1.Alterations in cardiac structure and function in a modified rat model of myocardial hypertrophy.
Wen-Jun, DAI ; Qi, DONG ; Min-Sheng, CHEN ; Lu-Ning, ZHAO ; Ai-Lan, CHEN ; Zhen-Ci, LI ; Shi-Ming, LIU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(5):626-33
This study was aimed to establish a stable animal model of left ventricular hypertrophy (LVH) to provide theoretical and experimental basis for understanding the development of LVH. The abdominal aorta of male Wistar rats (80-100 g) was constricted to a diameter of 0.55 mm between the branches of the celiac and anterior mesenteric arteries. Echocardiography using a linear phased array probe was performed as well as pathological examination and plasma B-type natriuretic peptide (BNP) measurement at 3, 4 and 6 weeks after abdominal aortic constriction (AAC). The results showed that the acute mortality rate (within 24 h) of this modified rat model was 8%. Animals who underwent AAC demonstrated significantly increased interventricular septal (IVS), LV posterior wall (LVPWd), LV mass index (LVMI), cross-sectional area (CSA) of myocytes, and perivascular fibrosis; the ejection fraction (EF), fractional shortening (FS), and cardiac output (CO) were consistently lower at each time point after AAC. Notably, differences in these parameters between AAC group and sham group were significant by 3 weeks and reached peaks at 4th week. Following AAC, the plasma BNP was gradually elevated compared with the sham group at 3rd and 6th week. It was concluded that this modified AAC model can develop LVH, both stably and safely, by week four post-surgery; echocardiography is able to assess changes in chamber dimensions and systolic properties accurately in rats with LVH.
2.Characteristics of tidal breathing pulmonary function in children with tracheobronchomalacia.
Lan LI ; Qaing CHEN ; Fan ZHANG ; Shuang-Gui ZHU ; Ci-Lang HU ; Ai-Min WU
Chinese Journal of Contemporary Pediatrics 2017;19(12):1248-1251
OBJECTIVETo investigate the characteristics of tidal breathing pulmonary function in children with tracheobronchomalacia (TBM).
METHODSIn this study, 30 children who were diagnosed with TBM using electronic bronchoscopy were enrolled in the observation group; 30 healthy children were recruited in the normal control group. For individuals in each group, the assessment of tidal breath pulmonary function was performed at diagnosis and 3, 6, 9, and 12 months after diagnosis.
RESULTSThere were no significant differences in tidal volume, inspiratory time, expiratory time, and inspiratory to expiratory ratio between the two groups (P>0.05). Compared with the control group, the observation group had a significantly higher respiratory rate and significantly lower ratio of time to peak tidal expiratory flow to total expiratory time (TPTEF/TE) and ratio of volume to peak tidal expiratory flow to total expiratory volume (VPTEF/VE). There was a time-dependent increase in TPTEF/TE and VPTEF/VE for TBM children from the time of initial diagnosis to 12 months after diagnosis.
CONCLUSIONSTidal breathing pulmonary function has characteristic changes in children with TBM. Tidal breathing pulmonary function tends to be recovered with increased age in children with TBM.
Age Factors ; Female ; Humans ; Infant ; Lung ; physiopathology ; Male ; Respiration ; Tidal Volume ; Tracheobronchomalacia ; physiopathology
3.Alterations in cardiac structure and function in a modified rat model of myocardial hypertrophy.
Wen-Jun DAI ; Qi DONG ; Min-Sheng CHEN ; Lu-Ning ZHAO ; Ai-Lan CHEN ; Zhen-Ci LI ; Shi-Ming LIU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(5):626-633
This study was aimed to establish a stable animal model of left ventricular hypertrophy (LVH) to provide theoretical and experimental basis for understanding the development of LVH. The abdominal aorta of male Wistar rats (80-100 g) was constricted to a diameter of 0.55 mm between the branches of the celiac and anterior mesenteric arteries. Echocardiography using a linear phased array probe was performed as well as pathological examination and plasma B-type natriuretic peptide (BNP) measurement at 3, 4 and 6 weeks after abdominal aortic constriction (AAC). The results showed that the acute mortality rate (within 24 h) of this modified rat model was 8%. Animals who underwent AAC demonstrated significantly increased interventricular septal (IVS), LV posterior wall (LVPWd), LV mass index (LVMI), cross-sectional area (CSA) of myocytes, and perivascular fibrosis; the ejection fraction (EF), fractional shortening (FS), and cardiac output (CO) were consistently lower at each time point after AAC. Notably, differences in these parameters between AAC group and sham group were significant by 3 weeks and reached peaks at 4th week. Following AAC, the plasma BNP was gradually elevated compared with the sham group at 3rd and 6th week. It was concluded that this modified AAC model can develop LVH, both stably and safely, by week four post-surgery; echocardiography is able to assess changes in chamber dimensions and systolic properties accurately in rats with LVH.
Animals
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Aorta, Abdominal
;
pathology
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Constriction, Pathologic
;
complications
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Disease Models, Animal
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Echocardiography
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methods
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Enzyme-Linked Immunosorbent Assay
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Heart
;
physiopathology
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Hypertrophy, Left Ventricular
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blood
;
etiology
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pathology
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Male
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Myocardium
;
pathology
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Natriuretic Peptide, Brain
;
blood
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Rats, Wistar
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Time Factors
5.Application of bronchoscopy in 354 children.
Gao-Ping ZHANG ; Jian-Mei LIU ; Qiang CHEN ; Lan LI ; Jian LI ; Xiao-Hua ZHU ; Ai-Min WU ; Ci-Lang HU ; Hua-Feng HU
Chinese Journal of Contemporary Pediatrics 2010;12(3):230-232
6.Application of bacterial cultures of bronchoalveolar lavage fluids in children with pulmonary infection.
Gao-Ping ZHANG ; Qiang CHEN ; Jian-Mei LIU ; Shu-Ping ZHOU ; Xiao-Jun YU ; Juan LU ; Lan LI ; Xiao-Hua ZHU ; Ai-Min WU ; Jian LI ; Ci-Lang HU ; Yun DU ; Li LI
Chinese Journal of Contemporary Pediatrics 2012;14(5):350-352
OBJECTIVETo study the value of bacterial cultures of bronchoalveolar lavage fluids (BALF) in children with pulmonary infection.
METHODSBacterial cultures sampled from both sputum and BALF were performed on 80 hospitalized children with pulmonary infection between June 2008 and February 2011.Culture results between the two samples were compared.
RESULTSIn the 80 children with pulmonary infection, bacterial cultures of BALF showed that Viridans Streptococci were found in 72 cases (90%), Neisseria in 41 cases (51%), Streptococcus pneumoniae in 11 cases (14%), Staphylococcus Aureus in 3 cases (4%) and Escherichia coli in 3 cases (4%). The positive rates of Viridans Streptococci in the bacterial cultures of BALF was not significantly different from the bacterial cultures of sputum, but the positive rate of Streptococcus pneumoniae in the bacterial cultures of BALF was significantly higher than in the bacterial cultures of sputum (4%). Moreover, Escherichia coli were found only by bacterial cultures of BALF.
CONCLUSIONSBacterial cultures of BALF are useful in the identification of pathogenic bacteria for pulmonary infection in children. Due to the samples taken from the lesion regions in bacterial cultures of BALF, the results of may be more reliable.
Bacteria ; isolation & purification ; Bacterial Infections ; microbiology ; Bronchoalveolar Lavage Fluid ; microbiology ; Child, Preschool ; Female ; Humans ; Infant ; Lung Diseases ; microbiology ; Male
7. Betulinic Acid Improves Cardiac Function in Septic Rats Through AKT / mTOR and AKT / AMPK -modulated Autophagy
Xiang-Fei MENG ; Xin-Ru ZHOU ; Jing HUANG ; Meng-Yuan CHEN ; Li-Juan LIU ; Lu-Jia SHI ; Ci-Ai CHEN ; Ling-Bo QIAN
Chinese Journal of Biochemistry and Molecular Biology 2023;39(6):840-847
Betulinic acid (BA) exerts protective effects on organs in septic animals. However, whether BA can improve cardiac function in sepsis and the underlying mechanism remain unclear. Here, male Sprague-Dawley rats were pretreated with BA (25 mg/ kg/ d, i. g.) for 5 days and then intraperitoneally injected with lipopolysaccharide (LPS, 10 mg/ kg). The rats were anesthetized to determine transthoracic echocardiography using a high-resolution imaging system for small animals after they were treated with LPS for 6 h. Histopathologic alterations were examined by HE staining. Myocardial injury markers (cTnI and CK-MB) and inflammatory factors (TNF-α, IL-1β and IL-6) in the serum were measured by the enzyme-linked immunosorbent assay. Autophagy-related proteins (p62 and LC3 Ⅱ) and AKT-modulated autophagy pathways in the myocardium were determined by Western blotting. Pretreatment with BA markedly improved left ventricular ejection fraction (EF) and fraction shortening (FS) (P<0. 05), improved myocardial histomorphology, and significantly inhibited cTnI, CK-MB, TNF-α, IL-1β and IL-6 (P<0. 05) in the septic rat serum. BA markedly decreased p62 (P<0. 01), increased LC3 Ⅱ (P< 0. 001), and significantly down-regulated p-AKT (Thr308), p-AMPKα (Ser485/ 491), p-mTOR (Ser2448) and p-S6K (Thr389) (P<0. 05), while markedly up-regulated p-AMPKα (Thr172) and pULK1 (Ser317) (P<0. 01) in septic rat hearts. The findings indicate that BA can attenuate sepsis-induced myocardial dysfunctions associated with down-regulating autophagy inhibiting pathways mediated by AKT/ mTOR and AKT/ AMPK pathways.
8.Effect of Mobile Health for Standardized Management on Women with Gestational Diabetes Mellitus.
Fei-Ling HUANG ; Hui-Ying HU ; Su-Han ZHANG ; Li LI ; Li ZHANG ; Xiao-Xia CHEN ; Chu-Ci ZHANG ; Hong-Xiu ZHONG ; Ai-Min YAO ; Cui-Ying LIU ; Ning-Zhi ZHANG ; Xiao-Wen XUE ; Liang-Kun MA
Acta Academiae Medicinae Sinicae 2021;43(4):551-557
Objective To explore the performance of mobile health platform for standardized management of pregnant women with gestational diabetes mellitus(GDM). Methods A randomized controlled trial was conducted,in which 295 women with GDM were randomized into two groups(traditional management group and mobile health management group)by a computer-generated sequence.The traditional management group accepted standardized GDM management,and the mobile health management group was supplemented by mobile health management based on the standardized management.The glycemic control rate and the incidences of low birth weight,macrosomia,preterm birth,premature rupture of membranes,postpartum hemorrhage after cesarean section,neonatal asphyxia,malformation,and admission to the neonatal intensive care unit were compared between the two groups. Results The glycemic control rate in mobile health management group was significantly higher than that in the traditional management group [(67.22±22.76)%
Cesarean Section
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Diabetes, Gestational/therapy*
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Female
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Fetal Macrosomia
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Humans
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Infant, Newborn
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Pregnancy
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Pregnancy Outcome
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Premature Birth
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Telemedicine