Biomedical Research ; Heart Rate ; Humans ; Pneumoconiosis ; physiopathology

Objective To explore the clinical significance of plasma fibrinogen(FIB)and plasma D-dimer (D-D)changes involved in lung cancer patients before and after interventional therapy.Methods 23 patients with lung cancer,27 cases of normal control group collected at the same time,to compared the difference of plasma FIB between patients with lung cancer and the normal control group.All enrolled patients were treated with bronchial artery infusion chemotherapy.Plasma FIB and D-D of all patients were meas-ured before the first and the third interventional therapy.Results Plasma FIB value of patients before interventional therapy was sig-nificantly higher than that of the normal control group and the group after interventional therapy.The difference between the group after interventional therapy and the normal control group was no significant;Plasma D-D value of patients before and after interven-tional therapy was significant.Conclusion Changes of plasma FIB and D-D before and after interventional therapy in patients with lung cancer are significantly correlated with the severity of the disease and interventional therapy efficacy,which can be used as a ver-y meaningful indicator of interventional therapy.

The feasibility and steps of the designing experiments performed by college students in preventive medical speciality were explored in this study,which showed that the designing experiments played an important role in developing students' creativity and their abilities to practice.This kind of experiment is worth extending.

Objective To observe the therapeutic effect of suspending-needle therapy combined with modified Zhengan Xifeng Decoction for the treatment of primary facial spasm,and to explore an effective traditional Chinese medicine therapy for primary facial spasm.Methods A total of 65 qualified patients were randomly divided into trial group (33 cases) and control group(32 cases),which was treated with suspending-needle therapy combined with modified Zhengan Xifeng Decoction,Carbamazepine tablets respectively.Twenty-eight days constituted one course of treatment.After treatment for one course,therapeutic efficacy of the two groups was evaluated,and the degree of facial spasm was assessed.Results (1) After one course of treatment,the markedly-effective rate of the trial group was 84.8％,and that of the control group was 62.5％,the difference being significant(P ＜ 0.05).(2) After treatment,the degree of facial spasm in the two groups was much improved as compared with that of the control group (P ＜ 0.05 or P ＜ 0.01),and the improvement in the trial group was superior to that in the control group (P ＜ 0.01).Conclusion Suspending-needle therapy combined with modified Zhengan Xifeng Decoction has better effect on relieving facial spasm of the primary facial spasm patients than Carbamazepine tablets.

Objective To investigate the therapeautic effects of ulinastatin on ulcerative colitis(UC) and it's possible mechanism.Methods Forty adult Wistar rats were randomly divided into four groups;normal group(group A),UC group(group B),SASP(salicylazosulfapyridine) therapeautic therapeutic group(group C),ulinastatin(UTI) therapeautic group(group D). UC models were induced by trinitrophenylmethylnitramine(TNBS), Group A and Group B received 0 9% saline solution instead of ulinastatin and SASP as a control.Serum level of tumor necrotic factor-?(TNF-?),malondialdehyde(MDA),superoxide dismutase(SOD) and pathological change were measured respectively after drugs treatment for two weeks.Results Both UTI and SASP decreased the level of serum TNF-?,MDA ,increased the level of serum SOD ,and relieved mucosa lesion and pathological change in UC rats . UTI was better than SASP in the treatment of UC.Conclusions UTI has apparent therapeautic effects.The mechanism may be associated with inhibition of pro-inflammatory cytokines and clearance of free radicals.

OBJECTIVE:To explore the differences of plasma concentration of norvancomycin by HPLC and microbiological method. METHODS:Microbiological method and HPLC were used to detect the plasma concentration of norvancomycin,and clinical test result of both techniques was retrospectively analyzed. RESULTS:There were no significant differences in the plasma concentra-tion of norvancomycin by microbiological method and HPLC(y=0.992 7x+0.155 8,r=0.997 6)(P>0.05). CONCLUSIONS:Both microbiological method and HPLC are more effective and reliable for the plasma concentration detection of norvancomycin. The hospi-tals can choose corresponding method according to their condition when determining plasma concentration of norvancomycin.

Objective To study the signs and the value of CT enhanced scanning in diagnosing the liver cancer combined arteriovenous fistulae(AVF).Methods 50 cases of the liver cancer combined AVF had plain and enhanced scanning by spiral CT,then proved by hepatic arteriography.The signs of CT and hepatic arteriography were contrast analyzed.Results CT scan showed 17 cases of artery-portal vein fistulae (centre),10 cases of artery-the small branch of portal vein fistulae(periphery),3 cases of artery-liver vein fistulae,5 cases combined the signs aforementioned,2 cases combined the artery-portal vein fistulae and the artery-liver vein fistulae,and the 13 cases no the characteristic signs of CT,37 cases of the signs of AVF were found by CT,sensitivity 74%.26 cases were diagnosed as AVF,postitive rate 52%.Conclusion The development of portal vein and/or the inferior caval vein ahead in artery phase,especially the parenchyma of the lobes,segments and subsegments of liver,may be a foundation of the diagnosis of AVF.And we discussed the limitation of the CT examination in diagnosing this complication.

Objective: To explore the relationships between blood levels of osteopontin (OPN), brain natriuretic peptide (BNP) and cardiac function in patient with degenerative heart valve disease (DHVD). Methods: Our research included in 2 groups: DHVD group,n=120 relevant patients treated in our hospital from 2013-12 to 2015-02 and Control group,n=30 healthy subjects from physical examination in the same period of time. Based on blood levels of OPN, DHVD patients were further divided into 2 sub-groups as Normal OPN sub-group, the patients with 18.8 ng/ml ≤OPN≤ 30.0 ng/ml,n=35 and High OPN sub-group, the patients with OPN>30.0 ng/ml,n=85. OPN levels at prior treatment (T0) and 3 days (T1), 1 week (T2), 2 weeks (T3) after treatment were compared between DHVD group and Control group; BNP levels, cardiac outcome (CO), cardiac index (CI) and left ventricular ejection fraction (LVEF) were also compared. The relationships between blood levels of OPN, BNP and cardiac function in DHVD patients were studied by Pearson correlation analysis. Results: Compared with Control group at T0 time point, High OPN sub-group showed increased blood levels of OPN and BNP, while decreased CO, CI and LVEF. Compared with Normal OPN sub-group, High OPN sub-group had the higher levels of OPN and BNP at all 4 time points, while lower levels of CO, CI and LVEF. In DHVD group, compared with T0 time point, OPN and BNP levels were decreased at T2 and T3 time points, while CO, CI and LVEF were increased, allP<0.05. Pearson correlation analysis presented that in DHVD patients, blood levels of OPN were positively related to BNP (r=0.936,P=0.00) and negatively related to CO, CI and LVEF (r=-0.869,r=-0.884 andr=-0.858 respectively, allP=0.00). Conclusion: DHVD patients had increased blood level of OPN which is related to BNP level and cardiac function; this might be because of OPN promoting heart valve calciifcation, inlfammatory reaction and myocardial injury. OPN could be used as a reference index for evaluating the cardiac function in DHVD patients.

[ Objective ] To observe the therapeutic effect of acupoint thread-embedding therapy for chronic gastritis. [Methods] Seventy cases of chronic gastritis were randomized into group A ( n = 36, treated with acupoint thread-embedding therapy) and group B (n = 34, treated with acupuncture); the changes of electrogastrogram (ECG) and plasma contents of gastrin (Gas) and substance P (SP) were compared before and after treatment. [Results] The effective rate was 88.89% in group A and 76.47% in group B, the difference being significant ( P 0.05 ) while postprandial ECG frequency differed from that before treatment ( P 0.05). [Conclusion] Acupoint thread-embedding therapy exerts certain effect for the treatment of chronic gastritis; its therapeutic mechanism may be related to the regulation of gastrointestinal function by regulating gastrointestinal hormone levels and by increasing ECG amplitude and frequency.

BACKGROUND: To establish experimental animal model for rapid evaluation of combined wound with seawater immersion so as to benefit wound healing and recovery.OBJECTIVE: To explore the plan of simple evaluation of combined wound with seawater immersion.DESIGN: Group division and controlled experiment was designed.SETTING: Second Department of Orthopaedics, the 113 Hospital of Chinese PLA; Research Institute of Surgery, Daping Hospital, Third Military Medical University of Chinese PLA MATERIALS: Totally 32 health hybridized adult dogs were employed,mass weighted varied from 12 to 15 kg, of either sex.METHODS: The experiment was performed partially in the specific sea area of the Fifth and Sixth Room , Research Institute of Surgery of Daping Hospital of the Third Military Medical University of Chinese PLA from October 2001 to June 2002. The dogs were randomized into burn-blast combined wound group and projectile-burn combined wound group and each group was subdivided into immersion group (10 dogs) and the control (6 dogs). Burn-blast combined wound group: The model of burn and mild blast injury was prepared. In the control, the dogs were put in a room at 25 ℃ directly after injury; But in immersion group, the dogs were immersed immediately in simulated seawater with holder. Projectile-burn combined wound group: The model of burn and mild projectile injury was prepared. The managements were same as the previous after injury in the control and immersion group. The change of physiologic indexes was observed in 28 hours after injury. The indexes related to death were picked up for correlative analysis and establishing wound scoring scale, inducing body temperature, heart rate, mean arterial pressure (MAP), chloride concentration, PaO2 and causative factor, 0-5 score were designed, the higher the score was, the higher mortality would be.MAIN OUTCOME MEASURES: ① Analysis of dead cases in each group. ② Wound scoring. ③ Verification of wound in scoring scale.RESULTS: 32 dogs all entered result analysis. ① Analysis of dead cases and relationship: Totally, 12 dogs were died. The peak of death was in 10-12hours, no matter the immersion happened or not. 5 indexes were associated significantly with death, named body temperature, heart rate, MAP, PaO2 and chloride concentration (P ＜ 0.05). ② Comparison of wound scoring, predictive mortality and actual mortality in each group: The results in burn-blast control were (5.89±3.25) score, 0 and 17% successively. Those in projectileburn control were (6.78±2.02), 25% and 33% successively. Those in burn blast immersion group were (9.50±4.20) scores, 50% and 30% and those in projectile-burn immersion group were (11.13±3.57) scores, 50% and 60%.CONCLUSION: ① The established scoring scale is apt to obtain common physiological indexes so as to evaluate the wound rapidly, which is applica ble for either seawater immersed cases or non-immersed cases, indicating extensively applications. ② The correction of evaluation is acceptable with verification. ③ The scale is based on animal experimental data, for which,it is still different from the one of real wound evaluation.