1.Neurotrophins up-express in peripheral blood of allergic rhinitis patients and related to Th2 hypothesis.
Hao WANG ; Renhui CHEN ; Yanqing ZHONG ; Yiqun GENG ; Ciyun XU ; Weibiao KANG ; Yulong LIN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(14):1024-1027
OBJECTIVE:
To detect the expression of NGF, BDNF, NT-3 mRNA in the peripheral blood of patients with allergic rhinitis (AR). And to analyze the correlation between NGF, BDNF, NT-3 mRNA expression and the epidsode of rhinitis through Th-2 Hypothesis.
METHOD:
This study was a group controlled trial. The expression of NGF, BDNF and NT-3 mRNA were tested by real-time quantitative RT-PCR and the concentrations of IL-4, IL-6, IL-10 and INF-alpha were tested by ELISA.
RESULT:
The expression of NGF, BDNF and NT-3 mRNA in AR patients were 2.44, 4.46 and 1.78 times the amount of those in the healthy adults, respectively. The increased expression of NT-3 correlated positively with the scores of visual analog scale of AR. The concentrations of IL-4, IL-6 and IL-10, which were 2198 +/- 472 pg/mL, 9407 +/- 703 pg/mL and 3917 +/- 323 pg/mL respectively, were higher than those in the healthy adults. The concentration of INF-alpha was 2198 +/- 472 pg/mL and less than the healthy adults. The increased expressions of NGF, NT-3 were positively related to the increase of IL-4, IL-6 and IL-10.
CONCLUSION
The expressions of NGF, BDNF and NT-3 mRNA in AR patients are higher than those in the healthy adults. NGF, BDNF and NT-3 may contribute to the pathogenesis of AR. Moreover, NGF and NT-3 may induce the episode of rhinitis through Th-2 Hypothesis.
Adolescent
;
Adult
;
Brain-Derived Neurotrophic Factor
;
blood
;
Case-Control Studies
;
Child
;
Female
;
Humans
;
Interleukin-10
;
blood
;
Interleukin-4
;
blood
;
Interleukin-6
;
blood
;
Male
;
Nerve Growth Factor
;
blood
;
Nerve Growth Factors
;
blood
;
genetics
;
Neurotrophin 3
;
blood
;
RNA, Messenger
;
genetics
;
Rhinitis, Allergic
;
blood
;
immunology
;
Th1-Th2 Balance
;
Young Adult