During a survey of fungal species in South Korea, a species of Volutella ciliata was isolated and described based on the analysis of the internal transcribed spacer region of its rDNA and its morphological characteristics. This is the first record of Volutella ciliata isolated from crop field soil in Korea.
Ciliophora* ; DNA, Ribosomal ; Korea ; Sensilla ; Soil*

As a new culture mediwn for the Ciliate, Balantidium coili, isolated from a plantar ulcer of a leprosy patient, Sabouraud glucose agar medium(SGA) had several advantages: 1. it was easily made, and 2) the culture grew faster and longer than any previriusly discovered media. Of the contents of SGA, dextrose and peptene are essential nutrient, for the growth of Balantidium coli. When we cultivated Balantidiuum coli. we founund that it produced a nuueoid eubstance and a sweet smilling arcma, forming a colony like tacteria The new straining metlnd for Balantiduin coli. were 3% iodine and carbol-fuchsin stain which atained trophazaite, and cysts more sirnply and distinctly, retaining their original form.
Agar ; Balantidium* ; Ciliophora* ; Foot Ulcer* ; Glucose ; Humans ; Iodine ; Leprosy*

Ciliates are one of the oldest living eukaryotic unicellular organisms, widely distributed in the waters around the world. As a typical marine oligotrich ciliate, Strombidium sulcatum plays an important role in marine food webs and energy flow. Here we report the first deep sequencing and analyses of RNA-Seq data from Strombidium sulcatum. We generated 42,640 unigenes with an N50 of 1,451 bp after de novo assembly and removing rRNA, mitochondrial and bacteria contaminants. We employed SPOCS to detect orthologs from S. sulcatum and 17 other ciliates, and then carried out the phylogenomic reconstruction using 127 single copy orthologs. In phylogenomic analyses, concatenated trees have similar topological structures with concordance tree on the class level. Together with phylogenetic networks analysis, it aroused more doubts about the placement of Protocruzia, Mesodinium and Myrionecta. While epiplasmic proteins are known to be related to morphological characteristics, we found the potential relationship between gene expression of epiplasmic proteins and morphological characteristics. This work supports the use of high throughput approaches for phylogenomic analysis as well as correlation analysis between expression level of target genes and morphological characteristics.
Ciliophora ; genetics ; metabolism ; Phylogeny ; RNA, Protozoan ; genetics ; metabolism ; Transcriptome ; physiology