Objective To observe the effect of acupuncture on brain-derived neurotrophic factor(BDNF) and nerve growth factor(NGF) expression in the hippocampus of depression rats,and to explore its possible anti-depression mechanism.Methods SD rats were randomized into the normal group,model group,acupuncture group and western medicine group.Rat models of depression were established by chronic unpredictable mild stress and by isolated raising.The western medicine group received gastric gavage of fluoxetine hydrochloride(in the dose of 2 mg?kg-1?d-1),and the acupuncture group received acupuncture on acupoints of Baihui(GV20),Xinshu(BL15) and Ganshu(BL17),once per day for 21 days.After treatment,the expression of BDNF and NGF in the hippocampus of rats was detected by immunohistochemical assay.Results The number of cells with BDNF and NGF positive expression in the hippocampus of model group was decreased(P

0.05).After treatment,the nipple height was increased in the model group(P

Objective To evaluate the effect of Tai Chi exercise on cognitive function of the aged.Methods Searched China National Knowledge Infrastructure database,Wanfang data knowledge service platform,Chongqing VIP Chinese science and technology periodical database,China biomedical literature database,PubMed,The Cochrane Library and Embase.Collected the clinical research literature in which the elder population practiced Tai Chi and cognitive function outcomes were researched.Conducted Cochrane bias risk assessment.Conducted systematic review and Meta-analysis for the effective information.Results Included 8 randomized controlled trials in total.All studies were described as random,but only two studies described the random method,with one study with blinding.The overall methodological quality was low.Meta-analysis results showed that,the cognitive function outcome evaluation in the aged with cognitive function defect,the overall cognitive function [MD =0.91,95％ CI (0.37,1.46),I2=0,P=0.001],language fluency [MD=2.17,95％CI (0.88,3.45),I2=0,P＜0.001] and delayed recall test [MD =0.7,95％ CI (0.29,1.11),I2 =0,P ＜0.001] evaluation in the Tai Chi group were significantly better than that in the control group,with no significant improvement in memory span [MD =0.16,95％ CI (-0.14,0.45),I2 =55 ％,P =0.30].In the outcome indicators evaluation of the healthy elder people,the overall cognitive function evaluation [MD =0.7,95％ CI (0.11,1.29),P =0.02] in the Tai Chi group was significantly better than the control group.In language fluency evaluation [MD =0.32,95％ CI (0.01,0.64),I2 =0 P =0.05],the difference was not statistically significant,but there is a potential advantage;in evaluation of execution of test line A (TMT-A) [MD =1.53,95％ CI (0.1,2.96),I2 =26％,P =0.04],the Tai Chi group was better than the control group;in evaluation of execution of test line B (TMT-B) [MD =1.24,95％CI (-5.20,7.67),I2 =94％,P =0.71] the difference between the Tai Chi group and the control group was not significant.Conclusion Tai Chi could effectively improve cognitive function of the healthy elder people and those with cognitive impairment.

Objective To observe the therapeutic effect of suspending-needle therapy combined with modified Zhengan Xifeng Decoction for the treatment of primary facial spasm,and to explore an effective traditional Chinese medicine therapy for primary facial spasm.Methods A total of 65 qualified patients were randomly divided into trial group (33 cases) and control group(32 cases),which was treated with suspending-needle therapy combined with modified Zhengan Xifeng Decoction,Carbamazepine tablets respectively.Twenty-eight days constituted one course of treatment.After treatment for one course,therapeutic efficacy of the two groups was evaluated,and the degree of facial spasm was assessed.Results (1) After one course of treatment,the markedly-effective rate of the trial group was 84.8％,and that of the control group was 62.5％,the difference being significant(P ＜ 0.05).(2) After treatment,the degree of facial spasm in the two groups was much improved as compared with that of the control group (P ＜ 0.05 or P ＜ 0.01),and the improvement in the trial group was superior to that in the control group (P ＜ 0.01).Conclusion Suspending-needle therapy combined with modified Zhengan Xifeng Decoction has better effect on relieving facial spasm of the primary facial spasm patients than Carbamazepine tablets.

Objective To observe and analyze the standards of the normal vivo renal multi-slice spiral CT(MSCT) imaging to provide relevant normal range for clinic and teaching.Methods 123 cases with normal kidneys were undergone abdominal MSCT,then 3D imaging was performed,the position of kidney distance between two poles of kidney and the central line of the spine,renal length and short axis,the angels between the renal length axis and sagittal segment(LS) and the coronal segment(LC),the angel between coronal segment and the longest distance line from the midpoint of the renal gate to the same kidney's out edge(CL) were observed and measured with statistics analysis.Results Mostly the position of left kidney was higher than that of the right.Almost kidney's upper poles located at the T12 level.The number of left kidney was more than the right kidney in the renal upper pole located higher than T11.Almost kidney's inferior poles located at from the L2 inferior edge level to the L3 inferior edge level.In the statistic results,the values measured on CT of left kidney were large or equal to the right except for CL and LC in men,LC in women,total LC and LS.The width in men,total length and width and total upper distance were of significant statistics meaning(P

Objective To observe the effect of electroacupuncture(EA) therapy of activating Du meridian,regulating mind and reinforcing the fundamental on learning and memory ability and plasma endothelin(ET) level in rats with vascular dementia(VD).Methods Healthy adult SD rats were randomized into pseudo-operation group(N=8),EA group(N=9,EA on the acupoints of Baihui,Dazhui,Pishu and Shenshu),nimodipine group(N=8,at the dose of 12mg/kg) and the model group(N=8).Rat models of VD were established by the modified 4-vascular occlusion(4-VO)method.Morris water maze test was used for the behavioral observation,and plasma ET level was measured by radioimmunoassay(RIA).Results Plasma ET level was increased obviously,the disorder of learning and memory ability was obvious and the escape latency in water maze was prolonged in the model group(P0.05).In EA group and nimodipine group,the escape latency was shortened(P0.05 compared with the pseudo-operation group).Conclusion The therapeutic mechanism of EA therapy of activating Du meridian,regulating mind and reinforcing the fundamental for VD is probably related with the regulation of plasma ET level and with the increase of learning and memory ability.

[Objective] To study the relationship between syndrome patterns of traditional Chinese medicine and bcl- 2 oncogene, p53 suppression gene mRNA expressions in precancerous lesions of gastric cancer (PIGC). [Methods] Forty PLGC cases were endoscopically and pathologically confinned, including 24 cases of moderate dysplasia of gastric mucosa, 9 cases of severe dysplasia, 7 cases of incomplete colonic intestinal metaplasia. Of the 40 cases, 10 were complicated with Qi stagnation, 12 with stomach-heat, and 18 with blood stasis, mRNA expression of bcl- 2 oncogene and p53 suppression gene were detected in situ by molecular hybridization method. [Results] The mRNA overexpression of bcl- 2 oncogene and p53 suppression gene were found in PLGC, and the expression was gradually increased with the progress of lesions. In the complicated cases, the mRNA expression of bcl- 2 oncogene and p53 suppression gene were the least in the cases with Qi stagnation and less in the cases with stomach- heat than in the cases with blood stasis. [Conclusion] Abnormal mRNA expression of bcl- 2 oncogene and p53 suppression gene were found in PLGC cases and related with different complicated cases, indicating the specificity of different syndrome patterns.

[ Objective ] To observe the therapeutic effect of acupoint thread-embedding therapy for chronic gastritis. [Methods] Seventy cases of chronic gastritis were randomized into group A ( n = 36, treated with acupoint thread-embedding therapy) and group B (n = 34, treated with acupuncture); the changes of electrogastrogram (ECG) and plasma contents of gastrin (Gas) and substance P (SP) were compared before and after treatment. [Results] The effective rate was 88.89% in group A and 76.47% in group B, the difference being significant ( P 0.05 ) while postprandial ECG frequency differed from that before treatment ( P 0.05). [Conclusion] Acupoint thread-embedding therapy exerts certain effect for the treatment of chronic gastritis; its therapeutic mechanism may be related to the regulation of gastrointestinal function by regulating gastrointestinal hormone levels and by increasing ECG amplitude and frequency.

BACKGROUND: It is thought in traditional Chinese medicine that dogday moxibustion (DDM) has good clinical effect in treating bronchial asthma. Study of its influence on soluble cellular adhesion molecules in patients with bronchial asthma can help to well understand its mechanism.OBJECTIVE: To observe the effect of dog-day on soluble cellular adhesion molecules in patients with bronchial asthmaDESIGN: Self-control, case-control observationSETTING: College of Acupuncture and Manipulation and Clinic of the Department of Acupuncture and Moxibustion of the First Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine.PARTICIPANTS: Totally 28 patients with bronchial asthma receiving dog-day moxibustion in the Clinic of the Department of Acupuncture and Moxibustion, First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine from July to August 2004 were set as dog-day moxibustion group, and 10 concurrent health volunteers were set as normal control group.METHODS: According to the principle of "treating winter diseases in summer", the Chinese medicine which consists of such herbs as Baijiezi(Semen Sinapis Albae ), Mahuang(Herba Ephedrae ), Xixin (Herba Asari ),Gans ui (Radix Kansui ), Yanhu (Rhizoma Corydalis ) was stuck on the points such as Feishu, Dingchuan, Fengnen, Bailao, Shenshu. Point selection was at the two sides, and a lumpy salve was stuck on the point with adhesive tape. Three to six hours for adult and one to two hours for children in each sticking duration; and the operation was respectively done on the first (July20), second (July 30) and third (August 9) dog-day.MAIN OUTCOME MEASURES: Short-term effect 3 months later was observed, the levels of soluble cellular adhesion molecules and IgE were assayed before and 10 days after treatment, and comparison with normal control group was performed. The indexes of the patients before treatment were performed Pearson correlation analysis.RESULTS: Twenty-eight patients and 10 healthy persons all entered result analysis. [1] Intercellular adhesion molecule-1 level was significantly higher before treatment in dog-day moxibustion group than that after treatment in the dog-day moxibustion group and that in the normal control group [(281.39±64.58 ), (256.95±44.22), ( 194.30±39.05 ) μg/L,P ＜ 0.01].[2]Vaso-cellular adhesion molecule-1 level was significantly higher before treatment in the dog-day moxibustion group than that after treatment in the dog-day moxibustion group and that in the normal control group [(234.79±89.75), (185.80±84.41), (128.85±51.28)μg/L,P ＜ 0.01]. [3]IgE level was significantly higher before treatment than that after treatment in the dog-day moxibustion group and that in the normal control group [(482.61±123.43), (418.96±134.18), (161.53±43.64)μg/L,P＜ 0.01]. [4]Pearson correlation analysis result: Intercellular adhasion molecule-1 level is significantly positively correlated with IgE and vascular intercellular adhesion molecule-1 level (r =0.641,0.449 ,P ＜ 0.01,0.05). [5]Effect: Total effective rate was 93% (26/28)in the treatment of bronchial asthma with dogday moxibustion . The type of the symptoms, with blister or not, course of disease and condition of disease had no significant influence on effect (P＞ 0.05).CONCLUSION: Dog-day moxibustion may effect through inhibiting the expression of adhesion molecule or inhibiting leukocyte to adhere endothelial cell directly so as to reduce the infiltration of inflammatory cells.

【Objective】 To observe the effect of acupuncture on immune organs(thymus and spleen) and T lymphocyte subtypes of CD3,CD4 and CD8 in rats with dysmenorrhea.【Methods】Thirty SD rats were randomized into normal control group,model group and electroacupunture(EA) group.Except the normal control group,rats in other groups received intraperitoneal injection of diethylstilbestrol and oxytocin to induce dysmenorrhea.EA group received EA on bilateral acupoints of Sanyinjiao and Guanyuan additionally.After 10-day treatment,body-stretching rate,the pathological changes of thymus and spleen,and blood CD3,CD4 and CD8 levels in the three groups were observed.【Results】In the model group,body-stretching rate was 100%,the pathological changes of thymus and spleen were obvious,and blood CD3 and CD4 levels were decreased as compared with the normal control group(P