1.H3K27me3 Signal in the Cis Regulatory Elements Reveals the Differentiation Potential of Progenitors During Drosophila Neuroglial Development.
Xiaolong CHEN ; Youqiong YE ; Liang GU ; Jin SUN ; Yanhua DU ; Wen-Ju LIU ; Wei LI ; Xiaobai ZHANG ; Cizhong JIANG
Genomics, Proteomics & Bioinformatics 2019;17(3):297-304
Drosophila neural development undergoes extensive chromatin remodeling and precise epigenetic regulation. However, the roles of chromatin remodeling in establishment and maintenance of cell identity during cell fate transition remain enigmatic. Here, we compared the changes in gene expression, as well as the dynamics of nucleosome positioning and key histone modifications between the four major neural cell types during Drosophila neural development. We find that the neural progenitors can be separated from the terminally differentiated cells based on their gene expression profiles, whereas nucleosome distribution in the flanking regions of transcription start sites fails to identify the relationships between the progenitors and the differentiated cells. H3K27me3 signal in promoters and enhancers can not only distinguish the progenitors from the differentiated cells but also identify the differentiation path of the neural stem cells (NSCs) to the intermediate progenitor cells to the glial cells. In contrast, H3K9ac signal fails to identify the differentiation path, although it activates distinct sets of genes with neuron-specific and glia-related functions during the differentiation of the NSCs into neurons and glia, respectively. Together, our study provides novel insights into the crucial roles of chromatin remodeling in determining cell type during Drosophila neural development.
2.BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding.
Mingzhu WANG ; Kun ZHAO ; Meng LIU ; Mengting WANG ; Zhibin QIAO ; Shanru YI ; Yonghua JIANG ; Xiaochen KOU ; Yanhong ZHAO ; Jiqing YIN ; Tianming LI ; Hong WANG ; Cizhong JIANG ; Shaorong GAO ; Jiayu CHEN
Protein & Cell 2022;13(8):580-601
Chemically defined medium is widely used for culturing mouse embryonic stem cells (mESCs), in which N2B27 works as a substitution for serum, and GSK3β and MEK inhibitors (2i) help to promote ground-state pluripotency. However, recent studies suggested that MEKi might cause irreversible defects that compromise the developmental potential of mESCs. Here, we demonstrated the deficient bone morphogenetic protein (BMP) signal in the chemically defined condition is one of the main causes for the impaired pluripotency. Mechanistically, activating the BMP signal pathway by BMP4 could safeguard the chromosomal integrity and proliferation capacity of mESCs through regulating downstream targets Ube2s and Chmp4b. More importantly, BMP4 promotes a distinct in vivo developmental potential and a long-term pluripotency preservation. Besides, the pluripotent improvements driven by BMP4 are superior to those by attenuating MEK suppression. Taken together, our study shows appropriate activation of BMP signal is essential for regulating functional pluripotency and reveals that BMP4 should be applied in the serum-free culture system.
Animals
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Bone Morphogenetic Protein 4/metabolism*
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Cell Differentiation
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Chromosomal Instability
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Endosomal Sorting Complexes Required for Transport
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Mice
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Mitogen-Activated Protein Kinase Kinases/metabolism*
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Mouse Embryonic Stem Cells/cytology*
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Pluripotent Stem Cells/cytology*
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Signal Transduction
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Ubiquitin-Conjugating Enzymes