OBJECTIVEwe aimed to investigate the mutation and expression of BRAF gene in mature T/NK cell lymphoma tissues and cell lines, explore the correlation between gene alterations and clinicopathological features and clinical outcomes of mature T/NK cell lymphoma.

METHODSFirstly, we detected common mutant sites of BRAF (locus 1 799 mutation in exon 15 and loci 463, 465 and 468 mutation in exon 11) in lymphoma Jurkat, Hut-78 and YTS cell lines, normal peripheral blood lymphocytes, different types of mature T/NK cell lymphoma and reactive hyperplasia lymph nodes by direct sequencing. Then we measured the expression of BRAF in Jurkat, Hut-78, YTS cells and normal peripheral blood lymphocytes by real time-PCR and Western-blot detection. We also used immunohistochemistry (IHC) to detect the expression of BRAF in mature T/NK cell lymphoma tissues and reactive hyperplasia lymph nodes, and to analyze the correlation between the expression of BRAF and clinocopathological features and clinical outcomes.

RESULTSWe did not find common BRAF mutation in mature T/NK cell lymphoma tissues and cell lines, and the relatively expression of BRAF gene mRNA in normal peripheral blood lymphocytes, YTS, Hut-78 and Jurkat cells were 1.000, 5.207±0.013, 8.412±0.615 and 36.720±1.797, respectively, and protein expressions were 0.051±0.003, 0.102±0.013, 0.113±0.017 and 0.304±0.010, respectively, and the expression of BRAF in peripheral T cell lymphoma Jurkat cells was significantly higher than that of Hut-78, YTS cells and normal lymphocytes (P<0.05). Only 6 of 58 peripheral T cell lymphomas (10.3%) had positive BRAF expression, and were the subgroups of peripheral T cell lymphoma-unspecified type. The statistical data did not show any correlation between positive expression of BRAF and gender, age, clinical stage, location, lactate dehydrogenase in the 21 cases of peripheral T cell lymphoma-unspecified type (P<0.05), but the positive rate of BRAF in the effective treatment group (8.3%) was significantly lower than that of the invalid group (55.6%, P<0.05).

CONCLUSIONThe expression of BRAF gene may become a marker of malignant biological characteristics and clinical therapeutic target of peripheral T cell lymphoma.

Exons ; Humans ; Immunohistochemistry ; Killer Cells, Natural ; Lymphoma, T-Cell, Peripheral ; genetics ; metabolism ; pathology ; Proto-Oncogene Proteins B-raf ; genetics ; metabolism ; Pseudolymphoma ; genetics ; metabolism ; RNA, Messenger ; metabolism

Animals ; Cell Division ; drug effects ; Cells, Cultured ; Drugs, Chinese Herbal ; pharmacology ; Growth Inhibitors ; pharmacology ; Hepatocytes ; cytology ; drug effects ; Lamiaceae ; chemistry ; Rats

Objective To investigate the impacts of different body posture care in 6 hours after operation in the ankylosing spondylitis ( AS) patients undergoing osteotomy surgery. Methods A total of 92 AS patients who underwent osteotomy surgery with regained consciousness and stable vital signs were randomly divided into experimental and control groups (46 patients of each group). The patients in the experimental group were sent back to ward after operation and they were helped to lie on the left or right side, and the patients in the control group were helped to lie on the back in the 6 hours after operation. Patients′ occurrence of vomiting, dizzy, rate of heart and respiratory, occurrence of stage-I pressure sores and tension vacuole, visual analogue scale ( VAS) of abdominal skin and back incision were observed and recorded. Results The incidence of vomiting, dizziness andⅠperiod pressure ulcer in experimental group were 10. 9%, 6. 5%, 2. 2%, compared with 15. 2%, 4. 3%, 0. 0% in the control group, which had no statistically significant differences (χ2 =0. 383, 0. 212, <0. 001; P >0. 05). Patients′ postoperative mean breathing rate within 6 h after surgery in the experimental group was [(19. 3 ± 3. 4) times/min] comparing with [(20. 4 ± 4. 1) times/min] in the control group, and there was no statistically significant difference (t = 1. 401, P > 0. 05). Patients′ heart rate [(92.5±6.3)times/min]andtheincidenceofabdominaltensionblisters(4.3%)intheexperimentalgroup were significantly lower than that in the control group [(118. 2 ± 7. 8) times/min, 17. 4%] ( t =17. 385,χ2 =4. 039;P<0. 05). There were statistically differences in patient′s abdominal pain and back incision VAS score distribution in two groups(u =2. 022,2.218;P <0. 05). Conclusions After osteotomy surgery, the application of general anesthesia for AS kyphosis, with regaining consciousness and stable vital signs, applying preoperative habits of lateral position in 6 hours after being sent back to ward, can relieve pain, decrease the occurrence of tension vacuole, prompt patients′comfort as well as accelerate recovery.