Perianal necrotizing fasciitis is a serious soft tissue infectious disease of perianal and perineal regions, where a synergistic action of multiple bacteria (including aerobic bacteria and anaerobic bacteria) results in suppurative necrosis of the skin and soft tissue. The disease progress may rapidly cause systemic sepsis through blood circulation, often with complicating shock and MODS, or death. Any delay in diagnosis and treatment of early acute infections may lead to higher mortality because of lack of standardized treatment. The Clinical Guidelines Committee aims to formulate expert consensus on the treatment of perianal necrotizing fasciitis in terms of etiology and pathology, clinical manifestations, laboratory and imaging study, preoperative preparation, surgical treatment (the application of antibiotics, the timing and key points of debridement, assistant therapy), postoperative wound care, nutritional support, surgical reconstruction and rehabilitation. This consensus is a reference for clinicians based on patient conditions.

Objective Alarge-scale prospective multicenter randomized controlled tial was conducted to evaluate the efficacy and safety of Yinzhihuang oral solution for the treatment of neonatal indirect hyperbilirubinemia in term newborn infants. Yinzhihuang oral solution is a herbal extract with the main components of Herba Artemisiae Scopariae, Scutellaria, Lonicera Japonica and Gardenia jasminoides. Methods A total of 16 hospitals participated in this study. From March to September 2010, the term infants whose bilirubin level ≥ 40 th percentile for age in hours were enrolled, except for those who received exchange transfusion or had signs of bilirubin encephalopathy. All the 1177 cases were divided randomly into three groups: phototherapy group (409 cases ), phenobarbital combined with phototherapy group (373 cases) and Yinzhihuang oral liquid combined with phototherapy group (395 cases). Phenobarbital and Yinzhihuang oral liquid was started once the infants participated the study, and persisted for 5 days. Phototherapy was added as soon as the bilirubin level reached the lowest threshold ( the threshold for infants at higher risk). The demographic data of infants in each group were recorded, the serum bilirubin level before treatment, after treatment for 72 hours and after the treatment completion were checked. The reduction rate of serum bilirubin and the phototherapy rate in different groups were compared. The adverse events were assessed as well. Results Of the total of 1177 cases, 707 (60. 1％ ) were male, 1119 cases (95. 1％ ) were of Han ethnicity. The average total bilirubin level before treatment was ( 282. 0 ± 70. 9) μmol/L and the highest level was 626 μmol/L The severe hyperbilirubinemia (total bilirubin level at 342 μmoL/L to 427 μmol/L) accounted for 15.8％ (186 cases), and the extremely severe hyperbilirubinemia (total bilirubin ＞427 μmol / L) accounted for 2. 5％ (30 cases). After treatment for 72 hours, the reduction of bilirubin was not significantly different among three groups ( F =2. 89, P =0. 056). After completion of treatment, the reduction rate of bilirubin in Yinzhihuang group was higher than that of the other two groups (F =5.55, P =0. 004). The rate of infants who did not receive phototherapy in Yinzhihuang group was higher than that in phenobarbital group (x2 =47. 38, P=0. 000). In Yinzhihuang group, more infants had bowel movements more than five times a day. The incidence of rashes was higher than that in phenobarbital group (P =0. 019), but no significant difference was found as compared with that in phototherapy group (P =0. 339). Conclusions About 18％ of the term infants who were admitted for jaundice had severe or extremely severe hyperbilirubinemia. Yinzhihuang oral solution combined with phototherapy is effective in bilirubin reduction. Early treatment with Yinzhihuang oral solution may inhibit further increase in bilirubin levels, reduce the phototherapy requirement.

Perianal necrotizing fasciitis is a serious soft tissue infectious disease of perianal and perineal regions, where a synergistic action of multiple bacteria (including aerobic bacteria and anaerobic bacteria) results in suppurative necrosis of the skin and soft tissue. The disease progress may rapidly cause systemic sepsis through blood circulation, often with complicating shock and MODS, or death. Any delay in diagnosis and treatment of early acute infections may lead to higher mortality because of lack of standardized treatment. The Clinical Guidelines Committee aims to formulate expert consensus on the treatment of perianal necrotizing fasciitis in terms of etiology and pathology, clinical manifestations, laboratory and imaging study, preoperative preparation, surgical treatment (the application of antibiotics, the timing and key points of debridement, assistant therapy), postoperative wound care, nutritional support, surgical reconstruction and rehabilitation. This consensus is a reference for clinicians based on patient conditions.

To investigate the relationship of biofilm-forming ability of (PA) with swimming motility, twitching motility and virulence gene distribution. A total of 192 clinical isolates of PA were collected consecutively. Microtiter plate method was used to evaluate the ability to form biofilm. The swimming and twitching motilities were detected by plate method. Polymerase chain reaction (PCR) was used to detect virulence genes. Of the 192 PA clinical isolates, 186 (96.9%) showed biofilm-forming ability. Among them, 36 isolates showed weak biofilm-forming ability, 84 exhibited moderate biofilm-forming ability and 66 showed strong biofilm-forming ability. The diameters of the swimming ring for PA with none biofilm-forming ability, weak biofilm-forming ability, moderate biofilm-forming ability, strong biofilm-forming ability were (9.12±6.76), (18.42±7.51), (19.10±4.77) and respectively. The diameters of the twitching ring for PA in above groups were (8.38±1.50), (17.21±7.42), (18.49±5.62) and respectively. The swimming motility and twitching motility of none biofilm-forming ability group were weaker than biofilm-forming ability groups (all <0.05). Among 192 PA strains, 163 were positive (84.9%), 40 were positive (20.8%), 183 were positive (95.3%), and 189 were positive (98.4%). The positive rate of PA virulence gene , and were different in strains with different biofilm-forming abilities (<0.05). The rate of in the strong biofilm-forming ability group was lower than that in the moderate biofilm-forming ability group (=9.293, <0.01) and the weak biofilm-forming ability group (=9.997, <0.01). The rate of in the strong biofilm-forming ability group was higher than that in the weak biofilm-forming ability group (=10.803, <0.01). Most clinical isolates of PA can form biofilm. Swimming and twitching motilities are related to the formation of biofilm, but not significantly related to strength of biofilm-forming ability. The virulence genes of type Ⅲ secretion system for PA may be related to the biofilm-forming ability.
Biofilms ; Humans ; Swimming ; Virulence/genetics*

Objective: To investigate the effects of TFPI-2 (tissue factor pathway inhibitor 2) on the growth and angiogenesis of subcutaneous tumor xenografts in nude mice established from human liver cancer cells. Methods: The Hep3B cells stably expressing TFPI-2 (Hep3B-TFPI-2 group) and the Hep3B cells transfected with empty vector PCDNA3.1 (Hep3B-V group) or without transfection (Hep3B-P group) were subcutaneously transplanted into nude mice respectively to generate subcutaneous tumor xenografts. The volume of tumor xenograft was measured every three days, and the growth curve of tumor xenograft was drawn when the subcutaneous tumor xenograft was visible. The nude mice were killed three weeks after transplant, the volume of tumor xenograft was measured, and the total RNAs and proteins in tumor xenografts were extracted. The mRNA and protein expressions of TFPI-2 and VEGF (vascular endothelial growth factor) in tumor xenografts were analyzed by RFQ-PCR (real-time fluorescence quantitative PCR) and Western blotting, respectively. The expression of TFPI-2 protein and the MVD (microvessel density) in tumor xenografts were observed by immunohistochemistry. Results: The eventual tumor volume of tumor xenografts in Hep3B-TFPI-2 group was apparently smaller than those in Hep3B-V group and Hep3B-P group (both P < 0.05). The expression of mRNA and abundance of protein of TFPI-2 in Hep3B-TFPI-2 group were significantly higher than those in the other two groups (P < 0.05); while the expression of mRNA and abundance of protein of VEGF in Hep3B-TFPI-2 group were apparently lower than those in the other two groups. Compared with Hep3B-V group and Hep3B-P group, the inhibitory rates of VEGF protein expression in Hep3B-TFPI-2 group were 19.8% and 23.5%, respectively (P < 0.05). The MVD in Hep3B-TFPI-2 group was apparently lower than those in the other two groups (P < 0.05). Conclusion: TFPI-2 can significantly inhibit the growth and angiogenesis of subcutaneous tumor xenografts in nude mice established from hepatocarcinoma Hep3B cells. Copyright © 2013 by TUMOR.

Objective: To investigate the effects of fusion peptide cytoplasmic transduction peptide-oligomerization domain 1-hemagglutinin (CTP-OD1-HA) and CTP-OD2-HA on subcutaneous xenograft of leukemia K562 cells in nude mice. Methods: K562 cells were pre-treated with CTP-OD-HA (as a positive control), CTP-HA (as a negative control), PBS (as a blank control), CTP-OD1-HA and CTP-OD2-HA, respectively, then the cells were injected into nude mice subcutaneously. The growth of the subcutaneous xenograft was observed. The apoptosis of the tumor cells in subcutaneous xenograft was detected by TUNEL method. The expression levels of Bax and Bcl-2 proteins in subcutaneous xenograft were examined by immunohistochemistry. Results: The volumes of the subcutaneous xenografts in CTP-OD1-HA group and CTP-OD2-HA group were smaller than that in CTP-HA group (P < 0.05), with the change of cavitation apoptosis. The apoptotic change of tumor cells was significant in CTP-OD1-HA group and CTP-OD2-HA group by TUNEL method (P < 0.05). The expression level of apoptosis-associated protein Bax was higher in CTP-OD1-HA group and CTP-OD2-HA group than that in CTP-HA group (P < 0.05), whereas the expression level of Bcl-2 was lower (P < 0.05). Conclusion: CTP-OD1-HA and CTP-OD2-HA fusion peptide can inhibit the tumorigenicity of leukemia K562 cells in nude mice and promote the apoptosis. Copyright © 2013 by TUMOR.

Objective: To establish multiple human hepatocellular carcinoma (HCC) multidrug-resistant cell lines induced by chemotherapeutic drugs, and to investigate the effects of multidrug-resistance on cell proliferation, apoptosis and invasion activity and the relationship between the multidrug-resistance and the expression of mitogen-activated protein kinase (MAPK) pathway. Methods: Three different human HCC cell strains HepG2, SMMC-7721 and BEL-7402 were established by using pulse treatment with high concentration of adriamycin (ADM) or treatment with ADM of low concentration gradually increased. The difference between the resistant cells and the parental cells was evaluated. The drug sensitivity was tested by ATP bioluminescence to calculate the resistance index (RI). The expressions of multidrug resistance-related genes including P-glycoprotein (P -gp ), multidrug resistance-associated protein 1 (MRP 1), lung cancer resistance protein 1 (LRP 1), breast cancer resistance protein (BCRP ), glutathione-S - transferase-π (GST -π), topoisomerase IIβ (ToPo IIβ) and protein kinase C (PKC ) and their proteins were detected by RT-PCR and immunohistochemistry (IHC), respectively. The expressions of cell proliferative activity-related Ki-67 and proliferating cell nuclear antigen (PCNA) were detected by IHC. The cell cycle and apoptosis rate were detected by flow cytometry (FCM), and the change of cell invasion ability was detected by Transwell assay. The expressions of ERK 1, ERK 2, ERK 5, JNK 1, JNK 2 and p 38a genes and their proteins in MAPK pathway were detected by RT-PCR and Western boltting, respectively. Results: Six ADMresistant cell strains were established. Compared to parental cells, the RIs of all resistant cells were over 5 with increased resistance to multiple chemotherapeutic drugs. The expressions of MDR genes and their proteins were increased 2-10 times, especially for the cells induced by pulse treatment with high concentration of ADM. The expressions of Ki-67 and PCNA proteins were increased over 20 times in the resistant cells. The cell cycles were arrested at phase S, and the invasive activities in vitro were increased 1.3-2.5 times. The apoptosis rates were decreased by over 60% after ADM intervention. The expressions of MAPK pathway-related genes and proteins were increased in varying degrees, especially for ERK1 and ERK2. Conclusion: ADM can induce the multidrug-resistance in multiple HCC cell strains and increase the cell proliferation activity, alter the cell cycle distribution, strengthen the anti-apoptosis effect and enhance the invasive ability of the HCC cells. The multidrug-resistance maybe associated with the upregulation of MAPK pathway. Copyright © 2012 by TUMOR.

Objective: To investigate the growth inhibition effects of pemetrexed and oxaliplatin on human gastric cancer cell line BGC-823 in vitro, and to explore the possible mechanism. Methods: The BGC-823 cells were treated with different concentrations of pemetrexed or oxaliplatin alone or in combination. The growth inhibition rate of BGC-823 cells was detected by MTT method, and the change of cell cycle distribution was analyzed by flow cytometry (FCM). The expressions levels of proliferating cell nuclear antigen (PCNA) and cell cycle D1 (cyclin D1) proteins in BGC-823 cells were examined by Western blotting. Results: The growth of BGC-823 cells was inhibited by pemetrexed or oxaliplatin in a dose- and time-dependent manner (P £?0.05). The percentage of cells in G1 phase was increased while which in S phase was decreased by the treatment of pemetrexed or oxaliplatin. The expression levels of PCNA and cyclin D1 proteins were significantly decreased, and the combination regimen of pemetrexed and oxaliplatin exerted a synergistic effect. The growth inhibition rate of BGC-823 cells treated with pemetrexed (80 μg/mL) for 24 h followed by oxaliplatin (40 μg/mL) for 24 h was significantly decreased as compared with those of the cells treated with pemetrexed (80 μg/mL) combined with oxaliplatin (40 μg/mL) at the same time or treated with oxaliplatin (40 μg/mL) for 24 h followed by pemetrexed (80 μg/mL) for 24 h (P<0.05). Conclusion: Pemetrexed and oxaliplatin can both inhibit the proliferation of gastric cancer BGC-823 cells and arrest the cells at G1 phase. The sequential regimen of pemetrexed followed by oxaliplatin may have the strongest growth-inhibitory effect on gastric cancer cells among different doublet regimens. Copyright© 2011 by TUMOR.