastructure of the integument of this parasite. This study was carried out systematically to elucidate the ultrastructure of the integument of adult C. sinensis, and the results are summarized as follows: The integument of C. sinensis was organized in two parts; the epidermis and the muscular layer. The apical surface and basal surface of the epidermis were covered with typical plasma membrane and irregularly invaginated from the surface. Plasma membrane at perisuckers was extremely folded and appeared as if the epidermis might be divided with the layer. The syncytial epidermis contained mitochondria and numerous secretory bodies, and which was connected by cytoplasmic processes to nucleated epidermal cells lying in the parenchyma. The mitochondriae, the secretory bodies, the granular endoplasmic reticulum, the Golgi bodies and the free ribosomes were present in the perinuclear cytoplasm of epidermal cells, but no organelle was observed in processes of epidermal cell except some secretory bodies. The fibrous layer consists of argentophile fiber and collagenous fiber, in which the circular muscle bundles and longitudinal muscle bundles were embedded. Bundles of muscle fiber are weIl developed at the perisucker, and mitochondriae in this portion contain many cristae as compared with any other parts of C. sinensis.
parasitology-helminth-trematoda-Clonorchis sinensis ; ultrastructure ; electronmicroscopy