1.Human Cloning, Is It a Victory of Science?.
Journal of the Korean Medical Association 2003;46(4):266-267
No abstract available.
Cloning, Organism*
2.Cloning of HEV recombinant protein expressed in Pichia pastoris
Journal of Preventive Medicine 2000;10(4):46-51
The fragment in ORF2 of HEV genome, which is coded for structural protein was transformed into Pichia pastoris GS115 strain (Mut+) after confirmation of positives clones by PCR and DNA sequencing. After induction by methanol for 4 days, shaking at 30oC, the cells were collected and disrupted. The purification of recombinant protein was checked by SDS-PAGE and the presence of HEV recombinant antigen was identified by Western Blot. High antigenicity of HEV recombinant protein was constructed
Cloning, Organism
;
Proteins
3.Human Cloning.
Journal of the Korean Medical Association 1999;42(4):369-374
No abstract available.
Cloning, Organism*
;
Humans*
4.Human Cloning: Hope or Disaster.
Journal of the Korean Medical Association 2003;46(4):264-265
No abstract available.
Cloning, Organism*
;
Disasters*
;
Hope*
5.Location and cloning of the polyhedrin gene of hyphantria cunea nuclear polyhedrosis virus.
Hyung Hoan LEE ; Mi Kyung LEE ; Il Hwan CHO ; Kwan Hee YOO
Journal of the Korean Society of Virology 1991;21(1):25-34
No abstract available.
Clone Cells*
;
Cloning, Organism*
;
Nucleopolyhedrovirus*
6.Cloning and map location of thymidine kinase(TK) gene of Korean isolate bovine herpesvirus PQ strain.
Chang Hee KWEON ; Young Jin KEE ; Byung Joon KWON ; Soo Hwan AN
Journal of the Korean Society of Virology 1993;23(2):165-169
No abstract available.
Clone Cells*
;
Cloning, Organism*
;
Thymidine*
7.Cloning and protein expression of Actinobacillus actinomycetemcomitans cytolethal distending toxin subunit CdtA.
Sun Young KO ; Dong Keun JEONG ; So Hyun RYU ; Hyung Seop KIM
The Journal of the Korean Academy of Periodontology 2007;37(Suppl):339-351
No abstract available.
Actinobacillus*
;
Aggregatibacter actinomycetemcomitans*
;
Clone Cells*
;
Cloning, Organism*
8.Cloning and Expression of Low Molecule Protein Antigens from Mycobacterium tuberculosis H37Rv.
Jae Hyun LIM ; Hwa Jung KIM ; Eun Kueong JO ; Chang Hwa SONG ; Un Ok KIM ; Jeong Kyu PARK
Journal of the Korean Society for Microbiology 2000;35(5):375-375
No Abstract Available.
Clone Cells*
;
Cloning, Organism*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
9.Cloning of the hyphantrica cunea nuclear polyhedrosis virus partial EcoRI genome DNA fragments in plasmid vectors pUC8 and pBR322.
Hyung Hoan LEE ; Jin Wook KIM ; Hee Kyung KIM ; Sung Sook PARK ; Yong Chull LEE ; Dong Chull OK
Journal of the Korean Society of Virology 1991;21(1):35-40
No abstract available.
Clone Cells*
;
Cloning, Organism*
;
DNA*
;
Genome*
;
Nucleopolyhedrovirus*
;
Plasmids*
10.Cloning of yeast Pichia pastoris coded synthesis of HBsAg DNA recombinant
Journal of Preventive Medicine 2001;11(2):40-45
The DNA fragment in HBV genome, which codes for HBsAg, was reconstructed from oligodeoxynucleotides using PCR method. After checking for correct nucleotid sequences by DNA sequencing, the DNA fragment coding for HBsAg was ligated into the plasmid vector pPICZA. Restricted enzymes EcoR I and Noti were utilized to cut DNA - plasmid and positive clones containing HBsAg-coding DNA-plasmid were selected by agarose gel electrophoresis
Cloning, Organism
;
Hepatitis B Surface Antigens
;
DNA, Recombinant
Result Analysis
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