1.The role of CDI on the antigen recognition of human CD3+CD4-CD8 T lymphocyte clone specific to M.tyberculosis.
Myung Sik CHOI ; Ju Young SEOH ; Dong Gyun LIM ; Ik Sang KIM ; Woo Hyun CHANG ; Chang Yong CHA
Journal of the Korean Society for Microbiology 1993;28(6):505-519
No abstract available.
Clone Cells*
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Humans*
;
Lymphocytes*
2.Computational Approach for Biosynthetic Engineering of Post-PKS Tailoring Enzymes.
Genomics & Informatics 2008;6(4):227-230
Compounds of polyketide origin possess a wealth of pharmacological effects, including antibacterial, antifungal, antiparasitic, anticancer and immunosuppressive activities. Many of these compounds and their semisynthetic derivatives are used today in the clinic. Most of the gene clusters encoding commercially important drugs have also been cloned and sequenced and their biosynthetic mechanisms studied in great detail. The area of biosynthetic engineering of the enzymes involved in polyketide biosynthesis has recently advanced and been transferred into the industrial arena. In this work, we introduce a computational system to provide the user with a wealth of information that can be utilized for biosynthetic engineering of enzymes involved in post-PKS tailoring steps. Post-PKS tailoring steps are necessary to add functional groups essential for the biological activity and are therefore important in polyketide biosynthesis.
Clone Cells
;
Multigene Family
3.Situation of the Korean Medical Association about Animal Clone.
Journal of the Korean Medical Association 1999;42(9):826-829
No abstract available.
Animals*
;
Clone Cells*
4.The Methods and Ethical Problems in the Human Clone.
Journal of the Korean Medical Association 1997;40(9):1146-1153
No abstract available.
Clone Cells*
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Humans*
5.Construction and characterization of band-specific genomic clones by chromosome microdissection.
Gham HUR ; Eun Young KIM ; Sun Hwa PARK ; Yong Hyuck CHUN
Korean Journal of Anatomy 1993;26(4):435-449
No abstract available.
Clone Cells*
;
Microdissection*
6.Cloning and map location of thymidine kinase(TK) gene of Korean isolate bovine herpesvirus PQ strain.
Chang Hee KWEON ; Young Jin KEE ; Byung Joon KWON ; Soo Hwan AN
Journal of the Korean Society of Virology 1993;23(2):165-169
No abstract available.
Clone Cells*
;
Cloning, Organism*
;
Thymidine*
7.Location and cloning of the polyhedrin gene of hyphantria cunea nuclear polyhedrosis virus.
Hyung Hoan LEE ; Mi Kyung LEE ; Il Hwan CHO ; Kwan Hee YOO
Journal of the Korean Society of Virology 1991;21(1):25-34
No abstract available.
Clone Cells*
;
Cloning, Organism*
;
Nucleopolyhedrovirus*
8.Molecular Marker Related to Fruitbody Color of Flammulina velutipes.
Won Sik KONG ; Chang Hyun YOU ; Young Bok YOO ; Gyu Hyun KIM ; Kwang Ho KIM
Mycobiology 2004;32(1):6-10
White and brown strains of Flammulina velutipes were inter-crossed. All F1 showed light-brown fruitbody, suggesting that a gene for the brown fruitbody was incompletely dominant against the white one. And backcross experiment showed that more than two genes were involved in color determination. To isolate a molecular marker linked to fruitbody color, a set of primers was designed from a sequence of clones derived by a bulked segregant analysis. These markers showed a specific band which co-segregated with brown fruitbody forming strains.
Clone Cells
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Flammulina*
;
Genes, vif
9.Generation and Characterization of Monoclonal Antibodies against Human Interferon-lambda1.
Seung Ho HONG ; Jung Sik KIM ; Sun PARK
Immune Network 2008;8(1):7-12
BACKGROUND: Members belonging to the interferon-lambda (IFN-lambda) family exert protective action against viral infection; however, the mechanisms of their action have remained elusive. To study IFN-lambda biology, such as endocytosis of IFN-lambda, we produced monoclonal antibodies (Abs) against human IFN-lambda and examined their usefulness. METHODS: We purified recombinant human IFN-lambda1 expressed in Escherichia coli by using affinity columns. Then, we generated hybridoma cells by fusing myeloma cells with splenocytes from IFN-lambda1- immunized mice. For evaluating the neutralizing activity of the monoclonal Abs against IFN-lambda1, we performed RT-PCR for the MxA transcript. In order to study the binding activity of IFN-lambda and the monoclonal Ab complex on HepG2 cells, we labeled the monoclonal Ab with rhodamine and determined the fluorescence intensity. RESULTS: Four hybridoma clones secreting Abs specific to IFN-lambda1 were generated and designated as HL1, HL2, HL3, and HL4. All the Abs reacted with IFN-lambda1 in the denatured form as well as in the native form. Abs produced by HL1, HL3, and HL4 did not neutralize the induction of the MxA gene by IFN-lambda1. We also demonstrated the binding of the HL1 monoclonal anbitody and IFN-lambda complex on HepG2 cells. CONCLUSION: Monoclonal Abs against IFN-lambda1 were produced. These Abs can be used to study the cellular binding and internalization of IFN-lambda.
Animals
;
Antibodies, Monoclonal
;
Biology
;
Clone Cells
;
Endocytosis
;
Escherichia coli
;
Fluorescence
;
Hep G2 Cells
;
Humans
;
Hybridomas
;
Mice
;
Rhodamines
10.Generation and Characterization of Monoclonal Antibodies against Human Interferon-lambda1.
Seung Ho HONG ; Jung Sik KIM ; Sun PARK
Immune Network 2008;8(1):7-12
BACKGROUND: Members belonging to the interferon-lambda (IFN-lambda) family exert protective action against viral infection; however, the mechanisms of their action have remained elusive. To study IFN-lambda biology, such as endocytosis of IFN-lambda, we produced monoclonal antibodies (Abs) against human IFN-lambda and examined their usefulness. METHODS: We purified recombinant human IFN-lambda1 expressed in Escherichia coli by using affinity columns. Then, we generated hybridoma cells by fusing myeloma cells with splenocytes from IFN-lambda1- immunized mice. For evaluating the neutralizing activity of the monoclonal Abs against IFN-lambda1, we performed RT-PCR for the MxA transcript. In order to study the binding activity of IFN-lambda and the monoclonal Ab complex on HepG2 cells, we labeled the monoclonal Ab with rhodamine and determined the fluorescence intensity. RESULTS: Four hybridoma clones secreting Abs specific to IFN-lambda1 were generated and designated as HL1, HL2, HL3, and HL4. All the Abs reacted with IFN-lambda1 in the denatured form as well as in the native form. Abs produced by HL1, HL3, and HL4 did not neutralize the induction of the MxA gene by IFN-lambda1. We also demonstrated the binding of the HL1 monoclonal anbitody and IFN-lambda complex on HepG2 cells. CONCLUSION: Monoclonal Abs against IFN-lambda1 were produced. These Abs can be used to study the cellular binding and internalization of IFN-lambda.
Animals
;
Antibodies, Monoclonal
;
Biology
;
Clone Cells
;
Endocytosis
;
Escherichia coli
;
Fluorescence
;
Hep G2 Cells
;
Humans
;
Hybridomas
;
Mice
;
Rhodamines