BACKGROUND: Pectin is a heteropolysaccharide used in pharmaceutical formulations as a binding agent. Importation of pectin costs billions of Philippine pesos, but the local laboratory-scale production of this excipient from fruit peel wastes is estimated to be cheaper by 80%.

OBJECTIVE: To address economic and environment concerns associated with pectin production, this study aimed to optimize the isolation and purification of pharmaceutical grade pectin from pomelo (Citrus maxima Merr.) fruit peel as basis for commercial-scale production.

METHODS: Pectin was extracted from pomelo using different solvents: 6.2% w/w citric acid, 1N acetic acid, 3N hydrochloric acid, 3N nitric acid, and 3N sulfuric acid. Temperatures for extracting pectin were explored at 40°C, 60°C, and 90°C. Obtained pectin samples were characterized based on the following parameters: equivalent weight (EW), methoxyl content (MC), ash content (AC), anhydrouronic acid content (AUA), and degree of esterification (DE).

RESULTS: Highest pectin yield (9.25%) was obtained using 3N nitric acid and 3N sulfuric acid at 90°C.Based from the pharmacopeial standards (MC ? 6.7, AUA ? 74.0), all the samples did not pass the parameters, except the pectin extracted using 3N sulfuric acid at 90°C (MC = 6.76, AUA = 74.61).

CONCLUSION: Among the different solvents used for extraction, 3N sulfuric acid produced the highest percent yield of pharmaceutical grade pectin from pomelo fruit peel. Its optimum temperature for extraction was at 90°C. The sample passed the USP standards of MC values not less than 6.76 and AUA values not less than 74. Under the following conditions, pomelo fruit peel have the potential for commercial-scale production of pharmaceutical grade pectin.

No abstract available.
Asthma* ; Citrus* ; Mites*

Lecanora muralis was found on the rock along coastal line during the field trip in Jeju island in 2006. Thallus crustose, placodioid, closely adnate, forming orbicular patches; upper surface grayish green, glossy; central lobes areolate, marginal parts plane, edges thin pruinose; lower surface ecorticate; apothecia sessile, lecanorine type, exciple dense and intact when young, and disc plane, but when mature, exciple laciniate, disc protrudent, yellowish brown to orange, 0.5~1.5 mm in diameter; ascospores ellipsoid, simple, colorless, 12.5~15.0 x 5.0~7.5 microm. Usnic acid and zeorin contained in thallus. This is the first record of this species in South Korea.
Citrus sinensis ; Korea*

There are various factors affecting the fracture of NiTi rotary files. This study was performed to evaluate the effect of cross sectional area, pecking motion and pecking distance on the cyclic fatigue fracture of different NiTi files. Five different NiTi files-Profile(R)(Maillefer, Ballaigue, Switzerland), ProTaper(TM) (Maillefer, Ballaigue, Switzerland), K3(R) (SybronEndo, Orange, CA), Hero 642(R) (Micro-mega, Besancon, France), Hero Shaper(R)(Micro-mega, Besancon, France)-were used. Each file was embedded in temporary resin, sectioned horizontally and observed with scanning electron microscope. The ratio of cross-sectional area to the circumscribed circle was calculated. Special device was fabricated to simulate the cyclic fatigue fracture of NiTi file in the curved canal,. On this device, NiTi files were rotated (300rpm) with different pecking distances (3 mm or 6 mm and with different motions (static motion or dynamic pecking motion). Time until fracture occurs was measured. The results demonstrated that cross-sectional area didn't have any effect on the time of file fracture. Among the files, Profile(R) took the longest time to be fractured. Between the pecking motions, dynamic motion took the longer time to be fractured than static motion. There was no significant difference between the pecking distances with dynamic motion, however with static motion, the longer time was taken at 3mm distance. In this study, we could suggest that dynamic pecking motion would lengthen the time for NiTi file to be fractured from cyclic fatigue.
Citrus sinensis ; Fatigue* ; Fractures, Stress

Hesperidin, which is the most abundant flavonoid of Citrus unshiu (Rutaceae), has been reported to possess diverse activities and widely used as functional foods and cosmetics. For the development of functional products, extraction procedure is indispensable. Extraction conditions affect the composition of extract as well as its biological activity. Therefore, we tried to optimize extraction conditions such as extraction solvent, extraction time and extraction temperature for maximum yield of hesperidin using response surface methodology with threelevel-three-factor Box-Behnken design (BBD). Regression analysis showed a good fit of the experimental data and the optimal condition was obtained as ethanol concentration, 59.0%; temperature 71.5degrees C and extraction time, 12.4 h. The hesperidin yield under the optimal condition was found to be 287.8 microg per 5 mg extract, which was well matched with the predicted value of 290.5 microg. These results provides optimized extraction condition for hesperidin and might be useful for the development of hesperidin as functional products like health supplements, cosmetics and medicinal products.
Citrus* ; Ethanol ; Functional Food ; Hesperidin*

No abstract available.
Citrus* ; Hypersensitivity* ; Mites* ; Spiders* ; Tetranychidae*

Twenty-three isolates of Fusarium spp. were obtained from decayed citrus fruits in the fields and storages in 1998-1999. Of them, six and five isolates belonged to F. proliferatum and F. moniliforme, respectively, which were the most common. F. solani and F. sambucinum had each two isolates, F. equiseti had one isolate and seven isolates were unidentified. They produced symptoms of two types in pathogenicity test: those with leathery, beige to light or dark brown, and sunken lesions without surface mycelium (type-1) and those with lesions covered with white, beige or pink surface mycelium (type-2). Four of six isolates identified to F. proliferatum and two unidentified isolates produced type-1 lesions, and all isolates identified to F. moniliforme, F. solani, F. sambucinum, F. equiseti and five unidentified isolates produced type-2 lesions.
Citrus* ; Fruit* ; Fusarium* ; Mycelium ; Virulence

The ripe dried fruit of citron(Citrus medica) is one of the important sources of Chinese herb Citri Fructus. At the same time, it is also grown for edible and ornamental uses. There are many species and abundant genetic variation. To clarify the intraspecific variation and resource distribution of citron, this study investigated the variation in 11 citron fruits, basically covering the main species in China, including Xiaoguo citron(C. medica var. ethrog), Goucheng(C. medica var. yunnanensis), Muli citron(C.medica var. muliensis), Dehong citron(C.medica×Citrus spp.), Fuzhou citron(C.medica×C.grandis?), Mawu(C.medica×C.grandis?), Cangyuan citron, Binchuan citron, Sweet citron, Big citron, and Small citron. The natural communities of citron were proved to be mainly distributed in the southwestern and western Yunnan and southeastern Tibet of China, with Yunnan, Sichuan, Guangxi, Chongqing, Hubei, and Zhejiang identified as the main production areas. Citron has also been widely grown in India, the Mediterranean region, and the Caribbean coast countries. The field investigation revealed the large-scale intraspecific variation of citron fruits. Most of the fruits are oval-like or sphere-like in shape. The fruits are green when raw and yellow when ripe, with oil cell dots on the skin, stripe-likes running from top to bottom, and bulge at the top. Usually, in the smaller citron fruits, the pulp and juice vesicles are better developed and the central columella is tighter. By contrast, the juice vesicles and central columella in larger fruits became more vacant, with carpels visible, and the apex segregation and development of the carpels is one of the reasons for variation. These variations should be given top priority in the future variety selection and breeding, and the quality differences of different citron species and their mechanisms should be further studied. In particular, variety selection and classification management according to their medicinal or edible purposes will provide scientific and technological supports for the orderly, safe, and effective production of citron products consumed as food and medicine.
China ; Citrus ; Fruit ; Taste ; Tibet

STATEMENT OF PROBLEM: It could be hypothesised that attachments, which provide more retention against vertical and horizontal dislodgement, will be associated with more favorable parameters of oral function. PURPOSE: This study was to provide data of initial retentive force and retention loss of different bar attachment systems recommended for use with maxillary implant overdentures. MATERIAL AND METHOD: 4 implants were placed in the anterior region of edentulous maxilla, five different systems of bar attachment were fabricated as follows: cantilevered Hader bar using clips (Type 1), Hader bar using clips without cantilever (Type 2), Hader bar using clip and ERA attachment orange male (Type 3), Hader bar using clip and ERA attachment white male (Type 4), and Bar using magnets (Type 5). Each samples were placed in the universal testing machine for determination of retentive forces(at initial and after every 200 cycles up to 1,000 cycles). Results and Conclusion 1. Attachment type 1 showed the biggest initial retentive force followed by type 3, type 2, type 4, and lastly type 5( p<0.001). 2. After 1,000 cycles of repeated removals of attachments, significant loss of retentive forces was taken place except for attachment type 5. 3. After 1,000 cycles of repeated removals, the loss of retentive force between type 1 and type 2, which used Hader bar and clip attachments, was greater in type 1 that had wider clip formation. And between type 3 and type 4, which used ERA attachments, the loss of retentive force was greater in type 4 that had white male attached (p<0.001). 4. After 1,000 cycles of repeated removals, attachment type 3 showed the biggest retentive force followed by type 2, type 4, type 1 and lastly type. 5. There was no significant difference between attachment type 3 and 4, and type 4 and 1(p<0.001).
Citrus sinensis ; Denture, Overlay* ; Humans ; Male ; Maxilla

Thin-layer (uni-dimensional and bi-dimensional) chrornatography studies were performed on 60 brownish stockings from 12 companies in Korea to search for Disperse Yellow 3 and Disperse Orange 3, which are known as common sensitizers in textiles. The results were as follows : 1) Mixture of various-colored dyes were being used to reveal the brownish hue in the stockings. 2) TLC demonstrated the presence of Disperse Yellow 3 in 53 stocking. 3) TLC demonstrated the presence of Disperse Orange 3 in 3 stockings, Although lack or more sophisticsted tests such as spectroseopic, infra red or nuclear spectra identification. These results suggest a strong clue to the presence of Disperse Yellow 3 and Disperse Orange 3 in the commercial stocking now being used in Korea.
Chromatography* ; Citrus sinensis* ; Coloring Agents ; Korea ; Textiles